Evaluation of Ruminal Dry Matter Disappearance and pH of Dry Corn, High-Moisture Corn, and Rye under in Vitro Conditions

An in vitro experiment was conducted to evaluate the effects of grain type on in vitro dry matter disappearance (IVDMD) and pH using ruminal fluid and a buffer reagent. Five beef cows were used for ruminal fluid collection and ruminal fluid was pooled prior to use. The cows used for ruminal fluid collection were maintained on a forage-based diet (60:40 forage to concentrate) for 28 d prior to ruminal fluid collection. Three grain types were evaluated: 1) dry corn (89% dry matter;DRC), 2) high-moisture corn (72% dry matter;HMC) and 3) rye (89% dry matter;RYE). Data were analyzed as a completely randomized design. A total of twenty-one replications of each grain type were used for statistical analysis (n = 63 tubes total). IVDMD was greater (P ≤ 0.05) by 97.1% and 46.4% for RYE compared to DRC and HMC, respectively. Additionally, IVDMD was greater (P = 0.05) by 34.6% for HMC compared to DRC. Final pH was decreased (P ≤ 0.05) for RYE by 12.3% and 2.8% for RYE compared to DRC and HMC, respectively. Also, final pH was decreased (P = 0.05) by 9.8% for HMC compared to DRC. These data indicate that corn harvest method (dry vs. high-moisture) influences IVDMD and final pH and that rye has a greater disappearance of dry matter and lower final pH than corn under in vitro conditions.

Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance

Rye(Secale cereale L., 2n=2x=14, RR) is a significant genetic resource for improving common wheat because of its resistance to multiple diseases and abiotic-stress tolerant traits. The 1RS chromosome from the German cultivated rye variety Petkus is critical in wheat breeding. However, its weakened disease resistance highlights the need to identify new resources. In the present study, a novel derived line called D27 was developed from common wheat and Mexico Rye.Cytological observations characterized the karyotype of D27 as 2n=42=21 Ⅱ. Genomic in situ hybridization indicated that a pair of whole-arm translocated Mexico Rye chromosomes were inherited typically in the mitotic and meiosis stages of D27. Experiments using fluorescence in situ hybridization(FISH) and gliadin electrophoresis showed that D27 lacked wheat 1DS chromosomes. They were replaced by 1RS chromosomes of Mexico Rye, supported by wheat simple-sequence repeat markers, rye sequence characterized amplified region markers, and wheat 40K SNP array analysis.The wheat 1DS chromosomes could not be detected by molecular markers and wheat SNP array, but the presence of rye 1RS chromosomes was confirmed. Agronomic trait assessments indicated that D27 had a higher tiller number and enhanced stripe rust and powdery mildew resistance. In addition, dough properties analysis showed that replacing 1DS led to higher viscosity and lower dough elasticity in D27, which was beneficial for cake making. In conclusion, the novel cytogenetically stable common wheat–Mexico Rye T1DL·1RS translocation line D27 offers excellent potential as outstanding germplasm in wheat breeding programs focusing on disease resistance and yield improvement. Additionally,it can be valuable for researching the rye 1RS chromosome’s genetic diversity.

Establishment and Optimization of Rye-specific PCR Reaction System

[Objective] The aim was to establish the optimal rye-specific PCR reaction system for rye.[Method] The ordinary wheat ＂Chinese Spring＂,S165,rye,octoploid triticale and hexaploid triticale were used as materials to carry out study on the effect of the amount of template DNA,primers,dNTPs,Mg2＋ concentrations,Taq DNA polymerase and annealing temperature on the rye-specific PCR reaction system of rye.[Result] The genomic DNA extracted by modified CTAB DNA extraction method showed high quality,which was satisfied for the PCR reaction template.The rye-specific PCR reaction system was 25 μl,including 10 × buffer solution,1.5 mmol/L MgCl2,200 μmol/L dNTP,40 ng primers,40-60 ng DNA template and 1 U Taq DNA polymerase.[Conclusion] The optimal rye-specific PCR reaction system was established,which provided basis for the identification of exogenous germplasm of rye in wheat background.

Antifungal Activity of Lactic Acid Bacteria, Isolated from Bulgarian Wheat and Rye Flour

The economic losses and the health hazards of the mycotoxins produced by spoilage fungi are the main concerns of the food industry. The spoilage of bakery products by fungi is more common in countries with a high humidity and temperature. About 5-10% of food production is spoiled by the growth of yeast and fungi in food materials. Similarly, in Western Europe, the growth of the spoilage fungi of bread is estimated to reach more than 200 million Euros per year. The history conditions of the food can be a major factor in determining any fungal spoilage--for example, stored and processed foods are more sensitive to spoilage when compared with fresh and prepared foods. Lactic acid bacteria isolated from Bulgarian wheat and rye flour were used in the present study to check their antifungal properties against pathogenic yeast and fungi imperfecta using standard disc diffusion method in vitro. A broad spectrum of antifungal activity of the six newly identified as L. plantarum strains e Tsl, Ts2, Ts3,Ts4 and Ts5, and L. helveticus Ts6 was estimated. Our in vitro studies were performed with wheat and rye sourdough, in order to simulate a real product and to assess the bio-protective potential of the tested lactobacilli. The used test-cultures are representatives of carcinogenic, toxigenic, deteriorative and allergenic fungi from the genera .4spergillus and Penicillium. The all tested strains completely suppress the growth of against C. glabrata 72. Strains L. plantarum Tsl and Ts3 completely suppress the growth against S. cerevisae. While, in the sample with L. plantarum strains e Tsl, Ts2, Ts3,Ts4 and Ts5, and L. helveticus Ts6, a retarded and weak growth of A. niger and P. claviforme was observed. However, the spore germination and the colony growth started only on the fifth day of the mould lactobacilli co-cultivation, which also should be considered as a good result. In this study six isolates Tsl,Ts2, Ts3, Ts4, Ts5 and Ts6, from the traditional Bulgarian wheat and rye flour have been identified as L. plantarum and L. helveticus and characterized as cultures with promising antifungal activity. Obtained results from the combined molecular identification （16S rRNA gene sequencing） approach contribute to give new data on the microbial biodiversity of this not well-studied niche. The antifungal activity of our new isolates, identified as L. plantarum and L. helveticus, seems to be a promising advantage of these six strains, suggesting their potential applications in different food technologies. However, more experiments have to be conducted to clarify the nature and the mechanisms of the reported antifungal activity and they are still in progress. The combination of dairy origin and strong inhibitory activity of the lactobacillus strains is a prerequisite for their possible application as starters and/or bioprotective antifungal adjuncts.

A Brief Discussion about the Creating and Portraying of Holden Caulfield in The Catcher in the Rye

The Catcher in the Rye,J.D. Salinger's first long fiction,was one of his most famous and successful works. It not only influenced American novels written afterwards,for example,Evan Hunter's Last Summer,Sylvia Plath's The Bell Jar,but also influenced the American society. Take the American film industry which was so good at chasing the audience's interest for a typical example:a large numebr of movies,such as Rebel Without A Cause,American Graffiti,Dirty Dancing,The graduate,and so on:they wouldn't have been filmed like that if The Catcher in the Rye didn't go front as a model. Then,can we really find any big differences between the anxiety ex-pressed by Holden and that by the music and words of Jewel,the Green Day,and the Smashing Pumpkins?

No Place for Adolescence to Rest——The Symbolic Scenes on Holden's Journey in The Catcher in the Rye

By releasing the book The Catcher in the Rye,J.D.Salinger received an immediate popularity of his writing career.Hissymbolic use of language has been thoroughly researched but the symbolic scenes which make up Holden's life stage,especiallythe symbolic connotations of ironic resting places in the novel,such as bed,couch and bedroom,has not been paid much attention.It tries to analyse the four scenes: on Holden's history teacher's bed,on the hotel bed with a prostitute,in his sister's bedroom,and on his English teacher's couch,and aims to discover his spiritual chaos as well as adolescent desires in the real world,demon-strating that there is no place for adolescent Holden to rest after he chooses his own stage of scenes in his life.

Low-FODMAP vs regular rye bread in irritable bowel syndrome:Randomized SmartPill~ study

AIM To compare the effects of regular vs low-FODMAP rye bread on irritable bowel syndrome(IBS) symptoms and to study gastrointestinal conditions with Smart Pill?.METHODS Our aim was to evaluate if rye bread low in FODMAPs would cause reduced hydrogen excretion,lower intraluminal pressure,higher colonic p H,different transit times,and fewer IBS symptoms than regular rye bread.The study was a randomized,double-blind,controlled cross-over meal study.Female IBS patients(n = 7) ate study breads at three consecutive meals during one day.The diet was similar for both study periods except for the FODMAP content of the bread consumed during the study day.Intraluminal p H,transit time,and pressure were measured by Smart Pill,an indigestible motility capsule.RESULTS Hydrogen excretion(a marker of colonic fermentation) expressed as area under the curve(AUC)(0-630 min) was [median(range)] 6300(1785-10800) ppm?min for low-FODMAP rye bread and 10 635(4215-13080) ppm?min for regular bread(P = 0.028).Mean scores of gastrointestinal symptoms showed no statistically significant differences but suggested less flatulence after low-FODMAP bread consumption(P = 0.063).Intraluminal pressure correlated significantly with total symptom score after regular rye bread(ρ = 0.786,P = 0.036) and nearly significantly after lowFODMAP bread consumption(ρ = 0.75,P = 0.052).We found no differences in p H,pressure,or transit times between the breads.Gastric residence of Smart Pill was slower than expected.Smart Pill left the stomach in less than 5 h only during one measurement(out of 14 measurements in total) and therefore did not follow on par with the rye bread bolus.CONCLUSION Low-FODMAP rye bread reduced colonic fermentation vs regular rye bread.No difference was found in median values of intraluminal conditions of the gastrointestinal tract.

Effect of Mercury Deposition on Mercury Content and Distribution in Rye Grass

A grass (Italian rye grass) experiment with complete design of three factors (Hg, grass and exposure) andtwo levels (with and without the factor tested) was carried out in Goteborg, Sweden from May to September,1995, toward understanding the effect of mercury deposition on plant Hg conteot and its distribution. It hasbeen foulld that besides the two general pathways of Hg absorption, i.e. root uptake and foliar absorption,mercury deposition can also significantly increase Hg content and total load in grass plant. Such effectsseem to be condned only within the above-ground parts of grass plant. Estimate has been made to makeclear of the contributions of different pathways to Hg content and total Hg load in the upper part of grass.Results showed that the coDtribution of Hg deposition accounted for 27%-32% of Hg content in the above-ground parts of grass plant without adding Hg to the soil, decreasing with the increase of soil and/or air Hgconcentrations. The increment of Hg load in the upper part of grass plant caused by Hg deposition duringan interval of two weeks varied between O.o1~O.o7 pg pot-1, contributing to 17%~48% of the total Hg loadin grass plant exposed.

Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of wholemeal water extract

The grain of rye(Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides,making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract.However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of m RNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides,whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharidedegrading enzymes.

Allelopathic Effects of Cereal Rye on Weed Suppression and Forage Yield in Alfalfa

Cereal rye (Secale cereale L.) is widely used as cover crop because of its allelopathic effects and effectiveness in weed suppression. In the Southeastern US, rye is traditionally grown for winter grazing in dormant bermudagrass pastures, where alfalfa (Medicago sativa L.) is increasingly planted as a companion crop. The effect of cereal rye on alfalfa as a succeeding crop is not known. Therefore, the objective of this study was to evaluate the effect of cereal rye on alfalfa seedling emergence, growth, forage yield, and weed suppression in field conditions. Rye was planted in the fall (mid-October) and the biomass was harvested in spring (March) followed by disking and incorporation of the remaining stubble in the soil. Alfalfa seed was planted four weeks later. The experiment design was a split-plot design with the main plots being no-rye and after-rye and the sub-plots being alfalfa cultivars. Ten alfalfa cultivars were planted in three replications after-rye and three replications with no-rye as a previous crop. In the establishment year, weed density was significantly (p < 0.01) lower in the after-rye alfalfa plots by nearly 77%. Alfalfa seedling counts were also significantly lower (p 0.01) among the cultivars planted in the after-rye block compared to the no-rye, with a seedling count reduction between 35% and 64%. Reduction in total dry biomass yield varied from 15% to 43% among the cultivars planted in the after-rye block. The results of this study also suggest that the allelopathic effect of rye on alfalfa may not persist beyond the establishment season, but the enormous yield reduction in the first production season may constitute a costly economic penalty in terms of forage production. There was variation in the response of different alfalfa cultivars to the effect of rye residue as indicated by the variation in the magnitude of reduction in stand count and forage yield. This warrants more research in multi-location trials with and without rye in order to establish whether there is genetic variation in alfalfa germplasm in their tolerance to cereal rye allelopathy.

Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines

The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding.Crosses belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen source.The percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and pAs1.FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

Yield and Quality Response of Ryegrass, Egyptian Clover and Their Mixtures to Different Sources of Fertilizers

Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilization treatments and their combination to increase forage yield and quality grown under sandy soil conditions. The experiment included the combination of five mixing ratios (Egyptian clover alone, ryegrass alone, 75% Egyptian clover: 25% ryegrass, 50% Egyptian clover: 50% ryegrass and 25% Egyptian clover: 75% ryegrass) and eight fertilizer sources, which include control, organic fertilization, bio fertilization, chemical fertilizer, organic + bio fertilizer, organic + chemical fertilizer, bio + chemical fertilizer and combination of organic and chemical and bio fertilizers. The obtained results indicate the superiority of 75% E. clover: 25% ryegrass mixture fertilizedby Bio + O + N in fresh and dry forage production. On the other hand, it reported the lowest dry weight of weeds g/m2. Chemical analysis of forage plants showed that the mixture of ?75% E. clover: 25% ryegrass surpassed that of other treatments yield for crude protein, ether extract and ash. The results also revealed that the highest record of DCP, crude fiber and TDNY was obtained by forage mixture of 75% E. clover: 25% ryegrass fertilized with Bio + O + N. Such higher yield of these characters hassecured a balanced ratio which is really needed for ruminants ration.

Hungaro Durum Rye—Breeding, Genome Composition and Amino Acid Content of the First Triticale Cultivar for Human Consumption

Hungaro triticale cultivar was bred for human consumption and animal fodder in Kisvárda, Hungary. The breeding method applied for the recombinant secondary hexaploid triticale proved to be effective since the favourable traits of wheat, durum wheat and rye were successfully combined in the genome of the new cultivar. The first aim was to characterize the genome composition. Multicolour genomic in situ hybridization (mcGISH) was used for the genome compositional analysis. According to the results, the cultivar contains the wheat AB genome (AABB = 28 chromosomes) and the whole rye (R) genome (RR = 14 chromosomes). The presence of D genome, however, could not be detected within the detection limit of the GISH method. Hungaro is the very first triticale in Hungary that has been used for human consumption (milling industry, baking industry, pasta production). In this study, amino acid content of flours was measured. Amino acid investigations on Hungaro durum rye’s flour showed that the quantity of essential amino acids, especially of methionine and cysteine, are higher in Hungaro durum rye than in the also investigated Ryefood rye cultivar and the GK ?thalom wheat cultivar. The genes inherited from the wheat species ensure high yield and great baking and dough making qualities. Therefore, its flour is suitable in itself for pastry and dough production (without mixing it with other flours). Its characteristics inherited from rye guarantee excellent abiotic and biotic resistance.

The American Quest for Identity： Theme and Form in the Catcher in the Rye, Invisible Man and Slaughterhouse Five

American society went through some especially speedy and drastic changes, after the World War II. Its transformation into a significant superpower as well as the horrors of the recent conflict is reflected in specific American novels and in the writing of certain elite novelists. They focus on human loss and a consequent search for identity and problematic belonging in a world of prejudice and hostility. This search for identity and aspiration to conformity with the new change is among of the main features of Salinger＇s the Catcher in the Rye, Ellison＇s Invisible Man and Vonnegut＇s Slaughterhouse Five. The paper focuses on certain similarities in theme and form which link these three novels and examines the extent of presentation of the style of narration set in motion by all three writers.

小说代词间接回指语汉译的伦理研究——以The Catcher in the Rye两个汉译本为例

本文以翻译伦理为观照,以The Catcher in the Rye的施咸荣、孙仲旭两个汉译本为例,探讨代词间接回指语的汉译。结果表明:(1)代词间接回指语的汉译方法从宏观上看,以直译为主,辅之以回指语的去除、明晰化和回指语的增益、转换等,虽然施译本的直译比例远超孙译本;当涉及到具体句子,不同译本中代词间接回指语的译法颇有差异;(2)"文化转向"背景下的翻译伦理及其再现、服务、交际、规范等伦理细则是一个合适的参照和分析手段,可以从微观上分析代词间接回指语各译例的得失;(3)两个汉译本服务于各自的时代,施译更多地想要忠实原文和作者,而孙译兼顾作者和读者。虽然总体上在回指语翻译方面孙译本超过施译本,但都体现了翻译伦理,因此各有千秋,说明了译者风格的客观存在。

A Study on the Interaction Between The Catcher in the Rye and the Readers From the Perspective of Iser’s Reader-Response

The Catcher in the Rye has been popular with readers since its publication,and critics at home and abroad have also conducted a lot of research on it.But they mainly proceed from the author and the text to evaluate and analyze,ignoring the readers who are essential participants in text interpretation in the eyes of reader-response critics.In order to make up for this shortcoming,this article will take Iser’s reader-response criticism as a research perspective,starting from the three aspects of implied reader,response-inviting structure,and gaps to explore the interaction between the text of The Catcher in the Rye and the readers during the reading process.This research is expected to provide a new interpretation of this novel and provide reference for future research in related fields.

An Analysis of the Tragedy in The Catcher in the Rye

Jerome David Salinger is a famous American novelist. His famous novel The Catcher in the Rye, vividly shows us imagination of a teenage after the Second World War. In this novel, the writer put contradiction between American teenager material life and the lack of spiritual life after the World War II, reflected on the protagonist Holden. In the novel, Amanda Louise Holden hated the adult hypocritical world and he wanted to escape from the reality, but his weakness decided that he finally ended in failure and the collapse of the spirit. Holden is a victim of the era and the tragedy of the times. This paper tries to make a detailed and deep analysis on the tragedy of Holden in the novel

Translation of The Catcher in the Rye from the Perspective of Skopos Theory——A Case Study of Sun Zhongxu's Translation Work

This paper analyzes the E-C translation of the book The Catcher in the Rye from the perspective of Skopos theory,taking Sun Zhongxu's translation work as an example,in which,literature method and case study method are adopted.The paper finds that Sun Zhongxu uses many colloquial expressions to convey the meaning of the source language.His translation strategies,his translation of American slangs,and his choices of words all reflect the three rules of Skopos theory.

Unveiling the distinctive traits of functional rye centromeres:minisatellites,retrotransposons,and R-loop formation

Centromeres play a vital role in cellular division by facilitating kinetochore assembly and spindle attachments.Despite their conserved functionality,centromeric DNA sequences exhibit rapid evolution,presenting diverse sizes and compositions across species.The functional significance of rye centromeric DNA sequences,particularly in centromere identity,remains unclear.In this study,we comprehensively characterized the sequence composition and organization of rye centromeres.Our findings revealed that these centromeres are primarily composed of long terminal repeat retrotransposons(LTR-RTs)and interspersed minisatellites.We systematically classified LTR-RTs into five categories,highlighting the prevalence of younger CRS1,CRS2,and CRS3 of CRSs(centromeric retrotransposons of Secale cereale)were primarily located in the core centromeres and exhibited a higher association with CENH3 nucleosomes.The minisatellites,mainly derived from retrotransposons,along with CRSs,played a pivotal role in establishing functional centromeres in rye.Additionally,we observed the formation of R-loops at specific regions of CRS1,CRS2,and CRS3,with both rye pericentromeres and centromeres exhibiting enrichment in R-loops.Notably,these R-loops selectively formed at binding regions of the CENH3 nucleosome in rye centromeres,suggesting a potential role in mediating the precise loading of CENH3 to centromeres and contributing to centromere specification.Our work provides insights into the DNA sequence composition,distribution,and potential function of R-loops in rye centromeres.This knowledge contributes valuable information to understanding the genetics and epigenetics of rye centromeres,offering implications for the development of synthetic centromeres in future plant modifications and beyond.

重金属-PAHs污染土壤植物微生物修复效果及机理

针对焦化厂等土壤中铬(Cr)、砷(As)重(类)金属和菲(Phe)、苯并[a]芘(BaP)等多环芳烃(PAHs)风险高、绿色低碳修复技术缺乏等突出问题,以黑麦草-玉米-食醚红球菌(Rhodococcus aetherivorans,BW2)开展植物微生物联合。结果表明,63 d后植物微生物联合修复组土壤中Cr、As、Phe和BaP浓度分别下降9.63%、5.28%、45.14%和26.87%,其中重金属的去除主要依赖于植物作用,而PAHs去除则在很大程度上取决于微生物与植物的共同作用;植物主要吸收可提取态重金属,63 d后不同修复组土壤中可交换态Cr平均下降2.19%,而As则由于降解作用使修复过程中可交换态增加;可交换态Cr和碳酸盐结合态Cr与土壤中放线菌门呈强正相关,2种形态As则与拟杆菌门、装甲菌门、FBP菌门和浮霉菌门呈正相关。污染物去除效率间存在明显的相互作用,同时受酶活性和不同功能微生物丰度的动态影响,反之土壤微生物群落结构和功能也在土壤污染物种类、浓度及理化性质等综合作用下发生改变。