基于Searle Center Database,用生存分析方法分析SEP免费政策对SSO成员生存率的影响。研究表明在ICT行业中,SEP免费政策反而会提高成员的生存率,当成员同时加入多个SSO或在其他SSO中声明SEP时,这种效应尤其明显。认为在复杂技术行业中,...基于Searle Center Database,用生存分析方法分析SEP免费政策对SSO成员生存率的影响。研究表明在ICT行业中,SEP免费政策反而会提高成员的生存率,当成员同时加入多个SSO或在其他SSO中声明SEP时,这种效应尤其明显。认为在复杂技术行业中,技术供给企业可能存在着迂回获利模式,SSO可以改进政策制定思路避免陷入"标准推广or标准质量"的两难选择。展开更多
A cw operation and a passively Q-switched （PQS） Ho：SSO laser （Cr2＋ ：ZnSe as a saturable absorber） end-pumped by a TIn：YAP laser operating at near room temperature are reported. It is the first time to report a...A cw operation and a passively Q-switched （PQS） Ho：SSO laser （Cr2＋ ：ZnSe as a saturable absorber） end-pumped by a TIn：YAP laser operating at near room temperature are reported. It is the first time to report a PQS Ho：SSO laser. For the ew mode, a maximum cw output power of 3.0 W is obtMned, corresponding to a slope efficiency of 31.4%. For the PQS mode, a Cr2＋ ：ZnSe is used as the saturable absorber, with transmission of 88.4% at 2112nm. A maximum pulse energy of 1.29mJ is obtained, corresponding to the pulse repetition frequency of 2.42kHz. In this study, we change the distance between Cr2＋ ：ZnSe and the output mirror to research the pulse characteristic of the PQ, S Ho：SSO laser. The minimum pulse width of 73.5ns is obtained, corresponding to the pulse energy of O. 9 mJ and the pulse repetition frequency of 2.65 kHz.展开更多
HLA-SSO基因分型采用序列特异性寡核苷酸（sequence specific oligonucleotide,SSO）引物来鉴定PCR扩增产物中的等位基因。该方法基于标记的单链PCR产物与SSO探针进行杂交,通过PCR扩增,获得单链及双链混合PCR产物,用于扩增的引物标记有...HLA-SSO基因分型采用序列特异性寡核苷酸（sequence specific oligonucleotide,SSO）引物来鉴定PCR扩增产物中的等位基因。该方法基于标记的单链PCR产物与SSO探针进行杂交,通过PCR扩增,获得单链及双链混合PCR产物,用于扩增的引物标记有生物素,因此扩增所得的PCR产物均被生物素标记。双链PCR产物经过变性解链成单链,所有单链与微珠上的SSO探针特异性结合,然后用荧光标记,之后与生物素结合,利用Luminex平台进行HLA-Ⅰ类和HLA-Ⅱ类等位基因的DNA分型。展开更多
文摘基于Searle Center Database,用生存分析方法分析SEP免费政策对SSO成员生存率的影响。研究表明在ICT行业中,SEP免费政策反而会提高成员的生存率,当成员同时加入多个SSO或在其他SSO中声明SEP时,这种效应尤其明显。认为在复杂技术行业中,技术供给企业可能存在着迂回获利模式,SSO可以改进政策制定思路避免陷入"标准推广or标准质量"的两难选择。
基金Supported by the National Natural Science Foundation of China under Grant No 61405046the Natural Science Foundation of Heilongjiang Province under Grant No 51305089
文摘A cw operation and a passively Q-switched （PQS） Ho：SSO laser （Cr2＋ ：ZnSe as a saturable absorber） end-pumped by a TIn：YAP laser operating at near room temperature are reported. It is the first time to report a PQS Ho：SSO laser. For the ew mode, a maximum cw output power of 3.0 W is obtMned, corresponding to a slope efficiency of 31.4%. For the PQS mode, a Cr2＋ ：ZnSe is used as the saturable absorber, with transmission of 88.4% at 2112nm. A maximum pulse energy of 1.29mJ is obtained, corresponding to the pulse repetition frequency of 2.42kHz. In this study, we change the distance between Cr2＋ ：ZnSe and the output mirror to research the pulse characteristic of the PQ, S Ho：SSO laser. The minimum pulse width of 73.5ns is obtained, corresponding to the pulse energy of O. 9 mJ and the pulse repetition frequency of 2.65 kHz.
文摘HLA-SSO基因分型采用序列特异性寡核苷酸（sequence specific oligonucleotide,SSO）引物来鉴定PCR扩增产物中的等位基因。该方法基于标记的单链PCR产物与SSO探针进行杂交,通过PCR扩增,获得单链及双链混合PCR产物,用于扩增的引物标记有生物素,因此扩增所得的PCR产物均被生物素标记。双链PCR产物经过变性解链成单链,所有单链与微珠上的SSO探针特异性结合,然后用荧光标记,之后与生物素结合,利用Luminex平台进行HLA-Ⅰ类和HLA-Ⅱ类等位基因的DNA分型。
