Effects of phosphorus limitation on sinking velocities of phytoplankton during summer in the Changjiang River Estuary

The sinking of phytoplankton is critical to organic matter transportation in the ocean and it is an essential process for the formation of coastal hypoxic zones.This study was based on a field investigation conducted during the summer of 2022 in the Changjiang River(Yangtze River) Estuary(CJE) and its adjacent waters.The settling column method was employed to measure the sinking velocity(SV) of different size fractions of phytoplankton at the surface of the sea and to analyze their environmental control mechanisms.The findings reveal significant spatial variation in phytoplankton SV(-0.55-2.41 m/d) within the CJE.High-speed sinking was predominantly observed in phosphate-depleted regions beyond the CJE front.At the same time,an upward trend was more commonly observed in the phosphate-rich regions near the CJE mouth.The SV ranges for different sizefractionated phytoplankton,including micro-(>20 μm),nano-(2-20 μm),and picophytoplankton(0.7-2 μm),were-0.50-4.74 m/d,-1.04-1.59 m/d,and-1.24-1.65 m/d,respectively.Correlation analysis revealed a significant negative correlation between SV and dissolved inorganic phosphorus(DIP),implying that the influence of DIP contributes to SV.The variations in phytoplankton alkaline phosphatase activity suggested a significant increase in SV across all size fractions in the event of phosphorus limitation.Phytoplankton communities with limited photo synthetic capacity(maximum photochemical efficience,Fv/Fm <0.3) were found to have higher SV than that of communities with strong capacity,suggesting a link between sinking and alterations in physiological conditions due to phosphate depletion.The findings from the in situ phosphate enrichment experiments confirmed a marked decrease in SV following phosphate supplementation.These findings suggest that phosphorus limitation is the primary driver of elevated SV in the CJE.This study enhances the comprehension of the potential mechanisms underlying hypoxic zone formation in the CJE,providing novel insights into how nearshore eutrophication influences organic carbon migration.

Effects of nutrient limitations on the sinking velocity of Thalassiosira weissflogii

The sinking of diatoms is critic al to the formation of oceanic biological pumps and coastal hypoxic zones.However,little is known about the effects of different nutrient restrictions on diatom sinking.In this study,we measured the sinking velocity(SV) of Thalassiosira weissflogii using a new phytoplankton video observation instrument and analyzed major biochemical components under varying nutrient conditions.Our results showed that the SV of T.weissflogii under different nutrient limitation conditions varied substantially.The highest SV of(1.77±0.02) m/d was obtained under nitrate limitation,signific antly surpassing that under phosphate limitation at(0.98±0.13) m/d.As the nutrient limitation was released,the SV steadily decreased to(0.32±0.03) m/d and(0.15±0.05) m/d,respectively.Notably;under conditions with limited nitrate and phosphate concentrations,the SV values of T.weissflogii significantly positively correlated with the lipid content(P <0.001),with R^(2) values of 0.86 and 0.69,respectively.The change of the phytoplankton SV was primarily related to the intracellular compo sition,which is controlled by nutrient conditions but did not significantly correlate with transparent extracellular polymer and biosilica contents.The results of this study help to understand the regulation of the vertical sinking process of diatoms by nutrient restriction and provide new insights into phytoplankton dynamics and their relationship with the marine nutrient structure.

Application of Numerical Simulation for Analysis of Sinking Characteristics of Purse Seine

This study applies the mass-spring system to model the dynamic behavior of a submerged net panel similar to the shooting process in actual purse seine fishing operation. Modeling indicates that there is insufficient stretching with the net panel under the floatline in the prophase of the shooting process. Sinkers at different locations along the leadline descend successively after submergence, and the sinking speed decreases gradually with elapsed time until attainment of a stable state. Designs with different current speeds and sinker weights are executed to determine the dimensional shape and sinking characteristics of the net. The net rigged with greater sinker weight gains significantly greater sinking depth without water flow. Compared with the vertical spread of the net wall in static water, the middle part of the netting presents a larger displacement along the direction of current under flow condition. It follows that considerable deformation of the netting occurs with higher current speed as the sinkers affected by hydrodynamic force drift in the direction of current. The numerical model is verified by a comparison between simulated results and sea measurements. The calculated values generally coincide with the observed ones, with the former being slightly higher than the latter. This study provides an implicit algorithm which saves computational loads for enormous systems such as purse seines, and ensures the accuracy and stability of numerical solutions in a repetitious iteration process.

The inner circulation mechanism in sinking column formation in North China coal field ——A case study in Fengfeng mining areas

Sinking columns are one of the most hazardous geological structures in north Chine typed coal fields. To understand the sinking column formation, to judge if the columns are hazardous or not, the inner circle mechanism of groundwater in forming sinking columns in the relatively isolated areas was proposed according to the investigation on formation or distribution of sinking columns in some north China typed coal fields, such as Fengfeng, Xiangtai, Huaibei, Yangquan and so on. The heat generated by magma energized groundwater circulation and developed limestone karst in the relatively isolated areas. The CaCO3 solution accelerated by geothermal abnormality, the effect of high content of CO2 on dissolution and saturation of CaCO3 were discussed. Compared the forming conditions of the sinking columns developed in other coal mining areas in north China coal field, the paper deduced that the above factors collude karst development and sinking column formation, the columns in current geothermal areas are permeable and those in high coal rank areas or in paleo abnormally geothermal areas. The paper suggested that the sinking columns in the current abnormally geothermal areas were permeable, but those in the areas with high coal rank were not on contract.

Concentrations and sinking rates of transparent exopolymer particles(TEPs)in a coastal sea:the Changjiang River(Yangtze River)Estuary

Transparent exopolymer particles(TEPs)are ubiquitous throughout the oceans,and their sedimentation is considered an efficient biological carbon sink pathway.To investigate the role of coastal TEPs in sinking carbon from the upper layer,samples were collected in the spring and summer of 2011 in the Changjiang River(Yangtze River)Estuary,a typical coastal water.The concentrations and sinking rates of TEPs were measured,and potential sedimentation flux of TEPs was estimated.TEPs concentrations ranged from 40.00μg/L to 1040.00μg/L(mean=(209.70±240.93)μg/L)in spring and 56.67μg/L to 1423.33μg/L(mean=(433.33±393.02)μg/L)in summer,and they were higher at bloom stations than at non-bloom stations during both cruises.A significant positive correlation between TEPs concentration and chlorophyll a(Chl a)concentration was detected,suggesting that phytoplankton was the primary source of TEPs in this area.TEPs sinking rates ranged from 0.08 m/d to 0.57 m/d with a mean of(0.28±0.14)m/d in spring and 0.10 m/d to 1.08 m/d with a mean of(0.34±0.31)m/d in summer.The potential sedimentation flux of TEP-C ranged from 4.95 mg/(m2·d)to 29.40 mg/(m2·d)with a mean of(14.66±8.83)mg/(m2·d)in spring and 6.80 mg/(m2·d)to 30.45 mg/(m2·d)with a mean of(15.71±8.73)mg/(m2·d)in summer,which was^17.81%to 138.27%(mean=65.15%±31.75%)of sedimentation flux of phytoplankton cells in the study area.Due to the increase of TEPs concentrations and their sinking rates,sedimentation fluxes of TEPs at the bloom station were obviously higher than at the non-bloom station during both cruises.This study indicates that TEPs serve as a carbon sink in the Changjiang River Estuary,especially during bloom events,and their sedimentation should be taken into account when we study the carbon sedimentation in the coastal sea.

Influence of light availability on the specific density,size and sinking loss of Anabaena flos-aquae and Scenedesmus obliquus

Harmful algal blooms in eutrophic waters pose a serious threat to freshwater ecosystems and human health. In-situ light availability control is one of the most commonly used technologies to suppress algae in lakes and reservoirs. To develop a better understanding of the effects of light on algal growth, specific density, colony size and sinking loss, Anabaena flos-aquae(cyanobacteria) and Scenedesmus obliquus(green algae) were evaluated in varying light scenarios. The results showed that the specific density and colony size of these two species varied during growth, and there were obvious differences among the light scenarios. At the end of exponential phase, S. obliquus incubated under light-limited condition maintained a higher specific density and formed larger aggregates, whereas A. flos-aquae formed a longer filament length. Both species exhibited higher sinking loss rates with lower light availability. These results implied that the sinking loss rate was not always constant but should be considered as a variable response to the change of light availability, and in-situ light availability control might result in a significant increase of the sinking loss of algae due to the change of size and specific density, thereby further affecting the algal biomass in the water column.

Coupled thermo-hydro-mechanical modeling of frost heave and water migration during artificial freezing of soils for mineshaft sinking

Artificial freezing of water-bearing soil layers composing a sedimentary deposit can induce frost heave and water migration that affect the natural stress-strain state of the soil layers and freezing process.In the present paper,a thermo-hydro-mechanical(THM)model for freezing of water-saturated soil is proposed to study the effects of frost heave and water migration in frozen soils on the formation of a frozen wall and subsequent excavation activity for sinking a vertical shaft.The governing equations of the model are formulated relative to porosity,temperature,and displacement which are considered as primary variables.The relationship between temperature,pore water,and ice pressure in frozen soil is established by the Clausius-Clapeyron equation,whereas the interaction between the stress-strain behavior and changes in porosity and pore pressure is described with the poromechanics theory.Moreover,constitutive relations for additional mechanical deformation are incorporated to describe volumetric expansion of soil during freezing as well as creep strain of soil in the frozen state.The ability of the proposed model to capture the frost heave of frozen soil is demonstrated by a comparison between numerical results and experimental data given by a one-sided freezing test.Also to validate the model in other freezing conditions,a radial freezing experiment is performed.After the validation procedure,the model is applied to numerical simulation of artificial freezing of silt and sand layers for shaft sinking at Petrikov potash mine in Belarus.Comparison of calculated temperature with thermal monitoring data during active freezing stage is presented.Numerical analysis of deformation of unsupported sidewall of a shaft inside the frozen wall is conducted to account for the change in natural stress-strain state of soil layers induced by artificial freezing.

Temporal variation in biodeposit organic content and sinking velocity in long-line shellfish culture

We measured the organic content and sinking velocities of biodeposits from two scallop species(Chlamys farreri,Patinopecten yessoensis) and abalone(Haliotis discus hannai) that were cultured on suspended long-lines.Measurements were conducted every two months from April 2010 to February 2011.The shellfish were divided into three size groups(small,middle,and big sizes).At each sample point,we assessedbiodeposit organic content,average sinking velocity,the frequency distribution of sinkingvelocities,and the correlation between organic content and sinking velocity.The organic content of biodeposits varied significantly among months(P<0.05) and the pattern of change varied among species.Sinking velocities varied significantly,ranging from <0.5 cm/s to >1.9 cm/s.The sinking velocities of biodeposits from C.farreri and P.yessoensis were 0.5-1.5 cm/s and from H.discus hannai were <0.7 cm/s.The organic content was significantly negatively correlated to the sinking velocity of biodeposits in C.farreri(P<0.001) and P.yessoensis(P<0.05).

The Effect of the Sinking of Isotope Grains in Water Injection Profile Logging

In the process of oil field exploitation, radioactive isotope tracer water injection profile logging is a main method to determine the water-intake capacity of the target intervals. For those general water injection wells, whose perforated intervals are beyond the tubing shoe, the sinking speed of isotope grains is related to the density and salinity of the injection water and the tubing dimension. We studied the motion of the isotope grains in the water injection wells by both theoretical calculation and field experiment. The result shows that if the water injection quantity is over 20 m＆3/d, and the density of the isotope grains, whose diameter is 100-600 μm, is 1.06 g/cm^3, then the logging technique for this kind of water injection wells will be appropriate. Whether water injection profile logging is successful in the well, whose perforated intervals are beyond the tubing shoe, depends on the sinking speed of the tracer grains.

ECCENTRICITY CHANGE IN TUBE SINKING

The rectification of eccentricity was revealed to be more effective in tube sinking than drawing with a mandrel.The ratio of thickness to diameter of tube blank on both sides of thick and thin wall seems to be the principal influential factor in rectifying eccentricity.The greater the ini- tial outside diameter and the initial wall thickness difference are and the less the initial wall thickness of the tube blank is,the better the rectification is.

Analysis of the rising and sinking movement of the crust in Qinghai－Xizang Plateau，China

The average swelling height of Qinghai-Xizang Plateau(Tibet) is about 4.1 km.The area is about 2 300 000 km2.It is the highest and the largest plateau in the world.Mount Qomolangma,the highest peak in the world,is located in the southern fringe of the plateau,and its height is still increasing.What is the driving force for the rising of the Qinghai-Xizang plateau?How high will the plateau still rise from now on? These questions are much concerned by people.In this paper the distribution of the pressure at different depths in the region of Qinghai-Xizang Plateau are derived according to the three-dimensional structures of the S-wave velocity in the crust and upper mantle.The crust and upper mantle structures of the Qinghai-Xizang Plateau is deliberated on the basis of the distribution with a comprehensive analysis on the rock types,earthquakes and the relative crust movements.Then the two questions raised above are discussed.

Rapid prediction of floating and sinking components of raw coal

A model that rapidly predicts the density components of raw coal is described.It is based on a threegrade fast float/sink test.The recent comprehensive monthly floating and sinking data are used for comparison.The predicted data are used to draw washability curves and to provide a rapid evaluation of the effect from heavy medium induced separation.Thirty-one production shifts worth of fast float/sink data and the corresponding quick ash data are used to verify the model.The results show a small error with an arithmetic average of 0.53 and an absolute average error of 1.50.This indicates that this model has high precision.The theoretical yield from the washability curves is 76.47% for the monthly comprehensive data and 81.31% using the model data.This is for a desired cleaned coal ash of 9%.The relative error between these two is 6.33%,which is small and indicates that the predicted data can be used to rapidly evaluate the separation effect of gravity separation equipment.

Research on the Development Status of Community Group Buying in Sinking Market

Community group buying is a brand-new retail model,and this retail model is not limited to the first-and second-tier cities in China,and has gradually penetrated into the sinking market.The sinking consumer market is changing with each passing day,and community group buying will surely usher in more help in the promotion and application of sinking market areas.The network platform of community group buying also gives full play to the resource advantages of"internet plus",expands the sales of fresh agricultural products,and constantly improves logistics problems to meet the consumer demand of sinking market.This paper introduces the development status of community group buying in China's sinking market,analyzes the existing problems and puts forward corresponding suggestions,and then looks forward to the future development of the industry.Finally,the future development of community group buying platform in the sinking market is prospected.

Establishing Submersed Macrophytes via Sinking and Colonization of Shoot Fragments Clipped off Manually

In this paper, sinking and growth of apexes and mid-stems of Myriophyllum spicatum L., Hydrilla verticillata （L.f.） Royle and Ceratophyllum demersum L. in concrete ponds containing eutrophic water and sediment were investigated. Sinking rates of apexes and mid-stems reached 34.8% and 4.4% at the 6 th day and 91.1% and 66.7% at the 22 nd day for M. spicatum, 57.8% and 55.6% at the 6 th day and 100% and 97.8% at the 22 nd day for H. vertieillata, 18.9% and 86.7% at the 6 th day and 95.6% and 100% at the 22 nd day for C. demersum, respectively. Most sunken fragments established themselves successfully with significant growth. Total shoot length ofplantlets developed from apexes and mid-stems increased by 399% and 61% for M. spicatum, 593% and 256% for H. vertieillata and 114% and 104% for C. demersum, respectively. The results showed that it was feasible to establish submersed macrophytes via sinking and colonization of shoot fragments clipped off manually.

Evolution of inner wall wrinkle defects in the sinking zone of a thick-walled steel tube during radial forging

An axial wrinkle defect was observed in the inner wall of the sinking zone of a thick-wall steel tube processed by cold radial forging.Wrinkles can evolve into fissures.The present study focuses on the evolution of wrinkles and the effects of process parameters on them using a three-dimensional radial forging process finite element model,radial forging experiments,and surface morphology observations.The results indicated that the vertical section angle of the hammer die and the size of the tube blank significantly affect the evolution of wrinkles.The height-to-width ratioλwas introduced to describe the morphology of wrinkles.It had an approximately linear relationship with the radius reduction in the sinking zone.There was a linear correlation between the growth slope ofλand the axial to circumferential strain ratio|εr/εθ|,which can predict theλunder few process parameters.For the 30SiMn2MoVA steel,at the junction of the forging and sinking zones,the threshold ofλof the wrinkle that can evolve into a fissure is approximately 1.123.

基于局部密度聚类的WSN多Sink节点部署研究

针对无线传感器网络中传感器节点能量受限,网络生命周期短的问题,在考虑网络成本的情况下,提出一种基于节点局部密度聚类的多Sink节点优化部署算法。首先,基于多属性因子构建聚类决策函数确定Sink节点部署位置,完成传感器节点聚类;然后,根据下一跳节点与Sink节点间距离最短准则搜索并形成数据传输路径;最后,以网络生命周期成本比最大化为依据确定最优的Sink节点数目,实现多Sink节点优化部署。仿真结果表明:与已有算法相比,所提算法能够有效延长网络生命周期,具有较高的网络生命周期能效比。

跳数受限下的传感云网络多Sink节点重选址方法

当传感云网络中的Sink节点处于静止状态时,分布在其周围的邻居节点极易出现“能量空洞”,导致能量消耗不均,节点出现不同的网络生存期,影响节点间数据正常传输。为此,在限制节点跳数的前提下,提出一种多Sink节点重选址方法。基于Sink节点寿命和传感云网络代价函数引入质心理论,建立质点系并确定质心位置;将Sink节点向流量大的节点移动,并与其他Sink节点相互协作、避免发生冲突,直至到达质心位置,实现多Sink节点重选址。结果表明,所提方法重选址的节点生存时间最高为1 000 s为,数据包交付率为98%,节点平均剩余能量值高于0.6 J。

Evaluation of excavation damaged zones(EDZs)in Horonobe Underground Research Laboratory(URL)

Excavation of underground caverns,such as mountain tunnels and energy-storage caverns,may cause the damages to the surrounding rock as a result of the stress redistribution.In this influenced zone,new cracks and discontinuities are created or propagate in the rock mass.Therefore,it is effective to measure and evaluate the acoustic emission(AE)events generated by the rocks,which is a small elastic vibration,and permeability change.The authors have developed a long-term measurement device that incorporates an optical AE(O-AE)sensor,an optical pore pressure sensor,and an optical temperature sensor in a single multi-optical measurement probe(MOP).Japan Atomic Energy Agency has been conducting R&D activities to enhance the reliability of high-level radioactive waste(HLW)deep geological disposal technology.In a high-level radioactive disposal project,one of the challenges is the development of methods for long-term monitoring of rock mass behavior.Therefore,in January 2014,the long-term measurements of the hydro-mechanical behavior of the rock mass were launched using the developed MOP in the vicinity of 350 m below the surface at the Horonobe Underground Research Center.The measurement results show that AEs occur frequently up to 1.5 m from the wall during excavation.In addition,hydraulic conductivity increased by 2e4 orders of magnitude.Elastoplastic analysis revealed that the hydraulic behavior of the rock mass affected the pore pressure fluctuations and caused micro-fractures.Based on this,a conceptual model is developed to represent the excavation damaged zone(EDZ),which contributes to the safe geological disposal of radioactive waste.

Dynamics and vibration reduction performance of asymmetric tristable nonlinear energy sink

With its complex nonlinear dynamic behavior,the tristable system has shown excellent performance in areas such as energy harvesting and vibration suppression,and has attracted a lot of attention.In this paper,an asymmetric tristable design is proposed to improve the vibration suppression efficiency of nonlinear energy sinks(NESs)for the first time.The proposed asymmetric tristable NES(ATNES)is composed of a pair of oblique springs and a vertical spring.Then,the three stable states,symmetric and asymmetric,can be achieved by the adjustment of the distance and stiffness asymmetry of the oblique springs.The governing equations of a linear oscillator(LO)coupled with the ATNES are derived.The approximate analytical solution to the coupled system is obtained by the harmonic balance method(HBM)and verified numerically.The vibration suppression efficiency of three types of ATNES is compared.The results show that the asymmetric design can improve the efficiency of vibration reduction through comparing the chaotic motion of the NES oscillator between asymmetric steady states.In addition,compared with the symmetrical tristable NES(TNES),the ATNES can effectively control smaller structural vibrations.In other words,the ATNES can effectively solve the threshold problem of TNES failure to weak excitation.Therefore,this paper reveals the vibration reduction mechanism of the ATNES,and provides a pathway to expand the effective excitation amplitude range of the NES.

Organic geochemical studies of sinking particulate material in China sea area(I)──Organic matter fluxes and distributional features of hydrocarbon compounds and fatty acids

Sinking particulate material collected from Nansha Yongshu reef lagoon and the continental shelf of the East China Sea by sediment traps has been analyzed and studied for the first time using organic geochemical method. The results show that about half of the sinking particulate organic matter in the two study areas are consumed before reaching the depth of 5 m to the sea floor and the degree of this consumption in Yongshu reef lagoon is larger than that in the continental shelf of the East China Sea. The distributions of hydrocarbons and fatty acids indicate that the minor difference of biological sources of sinking particulate organic matter exists between Yongshu reef lagoon and the continental shelf of the East China Sea, but they mainly come from marine plankton. Stronger biological and biochemical transformations of sinking particulate organic matter are also observed and the intensity of this transformation in Yongshu reef lagoon is greater than that in the continental shelf of the East China Sea. It is found that the occurrence of C 25 highly branched isoprenoid (HBI) diene may be related to the composition of diatom species.