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Tissue distribution of cadmium and its effect on reproduction in Spodoptera exigua
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作者 Honghua Su Menglu Wu +3 位作者 Yong Yang Yan Deng Yizhong Yang Qingming Sun 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期195-204,共10页
Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua... Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua exposed to cadmium and its effects on the growth and development of the parents and the offspring were investigated.Under the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of S.exigua increased in a dose-dependent manner.At the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat body.In addition,the cadmium content in ovaries was much higher than in testes.When F1S.exigua was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding F1generation.Even in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental generation.The fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure levels.When only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)treatments.The results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution. 展开更多
关键词 heavy metal pollution CADMIUM spodoptera exigua tissue distribution REPRODUCTION
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Induced CTL-S15 gene expression by Bacillus thuringiensis declines susceptibility in Spodoptera exigua
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作者 Jianqiang Bao Yuxuan Chen +6 位作者 Suwan Jiang Rui Liu Xi Zhang Fangzheng Zhang Zhiwei Chen Chen Luo Hailong Kong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第9期3078-3088,共11页
It has been reported that C-type lectins(CTLs),which are pattern recognition receptors of the insect innate immunity response,may compete with Cry toxin for the receptor alkaline phosphatase to decrease its toxicity i... It has been reported that C-type lectins(CTLs),which are pattern recognition receptors of the insect innate immunity response,may compete with Cry toxin for the receptor alkaline phosphatase to decrease its toxicity in insects.However,to date,which CTLs affect larval susceptibility to Bt in Spodoptera exigua is not clear.In this study,33 CTL genes were identified from S.exigua.Based on the number of carbohydrate-recognition domains(CRDs)and the domain architectures,they were classified into three groups:(1)nineteen CTL-S(single-CRD),(2)eight immulectin(dual-CRD)and(3)six CTL-X(CRD with other domains).RT-qPCR analysis revealed that expression levels of SeCTL-S15,IML-4 and CTL-X6 were upregulated after challenge with Bt and Cry1Ab.Tissue and developmental stage expression analysis showed that only SeCTL-S15 was mainly expressed in the midgut and larva,respectively.Knockdown of SeCTL-S15 significantly increased Bt susceptibility,as indicated by reduced survival and larval weight.These results suggest that CTL-S15 might play a vital role in the low susceptibility of larvae to Bt in S.exigua.Our results provide new insights into CTL function in insects. 展开更多
关键词 spodoptera exigua Bacillus thuringiensis SUSCEPTIBILITY C-type lectins
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Insect Resistance of Different Tissues of Transgenic Cotton to Spodoptera exigua(Hbner) 被引量:10
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作者 苏宏华 李达 +1 位作者 范娜 杨益众 《Agricultural Science & Technology》 CAS 2010年第8期140-144,共5页
[Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua (Hbner). [Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK1... [Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua (Hbner). [Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK12 and SGK321 were used to feed S. exigua neonates respectively. Survival larvae and dead ones were counted on the 3rd,the 7th,the 10th,the 16th and the 19th day; meanwhile,the pupae amount was recorded,and the pupae weight was measured at the 24th h after pupation. [Result] The survival curves,pupation rates and pupae weights of S. exigua feeding on different tissues of transgenic cotton were not significantly different from those of S. exigua feeding on the corresponding tissues of conventional cotton; pupation rate of S. exigua feeding on different leaves of the same cotton variety were not significantly different from each other,but all higher than that of S. exigua feeding on the flowers of that cotton; and there were no differences among pupation weights of S. exigua feeding on different leaves or flowers of the same cotton variety. [Conclusion] Transgenic cotton showed weak resistance to S. exigua. Hence,in the transgenic cotton fields,more attention should be paid to occurrence trend of S. exigua and its control. 展开更多
关键词 Transgenic cotton spodoptera exigua Different tissues of cotton Insect resistance
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Field Trials of 20% Tebufenozide SC against Spodoptera exigua Hbner
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作者 杨彬 姚安平 周小毛 《Plant Diseases and Pests》 CAS 2010年第1期50-51,62,共3页
[Objective] The study was aimed to determine and evaluate control effect and security of 20% tebufenozide SC against Spodoptera exigua HObner, which would provide technical support for its promotion and utilization. [... [Objective] The study was aimed to determine and evaluate control effect and security of 20% tebufenozide SC against Spodoptera exigua HObner, which would provide technical support for its promotion and utilization. [ Method] By using foliar spray method, control effects of different concentration of 20% tebufenozide SC and 25 g/L deltamethrin EC against S. exigua were determined. [ Result] 20% tebufenozide SC had better control effects on S. exigua. After spraying for 3 and 7 d, control effects of 20% tebufenozide SC in each treatment could reach 81% and 84%, which was higher than 25 g/L deltamethdn EC treatment. [ Conclusion] 20% tebufenozide SC for controlling S. exigua had good effect, which had permanent effectiveness for over 7 d. It also caused less environmental pollution, and was safe to cabbage, being the ideal pesticide for controlling S. exigua in vegetables. 展开更多
关键词 TEBUFENOZIDE SC spodoptera exigua HObner Control effect
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交配和温度对甜菜夜蛾(Spodoptera exigua)雌蛾性信息素产生的影响 被引量:33
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作者 董双林 杜家纬 《应用生态学报》 CAS CSCD 2002年第12期1633-1636,共4页
研究表明 ,交配对甜菜夜蛾雌蛾性信息素各组分的滴度、比例及其在个体间的变异均无显著的影响 ,因此交配不影响雌蛾在下一暗期的再次求偶和交配 .环境温度对甜菜夜蛾性信息素的产生则有显著的影响 .15~ 2 5℃最有利于甜菜夜蛾性信息素... 研究表明 ,交配对甜菜夜蛾雌蛾性信息素各组分的滴度、比例及其在个体间的变异均无显著的影响 ,因此交配不影响雌蛾在下一暗期的再次求偶和交配 .环境温度对甜菜夜蛾性信息素的产生则有显著的影响 .15~ 2 5℃最有利于甜菜夜蛾性信息素的产生 ,而 10℃低温和 35℃高温则使性信息素产生受到明显影响 ,其中以 10℃低温的影响更甚 .10℃低温处理不仅使各组分的滴度产生显著改变 ,而且使二元性信息素组分的比例受到显著影响 ,并使二元组分通讯信道的个体间变异明显增大 (CV >10 % ) .这些改变必将扰乱甜菜夜蛾雌雄间的正常交配 . 展开更多
关键词 交配 温度 甜菜夜蛾 雌蛾 性信息素产生 影响
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Effect of Host Plants on Development,Fecundity and Enzyme Activity of Spodoptera exigua (Hübner)(Lepidoptera:Noctuidae) 被引量:11
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作者 ZHANG Bin LIU Huai +1 位作者 Helen Hull-Sanders WANG Jin-jun 《Agricultural Sciences in China》 CAS CSCD 2011年第8期1232-1240,共9页
The beet armyworm,Spodoptera exigua (Lepidoptera:Noctuidae),is an economically important pest of crops worldwide,attacking plants from over 20 families including trans-continental agricultural cotton,corn and citru... The beet armyworm,Spodoptera exigua (Lepidoptera:Noctuidae),is an economically important pest of crops worldwide,attacking plants from over 20 families including trans-continental agricultural cotton,corn and citrus crops.In this study,performance and subsequent enzyme activity of beet armyworm reared on host plants from five families were investigated.Significant differences were found in development,fecundity and enzyme activity on different host plants.Survival rate was the highest (42.8%) on asparagus lettuce (Lactuca sativar var.asparagina) and the lowest (17.0%) on sweet pepper (Capsicum annuum).Larval duration was the shortest on asparagus lettuce (12.0 d),and was 43.4% longer on sweet peppers (21.2 d).The activity of acetylcholine esterase (AChE) and carboxylesterase (CarE) in 3rd instar larvae,and soluble carbohydrate and crude protein contents in different host plants were determined.AChE activity was the highest in the larvae feeding on Chinese cabbage (Brassica rapa),but declined by nearly 60% on maize (Zea mays) seedlings.The ratio of soluble carbohydrate content to crude protein content in host plants was found to have a positive effect on oviposition and a negative correlation with larval duration and life time (from larval to adult stages) of the insect. 展开更多
关键词 beet armyworm spodoptera exigua host plants NUTRITION enzyme activity
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A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua(Lepidoptera:Noctuidae) 被引量:10
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作者 LU Qiong ZHANG Yongojun +6 位作者 CAO Guang-chun ZHANG Li-li LIANG Ge-mei LU Yan-hui WU Kong-ming GAO Xi-wu GUO Yu-yuan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第4期628-638,共11页
Cry toxins produced by Bacillus thuringiensis(Bt) are effective biological insecticides against certain insect species.In this study,bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua.We... Cry toxins produced by Bacillus thuringiensis(Bt) are effective biological insecticides against certain insect species.In this study,bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua.We also identified a cadherin-like gene in S.exigua that could enhance the toxicity of Cry1B and Cry1C.The cadherin-like gene identified from the larvae midgut tissue was cloned by reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE).The full-length cDNA of the gene consisted of 5 220 bp encoding 1 740 amino acid with a predicted molecular mass of 196 kD.BLAST search analysis showed that the predicted amino acid sequence had a high sequence identity to the published sequences of cadherin-like proteins from other Lepidoptera insects.Spatial expression of the cadherin-like gene detected by qRT-PCR analysis revealed that the cadherin-like gene was mainly present in the gut of 4th instar larvae and during different life stages.The results suggested that the commercial development of this synergist has the potential to enhance Cry1B and Cry1C toxicity against Lepidoptera insects. 展开更多
关键词 Bacillus thuringiensis cadherin-like spodoptera exigua enhancement toxicity
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甜菜夜蛾(Spodoptera exigua)自然种群与抗拟除虫菊酯有关的L1014F突变的发现 被引量:1
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作者 纠敏 安世恒 +3 位作者 张国彦 张蕾 郭线茹 马继盛 《河南农业大学学报》 CAS CSCD 2002年第2期106-110,共5页
应用PCR扩增技术 ,克隆了甜菜夜蛾 (Spodopteraexigua)自然种群的部分钠离子通道基因片段 ,并对其进行了测序 .后将推断的氨基酸序列与其它敏感昆虫的相应序列进行了比较 ,发现在钠离子通道ⅡS6的 10 14同源位点上存在Leu(CTT)→Phe(TTT... 应用PCR扩增技术 ,克隆了甜菜夜蛾 (Spodopteraexigua)自然种群的部分钠离子通道基因片段 ,并对其进行了测序 .后将推断的氨基酸序列与其它敏感昆虫的相应序列进行了比较 ,发现在钠离子通道ⅡS6的 10 14同源位点上存在Leu(CTT)→Phe(TTT)点突变 ,表明该种群的抗药性可能与击倒抗性 (kdr) 展开更多
关键词 甜菜夜蛾 自然种群 除虫菊酯 L1014F突变 击倒抗性 钠离子通道 抗药性
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Joint Toxicity of Beauveria bassiana and Two Insect Growth Regulators on Beet Armyworm,Spodoptera exigua(Hübner) 被引量:3
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作者 Hu Anling Tian Zhaofeng +2 位作者 Gao Xiwu Nong Xiangqun Li Yongdan 《Plant Diseases and Pests》 CAS 2016年第5期1-4,12,共5页
Joint toxicity of Beauveria bassiana and two insect growth regulators, methoxyfenozide and hexaflumuron, on beet annywonn larvae, Spodoptera exigua ( Htthner), were investigated. An obvious synergism effect of the t... Joint toxicity of Beauveria bassiana and two insect growth regulators, methoxyfenozide and hexaflumuron, on beet annywonn larvae, Spodoptera exigua ( Htthner), were investigated. An obvious synergism effect of the two insect growth regulators with B. beauveria was observed. When the insect growth regulators at LC50 concentrations (25 × 10^-6 g/L for methoxyfenozide and 1 000 × 10^ -6 g/L for hexaflumuren) were mixed with B. bassiana at the volume ratio of 4: 1, the mixtures achieved the best efficacy. The co-toxicity co-efficient (CTC) of methexyfenozide and B. bass/ana was 285, and that of hexaflumuron and B. bass/ana was 208. Scanning electron microscope observation of the longitudinal section of S. exigua revealed obviously different patterns of integument in different treatment groups. 展开更多
关键词 Insect growth regulators Beauveria bassiana spodoptera exigua Joint toxicity
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Cloning and Sequencing of a Gene Encoding GOBP2 in the Antenna of Spodoptera exigua
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作者 WANG Gui-rong, GUO Yu-yuan, XU Guang and WU Kong-ming( The Institute of Plant Protection , Chinese Academy of Agricultural Sciences , Beijing 100094 ) 《Agricultural Sciences in China》 CAS CSCD 2002年第2期200-206,共7页
A pair of degenerate primers was designed, based on the comparison of five insects' GOBP2 gene sequences reported previously. A specific band (about 400bp in length) was amplified from cDNA of Spodoptera exigua an... A pair of degenerate primers was designed, based on the comparison of five insects' GOBP2 gene sequences reported previously. A specific band (about 400bp in length) was amplified from cDNA of Spodoptera exigua antenna and another specific band (about 2kb in length) was amplified from genomic DNA. The two segments were cloned into T-easy vector, respectively. Results of sequencing and structural analysis showed that the full-length of GOBP2Sexi ORF is 426bp, 141 amino acid residues were encoded. The predicted MW and pI are 16.07ku and 5.09 .respectively. There are six conservative Cys locus in the sequence, which is the typical characteristic of OBPs. GOBP2Sexi gene was inserted by two introns between amino acid residue 22 and 23 and between 82 and 83. The length of two introns is 160bp and 1403bp. Results of Northern blot showed that GOBP2 gene expressed specifically in the antenna of Spodoptera exigua, and the expression level is nearly equal in the antenna of male and female moths. The sequence was deposited in GenBank/EMBL and the accession number is AJ294809. 展开更多
关键词 spodoptera exigua ANTENNA GOBP2 Gene cloning
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Impact of Variegated Temperature, CO<SUB>2</SUB>and Relative Humidity on Survival and Development of Beet Armyworm <i>Spodoptera exigua</i>(Hubner) under Controlled Growth Chamber
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作者 Jagdish Jaba Suraj Prashad Mishra +1 位作者 Naveen Arora Rajendra Munghate 《American Journal of Climate Change》 2020年第4期357-370,共14页
Climate change will have a noteworthy bearing on survival, development, and population dynamics of insect pests. Therefore, we contemplated the survival and development of beet army worm, <em>Spodoptera exigua&l... Climate change will have a noteworthy bearing on survival, development, and population dynamics of insect pests. Therefore, we contemplated the survival and development of beet army worm, <em>Spodoptera exigua</em> under different temperatures, (15<span style="white-space:nowrap;">&#176;</span>C, 25<span style="white-space:nowrap;">&#176;</span>C, 35<span style="white-space:nowrap;">&#176;</span>C, and 45<span style="white-space:nowrap;">&#176;</span>C), CO<sub>2</sub> (350, 550, 750 ppm) and relative humidity (55%, 65%, 75% and 85%) regimes. Maximum larval and pupal weights were recorded in insects reared at 25<span style="white-space:nowrap;">&#176;</span>C. The growth of <em>S. exigua</em> was faster at 35<span style="white-space:nowrap;">&#176;</span>C (larval period 7.4 days and pupal period 4.5 days) than at lower temperatures. At 15<span style="white-space:nowrap;">&#176;</span>C, the larval period was extended for 61.4 days and there was no adult emergence from the pupae till 90 days. The <em>S. exigua</em> hatchling was absent at 45<span style="white-space:nowrap;">&#176;</span>C. The larval survival ranged from 31.6% - 57.2%, maximum survival was recorded at 25<span style="white-space:nowrap;">&#176;</span>C, and minimum at 45<span style="white-space:nowrap;">&#176;</span>C. The maximum (84.27%) and minimum adult emergence were recorded in insects reared at 25<span style="white-space:nowrap;">&#176;</span>C and 35<span style="white-space:nowrap;">&#176;</span>C respectively. Maximum fecundity (384.3 eggs/female) and egg viability (51.97%) were recorded in insects reared at 25<span style="white-space:nowrap;">&#176;</span>C. Larval and pupal periods increased with an increase in CO<sub>2</sub> concentration. The highest pupal weights (128.6 mg/larva) were recorded at 550 ppm. The highest larval survival (73.50%) was recorded at 550 ppm and minimum (37.00%) at 750 ppm CO<sub>2</sub>. Fecundity was the highest in insects reared at 550 ppm CO<sub>2</sub> (657.4 eggs/female), and the lowest at 750 ppm. Maximum larval and pupal weights were recorded in insects reared at 75% relative humidity (RH). The growth rate of<em> S. exigua</em> was faster at 85% RH than at lower RH. The larval survival ranged between 40.0% - 58.5%. Maximum adult emergence (88.91%) was recorded in insects reared at 75% RH and minimum at 85% RH. Maximum fecundity (447.6 eggs/female) and the highest egg viability (72.95%) were recorded in insects reared at 75% and 65% RH respectively. Elevated temperatures and relative moistness will diminish the life cycle, while hoisted CO<sub>2</sub> will drag the life expectancy. Therefore, there is a need for thorough assessment of the impact of climatic factors on the population dynamics of insect pests, crop losses, and sustainability of crop production. 展开更多
关键词 spodoptera exigua TEMPERATURE CO2 Relative Humidity and Climate Change
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Antiviral function of peptidoglycan recognition protein in Spodoptera exigua(Lepidoptera:Noctuidae)
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作者 Jie Li Jie Li +7 位作者 Zhaohao Jing Qianlong Yu Guiling Zheng Bin Zhang Longsheng Xing Huan Zhang Fanghao Wan Changyou Li 《Insect Science》 SCIE CAS CSCD 2023年第4期1092-1104,共13页
Peptidoglycan recognition proteins(PGRPs)are a class of molecules that play a critical role in insect immunity.Understanding the function of PGRPs is important to improve the efficiency of microbial insecticides.In th... Peptidoglycan recognition proteins(PGRPs)are a class of molecules that play a critical role in insect immunity.Understanding the function of PGRPs is important to improve the efficiency of microbial insecticides.In this study,we investigated the role of PGRP-LB(a long type PGRP)in insect immunity against viruses using Spodoptera exigua and Spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV)as an insect-virus model.We cloned and identified a PGRP-LB gene from S.exigua;the gene consisted of 7 exons that encoded a polypeptide of 234 amino acids with a signal peptide and a typical amidase domain.Expression analysis revealed that the abundance of SePGRP-LB transcripts in the fat body was greater than in other tissues.Overexpression of SePGRP-LB resulted in a significant decrease of 49%in the rate of SeMNPV-infected cells.In addition,the multiplication of SeMNPV was significantly decreased:a decrease of 79%in the production of occlusion-derived virion(ODV),and a maximum decrease of 50%in the production of budded virion(BV).In contrast,silencing of SePGRP-LB expression by RNA interference resulted in a significant 1.65-fold increase in the rate of SeMNPV-infected cells,a significant 0.54-fold increase in ODV production,a maximum 1.57-fold increase in BV production,and the larval survival dropped to 21%.Our findings show that SePGRP-LB has an antiviral function against SeMNPV,and therefore this gene may provide a target for lepidopteran pest control using virus insecticides. 展开更多
关键词 ANTIVIRAL long-type peptidoglycan recognition protein gene PGRP-LB spodoptera exigua multiple nucleopolyhedrovirus spodoptera exigua
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Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations 被引量:6
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作者 Huan Yu Zi-Qi Li +1 位作者 Yi-Yi Ou-Yang Guo-Hua Huang 《Insect Science》 SCIE CAS CSCD 2020年第6期1158-1172,共15页
Apoptosis plays critical roles in multiple biological processes in multicellular organisms.Caspases are known as important participators and regulators of apoptosis.Here,four novel caspase genes of Spodoptera exigua w... Apoptosis plays critical roles in multiple biological processes in multicellular organisms.Caspases are known as important participators and regulators of apoptosis.Here,four novel caspase genes of Spodoptera exigua were cloned and characterized which were designated as SeCasp-1,SeCasp-6,ScCasp-7 and SeCasp-S.Analysis of the putative encoded protein sequences of these SeCasps indicated that SeCasp-1 and ScCasp-7 were possible homologs of executor caspases;SeCasp-8 was a possible homolog of initiator caspases;and SeCasp-6 was a unique caspase of S.exigua that shares low similarity with all the identified insect caspases.Based on baculovirus expression system analyses,SeCasp-1 exhibited similar caspase activity to human caspase-1,-3,-4,-6,-8 and-9;SeCasp-6 presented similar caspase activity to human caspase-2,-3,-4,-6,-8 and-9;SeCasp-7 exhibited similar caspase activity to human caspase-2,-3 and-6;and SeCasp-8 presented similar caspase activity only to human caspase-8.Induction with diiTcrent chemicals revealed that SeCasp-1 showed extreme upregulation after 24 h in the treated fat body cell line(IOZCAS-Spex-II)of S.exigua.Developmental expression analysis revealed that SeCasp-1 was highly transcribed in the larval stages,while SeCasp-6,SeCasp-7,SeCasp-H were down-regulated.The in vivo detection of the relative expression levels of SeCasps in S.eixgua larvae inoculated with different pathogens suggested that SeCasp-J was sensitive to Bacillus thuringiensis infection and that SeCasp-6 was sensitive to baculovirus infection.SeCasp-7 and SeCasp-H showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection. 展开更多
关键词 apoptosis apoptotic inducing insect caspase SeCasp spodoptera exigua
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Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm,Spodoptera exigua(Hübner) 被引量:4
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作者 GUO JianYing WU Gang WAN FangHao 《Science China(Life Sciences)》 SCIE CAS 2011年第2期152-158,共7页
Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,Spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt co... Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,Spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt cotton for different time periods.Nutrient composition and specific enzyme activities differed significantly between the S.exigua fed Bt vs.non-Bt cotton.At 1,6 and 24 h,free fatty acid and glucose levels were significantly lower in S.exigua fed on Bt cotton than those fed on non-Bt cotton.S.exigua fed on Bt cotton had significantly higher trypsin and total superoxide dismutase(T-SOD) activities and significantly lower lipase,carboxylesterase and acetylcholinesterase activities than non-Bt fed worms for all feeding time periods.Differences were also observed among feeding times within each cotton variety group.Significantly lower free fatty acid and total amino acid were observed in S.exigua fed on Bt cotton for 24 h than in those fed for 1 h.Significantly lower activities of lipase and trypsin were detected in S.exigua fed on Bt cotton for 24 h than those for 1 and 4 h.However,carboxylesterase and acetylcholinesterase activities in S.exigua fed on Bt cotton for 24 h were significantly higher than those for 1,4 and 6 h.The interaction between cotton variety and feeding time significantly affected the activities of lipase,trypsin,acetylcholinesterase and T-SOD enzymes in S.exigua.Measuring the temporal allocation of protection and detoxification enzyme activities in the body of S.exigua in response to B.thuringiensis can provide a meaningful evaluation on the metabolic tolerance of herbivorous insects under the continuous selection pressure of a toxic protein. 展开更多
关键词 Bacillus thuringiensis spodoptera exigua detoxification enzyme CARBOXYLESTERASE ACETYLCHOLINESTERASE
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De novo transcriptome analysis of Spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV) genes in latently infected Se301 cells 被引量:2
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作者 Zheng Fang Jingxu Shao Qingbei Weng 《Virologica Sinica》 SCIE CAS CSCD 2016年第5期425-436,共12页
Cells of the P8-Se301-C1 strain are Spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus (SeMNPV) genome and which are resistant to homologous SeMNPV infect... Cells of the P8-Se301-C1 strain are Spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus (SeMNPV) genome and which are resistant to homologous SeMNPV infections. The cells produce no viral progeny, suggesting that the infection is a latent-like viral infection. To investigate the SeMNPV genes harbored in the P8-Se301-C1 cells, the de novo transcriptomes of P8-Se301-Cl cells and S. exigua Se301 cells were analyzed and compared. A total of 54,569,296 reads were obtained from the P8-Se301-C1 cells that yielded 112,565 final unigenes with a mean length of 1,093 nt. A total of 56,865,504 reads were obtained from the Se301 cells that yielded 102,996 final unigenes with a mean length of 1,082 nt. Ten SeMNPV gene transcripts (se5, se7, se8, se12, se43, se45, se89, se90, se124, and se126) were detected in the P8-Se301-C1 cells by RNA-Seq but not in the Se301 cells, which was verified by RT- PCR. 5'/3' RACE analyses showed that the 3'- or 5'-end sequences of the viral transcripts are aligned to the host gene sequences in P8-Se301-C1 cells, suggesting that the SeMNPV genes may integrate into and be transcribed with the host genes in the P8-Se301-C1 cells. Furthermore, six additional viral gene transcripts, sell, se42, se44, se88, se91, and se127 (incorporated into chimeric fusion transcripts in the P8-Se301-C1 cells), were detected in the RACE analyses. Taken together, sixteen SeMNPV transcripts were identified in the P8-Se301-C1 cell strain. This study provides information to develop the understanding of baculovirus latent infections and superinfection exclusion. 展开更多
关键词 RNA-SEQ SeMNPV BACULOVIRUS latent infection spodoptera exigua
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Characterization of a β-1,3-glucan recognition protein from the beet armyworm, Spodoptera exigua (Insecta: Lepidoptera: Noctuidae) 被引量:1
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作者 Kyeongrin Bang Sujin Park Saeyoull Cho 《Insect Science》 SCIE CAS CSCD 2013年第5期575-584,共10页
The β-1,3-glucan recognition protein gene from Spodoptera exigua (SeβGRP) was cloned and characterized. The cDNA of this gene is 1?644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa)... The β-1,3-glucan recognition protein gene from Spodoptera exigua (SeβGRP) was cloned and characterized. The cDNA of this gene is 1?644 nucleotides in length and the predicted polypeptide is 491 amino acids (aa) in length, with a calculated molecular mass of 54.8 kDa. The first 22 aa encode a predicted secretion signal peptide. A BLAST search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of SeβGRP revealed that this protein is most similar to the β-1,3-glucan recognition protein (βGRP) family of pattern recognition proteins. Using reverse-transcription polymerase chain reaction, we detected the presence of SeβGRP transcripts in the egg, larval, pupal, and adult stages of S. exigua. In addition, the SeβGRP transcript was expressed in all the tissues examined including the brain, hemocytes, fat body, intestine, and cuticle. There were no changes in SeβGRP mRNA levels in larvae infected with ultraviolet (UV)-killed Escherichia coli DH5α compared with the control larvae inoculated with the water; however, SeβGRP mRNA levels were markedly elevated 4–8 h after infection and slightly induced 12–24 h after infection in larvae injected with UV-killed Fusarium oxysporum. This may be because β-1,3-glucan is the main component of the cell wall of F. oxysporum, but not E. coli DH5α. 展开更多
关键词 β-1 3-glucan recognition protein Fusarium oxysporum immune spodoptera exigua
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Three metabolites from an entomopathogenic bacterium, Xenorhabdus nematophila, inhibit larval development of Spodoptera exigua (Lepidoptera: Noctuidae) by inhibiting a digestive enzyme, phospholipase A2
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作者 Jaehyun Kim Yonggyun Kim 《Insect Science》 SCIE CAS CSCD 2011年第3期282-288,共7页
An entomopathogenic bacterium, Xenorhabdus nematophila, has been known to induce significant immunosuppression of target insects by inhibiting immune-associated phospholipase A2 (PLA2), which subsequently shuts down... An entomopathogenic bacterium, Xenorhabdus nematophila, has been known to induce significant immunosuppression of target insects by inhibiting immune-associated phospholipase A2 (PLA2), which subsequently shuts down biosynthesis of eicosanoids that are critical in immune mediation in insects. Some metabolites originated from the bacterial culture broth have been identified and include benzylideneacetone, proline-tyrosine and acetylated phenylalanine-glycine-valine, which are known to inhibit enzyme activity of PLA2 extracted from hemocyte and fat body. This study tested their effects on digestive PLA2 of the beet armyworm, Spodoptera exigua. Young larvae fed different concentrations of the three metabolites resulted in significant adverse effects on larval development even at doses below 100 #g/mL. In particular, they induced significant reduction in digestive efficiency of ingested food. All three metabolites significantly inhibited catalytic activity of digestive PLA2 extracted from midgut lumen of the fifth instar larvae at a low micromolar range. These results suggest that the inhibitory activities of the three bacterial metabolites on digestive PLA2 of S. exigua midgut may explain some of their oral toxic effects. 展开更多
关键词 DIGESTION MIDGUT phospholipase A2 spodoptera exigua Xenorhabdus nematophila
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Molecular characterization and developmental expression of the gene encoding the prothoracicotropic hormone in the beet armyworm, Spodoptera exigua 被引量:3
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作者 XU Jun1,SU JianYa3, SHEN JinLiang3 & XU WeiHua1,2 1 Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China 2 State Key Laboratory of Biocontrol and Institute of Entomology, School of Life Sciences, Zhongshan University, Guangzhou 510275, China 3 Department of Plant Protection, Nanjing Agriculture University, Nanjing 210095, China 《Science China(Life Sciences)》 SCIE CAS 2007年第4期466-472,共7页
Prothoracicotropic hormone (PTTH), a neuropeptide hormone stimulating the prothoracic glands to synthesize ecdysone, plays an important role in regulating postembryonic development in insects. The cDNA encoding PTTH w... Prothoracicotropic hormone (PTTH), a neuropeptide hormone stimulating the prothoracic glands to synthesize ecdysone, plays an important role in regulating postembryonic development in insects. The cDNA encoding PTTH was isolated and sequenced from the beet armyworm, Spodoptera exigua (Spe). The deduced a?mino acid sequence is composed of a signal peptide, a peptide (65 amino acids) of un-known function, and a mature PTTH molecule (111 amino acids). The Spe-PTTH shows similarities (45.5%―70.3%) to other known PTTHs reported in Lepidoptera species, but 7 cysteine r?esidues and the hydrophobic regions were conserved. Whole-mount immunocytochemistry by using an antiserum against recombinant Helicoverpa armigera PTTH showed that Spe-PTTH was synthesized in two pairs of neurosecretory cells in the S. exigua brain. Northern blot analysis demonstrates the presence of a 1.2-kb transcript in the brain. The Spe-PTTH mRNA is detectable at high levels at the wandering larval stage, early pupal stage, and pharate adult stage, suggesting that the Spe-PTTH gene might be corre-lated with molting, metamorphosis, and reproduction. 展开更多
关键词 prothoracicotropic HORMONE molecular characterization Northern blot WHOLE-MOUNT IMMUNOCYTOCHEMISTRY spodoptera exigua
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两种昆虫生长调节剂类杀虫剂对甜菜夜蛾幼虫储存蛋白含量的影响
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作者 张天澍 袁永达 +3 位作者 常晓丽 滕海媛 顾浩天 王冬生 《上海农业学报》 2024年第3期57-61,共5页
通过阴离子交换、凝胶过滤等方法对甜菜夜蛾5龄幼虫储存蛋白进行纯化,采用SDS-PAGE、ELISA等方法测定甜菜夜蛾储存蛋白动态变化,研究两种昆虫生长调节剂类杀虫剂甲氧虫酰肼和氟啶脲在亚致死剂量下对储存蛋白含量的影响。结果表明:甜菜... 通过阴离子交换、凝胶过滤等方法对甜菜夜蛾5龄幼虫储存蛋白进行纯化,采用SDS-PAGE、ELISA等方法测定甜菜夜蛾储存蛋白动态变化,研究两种昆虫生长调节剂类杀虫剂甲氧虫酰肼和氟啶脲在亚致死剂量下对储存蛋白含量的影响。结果表明:甜菜夜蛾幼虫储存蛋白亚基分子量为85.1 ku。在5龄幼虫血淋巴中储存蛋白质量浓度随日龄逐渐增加,于5龄第3天达到最大值30.76 mg∕mL。低剂量的甲氧虫酰肼能够提高幼虫血淋巴中储存蛋白的浓度,处理48 h和72 h后,LC10处理组的储存蛋白质量浓度分别为19.27 mg∕mL和45.21 mg∕mL,显著高于对照组。高剂量的甲氧虫酰肼对甜菜夜蛾储存蛋白存在着明显的抑制作用,LC40处理组在72 h后,血淋巴中储存蛋白浓度仅为对照组的48.47%。氟啶脲LC10、LC40两个处理组的储存蛋白浓度在各个处理时间均低于对照组,特别是在处理48 h和72 h后,血淋巴中储存蛋白浓度显著降低,且氟啶脲浓度越高,影响越显著,LC40处理组在72 h后,血淋巴中储存蛋白浓度仅为对照组的26.77%。研究结果可为进一步揭示两种杀虫剂对甜菜夜蛾幼虫的毒力作用提供理论依据。 展开更多
关键词 甜菜夜蛾 储存蛋白 甲氧虫酰肼 氟啶脲
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异色瓢虫对甜菜夜蛾和草地贪夜蛾的捕食潜能
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作者 吴钰薇 郑林浩 +5 位作者 高鹏 朱立贺 张国春 李飞武 夏蔚 徐文静 《植物保护》 CAS CSCD 北大核心 2024年第5期171-179,共9页
为明确异色瓢虫Harmonia axyridis对甜菜夜蛾Spodoptera exigua和草地贪夜蛾Spodoptera frugiperda的捕食潜能,本文在室内条件下研究了异色瓢虫对上述2种鳞翅目害虫2龄幼虫的捕食功能反应和搜寻效应;在网室条件下测定了异色瓢虫对2种害... 为明确异色瓢虫Harmonia axyridis对甜菜夜蛾Spodoptera exigua和草地贪夜蛾Spodoptera frugiperda的捕食潜能,本文在室内条件下研究了异色瓢虫对上述2种鳞翅目害虫2龄幼虫的捕食功能反应和搜寻效应;在网室条件下测定了异色瓢虫对2种害虫低龄幼虫的防控效果。室内研究结果表明,异色瓢虫雌雄成虫对这2种害虫2龄幼虫的捕食功能反应均属于HollingⅡ型圆盘方程,其中雌成虫对甜菜夜蛾和草地贪夜蛾2龄幼虫的瞬时攻击率(a)分别为1.120和0.987,控害效能(a/T_(h))分别为134.514头和154.408头,日最大捕食量(1/T_(h))分别为120.124头和156.437头;雄成虫对甜菜夜蛾和草地贪夜蛾2龄幼虫的瞬时攻击率(a)分别为1.071和0.830,控害效能(a/T_(h))分别为116.262头和124.568头,日最大捕食量(1/T_(h))分别为108.540头和150.039头。网室试验结果表明,2∶300、4∶300和8∶300的益害比下异色瓢虫均能有效控制甜菜夜蛾和草地贪夜蛾低龄幼虫的数量,释放后8 d防效分别在65%、80%和95%以上。综上所述,异色瓢虫具备对甜菜夜蛾和草地贪夜蛾低龄幼虫有效控害的潜力,试验结果可为利用异色瓢虫防控甜菜夜蛾和草地贪夜蛾提供理论依据。 展开更多
关键词 异色瓢虫 甜菜夜蛾 草地贪夜蛾 捕食功能反应 生物防治
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