Analysis on Survival and Prognostic Factors in Patients with Resectable Pancreatic Adenocarcinoma

Survival after pancreatic cancer surgery is extremely unfavorable even after curative resection. Prognostic factors have been explored but remain largely undefined. The present study was to identify the role of clinical and laboratory variables in the prognostic significance of resectable pancreatic adenocarcinoma. A total of 96 patients who underwent curative resection for pancreatic cancer were included. Survival was evaluated based on complete follow-up visits and was associated with potential prognostic factors using the Kaplan-Meier method and Cox proportional hazard model survival analyses. The results showed that prognostic variables significantly reduced survival, including old age, poorly differentiated tumors, elevated tumor markers and positive lymph node metastasis（LNM）. Age of older than 60 years（HR=1.83, P=0.04）, LNM（HR=2.22, P=0.01）, lymph node ratio（00.2, HR=1.92, P=0.017）, initial CA199（HR=4.80, P=0.004）, and CEA level（HR=2.59, P=0.019） were identified as independent prognostic factors by multivariate analysis. It was concluded that LNR may be potent predictor of survival and suggests that surgeons and the pathologists should thoroughly assess lymph nodes prior to surgery.

Tissue Culture and Rapid Propagation System of Roselle(Hibiscus sabdariffa L.)

[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle.

Propagation Experiment of Twig Segmentation Cutting of Rosa damascene Mill.

Five different parts of Rosa damascene Mill. twigs were treated with hormone solution （50 mg/L NAA＋100 mg/L IBA） and planted in base material. After 50 d of growth, the survival rate and rooting condition were investigated. The results showed that the survival rate from 5 different parts of cutting stem decreased from top to bottom （upper 98.22%, middle and upper 95.12%, middle 91.10%, middle and lower 72.90%, lower 71.12%）. The same results were also observed in the number, total length and fresh weight of secondary roots of cutting segments from different parts of twigs.

Prognostic value of lymph node ratio in hypopharyngeal squamous cell carcinoma after chemoradiotherapy

Background Lymph node ratio （LNR） has recently been reported as a potential prognostic marker in many malignant diseases. We aimed to analyze the potential prognostic effect of LNR on hypopharyngeal squamous cell carcinoma （HPSCC） after neoadjuvant therapy in our institution.