Objective: To study the effects of occlusal trauma on the ultra structure of synovial membrane and articular cartilage in rabbits temporomandibular joints (TMJ). Methods: TMJs from six rabbits with occlusal trauma and...Objective: To study the effects of occlusal trauma on the ultra structure of synovial membrane and articular cartilage in rabbits temporomandibular joints (TMJ). Methods: TMJs from six rabbits with occlusal trauma and three control rabbits were studied by transmission electron microscopy. Results: Degenerative changes in synovial membrane and articular cartilage of TMJ were induced following occlusal trauma. The structure of the articular surface was damaged, and chondrocytes in cartilage showed signs of degeneration. The synovial lining cells contained dense accumulations of vimentin intermediate filaments (IFs), which were especially prevalent in the cellular processes as well as paranuclearly. Microvilli on the synovial cell membrane were commonly seen. The “vermiform bodies” in the deeper interstitium of the synovial tissue were also found. Our findings of the punctate adherens between synovial lining cells were described in detail. Conclusions: The occlusal trauma is really a factor inducing degenerative changes of the TMJ.展开更多
This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes...This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes of patients was deter- mined by MTT chromatometry and the apoptosis of these cells was assayed with TUNEL flow cytometry. It was found in this experiment that the degree of cell proliferation of the Res-treated group with dosages of 50-400 μM was significantly reduced in comparison with that of the control group, but percentage of the apoptotic cells demonstrated with TUNEL labeling was el- evated under treatment with Res at the same dosages in a concentration-dependent manner. The difference between the Res- treated group and the control group was quite significant ( P < 0.01). It is concluded that Res shows a potent anti-prolifera- tive effect on synoviocytes of patients with RA with induction of cell apoptosis, and it is likely a valuable candidate for the chemotherapy and management of patients with RA.展开更多
Background Synovium-derived stem cells （SDSCs） with higher chondrogenic potential are attracting considerable attention as a cell source for cartilage regeneration. We investigated the effect of bone morphogenetic p...Background Synovium-derived stem cells （SDSCs） with higher chondrogenic potential are attracting considerable attention as a cell source for cartilage regeneration. We investigated the effect of bone morphogenetic protein 2 （BMP-2） on transforming growth factor beta3 （TGF-β3）-induced chondrogenesis of SDSCs isolated from human osteoarthritic synovium in a pellet culture system. Methods The clonogenicity, stem cell marker expression and multi-differentiation potential of isolated SDSCs were determined by colony forming unit assay, flow cytometry and specific staining including alizarin red S, Oil red O and alcian blue staining, respectively. SDSCs pellet was cultured in chondrogenic medium with or without TGF-β3 or/and BMP-2. At day 21, the diameter and the weight of the pellets were measured. Chondrogenic differentiation of SDSCs was evaluated by Safranin O staining, immunohistochemical staining of collagen type Ⅱ, sulfated glycosaminoglycan （sGAG） synthesis and mRNA expression of collagen type Ⅱ, aggrecan, SOX9, link-protein, collagen type X and BMP receptor Ⅱ. Results Cells isolated under the optimized culturing density （104/60 cm2） showed clonogenicity and multi-differentiation potential. These cells were positive （〉99%） for CD44, CD90, CD105 and negative （〈10%） for CD34 and CD71. SDSCs differentiated to a chondrocytic phenotype in chondrogenic medium containing TGF-β3 with or without BMP-2. Safranin O staining of the extracellular matrix was positive and the expression of collagen type Ⅱ was detected. Cell pellets treated with TGF-β3 and BMP-2 were larger in diameter and weight, produced more sGAGs, and expressed higher levels of collagen type Ⅱ and other chondrogenic markers, except COL10A1, than medium with TGF-β3 alone. Conclusions SDSCs could be isolated from human osteoarthritic synovium. Supplementation with BMP-2 significantly promoted the in vitro TGF-β3-induced chondrogenic differentiation of SDSCs.展开更多
Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adju...Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL1, TNF-α bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured. Results: TBL could significantly inhibit the synovial fibroblast proliferation (P<0.001), and downregulate IL1, TNFα and PGE 2 productions (P<0.001); indomethacin could obviously promote the synovial fibroblast proliferation(P< 0.001 ). It significantly inhibited PGE 2 production, but further upregulated IL1 and TNFα secreted by synoviocytes (P<0.01). Conclusion: The therapeutical effect of TBL on AA might be associated with its downregulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.展开更多
文摘Objective: To study the effects of occlusal trauma on the ultra structure of synovial membrane and articular cartilage in rabbits temporomandibular joints (TMJ). Methods: TMJs from six rabbits with occlusal trauma and three control rabbits were studied by transmission electron microscopy. Results: Degenerative changes in synovial membrane and articular cartilage of TMJ were induced following occlusal trauma. The structure of the articular surface was damaged, and chondrocytes in cartilage showed signs of degeneration. The synovial lining cells contained dense accumulations of vimentin intermediate filaments (IFs), which were especially prevalent in the cellular processes as well as paranuclearly. Microvilli on the synovial cell membrane were commonly seen. The “vermiform bodies” in the deeper interstitium of the synovial tissue were also found. Our findings of the punctate adherens between synovial lining cells were described in detail. Conclusions: The occlusal trauma is really a factor inducing degenerative changes of the TMJ.
文摘This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes of patients was deter- mined by MTT chromatometry and the apoptosis of these cells was assayed with TUNEL flow cytometry. It was found in this experiment that the degree of cell proliferation of the Res-treated group with dosages of 50-400 μM was significantly reduced in comparison with that of the control group, but percentage of the apoptotic cells demonstrated with TUNEL labeling was el- evated under treatment with Res at the same dosages in a concentration-dependent manner. The difference between the Res- treated group and the control group was quite significant ( P < 0.01). It is concluded that Res shows a potent anti-prolifera- tive effect on synoviocytes of patients with RA with induction of cell apoptosis, and it is likely a valuable candidate for the chemotherapy and management of patients with RA.
文摘Background Synovium-derived stem cells （SDSCs） with higher chondrogenic potential are attracting considerable attention as a cell source for cartilage regeneration. We investigated the effect of bone morphogenetic protein 2 （BMP-2） on transforming growth factor beta3 （TGF-β3）-induced chondrogenesis of SDSCs isolated from human osteoarthritic synovium in a pellet culture system. Methods The clonogenicity, stem cell marker expression and multi-differentiation potential of isolated SDSCs were determined by colony forming unit assay, flow cytometry and specific staining including alizarin red S, Oil red O and alcian blue staining, respectively. SDSCs pellet was cultured in chondrogenic medium with or without TGF-β3 or/and BMP-2. At day 21, the diameter and the weight of the pellets were measured. Chondrogenic differentiation of SDSCs was evaluated by Safranin O staining, immunohistochemical staining of collagen type Ⅱ, sulfated glycosaminoglycan （sGAG） synthesis and mRNA expression of collagen type Ⅱ, aggrecan, SOX9, link-protein, collagen type X and BMP receptor Ⅱ. Results Cells isolated under the optimized culturing density （104/60 cm2） showed clonogenicity and multi-differentiation potential. These cells were positive （〉99%） for CD44, CD90, CD105 and negative （〈10%） for CD34 and CD71. SDSCs differentiated to a chondrocytic phenotype in chondrogenic medium containing TGF-β3 with or without BMP-2. Safranin O staining of the extracellular matrix was positive and the expression of collagen type Ⅱ was detected. Cell pellets treated with TGF-β3 and BMP-2 were larger in diameter and weight, produced more sGAGs, and expressed higher levels of collagen type Ⅱ and other chondrogenic markers, except COL10A1, than medium with TGF-β3 alone. Conclusions SDSCs could be isolated from human osteoarthritic synovium. Supplementation with BMP-2 significantly promoted the in vitro TGF-β3-induced chondrogenic differentiation of SDSCs.
文摘Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL1, TNF-α bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured. Results: TBL could significantly inhibit the synovial fibroblast proliferation (P<0.001), and downregulate IL1, TNFα and PGE 2 productions (P<0.001); indomethacin could obviously promote the synovial fibroblast proliferation(P< 0.001 ). It significantly inhibited PGE 2 production, but further upregulated IL1 and TNFα secreted by synoviocytes (P<0.01). Conclusion: The therapeutical effect of TBL on AA might be associated with its downregulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.
