经皮穿刺后TOM针校正法在侧卧位PELD手术中的运用

探讨经皮穿刺后TOM针校正法在椎间孔镜手术(percutaneousendoscopiclumbardiscectomy,PELD)手术中的价值。方法 分析2020年8月至2022年4月89例因单节段腰椎间盘突出症(Lumbardischerniation,LDH)入院后行PELD手术的患者资料,将52例行经皮穿刺后TOM针校正法建立通道的患者为A组,37例行传统经皮穿刺建立通道的患者作为B组。记录两组患者的一般情况、突出节段、穿刺次数、穿刺时间、透视次数、手术总体时间、术前和术后患者疼痛视觉模拟评分(VisualAnalogueScore,VAS)、日本骨科学会评分(JapaneseOrthopedicAssociation,JOA)评估比较术后疗效。结果 A组的穿刺次数、穿刺时间、术中透视次数及手术总时间均低于B组(P<0.05)。疗效方面,两组VAS、JOA评分均较术前有着明显的改善差异有统计学意义(P<0.05),两组在同一时期的随访无明显差异(P>0.05)。结论 与传统穿刺相比,经皮穿刺后TOM针校正法可做到靶点的准确穿刺,缩短了手术时长和透视次数,方法 操作简单,易懂易学,值得临床推广应用。

荷花MYB蛋白靶基因NnTOM1的克隆及功能分析

【目的】从荷花(Nelumbo nucifera)中克隆NnTOM1基因,并进行生物信息学分析、基因表达模式分析以及烟草遗传转化,为进一步研究NnTOM1基因的功能奠定基础。【方法】通过克隆获得NnTOM1基因,对其蛋白序列进行理化性质和保守结构域预测等生物信息学分析,利用荧光定量PCR(qRT-PCR)技术分析其组织特异性表达,并利用烟草遗传转化初步验证其生物学功能。【结果】NnTOM1基因编码区(Coding squence,CDS)长度为1191 bp,编码396个氨基酸,蛋白质分子式为C_(1918)H_(3130)N_(560)O_(589)S_(16),二级结构主要由α-螺旋和无规则卷曲组成,含有TOM家族保守结构域(VHS-ENTH-ANTH和GAT),定位于细胞质中;多重序列比对及系统进化树分析表明,其与澳洲坚果(Macadamia integrifolia)、蒂罗花(Telopea speciosissima)、博落回(Macleaya cordata)亲缘关系较近且序列一致性较高;qRT-PCR结果显示,NnTOM1基因在瓣化雄蕊中表达量最高,在根中表达量最低,且在重瓣型品种中表达量比单瓣型品种高;与野生型相比,NnTOM1转基因株系的花瓣颜色加深,花瓣先端变尖,花瓣间隔处加宽,花丝变长且明显长于花柱;且OE-1株系比野生型多出1片花瓣,表现出6片花瓣的表型。【结论】荷花NnTOM1基因可能参与荷花花器官的发育,为后续解析NnTOM1基因的功能提供了初步依据,也为荷花新品种选育提供了新的基因资源。

炎症微环境中TOM40对牙髓成纤维细胞线粒体呼吸功能的影响

目的:探究炎症微环境中线粒体外膜转位酶(TOM)复合体亚基TOM40对牙髓成纤维细胞线粒体呼吸功能的影响。方法:开髓暴露法构建大鼠磨牙牙髓炎模型,苏木精-伊红染色观察牙髓坏死面积;免疫组化染色观察牙髓组织促炎因子白介素(IL)-1β、抗肿瘤坏死因子(TNF)-α及DNA氧化损伤标志物8-OHDG的表达情况;TUNEL法检测牙髓组织细胞凋亡情况。体外培养人牙髓成纤维细胞(hDPFs),分别以1、5、10μg/mL脂多糖(LPS)炎性刺激,实时荧光定量PCR及Western blot检测细胞促炎因子IL-1β、TNF-α及促凋亡因子Bax、Bak的表达情况;通过TMRE、DCFH-DA探针及腺苷三磷酸(ATP)含量检测试剂盒检测hDPFs线粒体呼吸功能变化;实时荧光定量PCR筛选TOM复合体中表达下调亚基,并通过免疫荧光染色加以验证;基于筛选结果构建TOM40过表达hDPFs细胞株,并加入LPS刺激,检测线粒体呼吸功能变化。结果:大鼠磨牙牙髓炎模型中,随着暴露时间延长,牙髓坏死面积增大,IL-1β、TNF-α及8-OHDG表达升高,细胞凋亡增多(P<0.05)。炎症hDPFs模型中,LPS浓度增加,IL-1β、TNF-α、Bax及Bak蛋白和基因表达升高,线粒体呼吸功能受损,TOM40表达特异性下调(P<0.05);而过表达TOM40可有效改善LPS刺激导致的hDPFs线粒体膜电位下降、活性氧积累与ATP含量下降(P<0.05)。结论:牙髓炎的发展过程伴随牙髓组织氧化应激与hDPFs线粒体呼吸功能障碍,过表达TOM40可挽救hDPFs受损的线粒体呼吸功能。

会话含意理论视角下人物对话的理解和翻译——以The Adventures of Tom Sawyer及其汉译本为例

The Adventures of Tom Sawyer是美国著名现实主义作家马克·吐温于19世纪70年代创作的重要作品之一,小说以对话为主,语言幽默风趣。本文基于合作原则和会话含意理论,以The Adventures of Tom Sawyer及成时汉译本为研究对象,通过对比英语原文和汉语译文,考察小说人物对话“会话含意”传递过程中所体现的翻译技巧。研究发现,增译、转换、分译、减译均能成功传递会话含意,而译者对翻译技巧的选择则受到译者认知、读者取向、英汉语言和文化差异等多重因素的综合影响。The Adventures of Tom Sawyer is one of the major works written by the famous American realist writer Mark Twain in the 1870s. The novel is dialogue-based with humorous language. Based on the cooperative principle and the conversational implicature theory, this study takes The Adventures of Tom Sawyer and its Chinese translation by Cheng Shi as the research object and investigates the translation techniques embodied in the transmission of “conversational implicature” of characters’ utterances by comparing the original English text and the Chinese translation. It is found that addition, shift, division and omission can all convey conversational implicature, and that the translator’s choice of translation techniques is influenced by multiple factors, such as translator’s cognition, readers’ orientation, and English-Chinese linguistic and cultural differences.

礼貌原则的违反在儿童文学对话中的运用和翻译——以The Adventures of Tom Sawyer为例

本文以The Adventures of Tom Sawyer英文原著以及成时的中文译本为研究对象,借鉴利奇(Geoffrey Leech)的礼貌原则为理论支撑,基于儿童文学的独特性,探讨了儿童文学中礼貌原则的违反现象,研究发现成时在翻译过程中通过添加语气、增强情感和明晰内涵等策略有效传达“不礼貌”话语的语用意义。本研究旨在为提升儿童文学翻译质量及跨文化交流提供参考意义。

尖孢镰刀菌粘团专化型线粒体外膜转运酶亚基Tom7基因敲除突变体的构建与表型分析

【目的】研究线粒体外膜转运酶亚基Tom7基因在尖孢镰刀菌粘团专化型中的功能。【方法】利用同源重组原理,采用PEG介导原生质体的转化方法获得基因敲除突变体,并分析其表型和致病力。【结果】敲除Tom7基因导致尖孢镰刀菌的生长速率减慢,菌丝出现波浪状弯曲生长,产孢量增加,对氧胁迫和渗透胁迫敏感,但对其毒力无影响。【结论】由此推测线粒体外膜转运酶亚基Tom7基因缺失后,影响线粒体蛋白转运功能,进一步对尖孢镰刀菌的生长发育和产孢造成影响。

Impact of Different Light Characteristics on the Growth and Lipid Content of Diatom Phaeodactylum tricornutum Transconjugant Strains

Light regulates important metabolic processes in microalgal cells, which can further impact the metabolism and the accumulation of biomolecules such as lipids, carbohydrates, and proteins. Different characteristics of light have been studied on various strains of the model diatom Phaeodactylum tricornutum, but not on transconjugant cells and information on wild-type strains is still limited. Therefore, we studied the impact of different light characteristics such as spectral quality, light intensity and light shift on the growth, and the composition in lipids and fatty acids of P. tricornutum cells to provide a comprehensive context for future applications. Initially, we tested the impact of spectral quality and light intensity on P. tricornutum transformed with an episomal vector (Ptev), harboring the resistance gene Sh ble. Results indicated that Ptev cells accumulated more biomass and overall lipids in spectral quality Red 1 (R1: 34% > 600 nm > 66%) more effectively as compared to Red 2 (R2: 8% > 600 nm > 92%). It was also detected that cell granularity was higher in R1 as compared to R2. Furthermore, by testing two light intensities 65 μmol·m-2·s-1 and 145 μmol·m-2·s-1 light, it was observed that 145 μmol·m-2·s-1 led to an increase in growth trend, total biomass and lipid content. Combining spectral qualities and light intensities, we show that the lipid accumulation raised by 2.8-fold. Studying the light intensity and spectral quality allowed us to optimize the light conditions to R1 spectral quality and light intensity 145 μmol·m-2·s-1. These initial results showed that red light R1 at 145 μmol·m-2·s-1 was the best condition for biomass and total lipids accumulation in Ptev cells. Next, we further combined these two-light optimizations with a third light characteristics, i.e. light shift, where the cultures were shifted during the early stationary phase to a different light environment. We studied Red light shift (Rs) to investigate how light condition variations impacted P. tricornutum transconjugants Ptev and with an episomal vector containing the reporter gene YFP (PtYFP). We observed that Rs induced growth and fatty acid eicosapentaenoic acid (EPA) in Ptev as compared to PtYFP. Altogether, the study shows that red light shift of R1 at 145 μmol·m-2·s-1 promoted biomass and total lipids accumulation in Ptev and PtYFP cells. The study provides a comprehensive approach to using different light characteristics with the aim to optimize growth and lipids, as well as to fatty acid production.

Risk Factors for Severity and Mortality of Patients Hospitalized for COVID-19 during the 3rd Wave of the Epidemic-Sao Tome and Principe

Rationale: In the literature, some risk factors for severity and mortality from COVID-19 have been indicated. However, these factors can change, depending on the characteristics of the population and health services. In this sense, longitudinal studies can be useful for understanding local realities and subsidizing health actions based on these realities. Objective: To analyze the risk factors for severity and death in hospitalized patients with COVID-19. Methods: A retrospective cohort of patients with COVID-19 hospitalized from August 1 to October 16, 2021 (3rd wave of the pandemic), notified by the Department of Epidemiological Surveillance of Sao Tome and Principe. We employed measures of strength of associations for the analysis of exposure risk factors. Results: We analyzed 110 hospitalized patients (31.8% severe-critical and 68.2% non-severe). The risk factors for severe forms of COVID-19 were: being aged ≥60 years (RR = 3.3), being male (RR = 2), having comorbidities (RR = 2) and the risk increases to 10-fold for multicomorbidities, with emphasis on obesity, neoplasia, skin-muscle-surgical infection, dementia and to some degree CVD. 62.9% of patients with severe forms of the disease were not vaccinated. Risk factors for death among hospitalized and severe/critical cases, respectively, were having comorbidities (RR = 8 and 2.4) multicomorbidities (RR = 10 and 2.8 for those with 2 comorbidities and RR = 33.3 and 4 for those with 3 or 4 comorbidities), especially diabetes, dementia, neoplasia, cutaneous-muscular infection, and obesity. Although CVD was not associated with risk factors for death, these were the most frequently found among the severely hospitalized and deaths. In addition, important risk factors associated with death were not using corticoids (RR = 3.3, 230-fold risk) and not using anticoagulants-heparin (RR = 1.3, 30% risk) more compared to the severe cases that did use them. Most of the patients who died (63.2%) were not vaccinated. Moreover, having only 1 dose of the vaccine was a risk factor 1.9 times more for death among all hospitalized patients, but in the severe cases, there was no association between the variable vaccination and death. Among those hospitalized with 2 doses, it was a 0.5-fold protective factor among those hospitalized. The Delta variant of Sarscov-2 was the one found among severe cases and deaths investigated by genetic sequencing, with more exuberant clinical features compared to the other 2 previous vaccinations. Conclusion: Being elderly, male and presenting comorbidities, mainly multicomorbidities were the main characteristics associated with severity of COVID-19. On the other hand, comorbidities, and even worse, multicomorbidities, hospitalization for respiratory failure, lowered level of consciousness, no use of corticoid and no use of anticoagulation in critically ill patients, and not having at least 2 doses of vaccine for covid-19, were characteristics associated with death by COVID-19. These results will help inform healthcare providers so that the best interventions can be implemented to improve outcomes for patients with COVID-19. Public health interventions must be carefully tailored and implemented in these susceptible groups to reduce the risk of mortality in patients with COVID-19 and then the risk of major complications. Intensive and regular follow-up is needed to detect early occurrences of clinical conditions.

Statistical Tests of the Validation of TCO Satellite Measurements, Recorded Simultaneously by TOMS-OMI (2005) and OMI-OMPS (2012-2018)

Two statistical validation methods were used to evaluate the confidence level of the Total Column Ozone (TCO) measurements recorded by satellite systems measuring simultaneously, one using the normal distribution and another using the Mann-Whitney test. First, the reliability of the TCO measurements was studied hemispherically. While similar coincidences and levels of significance > 0.05 were found with the two statistical tests, an enormous variability in the levels of significance throughout the year was also exposed. Then, using the same statistical comparison methods, a latitudinal study was carried out in order to elucidate the geographical distribution that gave rise to this variability. Our study reveals that between the TOMS and OMI measurements in 2005 there was only a coincidence in 50% of the latitudes, which explained the variability. This implies that for 2005, the TOMS measurements are not completely reliable, except between the -50° and -15° latitude band in the southern hemisphere and between +15° and +50° latitude band in the northern hemisphere. In the case of OMI-OMPS, we observe that between 2011 and 2016 the measurements of both satellite systems are reasonably similar with a confidence level higher than 95%. However, in 2017 a band with a width of 20° latitude centered on the equator appeared, in which the significance levels were much less than 0.05, indicating that one of the measurement systems had begun to fail. In 2018, the fault was not only located in the equator, but was also replicated in various bands in the Southern Hemisphere. We interpret this as evidence of irreversible failure in one of the measurement systems.

TOM与中国面条煮面品质的关系

本文以面条感官鉴定方法(SB/T10137—93)和用于鉴定意大利实心细面条(Spaghetti)品质的煮熟面条冲洗水中全有机物含量(TOM)的方法鉴定了30个普通小麦品种(系)的面条煮面品质及煮熟面条冲洗水中有机物含量(TOM),分析了TOM与中国面条煮面品质的关系。结果表明:TOM与感官鉴定总评分及除适口性和食味外的其它分项密切相关,TOM也可以用于鉴定中国面条煮面品质。本文还探讨了把TOM作为中国面条煮面品质指标的选择标准。

用TOM评价中国面条品质的新方法及研究小麦品质对它的影响

本文以面条品尝鉴定方法（ＳＴ／Ｔ１０１３７－９３）和用于鉴定细条实心面（Ｓｐａｇｈｅｔｉ）品质的煮熟面条冲洗水中总有机物含量（ＴＯＭ）的方法，分别鉴定了２４个普通小麦品种（系）的面条煮面的品质，并研究了煮面品质与小麦品种籽粒及面粉的８个品质性状之间的相关关系。结果表明面条品尝鉴定评分与ＴＯＭ值之间有高度相关性。籽粒蛋白质含量与ＴＯＭ值呈显著负相关；蛋白组分中醇溶蛋白含量与ＴＯＭ值显著负相关；沉降值与ＴＯＭ值呈极显著负相关。通径分析表明，全蛋白含量对ＴＯＭ值的直接影响最大，剩余蛋白含量次之，醇溶蛋白含量列第三位；高分子量麦谷蛋白亚基组成与ＴＯＭ值间无明显关系；中分子量麦谷蛋白亚基组成与ＴＯＭ值关系密切，具有３８．８８ＫＤ亚基、５３．２５ＫＤ亚基或共有这两个亚基的品种，其面条ＴＯＭ值较低，面条品质优良。

王雷雷的tom进行曲

从艰难入市，到短信发家，再到玩转电信增值服务和紧跟3G，在王雷雷的时光进行曲中，人们看到了TOM在线的无线互联网门户之路。

2007年十大影响力企业之TOM在线私有化

自从中国移动于2006年7月7日推出一系列对SP的整改措施以来，整个移动无线增值行业就集体步入了低谷，这场行业灾难TOM当然也难以逃脱。TOM集团表示，TOM在线自2004年3月首次公开售股以来，已经成为领先的无线增值服务商之一，其无线增值服务已经占到公司总收入的90％以上。TOH（www．tom．com．cn）在线在中国移动该系列规定施行的第一个财季收入和利润便较上一年度同期分别减少15％和59％。

农杆菌介导的番茄Micro-Tom遗传转化体系的建立

Micro-Tom番茄植株矮小,生长密度高,生命周期短,转化效率高,成为功能基因组学研究的新型模式植物。对影响Micro-Tom遗传转化频率的共培养时间、AS的添加、工程菌液浓度和抑制农杆菌所用抗生素种类进行了分析,建立了Micro-Tom稳定高效的遗传转化体系。以bar基因设计引物对转化群体进行PCR检测,阳性率为72.3%,平均插入位点数为1.8个。遗传体系的建立为Micro-Tom在植物生物学研究中提供理论基础。

利用TOMS资料遥感沙尘暴的研究

研究了利用TOMS资料定量遥感沙尘暴的方法 ,并利用该方法遥感了 1998年 4月 15日发生在中蒙边境地区的一次沙尘暴过程 ,得到了沙尘暴的发生、发展、强度以及影响范围等特征。结果表明 ,该方法基本上可以排除云的影响 ,能够定量遥感沙尘暴的动态发展过程 ,是一个较好的沙尘暴遥感方法。

利用EP/TOMS遥感资料分析我国上空沙尘天气过程

利用EPTOMS卫星遥感资料,并结合地面气象观测记录,分析了影响我国典型沙尘暴天气的发生、发展和传输过程。定义了使用EPTOMS气溶胶指数定量描述沙尘天气强度的指标体系,并对1998年3—4月间发生的沙尘天气的强度及其演变进行了详细的分析。结果表明:利用EPTOMS气溶胶指数并结合气象观测资料,可以对大规模的沙尘天气进行及时判别、监视,并预报影响范围及传输路径;同时,利用TOMS气溶胶指数建立起来的指标体系可以半定量化地描述沙尘暴天气的强度和影响范围。

Micro-Tom番茄矮化微型机制及其在植物功能基因组学研究中的应用

Micro-Tom番茄植株矮小,生长密度高,生命周期短,容易被高效转化,成为功能基因组学研究的新型模式植物。文章对Micro-Tom的由来和生物学特征作了概述,重点阐述了Micro-Tom矮化微型的遗传机制以及该番茄在功能基因组学研究中的应用和遗传转化体系研究的进展。

基于TOM匹配的反舰导弹抗冲淡干扰搜捕策略

为了对抗雷达导引头受到的箔条冲淡干扰,将TOM匹配技术应用于反舰导弹搜捕中。首先简要介绍了TOM匹配应用于搜捕的优势,然后定义了用于反舰导弹搜捕的TOM表示方法与发射装订,最后根据定义的导引头观测TOM提出了TOM匹配方法和目标选择准则,实现反舰导弹的搜捕。仿真实验结果表明基于TOM匹配的搜捕方式可以有效的对抗箔条冲淡干扰,获得很高的捕获性能。

Micro-Tom番茄离体再生条件的选择

以Micro-Tom番茄子叶和下胚轴为外植体进行离体组织培养,研究了不同激素及浓度对愈伤组织诱导、不定芽分化及生根的影响。结果表明:培养基组成为MS+2.0mg/LZR+0.5mg/LIAA+3%蔗糖最有利于愈伤组织和不定芽的形成;诱导不定芽生根的理想培养基为1/2MS+1.5%蔗糖+0.2mg/LIBA。

英式歇后语Tom Swifty的认知语用解读

Tom Swifty是英语中在语义建构上与汉语歇后语相类似,并且在双关幽默方面能与汉语歇后语相媲美的一种语言形式。Tom Swifty的解读涉及到复杂的幕后认知。认知主义的哲学观认为语言意义存在于一种复杂的认知活动中,而概念整合理论能从认知的角度很好地将Tom Swifty幽默效果的获取过程动态地呈现出来。该研究不但可以丰富概念整合理论的解释力,而且有助于我们对Tom Swifty的理解。