CD34^(+)CD38^(-)subpopulation without CD123 and CD44 is responsible for LSC and correlated with imbalance of immune cell subsets in AML

Acute myeloid leukemia(AML)is regarded as a stem cell disease.However,no one unique marker is expressed on leukemia stem cells(LSC)but not on leukemic blasts nor normal hematopoietic stem cells(HSC).CD34^(+)CD38^(-)with or without CD123 or CD44 subpopulations are immunophenotypically defined as putative LSC fractions in AML.Nevertheless,markers that can be effectively and simply held responsible for the intrinsical heterogeneity of LSC is still unclear.In the present study,we examined the frequency of three different LSC subtypes(CD34^(+)CD38^(-),CD34^(+)CD38^(-)CD123^(+),CD34^(+)CD38^(-)CD44^(+))in AML at diagnosis.We then validated their prognostic significance on the relevance of spectral features for diagnostic stratification,immune status,induction therapy response,treatment effect maintenance,and long^(-)term survival.In our findings,high proportions of the above three different LSC subtypes were all significantly characterized with low complete remission(CR)rate,high relapse/refractory rate,poor overall survival(OS),frequent FLT3^(-)ITD mutation,the high level of regulatory T cells(Treg)and monocytic myeloid^(-)derived suppressor cells(M^(-)MDSC).However,there was no significant statistical difference in all kinds of other clinical performance among the three different LSC groups.It was demonstrated that CD34^(+)CD38^(-)subpopulation without CD123 and CD44 might be held responsible for LSC and correlated with an imbalance of immune cell subsets in AML.

STUDIES ON LYMPHOCYTE SUBSETS, HUMORAL IMMUNITY AND THEIR RELATIONSHIP WITH SELENIUM IN PATIENTS WITH KASHIN-BECK DISEASE

Objective To study the humoral immunity status and distribution pattern of lymphocyte subgroups of peripheral blood mononuclear cell (PBMC) in patients with Kashin-Beck Disease (KBD), and their relationship with erythrocyte selenium. Methods 23 X-ray diagnosed patients, 22 age- and sex- matched healthy children in KBD affected area (KAA), and 25 in KBD non-affected area (KNAA) were randomly selected. Immunohistochemistry with monoclonal antibodies anti-CD4, anti-CD8, anti-CD20 was conducted to analyze the lymphocyte subsets. Serum IgM, IgA, IgG, Complement C3 and C4 were assayed using rate nephelometry (Array 360 System, USA). The contents of erythrocyte selenium was determined by 2,3-diaminonaphthalene fluorescence assay. Results CD4+ and CD8+ cells percentage in PBMCs and serum IgA were significantly lower in KAA than those in KNAA(P< 0.05). CD20+ percentage in KAA displayed a decreasing trend compared to KNAA, although not statistically significantly. No statistical differences were found in CD4/CD8 ratio, serum IgG, IgM, C3 and C4 levels. Erythrocyte selenium level in KAA still showed a pronounced decrease compared to that in KNAA. Correlation analysis showed that erythrocyte selenium contents had a strong association with the CD4 cell percentage (r= 0.625, P< 0.05), as well as serum IgA (r= 0.462, P< 0.05). In addition, a moderate correlation between the serum IgA and CD4+ percentage (r= 0.130, P> 0.05) was found. Conclusion These results suggested that children in KAA had a comparably low cellular immunity level and their humoral immunity status was also in a state of moderate immune suppression. Of this immune disorder in Kashin-Beck disease patients, selenium deficiency probably played a critical role via affecting the distribution pattern of peripheral blood lymphocyte. Selenium-deficiency and immune impairment maybe both have something to do with the cause-effect chain of KBD.

Immunology of Kaschin-Beck Disease:Studies on lymphocyte subsets, humoral immunity and their relationship with selenium

Objective To study the humoral immunity status and distribution pattern of lymphocyte subgroups of peripheral blood mononuclear cell (PBMC) in patients with Kaschin-Beck Disease (KBD), and their relationship with erythrocyte selenium.Methods 23 X-ray diagnosed patients, 22 age- and sex- matched healthy children in KBD affected area (KAA), And 25 in KBD non-affected area (KNAA) were randomly selected. Immunohistochemistry with monoclonal antibodies anti-CD4, anti-CD8, anti-CD20 was conducted to analyze the lymphocyte subsets. Serum IgM, IgA, IgG, Complement C3 and C4 were assayed using rate nephelometry (Array 360 System, USA). The contents of erythrocyte selenium was determined by 2,3-diaminonaphthalene fluorescence assay. Results CD4+ and CD8+ cells percentage in PBMCs and serum IgA were significantly lower in KAA than those in KNAA(P<0.05). CD20+ percentage in KAA displayed a decreasing trend compared to KNAA, although not statistically significantly. No statistical differences were found in CD4/CD8 ratio, serum IgG, IgM, C3 and C4 levels. Erythrocyte selenium level in KAA still showed a pronounced decrease compared to that in KNAA. Correlation analysis showed that erythrocyte selenium contents had a strong association with the CD4 cell percentage (r=0.625,P<0.05), and also a close relationship with serum IgA (r=0.462,P<0.05). In addition, we detected a moderate correlation between the serum IgA and CD4+ percentage (r=0.130, P>0.05).Conclusion Taken together, our results suggested that children in KAA had a comparably low cellular immunity level manifested by the marked depression of CD4 and CD8 cells percentage, and their humoral immunity status was also in a state of moderate immune suppression. Of this immune disorder in KBD patients, selenium deficiency probably played a critical role via affecting the distribution pattern of peripheral blood lymphocyte. Selenium-deficiency and immune impairment maybe both have something to do with the cause-effect chain of KBD.

New immune multiobjective optimization algorithm and its application in boiler combustion optimization

In order to meet the requirements of combustion optimization for saving energy and reducing pollutant emission simultaneously,an immune cell subsets based multiobjective optimization algorithm（ICSMOA）is proposed.In the ICSMOA,the subset division operator and the immunological tolerance operation are defined.Preference can be easily addressed by using the subset division operator,and the distribution of the solutions can be guaranteed by the immunological tolerance operation.Using the ICSMOA,a group of Pareto optimal solutions can be obtained.However,by the traditional weighting method（WM）,only one solution can be obtained and it cannot be judged as Pareto optimal or not.In contrast to the solutions obtained by the repeatedly performed WM,the simulation results show that most solutions obtained by the ICSMOA are better than the solutions obtained by the WM.In addition,the Pareto front obtained by the ICSMOA is not as uniform as most classical multiobjective optimization algorithms.More optimal solutions which meet the preference set by the decision-maker can be obtained and they are very useful for industrial application.

Changes in T lymphocyte subsets after severe traumatic brain injury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.

Effect of Early Intensive Insulin Therapy on Immune Function of Aged Patients with Severe Trauma

This study examined the effect of intensive insulin therapy on immune function and inflammatory factors at the early phase after severe trauma. At day 1, 3, 5, 7 after admission, subsets of CD4＋ helper T lymphocytes （Th1/Th2） and human leukocyte antigen （HLA）-DR expression on CD14＋ monocytes were flow cytometrically measured. Levels of cytokines, including tumor necrosis factor-alpha （TNF-α）, interleukin-6 （IL-6）, interleukin-10 （IL-10） and other immunity markers, such as IgA, IgG, IgM, C3, C4 and C reaction protein （CRP） were examined in two groups. The results showed that TNF-α, IL-6 and CRP levels in the intensive insulin therapy group were significantly lower than those in the conventional therapy group, whereas IL-10 levels were substantially increased after intensive insulin therapy. C3 level at day 3, 5, 7 and C4 levels at day 5, 7 were lower in the intensive therapy group than in the conventional therapy group. Th1/Th2 ratios decreased gradually over time in both groups, and were much lower at day 3, 5, 7 in intensive therapy group. There were significant differences among day 3 to day 7 after admission in HLA-DR expression in CD14＋ monocytes. It was concluded that the intensive insulin therapy could decrease pro-inflammatory cytokines and increase anti-inflammatory cytokines in the elderly suffering from severe trauma, at the same time, with complement recovery being delayed. Moreover, intensive insulin therapy promoted immune suppression and, therefore, measures need be taken to address the issue.

Effect of liniment levamisole on cellular immune functions of patients with chronic hepatitis B

AIM: To explore the effects of liniment levamisole on cellular immune functions of patients with chronic hepatitis B. METHODS: The levels of T lymphocyte subsets and mlL-2R in peripheral blood mononuclear cells (PBMCs) were measured by biotin-streptavidin (BSA) technique in patients with chronic hepatitis B before and after the treatment with liniment levamisole. RESULTS: After one course of treatment with liniment levamisole, the levels of CD3+, CD4+, and the ratio of CD4+/CD8+ increased as compared to those before the treatment but the level of CD8+ decreased. The total expression level of mIL-2R in PBMCs increased before and after the treatment with liniment levamisole. CONCLUSION: Liniment levamisole may reinforce cellular immune functions of patients with chronic hepatitis B.

Changes of Some Immune Indexes after Resection of Liver Cancer and Their Relationship with Tumor Recurrence

In the present study, T lymphocyte subsets, NK and ADCC activity, and lymphocytetransformation rate (LT) were investigated by flow cytornetry and micro- LDH relcasing test in 67paticnts with hepatocellular carcinoma(HCC) and the relationship between changes of host immuni-ty after operation and tumor (?) were, further explored by follow-up study and generalanalysis. The results indicated that host immunosuppression became more severe in two weeks af-ter operation and then, gradually relurned to the level before opcration—"postoperativeimmunosuppressive period". Recovery time of postoperative immunosuppressive period andimmunc function condition of host after operation were closcly correlated with HCC recurrence rate.After operation, intensive immunologic examination can provide a valuable reference for finding highrisk population with turnor rccurrence and perfonning an effective postoperative immunotherapyagainst tumor rocurrence at (?)stage.

Evaluation of humoral immunity and peripheral blood T lymphocyte subset levels after Mycoplasma pneumoniae infection

Objective:To evaluate humoral immunity and peripheral blood T lymphocyte subset levels after Mycoplasma pneumoniae infection. Methods:118 cases of children with Mycoplasma pneumoniae infection were selected as observation group, and 98 cases of healthy children receiving medical examination in our hospital during the same period were selected as control group. The differences in humoral immunity, T lymphocyte subsets, anti-inflammatory and pro-inflammatory factors, Th1/Th2 balance state, etc were compared between two groups of children. Results:Serum IgA level of observation group was lower than that of control group (P<0.05), and IgM level was higher than that of control group (P<0.05). Peripheral blood CD3+and CD4+T lymphocyte levels as well as CD4+/CD8+ratio were lower than those of control group (P<0.05), while CD8+T lymphocyte level was higher than that of control group (P<0.05). Serum pro-inflammatory factors IL-8 and TNF-αlevels were higher than those of control group (P<0.05), while anti-inflammatory factors IL-10 and IL-13 levels were lower than those of control group (P<0.05). Serum IL-4, IFN-γand IL-4/IFN-γlevels were higher than those of control group (P<0.05). Conclusions:Humoral immune and cellular immune suppression, high inflammatory state and so on after Mycoplasma pneumoniae infection are the important mechanisms leading to disease progression.

Association of T lymphocyte subset counts with the clinical features of colorectal cancer

Background:Colorectal cancer(CRC)is a common gastrointestinal malignancy.The T lymphocyte subsets are important in the develop-ment,invasion and metastasis of tumors,including CRC.Nevertheless,limited research has explored the relationship between T cell subpopu-lations and the clinical characteristics of CRC.This study compared the T lymphocyte subsets in patients with CRC and healthy individuals,and assessed the relationship between these values and clinical characteristics.Methods:Peripheral blood was collected from 100 patients with CRC and 54 healthy individuals.The numbers of CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocytes,NK cells,and the CD4^(+)T/CD8^(+)T ratio in peripheral blood were measured using flow cytometry,and were compared between CRC patients and healthy individuals.Spearman's correlation analysis was performed to investigate the relationship between the T lymphocyte subsets in patients diagnosed with CRC and the levels of carcinoembryonic antigen(CEA)and thymidine kinase 1(TK1).Receiver operating characteristic(ROC)curves were utilized to evaluate the potential utility of the T lymphocyte counts in predicting lymph node metastasis,vas-cular infiltration,and high Ki-67 expression.Results:The CRC patients had lower counts of CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocytes compared to the healthy population(P<0.05).However,no significant differences were observed in the CD4^(+)/CD8^(+)ratio or NK cells(P>0.05).Notably,the CD3^(+)T,CD4^(+)T,and CD8^(+)T lym-phocyte counts were higher in patients with stageⅠ-Ⅱdisease,no lymph node metastasis,no vascular invasion,and low Ki-67 expression than in those with stageⅢ,lymph node metastasis,vascular invasion,and high Ki-67 expression(P<0.05).There was a negative association be-tween the CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts and CEA and TK1 levels in patients with CRC.The ROC curves demonstrated that CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts had significant predictive value for lymph node metastasis,vascular infiltration,and high Ki-67 expression.Conclusions:The peripheral blood CD3^(+)T,CD4^(+)T,and CD8^(+)T lymphocyte counts are related to the clinical traits of patients with CRC and can predict the prognosis of the disease.

活动性肺结核合并糖尿病患者血糖水平与树突状细胞亚群及T细胞亚群的相关性研究

目的研究活动性肺结核合并糖尿病患者血糖水平与树突状细胞(DCs)亚群及T细胞亚群的相关性。方法选取2020年1月至2022年1月惠州市中心人民医院接受诊治的200例患者为研究对象,按照病情分为单纯活动性肺结核患者(对照组)和活动性肺结核合并糖尿病患者(研究组),每组各100例。按照患者糖化血红蛋白(HbA1c)水平将研究组患者分为轻度高血糖组(10 mmol/L≤HbA1c<13.9 mmol/L)45例、中度高血糖组(13.9 mmol/L≤HbA1c<22 mmol/L)33例、重度高血糖组(HbA1c≥22 mmol/L)22例。比较每组患者血糖指标、DCs亚群、T细胞亚群、细胞因子水平,并分析不同血糖程度与DCs、T细胞亚群的相关性。结果四组患者各血糖指标比较为对照组<轻度高血糖组<中度高血糖组<重度高血糖组(P<0.05);DCs亚群(cDC1、cDC2、pDC)及T细胞亚群(CD4^(+)、CD8^(+)、CD3^(+))分布值比较为对照组>轻度高血糖组>中度高血糖组>重度高血糖组(P<0.05);四组患者各炎性细胞因子水平比较为对照组<轻度高血糖组<中度高血糖组<重度高血糖组(P<0.05);外周血DCs亚群、T细胞亚群分布情况与血糖水平呈负相关(P<0.05)。结论针对活动性肺结核合并糖尿病患者,不同血糖程度患者炎性细胞因子水平、DCs亚群及T细胞亚群分布情况不同,且DCs亚群及T细胞亚群分布情况与患者血糖水平呈负相关。

楮芍凉血汤加减治疗寻常型银屑病的效果及对免疫功能、辅助性T淋巴细胞17相关因子的影响

目的观察楮芍凉血汤加减治疗寻常型银屑病的临床疗效和对免疫功能、辅助性T淋巴细胞17(Th17)相关因子的影响。方法选取2020年2月至2021年12月在本院接受治疗64例进行期PV患者,采用随机数字表法将患者分为对照组和观察组各32例。对照组患者接受常规西药治疗,观察组接受楮芍凉血汤加减治疗,治疗周期为6周。所有研究对象均检测外周血T淋巴细胞亚群、分离单个核细胞(PBMC)情况,分析PBMC中Thl7细胞相关因子白细胞介素17(IL-17)、IL-22、维甲酸相关孤儿受体γ(ROR-γ)mRNA、干扰素γ(INF-γ)mRNA相对表达量,并对比临床总疗效。结果治疗6周后,观察组总有效治疗30例(93.75%),对照组总有效治疗24例(75.00%),2组总疗效差异具有统计学意义(P<0.05)。治疗6周后,2组患者T淋巴细胞指标CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平均升高,CD8^(+)及Th17相关因子IL-17、IL-22、RORC mRNA、INF-γmRNA相对表达量均降低,组内不同时点和组间同时点比较差异均具有统计学意义(P<0.05)。结论楮芍凉血汤可有效治疗寻常型银屑病,且作用机制与T淋巴细胞亚群分布及Thl7细胞相关因子有关,可以提高机体免疫功能,以此减少不良症状,提高临床疗效。

巨型艾美耳球虫Th1类细胞因子刺激性分子EmARM-β对鸡PBMC和T细胞亚群免疫功能的影响

[目的]本文旨在探讨巨型艾美耳球虫Th1类细胞因子刺激性分子Armadillo/β-catenin样重复序列蛋白(EmARM-β)对鸡免疫细胞功能的影响。[方法]利用密度梯度离心法和免疫磁珠分选法分别分离出鸡外周血单个核细胞(peripheral blood mononuclear cell,PBMC)、CD8^(+)T细胞和CD4^(+)CD25^(-)T细胞,之后将EmARM-β重组蛋白(rEmARM-β)分别与上述细胞进行体外共孵育6 h,利用CCK-8试剂和qPCR法分别检测并分析其对上述细胞增殖能力及相关细胞因子分泌水平的影响。[结果]经免疫磁珠分选后,流式细胞术检测鸡CD8^(+)T细胞和CD4^(+)CD25^(-)T细胞的纯度分别为93.01%和88.88%。与对照组相比,rEmARM-β显著促进鸡PBMC的增殖能力,显著上调Th1类细胞因子(ifn-γ和il-2)和促炎性细胞因子(il-1β、il-6和il-17a)mRNA水平;显著促进CD8^(+)T细胞的增殖能力,显著上调其ifn-γ、il-2和tnf-α mRNA水平,显著上调穿孔素、fasl(Fas配体)和fas mRNA水平;能显著促进CD4^(+)CD25^(-)T细胞的增殖能力,显著上调其ifn-γ和il-2 mRNA水平,下调il-4和il-10 mRNA水平。[结论]EmARM-β可以有效促进鸡PBMC和T细胞亚群(CD8^(+)T细胞和CD4^(+)CD25^(-)T细胞)的增殖能力,提升其Th1类细胞因子和促炎性细胞因子的分泌水平,提示EmARM-β具有研发鸡球虫病新型疫苗候选抗原的潜力。

火针联合穴位注射治疗带状疱疹后遗神经痛的临床疗效研究

目的:通过观察火针联合穴位注射疗法干预带状疱疹后遗神经痛患者的临床疗效及对患者血清中相关神经递质、免疫细胞和炎症因子的影响,探讨火针联合穴位注射疗法的作用机制。方法:收集2022年1月-12月于甘肃省中医院针灸科就诊的带状疱疹后遗神经痛患者60例,将患者随机分为对照组和治疗组,每组30例,对照组给予火针单一疗法,治疗组采用火针联合穴位注射疗法。10 d为一个疗程,隔日1次,2个疗程后观察治疗前后两组患者临床症状,检测血清中相关神经递质血清神经肽Y、P物质、β-内啡肽、T淋巴细胞亚群(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))和炎症因子白细胞介素-6、白细胞介素-1β、白细胞介素-10、肿瘤坏死因子-α等指标。结果:与对照组治疗后比较,治疗组汉密尔顿焦虑量表(Hamilton Anxiety Scale,HAMA)评分、视觉模拟评分法(Visual Analogue Scale,VAS)评分、匹茨堡睡眠质量指数量表(Pittsburgh Sleep Quality Index,PSQI)评分明显降低,生活质量测定量表(Quality of Life-Brief Scale,QOL-BREF)评分明显增高(治疗组生理评分除外),差异具有统计学意义(P <0.05);治疗组血清相关神经递质神经肽Y、P物质明显降低,β-内啡肽明显增高,治疗组CD8^(+)水平明显降低,CD4^(+)、CD4^(+)/CD8^(+)水平明显增高,炎症因子白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α明显降低,白细胞介素-10明显增高,上述差异均具有统计学意义(P <0.05)。结论:火针联合穴位注射复合疗法可有效缓解患者焦虑情绪、减轻疼痛程度、改善睡眠状态及提高生活质量,临床疗效显著,优于火针单一疗法。其干预带状疱疹后遗神经痛患者临床疗效的作用机制可能与调控相关神经递质水平、炎症因子水平及调节免疫功能有关。

MicroRNA调节免疫性血小板减少症CD4^(+)T细胞亚群免疫功能的研究进展

MicroRNA(miRNA)是一种小分子非编码单链RNA,可广泛参与多种生物学过程,对调节机体的免疫功能具有重要作用。免疫性血小板减少症(ITP)是一种自身免疫性疾病,其发生和进展与miRNA介导CD4^(+)T细胞亚群的异常免疫调节密切相关。ITP患者miRNA的不同表达情况可影响CD4^(+)T细胞亚群的免疫功能,导致Th1/Th2、Th17/Treg的调节失衡和TFH的过度分化,引起细胞因子分泌异常。本文就ITP中miRNA介导的CD4^(+)T细胞亚群免疫功能失衡机制进行总结,以期为探索ITP的免疫机制和免疫治疗提供启发。

脐带间充质干细胞对干燥综合征患者外周血的免疫调控研究

目的:研究脐带间充质干细胞(UC-MSCs)对干燥综合征(SS)患者外周血的免疫调节作用。方法:取新生儿脐带组织分离并培养UC-MSCs,流式细胞术检测UC-MSCs的细胞表面标记;选取20例疾病活动期的SS患者,收集静脉血提取外周血单个核细胞(PBMC),将SS患者的PBMC与UC-MSCs共培养,CCK-8法检测细胞存活率,筛选最优共培养细胞比例进行后续实验;流式细胞术检测共培养后SS患者PBMC中淋巴细胞亚群的变化情况。结果:UC-MSCs可降低SS患者PBMC中的外周血CD3^(+)T细胞、CD3^(+)CD8^(+)T细胞以及CD19^(+)B细胞的比例,而CD16^(+)CD56^(+)NK细胞比例、CD3^(+)CD4^(+)T细胞比例、CD4^(+)/CD8^(+)无明显变化。结论:UC-MSCs可以调节SS患者的免疫功能,从而对SS有一定的治疗作用。

Dendritic cell subsets in cancer immunity and tumor antigen sensing

Dendritic cells(DCs)exhibit a specialized antigen-presenting function and play crucial roles in both innate and adaptive immune responses.Due to their ability to cross-present tumor cell-associated antigens to naïve T cells,DCs are instrumental in the generation of specific T-cell-mediated antitumor effector responses in the control of tumor growth and tumor cell dissemination.Within an immunosuppressive tumor microenvironment,DC antitumor functions can,however,be severely impaired.In this review,we focus on the mechanisms of DC capture and activation by tumor cell antigens and the role of the tumor microenvironment in shaping DC functions,taking advantage of recent studies showing the phenotype acquisition,transcriptional state and functional programs revealed by scRNA-seq analysis.The therapeutic potential of DC-mediated tumor antigen sensing in priming antitumor immunity is also discussed.

T细胞亚群在射血分数保留的心力衰竭中的研究进展

射血分数保留的心力衰竭(heart failure with preserved ejection fraction,HFpEF)是心血管领域急需解决的重大难题之一。系统性炎症、心肌纤维化及冠脉微循环功能障碍等被认为是HFpEF发生发展的核心病理生理机制。T细胞亚群作为适应性免疫应答的关键一环,可通过多种机制参与上述过程,进一步加重心肌结构、功能的损伤并最终导致HFpEF的发生发展。文章回顾了免疫与炎症在HFpEF中的作用,总结了不同T细胞亚群在HFpEF中的作用及特点,并尝试以T细胞亚群来探寻HFpEF的新型生物标志物及潜在的治疗靶点。

卡瑞利珠单抗联合仑伐替尼对晚期肝癌患者疗效及T淋巴细胞亚群的影响

目的 探究卡瑞利珠单抗联合仑伐替尼对晚期肝癌疗效及T淋巴细胞亚群的影响。方法 选取2021年8月至2022年6月陕西省核工业二一五医院收治86例晚期肝癌患者,通过随机数字表法分为观察组和对照组,每组43例。对照组给予仑伐替尼治疗,观察组在仑伐替尼的基础上增加了卡瑞利珠单抗。对比及分析两组患者临床疗效、T淋巴细胞亚群(包括CD3^(+)、CD4^(+)、CD8^(+)以及CD4^(+)/CD8^(+))及不良反应发生率。结果 观察组疾病控制率为(79.07%)高于对照组(53.49%)(χ^(2)=6.295,P<0.05),两组客观缓解率比较差异无统计学意义(55.81%vs 37.21%,χ^(2)=2.991,P>0.05);治疗后,观察组CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)[分别为(72.78±7.21)%、(47.34±6.21)%、(1.98±0.45)]高于对照组[分别为(63.45±6.88)%、(37.02±5.02)%、(1.41±0.32)](t=6.139、8.475、6.769,均P<0.05),观察组CD8^(+)低于对照组[(22.43±2.11)%vs(25.86±2.89)%,t=6.286,P<0.05];两组不良反应发生率比较差异无统计学意义(11.63%vs 23.26%,χ^(2)=2.019,P>0.05)。结论 卡瑞利珠单抗联合仑伐替尼治疗晚期肝癌的效果显著,有助于恢复T淋巴细胞亚群比例,改善免疫功能,安全性高。

基于关键免疫细胞亚群的胆管癌患者外周血早期诊断标志物的研究

目的基于关键免疫细胞亚群探究胆管癌患者外周血早期诊断标志物。方法于2021年5月至2023年5月选取在本院确诊为CCA的患者75例即为CCA组,同期在本院收治的75例胆管炎患者即为对照组。同期在本院体检健康的志愿者75例为健康组。采用流式细胞仪检测CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)水平;采用酶联免疫吸附试验(ELISA)法检测肿瘤坏死因子(TNF-α)、白细胞介素-2(IL-2)、γ干扰素(IFN-γ)水平。采用Pearson法分析CCA患者CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平与TNF-α、IL-2、IFN-γ水平的相关性;ROC曲线分析外周血免疫细胞亚群对CCA发生的诊断价值。多因素Logistic回归分析CCA发生的影响因素。结果与健康组相比,对照组、CCA组患者CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平显著升高;与对照组相比,CCA组患者CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平显著升高(P<0.05)。与健康组相比,对照组、CCA组患者IL-2、IFN-γ水平显著降低,TNF-α水平显著升高;与对照组相比,CCA组患者IL-2、IFN-γ水平显著降低,TNF-α水平显著升高(P<0.05)。相关性分析显示,CCA患者CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平与IL-2、IFN-γ呈负相关关系,与TNF-α呈正相关关系(P<0.05)。外周血免疫细胞亚群(CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)、TNF-α、IL-2、IFN-γ)联合检测对CCA发生诊断的AUC显著高于单一指标诊断的AUC的值(Z_(CD3^(+)vs联合)=4.424,P<0.001;Z_(CD4^(+)vs联合)=3.425,P=0.001;Z_(CD4^(+)/CD8^(+)vs联合)=4.502,P<0.001;Z_(TNF-αvs联合)=3.322,P<0.001;Z_(IL-2^(+)vs联合)=7.473,P=0.001;Z_(IFN-γ^(+)vs联合)=3.166,P=0.001)。多因素Logistic回归分析显示,CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)、IL-2、IFN-γ水平是影响CCA发生的影响因素(P<0.05)。结论CCA患者外周血免疫细胞亚群水平显著升高,联合检测能够提高对CCA发生的诊断效能。