Target Prediction of Xinyi San for Rhinitis Based on Network Pharmacology

Objective: To analyze the potential mechanism of Xinyi San in treating rhinitis through network pharmacology. Methods: In the database of Traditional Chinese Medicine Systems Pharmacology (TCMSP), chemical composition and potential targets of Xinyi San were got, and the target genes of rhinitis of Xinyi San were extracted from GeneCards databases. Then we constructed protein-protein interactions (PPI) network of target genes, and then analyzed the Key genes in GO analysis and KEGG analysis. Results: We got 97 components, 53 potential therapeutic targets, 1009 GO items and 92 pathways in our study. The main pathways included Lipid and atherosclerosis, Chemical carcinogenesis-receptor activation, PI3K-Akt signaling pathway, Human cytomegalovirus infection, Prostate cancer, etc. Conclusion: Xinyi San plays a role in treating rhinitis through multiple components, multiple targets and multiple pathways.

The Molecular Mechanism of Xinyi San for the Treatment of Senile Rhinitis Based on Network Pharmacology

Objective: To study the molecular mechanism of Xinyi San for the treatment of senile rhinitis by applying network pharmacological analysis technology. Methods: The effective components and corresponding targets of Xinyi San were collected by TCMSP. The targets of senile rhinitis were collected by the Genecards database. The potential target of Xinyi San in the treatment of senile rhinitis was obtained by Venn analysis. Cytoscape 3.7.2 the software constructs the relationship network model of “disease-single drug-active ingredient-action target”. Protein protein interaction (PPI) network was constructed by using a string database. R4.1.1 software was used for GO function enrichment analysis and KEGG pathway enrichment analysis. Results: In this study, we obtained 158 active ingredients, 40 potential therapeutic targets, 74 GO projects, and 99 pathways. Major pathways include Lipid and atherosclerosis, Chemical carcinogenesis-receptor activation, PI3K-Akt signaling pathway, AGE-RAGE signaling pathway in diabetic complications, Pathways of neurodegeneration-multiple diseases, etc. Conclusion: Xinyi San has the characteristics of multi-component, multi-target, and multi-channel in the treatment of senile rhinitis. This study provides a basis for the in-depth study of Xinyi San.

Study on the Specific GC Chromatograms of Volatile Oil from Xinyi Biyan Pills

[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manufacturers,and further improve the quality control methods.[Methods]The volatile oil in samples was extracted and tested by gas chromatography to collect chromatograms,which were analyze and evaluated by the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine.[Results]Nineteen common peaks were calibrated in the characteristic chromatograms;and the characteristic chromatograms of samples produced by different manufacturers were obviously different.[Conclusions]Controlling the volatile components in Xinyi Biyan Pills by the established characteristic chromatograms of GC is accurate and feasible,and can be used as a quality control method for Xinyi Biyan Pills.

Target Prediction of Xinyi San for Nasal Polyposis Based on Network Pharmacology

Objective: To analyze the potential mechanism of Xinyi San in treating nasal polyposis through network pharmacology. Methods: We Screened the active components and targets of Xinyi San by TCMSP database, and disease targets through GeneCards database. We constructed “disease-single drug-component-target” network through software Cytoscape 3.7.2 and constructed PPI network through STRING database. GO function and KEGG pathway enrichment were analyzed to predict its mechanism. Results: We got 162 components, 69 therapeutic targets, 88 GO items and 135 pathways. The main pathways include Lipid and atherosclerosis, Chemical carcinogenesis-receptor activation, Kaposi sarcoma-associated herpesvirus infection, Hepatitis B, Human cytomegalovirus infection, etc. Conclusion: This study preliminarily revealed the active components, targets and pathways of Xinyi San in treating nasal polyposis.

Aqueous Chemistry of Typical Geothermal Springs with Deep Faults in Xinyi and Fengshun in Guangdong Province,China

This paper investigated aqueous chemistry for two geothermal spring groups responsive and sensitive to flow fluctuations induced by earthquakes. Quake monitorings are favored for their being in residential areas with well-preserved natural flow systems in Xinyi City＇s Xijiang Hot Springs and Fengshun County＇s Shihu Hot Spring. The hot springs are typical in temperatures and flow rates in southern China＇s Guangdong Province. Physical and chemical conditions deep down in the heat sources are important constraints on earthquake, fluid flow, reactive solute transport and heat transfer, but remain challenging to address via field observations and numerical experiments. In this paper, we made daily and annual observations on flow rates, temperature, and/or aqueous chemistry. We employed strontium isotopes as tracers for the water sources, equilibrium phase diagram for K-feldspar and albite stability, and Na-K-Mg diagram for heat reservoir temperatures. The abundant sulfite content in Xi- jiang Hot Springs is discussed. Our main finding are that the deep fault springs are characterized by low reduction-oxidation potential at around -200- -150 mV and relatively large daily flow variations. The resuits provide scientific background features on the field sites regarding earthquake monitoring and predictions and geothermal reservoir.

Effects of Composite Xinyi Aerosol (复方辛夷雾化剂) on Asthma Related Cytokines in Serum and Sputum of Patients with Bronchial Asthma

Objective: To observe the therapeutic effect of composite Xinyi ae rosol (复方辛夷雾化剂, XYA) in treating asthma and its influence on asthma relat ed cytokines in serum and sputum of patients with mild or moderate bronchial ast hma.Methods: Sixty patients were divided randomly into the treated gr oup and the control group, 30 in each group. They were treated with XYA and Salb utamol aerosol (SA) respectively by spray inhalation once a day for seven days a s one therapeutic course to observe the antiasthmatic effect of the treatment. Changes of lung function and the levels of tumor necrosis factor α (TNF-α) , interleukine-2 receptor (IL-2R) and interleukine-8 (IL-8) in serum and sputum were observed before and after treatment using enzyme linked immunos orbent assay (ELISA). Levels of TNF-α, IL-2R andIL-8 in the sputum of part of the patients in the treatment group were also observed.Results: In the treated group, 6 patients had the asthma clinica lly controlled after treatment, 12 were markedly alleviated, 9 alleviated and 3 remained unchanged, while in the control group, the respective numbers were 4, 6 , 15 and 5. The controlled-markedly effective rate of XYA was 60.0%, which wa s significantly better than that of SA (33.3%, P <0.05). The one second forc ed expiratory volume percent (FEV1%) and peek expiratory flow rate (PEFR) improv ed significantly and the levels of TNF-α, IL-2R and IL-8 in serum decr eased noticeably in both groups after treatment ( P <0.05 or P <0.01), but the improvement in the treated group was more significant as compared with tho se in the control group ( P <0.05). The levels of TNF-α, IL-2R and IL -8 in sputum considerably lowered also after XYA treatment ( P <0.01).Conclusion: XYA could regulate the related cytokines in patient s with mild or moderate bronchial asthma, and reduce the releasing of inflammato ry factors, which is consistent with the improvement of lung functions, so as to ease the airway inflammation chiefly exhibited as cell infiltration in bron chi.

辛夷的法象药理及其临床意义

“药象”是中药在自然生长和形成过程中产生的固有性质,也是形成药性药效的基础。辛夷独特的药象特征一直是古人细致观察与重点关注的内容。辛夷“必至四序并经,乃刳苞以出”的物候之象,可谓花木之独一份,也奠定了其独特的药性特点;辛夷形若毛桃,苞片坚韧而密被绒毛的药材之象,也是探讨其“以形治形”论治痼积癌肿的法象特征。经典本草古籍中辛夷广泛用于治疗风邪郁滞、经脉不通等诸多病证的作用机制皆与其药象特点密切相关。虽然后世受“舍鼻塞、鼻渊之症,无他用”之论所囿,但从其“法象药理”而言,辛夷不仅可轻宣升散,又善于内入脏腑以宣达阴伏之邪,深入巢窟以驱逐结聚之滞,可广泛应用于气血郁滞、沉寒痼冷、瘀毒结聚等病证。而这些功效主治的拓展,正是辛夷“象性合参”的意义所在。

苏鲁造山带新沂地区新元古代花岗片麻岩成因及对Rodinia超大陆裂解的响应

新沂地区花岗片麻岩位于苏鲁造山带的西缘。本文通过新沂地区花岗片麻岩岩相学、岩石地球化学、锆石U-Pb同位素年代学等方面的研究,探讨其成因与构造环境,以揭示Rodinia超大陆裂解事件在该地区的反响。研究认为:研究区花岗片麻岩属准铝质-弱过铝质A型花岗岩,具有高SiO_(2)、富碱、贫CaO、低Al_(2)O_(3)质量分数的特征,以及右倾海鸥型稀土元素配分模式,富集Rb、Zr、Hf等元素,严重亏损Sr、Eu、Nb、Ta等元素,形成年龄为746.0~742.5 Ma。新沂地区花岗片麻岩是来自下地壳物质为主、少量幔源物质的部分熔融,在岩浆演化过程中经历了以钾长石和斜长石为主的分离结晶,而后经过超高压变质作用最终形成。研究区花岗片麻岩形成于新元古代后碰撞伸展环境,是Rodinia超大陆裂解事件在苏鲁造山带新沂地区的最初响应。

江苏省新沂河流域短历时降水极值统计规律研究

为揭示江苏省新沂河流域短历时降水极值统计规律,以研究区内的10个雨量站1981—2020年历史实测资料为依据,以年最大1 h、3 h、6 h、12 h和24 h降水极值为研究对象,采用Mann-Kendall检验和Morlet小波分析,开展了新沂河流域不同历时面雨量极值趋势性、跳跃性和周期性分析。研究短历时降水极值的统计规律可为地区工程防洪设计与规划提供参考。

辛荑清肺饮加减联合复方木芙蓉涂鼻软膏治疗儿童慢性鼻炎临床观察

目的探讨辛荑清肺饮加减联合复方木芙蓉涂鼻软膏治疗慢性鼻炎的临床疗效。方法选取医院2021年1月—2021年12月收治的110例慢性鼻炎患者随机均分为观察组(应用复方木芙蓉涂鼻软膏联合辛荑清肺饮)和对照组(应用辛荑清肺饮)各55例。比较两组临床疗效、症状积分、鼻通气功能、用药时间、复发率、生活质量、鼻内镜评分、免疫功能和不良反应。结果治疗后,观察组总有效率高于对照组(P<0.05);观察组症状积分低于对照组(P<0.05);观察组鼻通气功能优于对照组(P<0.05);观察组用药时间少于对照组(P<0.05);观察组复发率低于对照组(P<0.05);观察组生活质量低于对照组(P<0.05)。观察组患者鼻内镜评分低于对照组(P<0.05);观察组免疫功能高于对照组(P<0.05);观察组不良反应低于对照组(P<0.05)。结论辛荑清肺饮加减联合复方木芙蓉涂鼻软膏能够提高患者临床疗效,改善症状与鼻通气功能,减少持续用药时间与复发率,提升患者免疫功能。

江苏新沂高流地区土壤地球化学特征及找矿预测

在总结江苏新沂高流地区地质特征的基础上,结合区内1∶10000土壤地球化学调查工作,系统研究该区元素含量及组合特征,并进行找矿预测。异常结果显示该区Au、Ag、Cu、Mo、Pb、Zn、As、Sn等单元素地球化学异常明显,其中Au、Ag、Mo 3个元素的异常强度和分带性均好,其形态分布受NNE向构造破碎和碱长花岗岩体等因素制约。在研究区共圈定了14处综合异常,通过异常评价,位于同一断裂带附近的3处异常成矿地质条件优越;对综合异常开展地球化学剖面查证工作,结果显示其中1处Au、Cu等元素峰值均位于NNE向断裂带中心位置。结合该区成矿地质条件和土壤地球化学异常特征,初步总结了研究区金、多金属矿找矿标志,为该区进一步找矿提供间接依据。

紧密连接蛋白介导的十三味辛夷滴鼻剂对小鼠鼻腔黏膜修复机制探究

目的 探讨十三味辛夷滴鼻剂对鼻腔黏膜损伤小鼠的修复作用及其可能的干预机制。方法 制备小鼠鼻腔黏膜热损伤模型,随机分为空白对照组、模型对照组、滴通鼻炎水阳性对照组[1μl/(只·次·侧)]、十三味辛夷滴鼻剂高剂量[5μl/(只·次·侧)]、中剂量[2.5μl/(只·次·侧)]、低剂量组[1.25μl/(只·次·侧)],每组各10只,雌雄各半。给药7 d后,分级评价鼻黏膜红肿程度,苏木素-伊红(HE)染色观察鼻黏膜组织病理结构改变,免疫组化分析鼻黏膜上皮ZO-1、Claudin-1、Occludin表达水平。结果 给药7 d后,模型对照组红肿程度评分显著高于空白对照组(P <0.05),滴通鼻炎水阳性对照组、十三味辛夷滴鼻剂高、中、低剂量组均低于模型对照组;HE染色显示,十三味辛夷滴鼻剂各组随剂量增高,黏膜层逐渐变薄、黏膜结构趋于整齐;与模型对照组比较,十三味辛夷滴鼻剂高剂量组ZO-1、Claudin-1、Occludin蛋白表达量显著提高(P <0.05)。结论 十三味辛夷滴鼻剂可能通过上调鼻黏膜上皮紧密连接蛋白ZO-1、Claudin-1、Occludin的表达,修复小鼠鼻腔黏膜损伤。

田心义教授基于托毒补虚理论治疗创伤后慢性骨髓炎经验

慢性骨髓炎是多因创伤后出现急性骨髓炎治疗不恰当或延期治疗引起的一种炎症性疾病,属于中医“附骨疽”范畴。此病在临床治疗上存在治疗周期长、不易根治、抗生素耐药、反复手术等问题,通过中医药辨证论治治疗本病具有明显的疗效,文章总结了田心义教授治疗创伤后慢性骨髓炎的临证经验。田心义教授认为,本病多属于虚实夹杂证,外来损伤致皮肤肌肉受损,卫外之能丧失,邪毒注骨,进一步导致患者脏腑功能失调,气血生化无源,病迁延难愈;治疗中托毒补虚,内外兼施,汤膏并用,以恢复患者健康,提高患者生活自理能力。

基于网络药理学和分子对接技术探究经典名方辛夷散治疗过敏性鼻炎的潜在作用机制

目的利用网络药理学和分子对接技术研究古代经典名方辛夷散用于过敏性鼻炎防治的潜在作用机制。方法通过TCMSP数据库检索经典名方辛夷散的活性成分并确定作用靶点,通过GeneCards Database、Online Mendelian Inheritance in Man数据库查询与过敏性鼻炎有关的分子靶点,获取两个数据库交集靶点基因。通过Search tool for the retrival of interacting genes/proteins数据库构建上述交集靶点的蛋白质相互作用网络,并导入Cytoscape 3.7.1软件中将分析结果可视化,进一步根据拓扑学数据筛选关键靶点。利用Metascape数据库对交集靶点进行GO和KEGG富集分析。运用AutoDockTools1.5.6软件将活性化合物与核心靶点蛋白进行分子对接。结果筛选得到辛夷散活性化合物184个,相关靶点224个,辛夷散与过敏性鼻炎共同的靶点104个。利用拓扑学筛选得到17个关键靶点,主要包括IL-6、TNF、AKT1、IL1B、VEGFA等。GO功能富集分析表明辛夷散治疗过敏性鼻炎可能涉及脂多糖的反应、细胞对有机环状化合物反应、氧化应激反应等1780条生物过程。KEGG通路结果显示主要参与IL-17信号通路、HIF-1信号通路、糖尿病并发症中的AGE-RAGE信号通路、癌症通路等。分子对接结果显示方中主要活性成分山奈酚、维斯体素、柚皮素、β-谷甾醇、芒柄花黄素等与IL-6、TNF、AKT1、IL1B、VEGFA等均能实现自发结合。结论经典名方辛夷散可能通过多成分、多靶点及多种通路治疗过敏性鼻炎,为深入研究辛夷散的作用机制提供了理论参考。

不稳定煤层多场联合勘探综合解译技术研究与应用--以河南新义煤矿为例

地质条件复杂的工作面由于勘探条件较差或者存在多种隐蔽致灾地质因素干扰,导致单一的物探手段难以准确辨识不良地质体,需要采用多种物探方法进行联合勘探,相互补充印证,以解决物探多解性问题。针对新义煤矿11080工作面揭露的大范围薄化带,采用地震槽波速度场、质量场、能量场以及无线电波透视法联合勘探,对不良地质体进行多维度、多方面的探查,开展多场耦合综合解译,圈出了工作面煤层薄化带和开采关注区。结果显示,多场联合勘探综合解译技术能够对复杂地质条件下极不稳定煤层进行有效探查,能够准确判断不良地质体的相关属性,为工作面安全高效生产提供依据,该方法能够在类似地质条件工作面推广。

广东信宜市托盘洞锡矿地质特征及找矿远景分析

本文以信宜市托盘洞锡矿区为背景,分析了矿区地质特征、土壤异常特征和矿体特征,并对找矿远景进行了评估。未来的工作应以深部矿床为重点,以期发现中大型锡多金属矿床。

广东信宜市三叉坳金多金属矿找矿前景分析

基于广东信宜三叉坳金多金属矿区地质成矿背景、地质特征、土壤测量异常数据以及矿体化特征分析。显示金多金属矿体存在迹象。以及多条含金黄铁矿化硅化破碎带的出露,呈现出较好的找矿潜力和前景。

超临界CO_2流萃取及与水蒸气蒸馏萃取辛夷挥发油的化学组分分析

采用超临界ＣＯ２ 流萃取辛夷挥发油，对在不同条件下萃取的辛夷挥发油，用色谱－ 质谱（ＧＣ－ ＭＳ） 联用方法进行了化学组分的测定和分析，并与水蒸气蒸馏法所得之挥发油化学组分相比较，发现超临界ＣＯ２ 流萃取的挥发油香气、品质和产率均优于蒸馏法所得。

郯庐断裂带中段嶂山东侧断裂的活动特征

目前对于郯庐断裂带新沂—泗洪段的研究相对薄弱,通过对该段北侧构造地貌条件较好的嶂山段开展研究,充实了该段的晚第四纪活动证据与古地震事件序列。嶂山段总体位于宿迁市晓店镇至井头乡一带,长约7km。构造地貌研究显示嶂山段晚第四纪活动具有逆冲右旋的运动性质。古地震探槽研究揭示:1)郯庐断裂在该段具有分期活动特征,上新世期间为拉张正断,第四纪以来表现为逆冲;2)1次古地震发生在15.12~11.82ka BP;3)断裂最新活动年代可能为3500a BP左右。综合前人的研究成果,目前郯庐断裂带新沂—泗洪段已揭露的古地震事件年代序列可归纳为:9.6×10~4a以前,晚更新世早、中期,15.12~11.82ka BP,(11.76±0.05)^(10.53±0.05)ka BP,(10.15±0.05)^(8.16±0.05)ka BP,4960~3510a BP。