In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yuunan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5...In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yuunan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5 bioactive glass powder （16% weight） with Vaseline and different weight percentages of Yurman baiyao. Full-thickness defect wounds were created on the back of 130 SD rats and were randomly divided into 8 groups. The wound healing rates were calculated at 4, 7, 10, 14 and 21 days after surgery. The samples were harvested for further observations. Considering the wound closure rate, group 6 （with 5% Yuunan baiyao） has better wound healing performance than other diabetic groups. The lower inflammatory response was observed by gross observation and confirmed by the results of H＆E staining and TEM observation. Besides, the proliferation of fibroblasts, the formation of granulation tissue, as well as the vascularization, were improved in group 6 compared to other diabetic groups. All results suggest that bioactive glass and Yunnan baiyao ointments can accelerate the recovery of diabetes-impaired skin wounds, and comparing to other diabetic groups, group 6 （with 5% Yunnan baiyao） has better healing effect.展开更多
Objective: To observe the clinical efficacy of Yunnan Baiyao (云南白药, YNBY) combined with antibiotics in treating Helicobacter pylori (HP) related duodenal ulcer (DU). Methods: Eighty two HP positive DU patients ...Objective: To observe the clinical efficacy of Yunnan Baiyao (云南白药, YNBY) combined with antibiotics in treating Helicobacter pylori (HP) related duodenal ulcer (DU). Methods: Eighty two HP positive DU patients were randomly divided into two groups: Treated group (44 cases) used YNBY plus amoxicillin and metronidazole; control group (38 cases) used de nol plus amoxicillin and metronidazole). Results: After a 2 week treatment course, the effect of epigastric pain relief was 68.3% in treated group, and 44.4% in control group, the difference between them was significant ( P <0.05). Marked effect in acid regurgitation and belching relief was observed in both groups. The rate of DU healing in treated and control group was respectively 88.6% and 89.5%; the clearance of HP 84.1% and 89.5%; and the side effects 4.5% and 13.5%, with insignificant difference between these two groups. Conclusion: YNBY combined with antibiotics in treating DU, and clearance of HP is an economic, safe and effective therapy.展开更多
背景:活髓切断术现作为永久保存活髓的治疗方法,术中选择合适的盖髓材料可在较大程度上增强其临床疗效并扩大其应用范围。目的:探讨云南白药、iRoot BP Plus及其联合应用对炎症状态下牙髓干细胞矿化的影响。方法:采用组织块法培养人原...背景:活髓切断术现作为永久保存活髓的治疗方法,术中选择合适的盖髓材料可在较大程度上增强其临床疗效并扩大其应用范围。目的:探讨云南白药、iRoot BP Plus及其联合应用对炎症状态下牙髓干细胞矿化的影响。方法:采用组织块法培养人原代牙髓干细胞,MTT法测定云南白药、iRoot BP Plus对牙髓干细胞增殖的影响,确定最佳干预质量浓度。将第3-6代牙髓干细胞分为5组:正常对照组、炎症对照组、云南白药组、iRoot BP Plus组、云南白药+iRoot BP Plus组,后4组使用1μg/mL脂多糖诱导2 h进行细胞造模,然后分别采用75μg/mL云南白药、100μg/mL iRoot BP Plus及两者联用进行刺激,采用碱性磷酸酶染色法、茜素红S染色法、Real time-PCR法、Western blot法检测药物对细胞矿化的影响。结果与结论:①与炎症对照组比较,云南白药、iRoot BP Plus及其联合应用处理后抑制了肿瘤坏死因子αmRNA的表达(P<0.001),云南白药+iRoot BP Plus组肿瘤坏死因子αmRNA的表达明显低于云南白药组、iRoot BP Plus组(P<0.001);②与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后矿化结节数量、碱性磷酸酶活性显著增加,云南白药+iRoot BP Plus组矿化结节量、碱性磷酸酶活性高于云南白药组、iRoot BP Plus组(P<0.001);③与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后DSPP、IBSP、MEPE mRNA的表达增加(P<0.001),云南白药+iRoot BP Plus组DSPP、IBSP、MEPE mRNA的表达高于云南白药组、iRoot BP Plus组(P<0.001);④与炎症对照组比较,云南白药组、云南白药+iRoot BP Plus组Akt、mTOR的磷酸化增加(P<0.001);⑤结果表明,云南白药、iRoot BP Plus均能促进牙髓干细胞矿化,两者联合应用矿化作用更显著;云南白药对牙髓干细胞的促矿化作用与Akt/mTOR通路相关。展开更多
基金Funded by the National Natural Science Foundation of China(Nos.50830101,51172073)the National Program on Key Basic Research Project(973 Program)(Nos.2011CB606204,2012CB619100)
文摘In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yuunan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5 bioactive glass powder （16% weight） with Vaseline and different weight percentages of Yurman baiyao. Full-thickness defect wounds were created on the back of 130 SD rats and were randomly divided into 8 groups. The wound healing rates were calculated at 4, 7, 10, 14 and 21 days after surgery. The samples were harvested for further observations. Considering the wound closure rate, group 6 （with 5% Yuunan baiyao） has better wound healing performance than other diabetic groups. The lower inflammatory response was observed by gross observation and confirmed by the results of H＆E staining and TEM observation. Besides, the proliferation of fibroblasts, the formation of granulation tissue, as well as the vascularization, were improved in group 6 compared to other diabetic groups. All results suggest that bioactive glass and Yunnan baiyao ointments can accelerate the recovery of diabetes-impaired skin wounds, and comparing to other diabetic groups, group 6 （with 5% Yunnan baiyao） has better healing effect.
文摘Objective: To observe the clinical efficacy of Yunnan Baiyao (云南白药, YNBY) combined with antibiotics in treating Helicobacter pylori (HP) related duodenal ulcer (DU). Methods: Eighty two HP positive DU patients were randomly divided into two groups: Treated group (44 cases) used YNBY plus amoxicillin and metronidazole; control group (38 cases) used de nol plus amoxicillin and metronidazole). Results: After a 2 week treatment course, the effect of epigastric pain relief was 68.3% in treated group, and 44.4% in control group, the difference between them was significant ( P <0.05). Marked effect in acid regurgitation and belching relief was observed in both groups. The rate of DU healing in treated and control group was respectively 88.6% and 89.5%; the clearance of HP 84.1% and 89.5%; and the side effects 4.5% and 13.5%, with insignificant difference between these two groups. Conclusion: YNBY combined with antibiotics in treating DU, and clearance of HP is an economic, safe and effective therapy.
文摘背景:活髓切断术现作为永久保存活髓的治疗方法,术中选择合适的盖髓材料可在较大程度上增强其临床疗效并扩大其应用范围。目的:探讨云南白药、iRoot BP Plus及其联合应用对炎症状态下牙髓干细胞矿化的影响。方法:采用组织块法培养人原代牙髓干细胞,MTT法测定云南白药、iRoot BP Plus对牙髓干细胞增殖的影响,确定最佳干预质量浓度。将第3-6代牙髓干细胞分为5组:正常对照组、炎症对照组、云南白药组、iRoot BP Plus组、云南白药+iRoot BP Plus组,后4组使用1μg/mL脂多糖诱导2 h进行细胞造模,然后分别采用75μg/mL云南白药、100μg/mL iRoot BP Plus及两者联用进行刺激,采用碱性磷酸酶染色法、茜素红S染色法、Real time-PCR法、Western blot法检测药物对细胞矿化的影响。结果与结论:①与炎症对照组比较,云南白药、iRoot BP Plus及其联合应用处理后抑制了肿瘤坏死因子αmRNA的表达(P<0.001),云南白药+iRoot BP Plus组肿瘤坏死因子αmRNA的表达明显低于云南白药组、iRoot BP Plus组(P<0.001);②与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后矿化结节数量、碱性磷酸酶活性显著增加,云南白药+iRoot BP Plus组矿化结节量、碱性磷酸酶活性高于云南白药组、iRoot BP Plus组(P<0.001);③与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后DSPP、IBSP、MEPE mRNA的表达增加(P<0.001),云南白药+iRoot BP Plus组DSPP、IBSP、MEPE mRNA的表达高于云南白药组、iRoot BP Plus组(P<0.001);④与炎症对照组比较,云南白药组、云南白药+iRoot BP Plus组Akt、mTOR的磷酸化增加(P<0.001);⑤结果表明,云南白药、iRoot BP Plus均能促进牙髓干细胞矿化,两者联合应用矿化作用更显著;云南白药对牙髓干细胞的促矿化作用与Akt/mTOR通路相关。
