Summary: Mice with immune induced aplastic anemia (AA) were given 5 mg ligustrazine intraperitoneally twice a day. On the 14th day, the expression of CD 49d , CD 49e , cyclinD 2 in bone marrow mononuclear ...Summary: Mice with immune induced aplastic anemia (AA) were given 5 mg ligustrazine intraperitoneally twice a day. On the 14th day, the expression of CD 49d , CD 49e , cyclinD 2 in bone marrow mononuclear cells (MNC) was examined by flow cytometry, and VCAM 1 on stromal cells was immunohistochemically measured by Strept Avidin Biotin Complex (SABC). The expression of CD 49d , CD 49e , VCAM 1 and cyclinD 2 in ligustrazine treated group was significantly higher than that in AA group ( P <0 01), but the ratio of G 0+G 1 phase cells was significantly lower than that in AA group ( P <0.01).The results showed that ligustrazine could improve the expression of adherent molecule and cyclin D 2 in the bone marrow of mice with immune induced aplastic anemia, thereby promoting the growth of hematopoietic cells.展开更多
After irradiation by 8.0 Gy γ ray, each mouse was stomach fed by 4 mg ligustrazine injection twice a day. On the 7th day after irradiation, CD 49 d expression in ligustrazine treated group was significantly h...After irradiation by 8.0 Gy γ ray, each mouse was stomach fed by 4 mg ligustrazine injection twice a day. On the 7th day after irradiation, CD 49 d expression in ligustrazine treated group was significantly higher than that in control group ( P <0.01), and showed no difference from that in normal group ( P > 0.05). On the 14th day after irradiation, CD 49 d expression was increased in control group, but decreased significantly in ligustrazine treated group ( P <0.01). The expression of Cyclin D 2 in spleen mononuclear cells (MNC) in ligustrazine treated group was significantly higher than that in control group, but the ratio of G 0+G 1 phase cells was significantly lower in ligustrazine treated group ( P <0.01). This finding indicated that ligustrazine could increase the expression of adherent molecule on bone marrow hematopoietic cells and Cyclin D 2 in spleen MNC, thereby promoting the growth of hematopoietic cells.展开更多
文摘Summary: Mice with immune induced aplastic anemia (AA) were given 5 mg ligustrazine intraperitoneally twice a day. On the 14th day, the expression of CD 49d , CD 49e , cyclinD 2 in bone marrow mononuclear cells (MNC) was examined by flow cytometry, and VCAM 1 on stromal cells was immunohistochemically measured by Strept Avidin Biotin Complex (SABC). The expression of CD 49d , CD 49e , VCAM 1 and cyclinD 2 in ligustrazine treated group was significantly higher than that in AA group ( P <0 01), but the ratio of G 0+G 1 phase cells was significantly lower than that in AA group ( P <0.01).The results showed that ligustrazine could improve the expression of adherent molecule and cyclin D 2 in the bone marrow of mice with immune induced aplastic anemia, thereby promoting the growth of hematopoietic cells.
文摘After irradiation by 8.0 Gy γ ray, each mouse was stomach fed by 4 mg ligustrazine injection twice a day. On the 7th day after irradiation, CD 49 d expression in ligustrazine treated group was significantly higher than that in control group ( P <0.01), and showed no difference from that in normal group ( P > 0.05). On the 14th day after irradiation, CD 49 d expression was increased in control group, but decreased significantly in ligustrazine treated group ( P <0.01). The expression of Cyclin D 2 in spleen mononuclear cells (MNC) in ligustrazine treated group was significantly higher than that in control group, but the ratio of G 0+G 1 phase cells was significantly lower in ligustrazine treated group ( P <0.01). This finding indicated that ligustrazine could increase the expression of adherent molecule on bone marrow hematopoietic cells and Cyclin D 2 in spleen MNC, thereby promoting the growth of hematopoietic cells.
