Anti-virus prophylactic therapy may be not nec- essary for the prevention of hepatitis B virus （HBV） recur- rence after HBV-related liver transplantation （LT）. However, studies on completely stopping the hepatitis...Anti-virus prophylactic therapy may be not nec- essary for the prevention of hepatitis B virus （HBV） recur- rence after HBV-related liver transplantation （LT）. However, studies on completely stopping the hepatitis B immune globu- lin （HBIG） and nucleos（t）ide analogs （NUC） after LT are few. The aim of the current study was to evaluate the safety of anti- virus prophylaxis withdrawal in liver recipients whose serum hepatitis B e antigen （HBeAg） and HBV DNA are negative. We analyzed 190 patients undergone LT for HBV-related liver dis- ease from 2006 to 2012 and found that 10 patients completely stopped the HBIG and NUC due to poor compliance. These patients were liver biopsied and checked monthly with serum HBV markers, HBV DNA and liver function. Among the 10 patients, 9 did not show the signs of HBV recurrence after a mean follow-up of 51.6 months （range 20-73） after with- drawal of the HBIG and NUC. The average time from LT to the withdrawal of the anti-virus drug was 23.8 （13-42） months; one patient showed hepatitis B surface antigen-positive and detectable HBV DNA after stopping anti-virus drugs and this patient was successfully treated with entecavir. Our data sug- gested that complete withdrawal of anti-virus prophylaxis was safe and feasible for patients whose serum HBeAg and HBV DNA were negative at the time of LT.展开更多
The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to un...The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to uniform particulate microcapsule with sustained-release properties.The polypropylene punched felt was finished with the finishing agent of microcapsule,nano ZnO and TiO 2 and polymer adhesive,and the composite air filter with anti-virus and anti-bacteria properties were formed,staphylococcus aureus,colibacillus and candida albicans were applied to antibacterial experiments.The results indicate that the anti-bacteria rate are all 100%,and the virus inactivation rate also reaches 100% to pandemic influenza A virus.展开更多
Plant Dicer-like(DCL)and Argonaute(AGO)are the key enzymes involved in anti-virus post-transcriptional gene silencing(AV-PTGS).Here we show that AV-PTGS exhibited nucleotide preference by calculating a relative AV-PTG...Plant Dicer-like(DCL)and Argonaute(AGO)are the key enzymes involved in anti-virus post-transcriptional gene silencing(AV-PTGS).Here we show that AV-PTGS exhibited nucleotide preference by calculating a relative AV-PTGS efficiency on processing viral RNA substrates.In comparison with genome sequences of dicot-infecting Turnip mosaic virus(TuMV)and monocot-infecting Cocksfoot streak virus(CSV),viral-derived small interfering RNAs(vsiRNAs)displayed positive correlations between AV-PTGS efficiency and G+C content(GC%).Further investigations on nucleotide contents revealed that the vsiRNA populations had G-biases.This finding was further supported by our analyses of previously reported vsiRNA populations in diverse plant-virus associations,and AGO associated Arabidopsis endogenous siRNA populations,indicating that plant AGOs operated with G-preference.We further propose a hypothesis that AV-PTGS imposes selection pressure(s)on the evolution of plant viruses.This hypothesis was supported when potyvirus genomes were analysed for evidence of GC elimination,suggesting that plant virus evolution to have low GC%genomes would have a unique function,which is to reduce the host AV-PTGS attack during infections.展开更多
基金supported by grants from the National Natural Science Foundation of China(81373160 and 81400673)Zhejiang Provincial Natural Science Foundation of China(LQ15H030003 and LY14H160022)
文摘Anti-virus prophylactic therapy may be not nec- essary for the prevention of hepatitis B virus （HBV） recur- rence after HBV-related liver transplantation （LT）. However, studies on completely stopping the hepatitis B immune globu- lin （HBIG） and nucleos（t）ide analogs （NUC） after LT are few. The aim of the current study was to evaluate the safety of anti- virus prophylaxis withdrawal in liver recipients whose serum hepatitis B e antigen （HBeAg） and HBV DNA are negative. We analyzed 190 patients undergone LT for HBV-related liver dis- ease from 2006 to 2012 and found that 10 patients completely stopped the HBIG and NUC due to poor compliance. These patients were liver biopsied and checked monthly with serum HBV markers, HBV DNA and liver function. Among the 10 patients, 9 did not show the signs of HBV recurrence after a mean follow-up of 51.6 months （range 20-73） after with- drawal of the HBIG and NUC. The average time from LT to the withdrawal of the anti-virus drug was 23.8 （13-42） months; one patient showed hepatitis B surface antigen-positive and detectable HBV DNA after stopping anti-virus drugs and this patient was successfully treated with entecavir. Our data sug- gested that complete withdrawal of anti-virus prophylaxis was safe and feasible for patients whose serum HBeAg and HBV DNA were negative at the time of LT.
基金supported by the National Science and Technology Small and Medium Sized Enterprises Technological Innovation Fund (Grant No.10C26213201127)Key Laboratory Foundation of Shannxi Province Education Department (Grant No.11JS044)the Shannxi Province Science and Technique Innovation Fund (Grant No. 2010K10-09)
文摘The anti-virus and anti-bacteria active components were extracted from some Chinese medicine,such as the honeysuckle,forsythia and the licorice.Using a w/o/w emulsion method,the active components were fabricated to uniform particulate microcapsule with sustained-release properties.The polypropylene punched felt was finished with the finishing agent of microcapsule,nano ZnO and TiO 2 and polymer adhesive,and the composite air filter with anti-virus and anti-bacteria properties were formed,staphylococcus aureus,colibacillus and candida albicans were applied to antibacterial experiments.The results indicate that the anti-bacteria rate are all 100%,and the virus inactivation rate also reaches 100% to pandemic influenza A virus.
基金supported by the Vietnamese Studentship to TH(Ministry of Education and Training,Decision No 322/QD-TTg)NERC(UK)grants to TD(NER/A/S/2003/00547)+1 种基金HW(NER/A/S/2003/00548,NE/E008933/1)CEH Biodiversity research fund to HW(C02875).
文摘Plant Dicer-like(DCL)and Argonaute(AGO)are the key enzymes involved in anti-virus post-transcriptional gene silencing(AV-PTGS).Here we show that AV-PTGS exhibited nucleotide preference by calculating a relative AV-PTGS efficiency on processing viral RNA substrates.In comparison with genome sequences of dicot-infecting Turnip mosaic virus(TuMV)and monocot-infecting Cocksfoot streak virus(CSV),viral-derived small interfering RNAs(vsiRNAs)displayed positive correlations between AV-PTGS efficiency and G+C content(GC%).Further investigations on nucleotide contents revealed that the vsiRNA populations had G-biases.This finding was further supported by our analyses of previously reported vsiRNA populations in diverse plant-virus associations,and AGO associated Arabidopsis endogenous siRNA populations,indicating that plant AGOs operated with G-preference.We further propose a hypothesis that AV-PTGS imposes selection pressure(s)on the evolution of plant viruses.This hypothesis was supported when potyvirus genomes were analysed for evidence of GC elimination,suggesting that plant virus evolution to have low GC%genomes would have a unique function,which is to reduce the host AV-PTGS attack during infections.
