目的:探究他克莫司联合窄谱UVB治疗特应性皮炎的疗效。方法:选取2021年2月-2022年12月笔者医院就诊120例的特应性皮炎患者,并按照简单随机化随机分为A组(采用他克莫司治疗,40例)、B组(采用润肤霜联合窄谱UVB治疗,40例)和C组(采用他克莫...目的:探究他克莫司联合窄谱UVB治疗特应性皮炎的疗效。方法:选取2021年2月-2022年12月笔者医院就诊120例的特应性皮炎患者,并按照简单随机化随机分为A组(采用他克莫司治疗,40例)、B组(采用润肤霜联合窄谱UVB治疗,40例)和C组(采用他克莫司联合窄谱UVB治疗,40例),持续治疗2个月。于治疗前、治疗1个月和治疗2个月后,比较三组特应性皮炎患者的临床体征[特应性皮炎评分量表(Scoring atopic dermatitis index,SCORAD)、中文版特应性皮炎控制工具量表(Chinese version of atopic dermatitis control tool,ADCT)]、瘙痒情况[峰值瘙痒数字评定量表(Peak pruritis-numerical rating scale,PP-NRS)]、细胞因子[血清载脂蛋白A1(Apolipoprotein A1,ApoA1)、载脂蛋白B(Apolipoprotein B,ApoB)、白细胞介素4(IL-4)、白细胞介素10(IL-10)]水平、美学效果[皮炎整体面积、客观体征评分(红斑、表皮剥脱、苔藓样变)]和生活质量[皮肤病生活质量量表(DLQI)]。记录治疗期间三组患者的不良反应,比较其治疗后3个月随访期间复发情况。结果:治疗1个月和治疗2个月后,三组的SCORAD、ADCT、PP-NRS、ApoB、IL-4、IL-10、皮炎整体面积、进行客观体征评分和DLQI评分均较治疗前显著下降,且C组显著低于A组和B组(均P<0.05);三组的ApoA1均较治疗前显著提高,且C组显著高于A组和B组(均P<0.05)。治疗期间,三组患者的不良反应发生率无显著差异(P>0.05);治疗后3个月随访期间,C组的复发情况显著低于A组和B组(P<0.05)。结论:他克莫司联合窄谱UVB治疗在特应性皮炎患者中具有显著的治疗效果,能够有效地缓解特应性皮炎症状和瘙痒情况,改善患者生活质量,提升美学效果。展开更多
Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have ...Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have been confirmed by logging,coring,and production tests in the foraminifera-rich silty sediments with complex bottom-simulating reflectors(BSRs).The broad-band processing is conducted on conventional three-dimensional(3D)seismic data to improve the image and detection accuracy of gas hydratebearing layers and delineate the saturation and thickness of gas hydrate-and free gas-bearing sediments.Several geophysical attributes extracted along the base of the gas hydrate stability zone are used to demonstrate the variable distribution and the controlling factors for the differential enrichment of gas hydrate.The inverted gas hydrate saturation at the production zone is over 40% with a thickness of 90 m,showing the interbedded distribution with different boundaries between gas hydrate-and free gas-bearing layers.However,the gas hydrate saturation value at the adjacent canyon is 70%,with 30-m-thick patches and linear features.The lithological and fault controls on gas hydrate and free gas distributions are demonstrated by tracing each gas hydrate-bearing layer.Moreover,the BSR depths based on broad-band reprocessed 3D seismic data not only exhibit variations due to small-scale topographic changes caused by seafloor sedimentation and erosion but also show the upward shift of BSR and the blocky distribution of the coexistence of gas hydrate and free gas in the Pearl River Mouth Basin.展开更多
目的:观察司库奇尤单抗联合窄谱中波紫外线(UVB)照射治疗中重度斑块型银屑病疗效及对患者银屑病面积和严重程度指数(Psoriasis area and severity index,PASI)评分及血清免疫球蛋白、炎症因子的影响。方法:选取2021年1月-2023年7月笔者...目的:观察司库奇尤单抗联合窄谱中波紫外线(UVB)照射治疗中重度斑块型银屑病疗效及对患者银屑病面积和严重程度指数(Psoriasis area and severity index,PASI)评分及血清免疫球蛋白、炎症因子的影响。方法:选取2021年1月-2023年7月笔者医院皮肤科就诊的80例中重度斑块型银屑病患者,按随机数字表法分为观察组和对照组各40例。对照组予以窄谱UVB照射治疗,观察组予以司库奇尤单抗联合窄谱UVB照射治疗。两组患者均治疗观察12周,评价临床疗效、治疗前后PASI及皮肤病生活质量指数(Dermatology life quality index,DLQI)评分;检测患者血清免疫球蛋白(IgE、IgA、IgG)及白介素-17(IL-17)、白介素-23(IL-23)、肿瘤坏死因子-α(TNF-α)水平,记录不良反应情况。结果:治疗后,观察组总有效率95.00%,高于对照组的80.00%(P<0.05)。治疗后,两组患者PASI、DLQI评分均较治疗前下降,且观察组PASI、DLQI评分均较对照组低(P<0.05)。治疗后,两组患者血清IgE、IgA、IgG及IL-17、IL-23、TNF-α水平均较治疗前下降,且观察组血清IgE、IgA、IgG及IL-17、IL-23、TNF-α水平均较对照组低(P<0.05)。观察组不良反应总发生率15.00%,与对照组的7.50%比较差异无统计学意义(P>0.05)。结论:司库奇尤单抗联合窄谱UVB照射治疗中重度斑块型银屑病,相较单独窄谱UVB治疗效果更佳,有助于减轻症状、调节免疫功能、抑制炎症水平,对提高患者生活质量有积极意义,且未明显增加不良反应。展开更多
Solar ultraviolet B(UVB)radiation is a major skin cancer-causing agent.Initiation,promotion,and progression are the diverse phases of UVB-induced carcinogenesis.Exposure to UVB causes abnormalities in a series of bioc...Solar ultraviolet B(UVB)radiation is a major skin cancer-causing agent.Initiation,promotion,and progression are the diverse phases of UVB-induced carcinogenesis.Exposure to UVB causes abnormalities in a series of biochemical and molecular pathways:thymine dimer formation,DNA damage,oxidative stress,inflammatory responses,and altered cell signaling,eventually resulting in tumor formation.The increased skin cancer rates urge researchers to develop more efficient drugs,but synthetic chemotherapeutic drugs have more contrary effects and drug resistance issues,which have been reported recently.The current review focuses on the relationship between microbes and cancer.Human skin acts as a barrier against the external environment and serves as a protective shield for its inhabitant microbiota,collectively called skin microbes.The gut microbiome plays a vital role in cancer therapy.Production of short-chain fatty acids(SCFAs)such as butyrate,acetate,and propionate by intestinal microbes has anti-cancer properties against various cancer cell lines.Yet,the knowledge of SCFAs produced by skin microbes remains yet to be elucidated exhaustively.In this review,we strive to summarize the findings of studies performed to date regarding the anti-cancer properties of SCFA against various cancer cell lines and provide insight into future directions in the skin microbiome field.展开更多
[Objectives]Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus(TFFAI)and its resistance activity to ultraviolet radiation were investigated in present research.[Methods]The optimal extra...[Objectives]Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus(TFFAI)and its resistance activity to ultraviolet radiation were investigated in present research.[Methods]The optimal extraction conditions of TFFAI were determined by single factor and orthogonal experiments,and the survival rate of TFFAI on HaCaT cells irradiated with UVB rays was investigated.It s antioxidant capacity was determined by ABTS.[Results]The results showed that the highest yield of TFFAI was obtained with 70%ethanol at a solid-to-liquid ratio of 1:50(w/v)and 40℃for 1.5 h by single-factor and orthogonal experiments.Total flavonoids(0.25-1.00 mg/ml)could significantly improve the survival rate of HaCaT cell line.Meanwhile,the maximum absorption peak of TFFAI was found at 283 nm,and in-vitro antioxidant experiment identified that TFFAI had a good clearance rate to ABTS.It suggestes that TFFAI could protect the cells from UVB damage by absorption of UVB rays and anti-oxidation.[Conclusions]TFFAI played a protective role on UVB irradiated cells through UVB ultraviolet absorption and antioxidant pathways.展开更多
文摘目的:探究他克莫司联合窄谱UVB治疗特应性皮炎的疗效。方法:选取2021年2月-2022年12月笔者医院就诊120例的特应性皮炎患者,并按照简单随机化随机分为A组(采用他克莫司治疗,40例)、B组(采用润肤霜联合窄谱UVB治疗,40例)和C组(采用他克莫司联合窄谱UVB治疗,40例),持续治疗2个月。于治疗前、治疗1个月和治疗2个月后,比较三组特应性皮炎患者的临床体征[特应性皮炎评分量表(Scoring atopic dermatitis index,SCORAD)、中文版特应性皮炎控制工具量表(Chinese version of atopic dermatitis control tool,ADCT)]、瘙痒情况[峰值瘙痒数字评定量表(Peak pruritis-numerical rating scale,PP-NRS)]、细胞因子[血清载脂蛋白A1(Apolipoprotein A1,ApoA1)、载脂蛋白B(Apolipoprotein B,ApoB)、白细胞介素4(IL-4)、白细胞介素10(IL-10)]水平、美学效果[皮炎整体面积、客观体征评分(红斑、表皮剥脱、苔藓样变)]和生活质量[皮肤病生活质量量表(DLQI)]。记录治疗期间三组患者的不良反应,比较其治疗后3个月随访期间复发情况。结果:治疗1个月和治疗2个月后,三组的SCORAD、ADCT、PP-NRS、ApoB、IL-4、IL-10、皮炎整体面积、进行客观体征评分和DLQI评分均较治疗前显著下降,且C组显著低于A组和B组(均P<0.05);三组的ApoA1均较治疗前显著提高,且C组显著高于A组和B组(均P<0.05)。治疗期间,三组患者的不良反应发生率无显著差异(P>0.05);治疗后3个月随访期间,C组的复发情况显著低于A组和B组(P<0.05)。结论:他克莫司联合窄谱UVB治疗在特应性皮炎患者中具有显著的治疗效果,能够有效地缓解特应性皮炎症状和瘙痒情况,改善患者生活质量,提升美学效果。
基金supported by the State Key Laboratory of Natural Gas Hydrate(No.2022-KFJJ-SHW)the National Natural Science Foundation of China(No.42376058)+2 种基金the International Science&Technology Cooperation Program of China(No.2023YFE0119900)the Hainan Province Key Research and Development Project(No.ZDYF2024GXJS002)the Research Start-Up Funds of Zhufeng Scholars Program.
文摘Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have been confirmed by logging,coring,and production tests in the foraminifera-rich silty sediments with complex bottom-simulating reflectors(BSRs).The broad-band processing is conducted on conventional three-dimensional(3D)seismic data to improve the image and detection accuracy of gas hydratebearing layers and delineate the saturation and thickness of gas hydrate-and free gas-bearing sediments.Several geophysical attributes extracted along the base of the gas hydrate stability zone are used to demonstrate the variable distribution and the controlling factors for the differential enrichment of gas hydrate.The inverted gas hydrate saturation at the production zone is over 40% with a thickness of 90 m,showing the interbedded distribution with different boundaries between gas hydrate-and free gas-bearing layers.However,the gas hydrate saturation value at the adjacent canyon is 70%,with 30-m-thick patches and linear features.The lithological and fault controls on gas hydrate and free gas distributions are demonstrated by tracing each gas hydrate-bearing layer.Moreover,the BSR depths based on broad-band reprocessed 3D seismic data not only exhibit variations due to small-scale topographic changes caused by seafloor sedimentation and erosion but also show the upward shift of BSR and the blocky distribution of the coexistence of gas hydrate and free gas in the Pearl River Mouth Basin.
文摘目的:观察司库奇尤单抗联合窄谱中波紫外线(UVB)照射治疗中重度斑块型银屑病疗效及对患者银屑病面积和严重程度指数(Psoriasis area and severity index,PASI)评分及血清免疫球蛋白、炎症因子的影响。方法:选取2021年1月-2023年7月笔者医院皮肤科就诊的80例中重度斑块型银屑病患者,按随机数字表法分为观察组和对照组各40例。对照组予以窄谱UVB照射治疗,观察组予以司库奇尤单抗联合窄谱UVB照射治疗。两组患者均治疗观察12周,评价临床疗效、治疗前后PASI及皮肤病生活质量指数(Dermatology life quality index,DLQI)评分;检测患者血清免疫球蛋白(IgE、IgA、IgG)及白介素-17(IL-17)、白介素-23(IL-23)、肿瘤坏死因子-α(TNF-α)水平,记录不良反应情况。结果:治疗后,观察组总有效率95.00%,高于对照组的80.00%(P<0.05)。治疗后,两组患者PASI、DLQI评分均较治疗前下降,且观察组PASI、DLQI评分均较对照组低(P<0.05)。治疗后,两组患者血清IgE、IgA、IgG及IL-17、IL-23、TNF-α水平均较治疗前下降,且观察组血清IgE、IgA、IgG及IL-17、IL-23、TNF-α水平均较对照组低(P<0.05)。观察组不良反应总发生率15.00%,与对照组的7.50%比较差异无统计学意义(P>0.05)。结论:司库奇尤单抗联合窄谱UVB照射治疗中重度斑块型银屑病,相较单独窄谱UVB治疗效果更佳,有助于减轻症状、调节免疫功能、抑制炎症水平,对提高患者生活质量有积极意义,且未明显增加不良反应。
文摘Solar ultraviolet B(UVB)radiation is a major skin cancer-causing agent.Initiation,promotion,and progression are the diverse phases of UVB-induced carcinogenesis.Exposure to UVB causes abnormalities in a series of biochemical and molecular pathways:thymine dimer formation,DNA damage,oxidative stress,inflammatory responses,and altered cell signaling,eventually resulting in tumor formation.The increased skin cancer rates urge researchers to develop more efficient drugs,but synthetic chemotherapeutic drugs have more contrary effects and drug resistance issues,which have been reported recently.The current review focuses on the relationship between microbes and cancer.Human skin acts as a barrier against the external environment and serves as a protective shield for its inhabitant microbiota,collectively called skin microbes.The gut microbiome plays a vital role in cancer therapy.Production of short-chain fatty acids(SCFAs)such as butyrate,acetate,and propionate by intestinal microbes has anti-cancer properties against various cancer cell lines.Yet,the knowledge of SCFAs produced by skin microbes remains yet to be elucidated exhaustively.In this review,we strive to summarize the findings of studies performed to date regarding the anti-cancer properties of SCFA against various cancer cell lines and provide insight into future directions in the skin microbiome field.
基金Supported by National Key Program of Innovation and Entrepreneurship Training for College Students (202211834021)Project Funds of Zhengzhou Science and Technology Bureau (ZZSZX202109).
文摘[Objectives]Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus(TFFAI)and its resistance activity to ultraviolet radiation were investigated in present research.[Methods]The optimal extraction conditions of TFFAI were determined by single factor and orthogonal experiments,and the survival rate of TFFAI on HaCaT cells irradiated with UVB rays was investigated.It s antioxidant capacity was determined by ABTS.[Results]The results showed that the highest yield of TFFAI was obtained with 70%ethanol at a solid-to-liquid ratio of 1:50(w/v)and 40℃for 1.5 h by single-factor and orthogonal experiments.Total flavonoids(0.25-1.00 mg/ml)could significantly improve the survival rate of HaCaT cell line.Meanwhile,the maximum absorption peak of TFFAI was found at 283 nm,and in-vitro antioxidant experiment identified that TFFAI had a good clearance rate to ABTS.It suggestes that TFFAI could protect the cells from UVB damage by absorption of UVB rays and anti-oxidation.[Conclusions]TFFAI played a protective role on UVB irradiated cells through UVB ultraviolet absorption and antioxidant pathways.
