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Differential Diagnostic Value of Texture Feature Analysis of Magnetic Resonance T2 Weighted Imaging between Glioblastoma and Primary Central Neural System Lymphoma 被引量:5
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作者 王波涛 刘明霞 陈志晔 《Chinese Medical Sciences Journal》 CAS CSCD 2019年第1期10-17,共8页
Objective To investigate the difference in tumor conventional imaging findings and texture features on T2 weighted images between glioblastoma and primary central neural system(CNS) lymphoma. Methods The pre-operative... Objective To investigate the difference in tumor conventional imaging findings and texture features on T2 weighted images between glioblastoma and primary central neural system(CNS) lymphoma. Methods The pre-operative MRI data of 81 patients with glioblastoma and 28 patients with primary CNS lymphoma admitted to the Chinese PLA General Hospital and Hainan Hospital of Chinese PLA General Hospital were retrospectively collected. All patients underwent plain MR imaging and enhanced T1 weighted imaging to visualize imaging features of lesions. Texture analysis of T2 weighted imaging(T2 WI) was performed by use of GLCM texture plugin of ImageJ software, and the texture parameters including Angular Second Moment(ASM), Contrast, Correlation, Inverse Difference Moment(IDM), and Entropy were measured. Independent sample t-test and Mann-Whitney U test were performed for the between-group comparisons, regression model was established by Binary Logistic regression analysis, and receiver operating characteristic(ROC) curve was plotted to compare the diagnostic efficacy. Results The conventional imaging features including cystic and necrosis changes(P = 0.000), ‘Rosette' changes(P = 0.000) and ‘incision sign'(P = 0.000), except ‘flame-like edema'(P = 0.635), presented significantly statistical difference between glioblastoma and primary CNS lymphoma. The texture features, ASM, Contrast, Correlation, IDM and Entropy, showed significant differences between glioblastoma and primary CNS lympoma(P = 0.006,0.000, 0.002, 0.000, and 0.015 respectively). The area under the ROC curve was 0.671, 0.752, 0.695, 0.720 and 0.646 respectively, and the area under the ROC curve was 0.917 for the combined texture variables(Contrast, cystic and necrosis, ‘Rosette' changes, and ‘incision sign') in the model of Logistic regression. Binary Logistic regression analysis demonstrated that cystic and necrosis changes, ‘Rosette' changes and ‘incision sign' and texture Contrast could be considered as the specific texture variables for the differential diagnosis of glioblastoma and primary CNS lymphoma. Conclusion The texture features of T2 WI and conventional imaging findings may be used to distinguish glioblastoma from primary CNS lymphoma. 展开更多
关键词 GLIOBLASTOMA primary central neural system LYMPHOMA texture analysis T2 WEIGHTED imaging differential diagnosis
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Expression of Nogo-A mRNA after injury of the rat central nervous system 被引量:8
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作者 Xigao Guo Yang Guo Tao Huang 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第12期1368-1371,共4页
BACKGROUND: Nogo protein has been identified as an inhibitor of axonal growth, which was highly expressed in central nervous system; however, there are only a few studies on changes of Nogo-A expression following cen... BACKGROUND: Nogo protein has been identified as an inhibitor of axonal growth, which was highly expressed in central nervous system; however, there are only a few studies on changes of Nogo-A expression following central nervous system injury. OBJECTIVE: To investigate the dynamic expression of Nogo-A mRNA after rat central nervous system injury. DESIGN: Randomized controlled animal study. MATERIALS: Thirty-five rats were randomly divided into two groups, normal animal group (n = 5) and model group (n = 30). The model group was then divided into six subgroups at six time points: 12, 24 hours and 3, 9, 15, and 21 days post-injury, with five rats in each subgroup. METHODS: The left parietal lobe of rats was contused by free-fall strike, and total RNA was extracted from the entire brain tissue. Semi-quantitative RT-PCR was used to detect Nogo-A mRNA expression, and the ratio between expression of the target gene and glyceraldehyde phosphate dehydrogenase was used to determine the relative expression level. MAIN OUTCOME MEASURES: To determine whether Nogo-A mRNA expression was higher than usual following brain injury. RESULTS: The level of Nogo-A mRNA started to increase 12 hours after injury (P 〈 0.05) and decreased slightly by 24 hours post-injury. Expression increased again on day 3 and reached a peak on day 9. Nogo-A mRNA expression started to decrease on day 15, and then decreased to normal levels at days 21 (P 〉 0.05). CONCLUSION: After injury of the central nervous system, Nogo-A may play a pivotal role in obstructing regeneration of the nerve. 展开更多
关键词 central nervous system neural regeneration NOGO-A
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Role of nuclear factor kappa B in central nervous system regeneration 被引量:10
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作者 Christian Engelmann Falk Weih Ronny Haenold 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第7期707-711,共5页
Activation of nuclear factor kappa B (NF-κB) is a hallmark of various central nervous system (CNS) pathologies. Neuron-specific inhibition of its transcriptional activator subunit RelA, also referred to as p65, p... Activation of nuclear factor kappa B (NF-κB) is a hallmark of various central nervous system (CNS) pathologies. Neuron-specific inhibition of its transcriptional activator subunit RelA, also referred to as p65, promotes neuronal survival under a range of conditions, i.e., for ischemic or excitotoxic insults. In macro- and microglial cells, post-lesional activation of NF-κB triggers a growth-permissive program which contributes to neural tissue inflammation, scar formation, and the expression of axonal growth inhibitors. Intriguingly, inhibition of such inducible NF-~B in the neuro-glial compartment, i.e., by genetic ablation of RelA or overexpression of a trans- dominant negative mutant of its upstream regulator IκBa, significantly enhances functional recovery and promotes axonal regeneration in the mature CNS. By contrast, depletion of the NF-κB subunit p50, which lacks transcriptional activator function and acts as a transcriptional repressor on its own, causes precocious neuronal loss and exacerbates axonal degeneration in the lesioned brain. Collectively, the data imply that NF-κB orchestrates a multicellular pro- gram in which κB-dependent gene expression establishes a growth-repulsive terrain within the post-lesioned brain that limits structural regeneration of neuronal circuits. Considering these subunit-specific functions, interference with the NF-κB pathway might hold clinical potentials to improve functional restoration following traumatic CNS injury. 展开更多
关键词 nuclear factor kappa B RELA P65 P50 central nervous system injury axonal regeneration neural regeneration
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MicroRNAs of microglia: wrestling with central nervous system disease 被引量:5
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作者 Xiao-Hua Wang Tian-Long Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第12期2067-2072,共6页
Microglia serve as brain-resident myeloid cells that affect cerebral development, ischemia, neurodegeneration, and neuro-viral infection. MicroRNAs play a key role in central nervous system disease through post-transc... Microglia serve as brain-resident myeloid cells that affect cerebral development, ischemia, neurodegeneration, and neuro-viral infection. MicroRNAs play a key role in central nervous system disease through post-transcriptional regulation. Indeed, evidence shows that microRNAs are one of the most important regulators mediating microglial activation, polarization, and autophagy, and subsequently affecting neuroinflammation and the outcome of central nervous system disease. In this review, we provide insight into the function of microRNAs, which may be an attractive strategy and influential treatment for microglia-related central nervous system dysfunction. Moreover, we comprehensively describe how microglia fight against central nervous system disease via multiple functional microRNAs. 展开更多
关键词 MICROGLIA NEURODEGENERATION central nervous system disease MICRORNAS activation polarization AUTOPHAGY neural regeneration
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Modelling the impact of climate change on rangeland forage production using a generalized regression neural network:a case study in Isfahan Province,Central Iran
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作者 Zahra JABERALANSAR Mostafa TARKESH +1 位作者 Mehdi BASSIRI Saeid POURMANAFI 《Journal of Arid Land》 SCIE CSCD 2017年第4期489-503,共15页
Monitoring of rangeland forage production at specified spatial and temporal scales is necessary for grazing management and also for implementation of rehabilitation projects in rangelands. This study focused on the ca... Monitoring of rangeland forage production at specified spatial and temporal scales is necessary for grazing management and also for implementation of rehabilitation projects in rangelands. This study focused on the capability of a generalized regression neural network(GRNN) model combined with GIS techniques to explore the impact of climate change on rangeland forage production. Specifically, a dataset of 115 monitored records of forage production were collected from 16 rangeland sites during the period 1998–2007 in Isfahan Province, Central Iran. Neural network models were designed using the monitored forage production values and available environmental data(including climate and topography data), and the performance of each network model was assessed using the mean estimation error(MEE), model efficiency factor(MEF), and correlation coefficient(r). The best neural network model was then selected and further applied to predict the forage production of rangelands in the future(in 2030 and 2080) under A1 B climate change scenario using Hadley Centre coupled model. The present and future forage production maps were also produced. Rangeland forage production exhibited strong correlations with environmental factors, such as slope, elevation, aspect and annual temperature. The present forage production in the study area varied from 25.6 to 574.1 kg/hm^2. Under climate change scenario, the annual temperature was predicted to increase and the annual precipitation was predicted to decrease. The prediction maps of forage production in the future indicated that the area with low level of forage production(0–100 kg/hm^2) will increase while the areas with moderate, moderately high and high levels of forage production(≥100 kg/hm^2) will decrease both in 2030 and in 2080, which may be attributable to the increasing annual temperature and decreasing annual precipitation. It was predicted that forage production of rangelands will decrease in the next couple of decades, especially in the western and southern parts of Isfahan Province. These changes are more pronounced in elevations between 2200 and 2900 m. Therefore, rangeland managers have to cope with these changes by holistic management approaches through mitigation and human adaptations. 展开更多
关键词 rangelands forage production climate change scenario generalized regression neural network central Iran
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The role of the Rho/ROCK signaling pathway in inhibiting axonal regeneration in the central nervous system 被引量:11
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作者 Jing Liu Hong-yan Gao Xiao-feng Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第11期1892-1896,共5页
The Rho/Rho-associated coiled-coil containing protein kinase(Rho/ROCK) pathway is a major signaling pathway in the central nervous system, transducing inhibitory signals to block regeneration. After central nervous ... The Rho/Rho-associated coiled-coil containing protein kinase(Rho/ROCK) pathway is a major signaling pathway in the central nervous system, transducing inhibitory signals to block regeneration. After central nervous system damage, the main cause of impaired regeneration is the presence of factors that strongly inhibit regeneration in the surrounding microenvironment. These factors signal through the Rho/ROCK signaling pathway to inhibit regeneration. Therefore, a thorough understanding of the Rho/ROCK signaling pathway is crucial for advancing studies on regeneration and repair of the injured central nervous system. 展开更多
关键词 nerve regeneration Rho/Rho-associated coiled-coil containing protein kinase SIGNALINGPATHWAY axonal regeneration central nervous system microenvironment REVIEWS NSFC grant neural regeneration
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Taking central nervous system regenerative therapies to the clinic: curing rodents versus nonhuman primates versus humans 被引量:7
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作者 Magdalini Tsintou Kyriakos Dalamagkas Nikos Makris 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第3期425-437,共13页
The central nervous system is known to have limited regenerative capacity.Not only does this halt the human body’s reparative processes after central nervous system lesions,but it also impedes the establishment of ef... The central nervous system is known to have limited regenerative capacity.Not only does this halt the human body’s reparative processes after central nervous system lesions,but it also impedes the establishment of effective and safe therapeutic options for such patients.Despite the high prevalence of stroke and spinal cord injury in the general population,these conditions remain incurable and place a heavy burden on patients’families and on society more broadly.Neuroregeneration and neural engineering are diverse biomedical fields that attempt reparative treatments,utilizing stem cells-based strategies,biologically active molecules,nanotechnology,exosomes and highly tunable biodegradable systems(e.g.,certain hydrogels).Although there are studies demonstrating promising preclinical results,safe clinical translation has not yet been accomplished.A key gap in clinical translation is the absence of an ideal animal or ex vivo model that can perfectly simulate the human microenvironment,and also correspond to all the complex pathophysiological and neuroanatomical factors that affect functional outcomes in humans after central nervous system injury.Such an ideal model does not currently exist,but it seems that the nonhuman primate model is uniquely qualified for this role,given its close resemblance to humans.This review considers some regenerative therapies for central nervous system repair that hold promise for future clinical translation.In addition,it attempts to uncover some of the main reasons why clinical translation might fail without the implementation of nonhuman primate models in the research pipeline. 展开更多
关键词 animal models central nervous system regeneration clinical translation exosomes hydrogels neural tissue engineering nonhuman PRIMATES spinal cord injury stem cells stroke
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Mild hypothermia as a treatment for central nervous system injuries Positive or negative effects? 被引量:25
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作者 Rami Darwazeh Yi Yan 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第28期2677-2686,共10页
Besides local neuronal damage caused by the primary insult, central nervous system injuries may secondarily cause a progressive cascade of related events including brain edema, ischemia, oxida- tive stress, excitotoxi... Besides local neuronal damage caused by the primary insult, central nervous system injuries may secondarily cause a progressive cascade of related events including brain edema, ischemia, oxida- tive stress, excitotoxicity, and dysregulation of calcium homeostasis. Hypothermia is a beneficial strategy in a variety of acute central nervous system injuries. Mild hypothermia can treat high in- tracranial pressure following traumatic brain injuries in adults. It is a new treatment that increases survival and quality of life for patients suffering from ischemic insults such as cardiac arrest, stroke, and neurogenic fever following brain trauma. Therapeutic hypothermia decreases free radical pro- duction, inflammation, excitotoxicity and intracranial pressure, and improves cerebral metabolism after traumatic brain injury and cerebral ischemia, thus protecting against central nervous system damage. Although a series of pathological and physiological changes as well as potential side ef- fects are observed during hypothermia treatment, it remains a potential therapeutic strategy for central nervous system injuries and deserves further study. 展开更多
关键词 neural regeneration REVIEWS brain injury spinal cord injury central nervous system injury mildhypothermia therapeutic hypothermia traumatic brain injury NEUROREGENERATION
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Regeneration of the central nervous system-principles from brain regeneration in adult zebrafish 被引量:3
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作者 Alessandro Zambusi Jovica Ninkovic 《World Journal of Stem Cells》 SCIE 2020年第1期8-24,共17页
Poor recovery of neuronal functions is one of the most common healthcare challenges for patients with different types of brain injuries and/or neurodegenerative diseases.Therapeutic interventions face two major challe... Poor recovery of neuronal functions is one of the most common healthcare challenges for patients with different types of brain injuries and/or neurodegenerative diseases.Therapeutic interventions face two major challenges:(1)How to generate neurons de novo to replenish the neuronal loss caused by injuries or neurodegeneration(restorative neurogenesis)and(2)How to prevent or limit the secondary tissue damage caused by long-term accumulation of glial cells,including microglia,at injury site(glial scar).In contrast to mammals,zebrafish have extensive regenerative capacity in numerous vital organs,including the brain,thus making them a valuable model to improve the existing therapeutic approaches for human brain repair.In response to injuries to the central nervous system(CNS),zebrafish have developed specific mechanisms to promote the recovery of the lost tissue architecture and functionality of the damaged CNS.These mechanisms include the activation of a restorative neurogenic program in a specific set of glial cells(ependymoglia)and the resolution of both the glial scar and inflammation,thus enabling proper neuronal specification and survival.In this review,we discuss the cellular and molecular mechanisms underlying the regenerative ability in the adult zebrafish brain and conclude with the potential applicability of these mechanisms in repair of the mammalian CNS. 展开更多
关键词 ZEBRAFISH central nervous system Brain injury Glial scar REGENERATION Restorative neurogenesis neural stem cells Inflammation
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The Olig family affects central nervous system development and disease 被引量:5
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作者 Botao Tan Jing Yu +3 位作者 Ying Yin Gongwei Jia Wei Jiang Lehua Yu 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第3期329-336,共8页
Neural cell differentiation and maturation is a critical step during central nervous system devel-opment. The oligodendrocyte transcription family (Olig family) is known to be an important factor in regulating neura... Neural cell differentiation and maturation is a critical step during central nervous system devel-opment. The oligodendrocyte transcription family (Olig family) is known to be an important factor in regulating neural cell differentiation. Because of this, the Olig family also affects acute and chronic central nervous system diseases, including brain injury, multiple sclerosis, and even gliomas. Improved understanding about the functions of the Olig family in central nervous system development and disease will greatly aid novel breakthroughs in central nervous system diseases. This review investigates the role of the Olig family in central nervous system develop- ment and related diseases. 展开更多
关键词 nerve regeneration brain injury spinal cord injury review Olig family oligodendro-cytes ASTROCYTES central nervous system disease DEMYELINATION development DIFFERENTIATION NSFCgrant neural regeneration
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外周骨骼肌肉疾病致中枢神经重塑的研究进展
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作者 谢惠敏 杨作辉 +4 位作者 李滨 常祺 刘昕怡 张科学 张立宁 《解放军医学院学报》 CAS 2024年第7期799-804,共6页
外周骨骼肌肉疾病是常见的慢性病,主要涉及全身或局部的骨骼、肌肉或结缔组织,常引起局部疼痛、酸胀或运动障碍。感觉输入异常或运动模式改变,均会引起中枢神经的适应性改变,发生中枢神经重塑,而中枢神经重塑又可能进一步造成运动系统... 外周骨骼肌肉疾病是常见的慢性病,主要涉及全身或局部的骨骼、肌肉或结缔组织,常引起局部疼痛、酸胀或运动障碍。感觉输入异常或运动模式改变,均会引起中枢神经的适应性改变,发生中枢神经重塑,而中枢神经重塑又可能进一步造成运动系统适应性不良和感觉异常。现有研究通过多模态神经影像学、脑电图、脑磁图、功能近红外血氧分析等方法,发现外周骨骼肌肉疾病患者的疼痛/情绪调节相关脑区或通路、运动感觉皮质、平衡相关脑区、任务执行网络、默认网络等发生了重塑改变;也有学者分析了细胞层面的重塑机制,认为可能是突触数量或突触连接的改变造成了重塑。本文重点介绍外周骨骼肌肉疾病的发生发展及转归与中枢神经重塑的关系。 展开更多
关键词 骨骼肌肉疾病 神经重塑 中枢神经 神经可塑性
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基于改进蚁群算法的电力集控系统故障诊断方法
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作者 冯钢 《电子设计工程》 2024年第18期97-100,共4页
针对提高供电连续性和可靠性,保证电能质量的目的,采用基于改进蚁群算法,提出了电力集控系统故障诊断方法。通过传感器和数据传输接口形式获取电力集控系统实时运行信息,将其作为输入,建立电力集控系统故障诊断模型。运用改进蚁群算法... 针对提高供电连续性和可靠性,保证电能质量的目的,采用基于改进蚁群算法,提出了电力集控系统故障诊断方法。通过传感器和数据传输接口形式获取电力集控系统实时运行信息,将其作为输入,建立电力集控系统故障诊断模型。运用改进蚁群算法对电力集控系统故障诊断模型进行求解,通过蚂蚁迭代方式得到电力集控系统故障诊断模型的最优解,实现电力集控系统故障诊断模型求解。实验表明,该方法具备较好的电力集控系统故障特征提取能力和故障诊断能力,同时其故障诊断时的容错率数值较低,数值在0.2%上下波动,求解电力集控系统故障诊断模型的迭代能力较强。 展开更多
关键词 改进蚁群算法 电力集控系统 故障诊断 神经网络 优化求解
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涡北煤矿地面集中式降温控制系统设计与应用
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作者 汪开松 《山东煤炭科技》 2024年第2期152-155,160,165,共6页
针对涡北煤矿降温项目设计一套集“管”“控”为一体的集中控制系统,重点分析了该系统的网络架构、硬件组成及软件应用,详细描述了该系统实现的主要功能。提出基于BP神经网络PID控制算法对系统优化,以实现二次冷量的精准分配,达到节能... 针对涡北煤矿降温项目设计一套集“管”“控”为一体的集中控制系统,重点分析了该系统的网络架构、硬件组成及软件应用,详细描述了该系统实现的主要功能。提出基于BP神经网络PID控制算法对系统优化,以实现二次冷量的精准分配,达到节能减排的效果。在人机交互界面中引入3D建模技术,为矿方提供更加简洁、功能齐全、友好的人机交互界面,通过通信技术将整个降温系统的子系统全部纳入集中监控系统,提高了降温系统的生产效率,达到减员增效的效果,并预留与矿井调度通信接口,为矿井综合自动化系统提供相关基础数据。 展开更多
关键词 矿井降温 集中监控系统 BP神经网络 子系统融合
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Neural differentiation of choroid plexus epithelial cells:role of human traumatic cerebrospinal fluid 被引量:1
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作者 Elham Hashemi Yousef Sadeghi +6 位作者 Abbas Aliaghaei Afsoun Seddighi Abbas Piryaei Mehdi Eskandarian Broujeni Fatemeh Shaerzadeh Abdollah Amini Ramin Pouriran 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第1期84-89,共6页
As the key producer of cerebrospinal fluid(CSF),the choroid plexus(CP) provides a unique protective system in the central nervous system.CSF components are not invariable and they can change based on the pathologi... As the key producer of cerebrospinal fluid(CSF),the choroid plexus(CP) provides a unique protective system in the central nervous system.CSF components are not invariable and they can change based on the pathological conditions of the central nervous system.The purpose of the present study was to assess the effects of non-traumatic and traumatic CSF on the differentiation of multipotent stem-like cells of CP into the neural and/or glial cells.CP epithelial cells were isolated from adult male rats and treated with human non-traumatic and traumatic CSF.Alterations in m RNA expression of Nestin and microtubule-associated protein(MAP2),as the specific markers of neurogenesis,and astrocyte marker glial fibrillary acidic protein(GFAP) in cultured CP epithelial cells were evaluated using quantitative real-time PCR.The data revealed that treatment with CSF(non-traumatic and traumatic) led to increase in m RNA expression levels of MAP2 and GFAP.Moreover,the expression of Nestin decreased in CP epithelial cells treated with non-traumatic CSF,while treatment with traumatic CSF significantly increased its m RNA level compared to the cells cultured only in DMEM/F12 as control.It seems that CP epithelial cells contain multipotent stem-like cells which are inducible under pathological conditions including exposure to traumatic CSF because of its compositions. 展开更多
关键词 nerve regeneration choroid plexus cerebrospinal fluid stem cells Nestin microtubule-associated protein 2 glial fibrillary acidic protein neurogenesis central nervous system neural regeneration
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Neural regeneration by regionally induced stem cells within poststroke brains: Novel therapy perspectives for stroke patients 被引量:1
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作者 Takayuki Nakagomi Toshinori Takagi +2 位作者 Mikiya Beppu Shinichi Yoshimura Tomohiro Matsuyama 《World Journal of Stem Cells》 SCIE 2019年第8期452-463,共12页
Ischemic stroke is a critical disease which causes serious neurological functional loss such as paresis. Hope for novel therapies is based on the increasing evidence of the presence of stem cell populations in the cen... Ischemic stroke is a critical disease which causes serious neurological functional loss such as paresis. Hope for novel therapies is based on the increasing evidence of the presence of stem cell populations in the central nervous system (CNS) and the development of stem-cell-based therapies for stroke patients. Although mesenchymal stem cells (MSCs) represented initially a promising cell source, only a few transplanted MSCs were present near the injured areas of the CNS. Thus, regional stem cells that are present and/or induced in the CNS may be ideal when considering a treatment following ischemic stroke. In this context, we have recently showed that injury/ischemia-induced neural stem/progenitor cells (iNSPCs) and injury/ischemia-induced multipotent stem cells (iSCs) are present within post-stroke human brains and post-stroke mouse brains. This indicates that iNSPCs/iSCs could be developed for clinical applications treating patients with stroke. The present study introduces the traits of mouse and human iNSPCs, with a focus on the future perspective for CNS regenerative therapies using novel iNSPCs/iSCs. 展开更多
关键词 Ischemic STROKE STROKE patients central nervous system neural stem/progenitor CELLS MULTIPOTENT STEM CELLS Stem-cell-based therapies
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Distribution of paired immunoglobulin-like receptor B in the nervous system related to regeneration difficulties after unilateral lumbar spinal cord injury 被引量:3
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作者 Wan-shu Peng Chao Qi +4 位作者 Hong Zhang Mei-ling Gao Hong Wang Fei Ren Xia-qing Li 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第7期1139-1146,共8页
Paired immunoglobulin-like receptor B(Pir B) is a functional receptor of myelin-associated inhibitors for axonal regeneration and synaptic plasticity in the central nervous system, and thus suppresses nerve regenera... Paired immunoglobulin-like receptor B(Pir B) is a functional receptor of myelin-associated inhibitors for axonal regeneration and synaptic plasticity in the central nervous system, and thus suppresses nerve regeneration. The regulatory effect of Pir B on injured nerves has received a lot of attention. To better understand nerve regeneration inability after spinal cord injury, this study aimed to investigate the distribution of Pir B(via immunofluorescence) in the central nervous system and peripheral nervous system 10 days after injury. Immunoreactivity for Pir B increased in the dorsal root ganglia, sciatic nerves, and spinal cord segments. In the dorsal root ganglia and sciatic nerves, Pir B was mainly distributed along neuronal and axonal membranes. Pir B was found to exhibit a diffuse, intricate distribution in the dorsal and ventral regions. Immunoreactivity for Pir B was enhanced in some cortical neurons located in the bilateral precentral gyri. Overall, the findings suggest a pattern of Pir B immunoreactivity in the nervous system after unilateral spinal transection injury, and also indicate that Pir B may suppress repair after injury. 展开更多
关键词 nerve regeneration paired immunoglobulin-like receptor B myelin inhibitory factor spinal cord injury peripheral nervous system central nervous system cerebral cortex dorsal root ganglion neural regeneration
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Central projections and connections of lumbar primary afferent fibers in adult rats:effectively revealed using Texas red-dextran amine tracing 被引量:1
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作者 Shi-de Lin Tao Tang +1 位作者 Ting-bao Zhao Shao-jun Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第10期1695-1702,共8页
Signals from lumbar primary afferent fibers are important for modulating locomotion of the hind-limbs.However,silver impregnation techniques,autoradiography,wheat germ agglutinin-horseradish peroxidase and cholera tox... Signals from lumbar primary afferent fibers are important for modulating locomotion of the hind-limbs.However,silver impregnation techniques,autoradiography,wheat germ agglutinin-horseradish peroxidase and cholera toxin B subunit-horseradish peroxidase cannot image the central projections and connections of the dorsal root in detail.Thus,we injected 3-k Da Texas red-dextran amine into the proximal trunks of L4 dorsal roots in adult rats.Confocal microscopy results revealed that numerous labeled arborizations and varicosities extended to the dorsal horn from T12–S4,to Clarke's column from T10–L2,and to the ventral horn from L1–5.The labeled varicosities at the L4 cord level were very dense,particularly in laminae I–Ⅲ,and the density decreased gradually in more rostral and caudal segments.In addition,they were predominately distributed in laminae I–IV,moderately in laminae V–VⅡ and sparsely in laminae VⅢ–X.Furthermore,direct contacts of lumbar afferent fibers with propriospinal neurons were widespread in gray matter.In conclusion,the projection and connection patterns of L4 afferents were illustrated in detail by Texas red-dextran amine-dorsal root tracing. 展开更多
关键词 nerve regeneration spinal cord injury dorsal root central projection connection Texas red-dextran amine neural regeneration
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Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold 被引量:5
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作者 Fei Huang Qiang Shen Jitong Zhao 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第4期313-319,共7页
Collagen protein is an ideal scaffold material for the transplantation of neural stem cells. In this study rat neural stern cells were seeded into a three-dimensional collagen gel scaffold, with suspension cultured ne... Collagen protein is an ideal scaffold material for the transplantation of neural stem cells. In this study rat neural stern cells were seeded into a three-dimensional collagen gel scaffold, with suspension cultured neural stem cells being used as a control group. Neural stem cells, which were cultured in medium containing epidermal growth factor and basic fibroblast growth factor, actively expanded and formed neurospheres in both culture groups. In serum-free medium conditions, the processes extended from neurospheres in the collagen gel group were much longer than those in the suspension culture group. Immunofluorescence staining showed that neurespheres cultured in collagen gels were stained positive for nestin and differentiated cells were stained positive for the neuronal marker βIII-tubulin, the astrocytic marker glial fibrillary acidic protein and the oligodendrocytic marker 2',3'-cyclic nucleotide 3'-phosphodiesterase. Compared with neurospheres cultured in suspension, the differentiation potential of neural stem cells cultured in collagen gels increased, with the formation of neurons at an early stage. Our results show that the three-dimensional collagen gel culture system is superior to suspension culture in the proliferation, differentiation and process outgrowth of neural stem cells. 展开更多
关键词 neural regeneration stem cells neural stem cells collagen gel SCAFFOLD central nervous system proliferation DIFFERENTIATION NEUROSPHERE photographs-containing paper NEUROREGENERATION
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中枢神经环路调节骨代谢 被引量:1
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作者 刘运辉 王立平 杨帆 《生理科学进展》 CAS 2023年第6期468-475,共8页
骨重塑是维持骨稳态(bone homeostasis)的重要过程,人们通常认为骨重塑主要接受内分泌激素调节。近年来,越来越多的证据表明,中枢神经系统通过传出神经直接参与骨稳态调节。虽然,大脑和骨骼组织之间已被证实存在明确的神经连接,但这些... 骨重塑是维持骨稳态(bone homeostasis)的重要过程,人们通常认为骨重塑主要接受内分泌激素调节。近年来,越来越多的证据表明,中枢神经系统通过传出神经直接参与骨稳态调节。虽然,大脑和骨骼组织之间已被证实存在明确的神经连接,但这些神经联系在大脑调节骨稳态中的作用还不十分清楚。本文将关注骨与脑之间的神经联系,分别从中枢神经系统和神经环路(neural circuit)、自主神经系统以及感觉神经系统对骨稳态的调控等方面介绍大脑调控骨代谢(bone metabolism)领域的最新进展。 展开更多
关键词 神经调控 骨代谢 骨稳态 神经环路 中枢神经系统 自主神经系统 感觉神经系统
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星形胶质细胞来源细胞外囊泡促进大鼠神经干细胞向神经元的分化 被引量:2
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作者 蔡闯 黄永辉 +4 位作者 曹兴兵 孙海涛 贯世豪 黄文康 韩博 《中国组织工程研究》 CAS 北大核心 2023年第24期3845-3851,共7页
背景:神经干细胞具有向神经元分化的潜能,已有报道表示星形胶质细胞与神经元存在密切联系,而有关星形胶质细胞来源细胞外囊泡是否具有促进神经干细胞向神经元高效分化的作用,仍未见相关报道。目的:探索星形胶质细胞来源细胞外囊泡诱导... 背景:神经干细胞具有向神经元分化的潜能,已有报道表示星形胶质细胞与神经元存在密切联系,而有关星形胶质细胞来源细胞外囊泡是否具有促进神经干细胞向神经元高效分化的作用,仍未见相关报道。目的:探索星形胶质细胞来源细胞外囊泡诱导促进神经干细胞向神经元分化的可能性。方法:(1)体外培养大鼠神经干细胞及星形胶质细胞,行细胞形态及免疫荧光鉴定,进一步提取星形胶质细胞来源细胞外囊泡,并通过透射电镜、粒径分析、Western blot等方法进行鉴定;(2)将第3代神经干细胞分2组,分别加入同体积的PBS、星形胶质细胞来源细胞外囊泡(质量浓度为1 mg/mL)干预6 d;(3)通过免疫荧光、qRT-PCR和Western blot检测星形胶质细胞来源细胞外囊泡干预后神经元标志物的表达水平。结果与结论:(1)显微镜下神经干细胞形态为球形,以细胞团状分布,免疫荧光示特征性标志物CD133、Nestin呈高表达水平;(2)显微镜下星形胶质细胞形态为不规则星状,且免疫荧光示特征性标志物胶质纤维酸性蛋白呈高表达水平;(3)星形胶质细胞来源细胞外囊泡形态近似圆形或椭圆形杯状,动态光粒子散射仪显示直径在10-200 nm之间,Western blot示细胞外囊泡相关标志物CD63及TSG101表达阳性;(4)相比PBS组,星形胶质细胞来源细胞外囊泡组可见神经干细胞大量贴壁分化,分化细胞胞体饱满,有明显突起,主要呈神经元样,且微管蛋白β3及神经丝蛋白200呈高表达(P<0.05);(5)以上结果提示,星形胶质细胞来源细胞外囊泡可促进神经干细胞向神经元分化。 展开更多
关键词 神经干细胞 星形胶质细胞 细胞外囊泡 中枢神经系统疾病 神经元 分化 脊髓损伤 神经修复
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