Aims: To screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships. Methods and Results: The bacteria distributed in air, surfaces and personnel in clean ro...Aims: To screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships. Methods and Results: The bacteria distributed in air, surfaces and personnel in clean rooms were routinely moni-tored using agar plates. Five isolates frequently isolated from the clean rooms of an aseptic pharmaceutical production workshop were selected based on their colony and cell morphology characteristics. Their physiological and biochemical properties, as well as partial 16S rDNA sequences, were analyzed. Results showed that all the five isolates belong to Gram positive bacteria, of which three were Staphylococcus, one Microbacterium and one Bacillus species. Sensitivity tests for these bacteria isolates to 3 disin-fectants showed that isolate F03 was obtuse, and had low susceptivity to UV irradiation, while isolates F02, F01 and F04 were not sensitive to phenol treatment. Isolates F04, F01 and F05 were resistant to chlorhexidine gluconate. Conclusion: Bacteria widely distributed in clean rooms are mainly a group of Gram positive strains, showing high resistance to selected disinfectants. Sig-nificance and impact of the study: Clean rooms are essential in aseptic pharmaceutical and food production. Screening bacteria isolates and identifying them is part of good manufacturing practices, and will aid in finding a more effective disinfection method.展开更多
Objective To analyze the factors that impact the environment quality of cleaning operating room and to discuss improvement measurement. Methods Environment bacteria testing results and prevalence of surgical incision ...Objective To analyze the factors that impact the environment quality of cleaning operating room and to discuss improvement measurement. Methods Environment bacteria testing results and prevalence of surgical incision infection in cleaning operating room were retro-展开更多
The contamination diffusion to the operating room when the door is open was simulated with a computational fluid dynamic(CFD) method,to give the extent of the contamination diffusion.The influence of the door-opening ...The contamination diffusion to the operating room when the door is open was simulated with a computational fluid dynamic(CFD) method,to give the extent of the contamination diffusion.The influence of the door-opening procedure was ignored since the door of the operating room is normally a sliding one.The flow field in the case of the 16 s course of opening the door was simulated.The simulated and the experimental results demonstrate that the extent of the contamination diffusion is around 1.5 m when there is no temperature difference between indoor and outdoor,and there is hardly any contamination diffusion when the temperature difference is 1 ℃.It can be concluded that the positive pressure difference in the operating room lost its function in preventing the contamination when the door is open.That the temperature of corridor is lower than that of operating room contributes to contamination control.Keeping 1 ℃ temperature difference between corridor and operating room and increasing positive pressure and air flow are suggested.It is more secure to set up an anteroom if persons come in or out of the operation room at the course of surgery.展开更多
重点知识园地重点单词1.mess n.杂乱;不整洁"What a mess!" she said after the party.聚会后她说:"真是一片狼藉!"短语(1)in a mess混乱;凌乱The room was in a mess.这个房间杂乱不堪。(2)make a mess弄脏;搞成...重点知识园地重点单词1.mess n.杂乱;不整洁"What a mess!" she said after the party.聚会后她说:"真是一片狼藉!"短语(1)in a mess混乱;凌乱The room was in a mess.这个房间杂乱不堪。(2)make a mess弄脏;搞成一团糟The kids made a mess in the living room.孩子们把客厅搞得一塌糊涂。展开更多
重点词汇1.fold v.折叠;对折Fold the card.折叠这张卡片。拓展unfold v.展开2.mess n.杂乱;不整洁The room was in a mess.这个房间杂乱不堪。'What a mess.'she said after the party.聚会后她说:'真是一片狼藉.'拓展...重点词汇1.fold v.折叠;对折Fold the card.折叠这张卡片。拓展unfold v.展开2.mess n.杂乱;不整洁The room was in a mess.这个房间杂乱不堪。'What a mess.'she said after the party.聚会后她说:'真是一片狼藉.'拓展in a mess混乱;凌乱make a mess弄脏;搞成一团糟The kids made a mess in the living room.孩子们把客厅搞得一塌糊涂。3.throw v.(threw)扔;掷Throw me the car keys.=Throw the car keys to me.把车钥匙扔给我。She threw her coat on the bed.她把外套扔在了床上。展开更多
基金Project (No. 30371703) supported by the National Natural ScienceFoundation of China
文摘Aims: To screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships. Methods and Results: The bacteria distributed in air, surfaces and personnel in clean rooms were routinely moni-tored using agar plates. Five isolates frequently isolated from the clean rooms of an aseptic pharmaceutical production workshop were selected based on their colony and cell morphology characteristics. Their physiological and biochemical properties, as well as partial 16S rDNA sequences, were analyzed. Results showed that all the five isolates belong to Gram positive bacteria, of which three were Staphylococcus, one Microbacterium and one Bacillus species. Sensitivity tests for these bacteria isolates to 3 disin-fectants showed that isolate F03 was obtuse, and had low susceptivity to UV irradiation, while isolates F02, F01 and F04 were not sensitive to phenol treatment. Isolates F04, F01 and F05 were resistant to chlorhexidine gluconate. Conclusion: Bacteria widely distributed in clean rooms are mainly a group of Gram positive strains, showing high resistance to selected disinfectants. Sig-nificance and impact of the study: Clean rooms are essential in aseptic pharmaceutical and food production. Screening bacteria isolates and identifying them is part of good manufacturing practices, and will aid in finding a more effective disinfection method.
文摘Objective To analyze the factors that impact the environment quality of cleaning operating room and to discuss improvement measurement. Methods Environment bacteria testing results and prevalence of surgical incision infection in cleaning operating room were retro-
文摘The contamination diffusion to the operating room when the door is open was simulated with a computational fluid dynamic(CFD) method,to give the extent of the contamination diffusion.The influence of the door-opening procedure was ignored since the door of the operating room is normally a sliding one.The flow field in the case of the 16 s course of opening the door was simulated.The simulated and the experimental results demonstrate that the extent of the contamination diffusion is around 1.5 m when there is no temperature difference between indoor and outdoor,and there is hardly any contamination diffusion when the temperature difference is 1 ℃.It can be concluded that the positive pressure difference in the operating room lost its function in preventing the contamination when the door is open.That the temperature of corridor is lower than that of operating room contributes to contamination control.Keeping 1 ℃ temperature difference between corridor and operating room and increasing positive pressure and air flow are suggested.It is more secure to set up an anteroom if persons come in or out of the operation room at the course of surgery.
文摘重点知识园地重点单词1.mess n.杂乱;不整洁"What a mess!" she said after the party.聚会后她说:"真是一片狼藉!"短语(1)in a mess混乱;凌乱The room was in a mess.这个房间杂乱不堪。(2)make a mess弄脏;搞成一团糟The kids made a mess in the living room.孩子们把客厅搞得一塌糊涂。
文摘重点词汇1.fold v.折叠;对折Fold the card.折叠这张卡片。拓展unfold v.展开2.mess n.杂乱;不整洁The room was in a mess.这个房间杂乱不堪。'What a mess.'she said after the party.聚会后她说:'真是一片狼藉.'拓展in a mess混乱;凌乱make a mess弄脏;搞成一团糟The kids made a mess in the living room.孩子们把客厅搞得一塌糊涂。3.throw v.(threw)扔;掷Throw me the car keys.=Throw the car keys to me.把车钥匙扔给我。She threw her coat on the bed.她把外套扔在了床上。
