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Effect of Extending Voluntary Waiting Period on Health of Holstein Friesian Dairy Cows
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作者 Găvan Constantin Șonea Cosmin 《Open Journal of Veterinary Medicine》 CAS 2024年第6期124-136,共13页
The aim of this of this experimental study to investigate the effect of 3 voluntary waiting periods (VWP) on health of dairy cows. A total of 100 lactations of 100 Holstein Friesian cows with high milk production (ave... The aim of this of this experimental study to investigate the effect of 3 voluntary waiting periods (VWP) on health of dairy cows. A total of 100 lactations of 100 Holstein Friesian cows with high milk production (average 9.442 ± 620 kg) were randomly (based on the ear tags digits of cows) distributed to 1 to 3 experimental groups with VWP of 50 (VWPG50;n = 32), 100 (VWPG100;n = 34) or 150 days (VWPG150;n = 34). Observations relating to diseases of the cows were made by the farm veterinarian and research personnel. Were defined eight disease: puerperal fever, retained fetal membranes, displaced abomasum, metritis, clinical ketosis, clinical mastitis, ovarian cysts, and claw diseases. The experimental days (ED) were from 4 days in milk (DIM) to 100 days after the next calving. The experiment was managed at Dairy Research Farm ?imnic-Craiova January 2018 to December 2022. A clear set of clinical signs were used to define a case at the diseases without the need for laboratory confirmation. Values of Lactational incidence risk (LIR) median postpartum days at diagnosis and pairwise comparison of proportion between VWPs were calculated. LIR for puerperal fiver, retained fetal membranes, displaced abomasum, clinical ketosis, clinical mastitis, ovarian cysts and claw disease were 4;5;2;8;8;and 4% respectively. Numerically, cows with VWP of 100 and150 days had more disease cases compared with VWP of 50 days. The differences between experimental groups of cows regarding proportions of cows with disease cases were not statistically significant. Any of the eight disorders reported in this study was analyzed independently to other health problems. For all enrolled cows (n = 100) VWP was extended until 100 or 150 days postpartum with no effect on the lactational incidence risk for the eight disorders analyzed. 展开更多
关键词 Voluntary Waiting Period Dairy cow Health Lactation Disorders Lactation Incidence Risk for cow Diseases
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Maternal supplementation with n-3 fatty acids affects placental lipid metabolism, inflammation, oxidative stress, the endocannabinoid system, and the neonate cytokine concentrations in dairy cows
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作者 Priscila dos Santos Silva Gitit Kra +3 位作者 Yana Butenko Jayasimha Rayalu Daddam Yishai Levin Maya Zachut 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期2012-2030,共19页
Background The placenta plays a crucial role in supporting and influencing fetal development.We compared the effects of prepartum supplementation with omega-3(n-3)fatty acid(FA)sources,flaxseed oil(FLX)and fish oil(FO... Background The placenta plays a crucial role in supporting and influencing fetal development.We compared the effects of prepartum supplementation with omega-3(n-3)fatty acid(FA)sources,flaxseed oil(FLX)and fish oil(FO),on the expression of genes and proteins related to lipid metabolism,inflammation,oxidative stress,and the endocannabinoid system(ECS)in the expelled placenta,as well as on FA profile and inflammatory response of neonates.Late-pregnant Holstein dairy cows were supplemented with saturated fat(CTL),FLX,or FO.Placental cotyledons(n=5)were collected immediately after expulsion,and extracted RNA and proteins were analyzed by RTPCR and proteomic analysis.Neonatal blood was assessed for FA composition and concentrations of inflammatory markers.Results FO increased the gene expression of fatty acid binding protein 4(FABP4),interleukin 10(IL-10),catalase(CAT),cannabinoid receptor 1(CNR1),and cannabinoid receptor 2(CNR2)compared with CTL placenta.Gene expression of ECS-enzyme FA-amide hydrolase(FAAH)was lower in FLX and FO than in CTL.Proteomic analysis identified 3,974 proteins;of these,51–59 were differentially abundant between treatments(P≤0.05,|fold change|≥1.5).Top canonical pathways enriched in FLX vs.CTL and in FO vs.CTL were triglyceride metabolism and inflammatory processes.Both n-3 FA increased the placental abundance of FA binding proteins(FABPs)3 and 7.The abundance of CNR1 cannabinoid-receptor-interacting-protein-1(CNRIP1)was reduced in FO vs.FLX.In silico modeling affirmed that bovine FABPs bind to endocannabinoids.The FLX increased the abundance of inflammatory CD44-antigen and secreted-phosphoprotein-1,whereas prostaglandin-endoperoxide synthase 2 was decreased in FO vs.CTL placenta.Maternal FO enriched neonatal plasma with n-3 FAs,and both FLX and FO reduced interleukin-6 concentrations compared with CTL.Conclusion Maternal n-3 FA from FLX and FO differentially affected the bovine placenta;both enhanced lipid metabolism and modulated oxidative stress,however,FO increased some transcriptional ECS components,possibly related to the increased FABPs.Maternal FO induced a unique balance of pro-and anti-inflammatory components in the placenta.Taken together,different sources of n-3 FA during late pregnancy enhanced placental immune and metabolic processes,which may affect the neonatal immune system. 展开更多
关键词 ANTIOXIDANTS Dairy cows Endocannabinoid system INFLAMMATION Omega-3 fatty acids PLACENTA
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Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows
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作者 Fanlin Kong Feiran Wang +3 位作者 Yijia Zhang Shuo Wang Wei Wang Shengli Li 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第2期795-811,共17页
Background The dairy cow’s postpartum period is characterized by dramatic physiological changes,therefore imposing severe challenges on the animal for maintaining health and milk output.The dynamics of the ruminal mi... Background The dairy cow’s postpartum period is characterized by dramatic physiological changes,therefore imposing severe challenges on the animal for maintaining health and milk output.The dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation launch.We aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation(RMT)and sterile RMT in postpartum dairy cows.Twelve fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid collection.Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid(FR),10 L sterile rumen fluid(SR),or 10 L saline(CON)during 3 d after calving.Results Production performance,plasma indices,plasma lipidome,ruminal microbiome,and liver transcriptome were recorded.After fresh and sterile RMT,we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group.A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40%or 47.85%,whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34%or 66.85%.Dry matter intake and feed efficiency were not affected by treatments.Plasmaβ-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty acids.Hepatic transcriptomics analysis indicated acutephase response pathways were upregulated in the SR group.Conclusions Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed efficiency.Inoculation with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows. 展开更多
关键词 Dairy cow LIPIDOMICS Liver Rumen microbiota transplantation Transition period
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Spatially resolved transcriptomic profiling of placental development in dairy cow
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作者 Guang-Hui Tan Shi-Jie Liu +11 位作者 Ming-Le Dou De-Feng Zhao Ao Zhang Heng-Kuan Li Fu-Nong Luo Tao Shi Hao-Ping Wang Jing-Yuan Lei Yong Zhang Yu Jiang Yi Zheng Fei Wang 《Zoological Research》 SCIE CSCD 2024年第3期586-600,共15页
The placenta plays a crucial role in successful mammalian reproduction.Ruminant animals possess a semi-invasive placenta characterized by a highly vascularized structure formed by maternal endometrial caruncles and fe... The placenta plays a crucial role in successful mammalian reproduction.Ruminant animals possess a semi-invasive placenta characterized by a highly vascularized structure formed by maternal endometrial caruncles and fetal placental cotyledons,essential for full-term fetal development.The cow placenta harbors at least two trophoblast cell populations:uninucleate(UNC)and binucleate(BNC)cells.However,the limited capacity to elucidate the transcriptomic dynamics of the placental natural environment has resulted in a poor understanding of both the molecular and cellular interactions between trophoblast cells and niches,and the molecular mechanisms governing trophoblast differentiation and functionalization.To fill this knowledge gap,we employed Stereo-seq to map spatial gene expression patterns at near single-cell resolution in the cow placenta at 90 and 130 days of gestation,attaining high-resolution,spatially resolved gene expression profiles.Based on clustering and cell marker gene expression analyses,key transcription factors,including YBX1 and NPAS2,were shown to regulate the heterogeneity of trophoblast cell subpopulations.Cell communication and trajectory analysis provided a framework for understanding cell-cell interactions and the differentiation of trophoblasts into BNCs in the placental microenvironment.Differential analysis of cell trajectories identified a set of genes involved in regulation of trophoblast differentiation.Additionally,spatial modules and co-variant genes that help shape specific tissue structures were identified.Together,these findings provide foundational insights into important biological pathways critical to the placental development and function in cows. 展开更多
关键词 Spatial transcriptomics Dairy cow PLACENTA GESTATION
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Abomasal infusion of branched-chain amino acids or branched-chain keto-acids alter lactation performance and liver triglycerides in fresh cows
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作者 Kristen Gallagher Isabelle Bernstein +7 位作者 Cynthia Collings David Main Ghayyoor Ahmad Sarah Naughton Jayasimha Daddam Vengai Mavangira Mike Vandehaar Zheng Zhou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第3期1145-1157,共13页
Background Dairy cows are at high risk of fatty liver disease in early lactation,but current preventative measures are not always effective.Cows with fatty liver have lower circulating branched-chain amino acid(BCAA)c... Background Dairy cows are at high risk of fatty liver disease in early lactation,but current preventative measures are not always effective.Cows with fatty liver have lower circulating branched-chain amino acid(BCAA)concentra-tions whereas cows with high circulating BCAA levels have low liver triglyceride(TG).Our objective was to determine the impact of BCAA and their corresponding ketoacids(branched-chain ketoacids,BCKA)on production performance and liver TG accumulation in Holstein cows in the first 3 weeks postpartum.Methods Thirty-six multiparous Holstein cows were used in a randomized block design experiment.Cows were abomasally infused for the first 21 d postpartum with solutions of 1)saline(CON,n=12);2)BCA(67 g valine,50 g leu-cine,and 34 g isoleucine,n=12);and 3)BCK(77 g 2-ketovaline calcium salt,57 g 2-ketoleucine calcium salt,and 39 g 2-ketoisoleucine calcium salt,n=12).All cows received the same diet.Treatment effects were determined using PROC GLIMMIX in SAS.Results No differences were detected for body weight,body condition score,or dry matter intake averaged over the first 21 d postpartum.Cows receiving BCK had significantly lower liver TG concentrations compared to CON(6.60%vs.4.77%,standard error of the mean(SEM)0.49)during the first 3 weeks of lactation.Infusion of BCA increased milk yield(39.5 vs.35.3 kg/d,SEM 1.8),milk fat yield(2.10 vs.1.69 kg/d,SEM 0.08),and lactose yield(2.11 vs.1.67 kg/d,SEM 0.07)compared with CON.Compared to CON,cows receiving BCA had lower plasma glucose(55.0 vs.59.2 mg/dL,SEM 0.86)but higherβ-hydroxybutyrate(9.17 vs.6.00 mg/dL,SEM 0.80).Conclusions Overall,BCAA supplementation in this study improved milk production,whereas BCKA supplementa-tion reduced TG accumulation in the liver of fresh cows. 展开更多
关键词 Branched-chain amino acids Branched-chain ketoacids Fatty liver Fresh cow
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Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3
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作者 ZHANG Yu LI Xiangting +4 位作者 SUN Yalin XUE Aidi ZHANG Yi JIANG Hailong SHEN Weizheng 《智慧农业(中英文)》 CSCD 2024年第4期29-41,共13页
[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been propo... [Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings. 展开更多
关键词 cow rumination behavior real-time monitoring edge computing improved MobileNet v3 edge intelligence model Bi-LSTM
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Feeding Calcium Salts of Linseed Oil on Metabolic Status and Reproductive Traits in Grazing Dairy Cows
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作者 Jesica Daniela Iorio Yaliska Moreno González +6 位作者 Maria Florencia Olmeda Dino Curletto Daniel Edgardo Scandolo Martin Guillermo Maciel Rafael Alejandro Palladino Mauricio Javier Giuliodori Eloy Eduardo Salado 《Open Journal of Animal Sciences》 2024年第3期219-233,共15页
This experiment aimed to evaluate the effect of calcium salts of linseed oil (rich in alpha-linolenic acid (ALA, C18:3n-3) on metabolic and reproductive traits in high-producing dairy cows under grazing. Thirty-six Ho... This experiment aimed to evaluate the effect of calcium salts of linseed oil (rich in alpha-linolenic acid (ALA, C18:3n-3) on metabolic and reproductive traits in high-producing dairy cows under grazing. Thirty-six Holstein dairy cows were randomly assigned, in a complete block design, to receive ALA supplementation (0.85 kg∙day−1 of calcium salts of linseed oil) or to remain as untreated control (CON). The concentrate was formulated to offer the same amount of energy across treatments (CON cows received an extra kg of corn to compensate for the higher energy density of ALA treatment). A PMR + Alfalfa pasture was offered to all cows at the same time. A fixed time artificial insemination (FTAI) at 80 DIM, preceded by a Presynch plus Ovsynch protocol was implemented for the first service and later, on return to estrus, heat detection and artificial insemination (AI) were performed. Pregnancy diagnosis was checked at 30, 42, 60, and 90 d after AI. Blood and milk samples were taken biweekly. Treatment affected plasma cholesterol concentration (160.36 vs. 186.70 mg∙dl−1, p = 0.03, for ALA and CON, respectively) and on size of corpus luteum (CL, 17.6 vs. 13.7 mm, p = 0.02, for ALA and CON, respectively). Supplementation tended (p = 0.136) to increase conception rate by 200 DIM (81.69% vs. 55.43% in ALA and CON cows, respectively). However, treatment had no effect (p > 0.05) on body weight (BW), body condition score (BCS), and circulating levels of beta-hydroxybutyrate (BHBA), glucose, insulin, growth hormone (GH) and insulin-like growth factor (IGF-I). Our results suggest that supplementation with calcium salts of linseed oil could enhance ovarian function without affecting energy metabolism in early lactation dairy cows. 展开更多
关键词 α-Linolenic Acid Supplementation REPRODUCTION GRAZING Dairy cows
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A Biogas Production Model from the Combination of Pig Manure and Cow Dung in N’Zérékoré City, Republic of Guine
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作者 Madeleine Kamano Oumar Keita +1 位作者 Ansoumane Sakouvogui Aboubacar Sangare 《Energy and Power Engineering》 2024年第9期293-312,共20页
This present research work focuses on the valorization of pig droppings for production of biogas in mono digestion and co-digestion with proportions of cow dung from the urban commune of N’Zérékoré. It... This present research work focuses on the valorization of pig droppings for production of biogas in mono digestion and co-digestion with proportions of cow dung from the urban commune of N’Zérékoré. It was carried out in December 2020 in the Physics laboratory of the University of N’Zérékoré. The anaerobic digestion process took 25 days in an almost constant ambient temperature of 25˚C. Five digesters were loaded on 12/06/2020, two of which with 1 kg of pig dung and 1 kg of cow dung both in mono-digestion. The 3 other digesters in co-digestion with different proportions of pig manure and cow dung. The substrate in each digester is diluted in 2 liters of water, with a proportion of (1/2). The main results obtained are: 1) the evolution of the temperature and pH during digestion process, 2) the average biogas productions 0.61 liters for (D1);1.20 liter for (D2);1.65 liter for (D3);1.51 liter for (D4) and 1.31 liter for (D5). The cumulative amounts of biogas are respectively: D1 (7.95 liters), D2 (15.60 liters), D3 (21.50 liters), D4 (19.65 liters) and D5 (17.05 liters). The total cumulative production is 81.75 liters at the end of the process. The originality of this research work is that the proposed model examines the relation between the daily biogas production and the variation of temperature, pH and pressure. The combustibility test showed the biogas produced during the first week was no combustible (contains less than 50% methane). Combustion started from the biogas produced from the 15th day and it is from the 20th day that a significant amount of stable yellow/blue flame was observed. The results of this study show the combination of pig manure and cow dung presents advantages for optimal biogas production. 展开更多
关键词 Production Experimental MODEL Pig Manure cow Dung BIOGAS N’Zérékoré Republic of Guinea
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脉冲电沉积CoW/ZrO_(2)复合镀层的力学性能研究
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作者 张翠杰 王文龙 赵敬云 《河南工学院学报》 CAS 2024年第2期27-31,39,共6页
研究了镀液中ZrO_(2)纳米颗粒添加量对COW/ZrO_(2)复合镀层质量和摩擦磨损性能的影响。采用单因素试验法,在一定条件下改变镀液中ZrO_(2)纳米颗粒的添加量从而获得不同的COW/ZrO_(2)复合镀层。用扫描电子显微镜SU5000观察镀层表面形貌,... 研究了镀液中ZrO_(2)纳米颗粒添加量对COW/ZrO_(2)复合镀层质量和摩擦磨损性能的影响。采用单因素试验法,在一定条件下改变镀液中ZrO_(2)纳米颗粒的添加量从而获得不同的COW/ZrO_(2)复合镀层。用扫描电子显微镜SU5000观察镀层表面形貌,用X射线衍射和表面分析仪P7分别检测镀层的微观结构、厚度和表面粗糙度,研究镀层表面质量随镀液中ZrO_(2)纳米颗粒添加量的变化规律并测试镀层的摩擦学性能。试验结果表明:在一定条件下镀液中ZrO_(2)纳米颗粒的添加量对复合镀层质量的影响存在最优值,镀液中ZrO_(2)含量的变化对于镀层磨损率的减小存在最优值。采用脉冲电沉积法制备COW/ZrO_(2)复合镀层的过程中,ZrO_(2)纳米颗粒添加量为9g/L时复合镀层的表面质量和耐磨性最好。 展开更多
关键词 脉冲电沉积 cow/ZrO_(2) 复合镀层 摩擦磨损性能
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Study of the Optimal Conditions for Anaerobic Digestion of Cow Dung in Households in Cote d’Ivoire
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作者 Sahi Louh Theophile Roch Ori +1 位作者 Kamenan Blaise Koua Prosper Gbaha 《Open Journal of Applied Sciences》 2024年第9期2679-2696,共18页
This paper investigates the optimal conditions for methanisation applied to cow dung. Four experimental 25 liters digesters were used in this work. The best biogas yield is obtained when the digester is installed in a... This paper investigates the optimal conditions for methanisation applied to cow dung. Four experimental 25 liters digesters were used in this work. The best biogas yield is obtained when the digester is installed in a metal box and exposed to sunlight. The temperature in this digester varied between 25˚C and 37˚C. The dry matter content of the collected cow dung was 15.5%. The digester was fed with 9 kg of cow dung mixed with 8.5 litres of water, one litre of cassava effluent and 200 ml of human urine. After a retention period of 22 days, the biogas obtained was 67% methane and 21% carbon dioxide. The use of human urine and cassava effluent improved the quality of the biogas. 展开更多
关键词 Methanisation Cassava Effluent Human Urine cow Dung BIOGAS
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Chemical Analysis of Activated Carbon from Bull and Cow Horns Pyrolysis to Be Used as Antidotes
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作者 Alexandre Ngama Mwabi Pierre Yoniene Yassa Vestine Ntakarutimana 《Open Journal of Applied Sciences》 2024年第8期2133-2143,共11页
The purpose of this study is to compare the results of chemical analysis of two types of activated from the pyrolysis of bull horn and that of cow. Six samples were used to measure pH, carbon, calcium and to determine... The purpose of this study is to compare the results of chemical analysis of two types of activated from the pyrolysis of bull horn and that of cow. Six samples were used to measure pH, carbon, calcium and to determine adsorbent power. The pH was measured at a temperature of 20˚C using an “ANION 7010 ionomer” pH meter, the carbon (C) content was analyzed using a “EURO EA 3000” analyzer. and the electronic balance: “Sartorius CP-2P”, calcium (Ca) was analyzed using a DFS-8 spectrograph. For the adsorbency test, the 0.15% methylene blue R solution was used. At the end of this study, we found that the activated carbon from the bull horn demonstrated a carbon content that is higher than that of the cow horn (20.79% against 15.63%), activated carbon of cow horn is richer in calcium than that of bull horn (16.27% against 3.69%) and then the pH. The cow horn is higher than that of the bull horn (7.43 versus 6.5). For the adsorbent power, the sample (75% bull horn and 25% cow horn) was recorded with the greatest adsorbent power. Thus, from this study, it can be recommended as an activated carbon antidote to be used for poisonings treatment. 展开更多
关键词 Activated Carbon Bull Horn cow Horn Oil Palm Nut Shells Absorbent Power
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Dairy cows inoculated with highly pathogenic avian influenza virus H5N1
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作者 Amy L Baker 《四川生理科学杂志》 2024年第10期2275-2275,共1页
Highly pathogenic avian influenza(HPAI)H5N1 hemagglutinin clade 2.3.4.4b was detected in the United States in 2021.These HPAI viruses caused mortality events in poultry,wild birds,and wild mammals.On March 25,2024,HPA... Highly pathogenic avian influenza(HPAI)H5N1 hemagglutinin clade 2.3.4.4b was detected in the United States in 2021.These HPAI viruses caused mortality events in poultry,wild birds,and wild mammals.On March 25,2024,HPAI H5N1 clade 2.3.4.4b was confirmed in a dairy cow in Texas in response to a multi-state investigation into milk production losses.1 Over 200 positive herds were identified in 14 U.S.states.The case description included reduced feed intake and rumen motility in lactating cows,decreased milk production,and thick yellow milk.2,3 The diagnostic investigation revealed viral RNA in milk and mammary tissue with alveolar epithelial degeneration and necrosis and positive immunoreactivity of glandular epithelium.A single transmission event,likely from birds,was followed by limited local transmission and onward horizontal transmission of H5N1 clade 2.3.4.4b genotype B3.13.4 We sought to experimentally reproduce infection with genotype B3.13 in Holstein yearling heifers and lactating cows.Heifers were inoculated by aerosol respiratory route and cows by intramammary route.Clinical disease was mild in heifers,but infection was confirmed by virus detection,lesions,and seroconversion.Clinical disease in lactating cows included decreased rumen motility,changes to milk appearance,and production losses.Infection was confirmed by high levels of viral RNA detected in milk,virus isolation,lesions in mammary tissue,and seroconversion.This study provides the foundation to investigate additional routes of infection,pathogenesis,transmission,and intervention strategies. 展开更多
关键词 H5N1 cowS FIR
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基于优化的COW虚拟块设备的虚拟机按需部署机制 被引量:9
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作者 陈彬 肖侬 +1 位作者 蔡志平 王志英 《计算机学报》 EI CSCD 北大核心 2009年第10期1915-1926,共12页
基于COW(Copy-on-Write)读写模式的虚拟块设备有利于实现大规模虚拟机环境下虚拟机的快速部署.文中为虚拟机管理器中的COW虚拟块设备设计了一种优化方法,能够提高COW磁盘的访问性能以及生成多个小尺寸的COW磁盘映像文件,以降低通过网络... 基于COW(Copy-on-Write)读写模式的虚拟块设备有利于实现大规模虚拟机环境下虚拟机的快速部署.文中为虚拟机管理器中的COW虚拟块设备设计了一种优化方法,能够提高COW磁盘的访问性能以及生成多个小尺寸的COW磁盘映像文件,以降低通过网络部署虚拟机的开销.基于优化的COW虚拟块设备,文中提出了虚拟机环境下的虚拟机按需部署机制及关键技术问题的解决方案.基于Linux平台和QEMU虚拟机,实现了基于优化COW虚拟块设备的虚拟机按需部署原型系统.实验表明,优化的COW虚拟块设备、基于COW磁盘有效工作集的优化部署以及COW磁盘回收等方法能够有效地支持虚拟机环境下低开销的、按需的虚拟机部署. 展开更多
关键词 虚拟机 虚拟机管理器 虚拟机按需部署 cow磁盘 有效工作集
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Study on the Effects of Chinese Herb Additive on Milk Yield and Anti-heat Stress Ability of Holstein Cow 被引量:21
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作者 赵政 陈学文 李仕坚 《Agricultural Science & Technology》 CAS 2008年第3期111-114,共4页
[Objective] The research aimed to explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein cow.[Method] 36 Holstein cows with similar birth order and milk yield were equally ... [Objective] The research aimed to explore the effects of Chinese herb additive on milk yield and anti-heat stress ability of Holstein cow.[Method] 36 Holstein cows with similar birth order and milk yield were equally and randomly divided into 3 groups which were control group, Chinese medicine Ⅰ group and Chinese medicine Ⅱ group. In control group, cows were fed with basic feed, while in Chinese medicine Ⅰ group, cows were fed with basic feed containing 4% compound Chinese herb additive and in Chinese medicine Ⅱ group, cows were fed with basic feed containing 3% compound Chinese herb additive.All cows in the 3 groups were analyzed comparatively.[Result] Adding some compound Chinese herb additive into basic feed would increase milk yield and anti-heat stress ability of Holstein cow,besides,it also could decrease cow diseases such as cow mastitis.Chinese medicine Ⅰ would generate the highest economic benefit of practical production.[Conclusion] Adding Chinese herb additive into basic feed is an effective way for reliefing heat stress and increasing milk yield. 展开更多
关键词 Chinese HERB ADDITIVE HOLSTEIN cow Milk yield Anti-heat stress
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油罐机械清洗设备COW在我国的首次应用 被引量:10
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作者 蒋连生 戴良 +2 位作者 徐峰 贺立平 马成山 《油气储运》 CAS 北大核心 1998年第2期37-41,共5页
对引进日本的机械清洗设备COW,在东营首站第一次使用的情况做了较详细的介绍。该项技术在油罐的清洗中具有机械化程度高、施工周期短、安全可靠、原油回收率高、不会对环境造成污染、综合效益高等优点。同时介绍了COW设备的组成及清洗... 对引进日本的机械清洗设备COW,在东营首站第一次使用的情况做了较详细的介绍。该项技术在油罐的清洗中具有机械化程度高、施工周期短、安全可靠、原油回收率高、不会对环境造成污染、综合效益高等优点。同时介绍了COW设备的组成及清洗施工现场的布置情况,并指出了在今后清洗作业中应注意的事项。 展开更多
关键词 油罐 cow 机械清洗设备 维修
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Effects of Enzyme on Material Transformation of Cow Dung Compost 被引量:7
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作者 任平 徐升运 +1 位作者 阮祥稳 赵文娟 《Agricultural Science & Technology》 CAS 2010年第3期143-146,共4页
[Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperatur... [Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperature,moisture,pH value,crude fiber,TOC,TN,GI during composting.[Result] The results showed that adding complex enzymes could accelerate degradation of organic matter in pre-composting period.Crude fiber of 2.0%,.1.5% enzyme treatment group and control group decreased 49.6%,47% and 29.1% respectively,TOC decreased 41.7%,35.3% and 21.1%,TN decreased 32.6%,26.8% and 19.2%.2.0%,1.5% enzyme treatment groups could reach basic maturity degree at 30 d.[Conclusion] Composting cycle be shortened by adding complex enzymes,which was useful for maturity of cow dung compost. 展开更多
关键词 ENZYME cow dung COMPOST
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Effects of Herbal Feed Additive on Milk Production of Dairy Cows 被引量:5
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作者 高桂生 高光平 +4 位作者 李正本 史秋梅 张艳英 邵新华 梁银聚 《Agricultural Science & Technology》 CAS 2013年第10期1462-1464,共3页
[Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided... [Objective] The aim of this study was to investigate the efficacy of the herbal feed additive Zengrujianniusan on the milk production of dairy cows. [Method] Thirty-two black-white lactating cows were randomly divided into four groups, and were fed with forage supplemented with 0 (control group), 0.2%, 0.4% and 0.6% Zengrujianniusan for 60 d. During this period, the contents of fat, protein and non-fat solid in milk were measured every 20 d. [Result] The milk production of the cows whose forage was added with 0.2 %, 0.4 % and 0.6% Zengrujianniusan was 4.02%, 12.50%, 14.00% higher than that of the control (P〉0.05). The herbal feed additive had no significant influence on the contents of fat, milk and non-fat solid in milk, but significantly reduced the number of somatic cells. [Conclusion] The study will provide reference for developing feed additives which is safe and non-toxic to cows and their milk. 展开更多
关键词 Herbal feed additive Lactating cows Milk production
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CoW_(11)Ti/淀粉纳米复合物的合成与表征 被引量:2
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作者 王晓红 王立敏 +1 位作者 李凤 刘景福 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2003年第12期2158-2160,共3页
Using a reversed-phase microemulsion polymerization method, polyoxometalates(POMs) CoW 11Ti loaded starch nanoparticles were prepared and structurally characterized by elemental analyses, IR, UV-Vis and ESR spectrosco... Using a reversed-phase microemulsion polymerization method, polyoxometalates(POMs) CoW 11Ti loaded starch nanoparticles were prepared and structurally characterized by elemental analyses, IR, UV-Vis and ESR spectroscopy. The particle size of CoW 11Ti/starch was estimated by transmission electron microscope(TEM) and the size ranges by a 1000HSA MALVIRN Zetasizer instrument. The result shows that the polyoxometalate retained the parent structure after encapsulated by starch microspheres, which are able to enhance the stability and antitumoral activity of POMs and decrease the toxicity of POMs as well. 展开更多
关键词 多酸化合物 K6H2[cow11TiO40]·12H2O 淀粉纳米粒子 载药体系 合成 表征 抗HIV病毒活性 抗肿瘤活性
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K_5CoW_(12)O_(40)·3H_2O催化合成苯甲醛1,2-丙二醇缩醛 被引量:5
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作者 赖君玲 刘春生 +1 位作者 罗根祥 沈飞 《辽宁石油化工大学学报》 CAS 2010年第2期1-3,共3页
研究了K5CoW12O40.3H2O催化剂对苯甲醛和1,2-丙二醇为原料合成苯甲醛1,2-丙二醇缩醛的反应的催化活性。考察了醛醇物质的量比、催化剂质量、带水剂体积、反应时间、催化剂重复使用性等因素对产品收率的影响。结果表明,K5CoW12O40.3H2O... 研究了K5CoW12O40.3H2O催化剂对苯甲醛和1,2-丙二醇为原料合成苯甲醛1,2-丙二醇缩醛的反应的催化活性。考察了醛醇物质的量比、催化剂质量、带水剂体积、反应时间、催化剂重复使用性等因素对产品收率的影响。结果表明,K5CoW12O40.3H2O是缩醛反应的良好催化剂。在最佳实验条件下:苯甲醛与1,2-丙二醇物质的量比为1:1.2、苯甲醛物质的量为0.1mol、催化剂质量为0.3g、环己烷体积为10mL、反应时间45min,苯甲醛1,2-丙二醇缩醛的收率最高可达92.8%。 展开更多
关键词 苯甲醛l 2-丙二醇缩醛 K5cow12O40·3H2O 催化 缩醛反应
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并行多种群自适应遗传算法在COW集群上的实现 被引量:1
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作者 刘虎 张焕春 +1 位作者 经亚枝 朱力立 《南京航空航天大学学报》 EI CAS CSCD 北大核心 2005年第6期760-765,共6页
为改善标准遗传算法的求解效率,提出一种基于6模糊控制器的并行多种群自适应遗传算法。利用MPI(Messagepassinginterface)技术建立了一个COW(Clusterofworkstation)集群,将算法在该硬件平台上进行了实现。3机COW集群的仿真实验结果在演... 为改善标准遗传算法的求解效率,提出一种基于6模糊控制器的并行多种群自适应遗传算法。利用MPI(Messagepassinginterface)技术建立了一个COW(Clusterofworkstation)集群,将算法在该硬件平台上进行了实现。3机COW集群的仿真实验结果在演示算法设计可行性的同时,表明该算法的求解效率明显优于用于对照的单种群算法,具有在解决组合优化问题上广泛应用的可能。本文还对影响并行算法的参数进行了探讨。 展开更多
关键词 并行遗传算法 自适应参数控制 cow集群 MPI
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