Field Control Effects and Crop Safety Assessment of Triazole Fungicides on Apple Rust

In order to provide a reference basis for efficient prevention and control of apple rust,apple rust(Gymnosporangium yamadai)was used as the test material,the field control effects of six triazole fungicides with different concentrations and different application times on apple rust,and the safety of application in different periods on the crop were investigated in field trials.The results showed that the control effect and safety of myclobutanil were excellent.In the prevention test,the effects of myclobutanil 40%EC 2000 times and 4000 times dilutions in protecting apple leaves from G.yamadai infection reached more than 98%at 10 d after the second spraying in the fruitlet stage,and in the treatment test,the control effects of inhibiting the spread of leaf lesions were 90.89%and 87.17%at 16 d after the fourth spraying in the fruit expansion stage,respectively.The results of the crop safety test showed that when applying the triazole fungicides with different concentrations from the red bud stage to the fruit expansion stage of apple trees,there were no adverse effects on the leaves,branches,flowers and fruit,as well as no rust or deformity on the fruit surface.When spraying the fungicides from the red bud stage to the fruit expansion stage,except that the single fruit weights in the treatment of the 10%difenoconazole DC 2000 times and myclobutanil 40%EC 4000 times dilutions were reduced without a significant difference from the control,and the values of other treatments were all reduced by more than 9%compared with the control.When applying the fungicides during the fruit expansion period,none of the tested fungicide treatments had a significant effect on the fruit growth.

Study on Weed Control and Safety of Tembotrione-Atrazine Tank Mixture in Spring Maize Fields

This study aimed to explore the effect of 5% tembotrione oil dispersion(OD)-38% atrazine suspension concentrate(SC) tank mixture on the weeds in spring maize fields and the safety of the mixture. To be specific, randomized block design was adopted for the field experiments. The results showed that the application of tankmixture of 5% tembotrione OD at 90 g a.i./hm^(2)and 38% atrazine SC at 570 g a.i./hm^(2)and the mixture of 5% tembotrione OD at 90 g a.i./hm^(2)and 38% atrazine SC at 1140 g a.i./hm^(2)respectively controlled 94.7%~96.3% and 94.0%~96.3% of the grass weeds, 94.7%~96.0% and 93.7%~95.7% fresh weight of grass weeds, 95.3%~96.3%and 93.3%~97.3% of broadleaf weeds, and 94.7%~96.0% and 93.3%~96.3% fresh weight of broadleaf weeds. The efficacy was better than that of the 5% tembotrione alone and the control herbicide and no phytotoxicity of the mixtures was identified.The tank mixture of tembotrione and atrazine should be promoted in the spring maize field for weed control and the recommended dosages were 90 g a.i./hm^(2)for 5%tembotrione and 570 g a.i./hm^(2)for 38% atrazine.

Herbicidal activity of Aureobasidium pullulans PA-2 on weeds and optimization of its solid-state fermentation conditions

Fungal strain PA-2 was isolated from infected poplar leaves from the Ping’an District, Haidong City of Qinghai Province, China. Based on the culture characteristics and the internal transcribed spacer(ITS) sequence of its 16S r DNA, the strain was identified as Aureobasidium pullulans. The culture and metabolites of strain PA-2 showed high herbicidal potential to five tested weeds Galium aparine var. tenerum, Chenopodium album, Malva crispa, Polygonum lapathifolium and Avena fatua. For the in vitro test, 5 days after the detached leaves were inoculated with PA-2 culture, all leaves infected by the hyphae and became black and rotten. For the in vivo test, a metabolite filtrate of PA-2 culture was sprayed over the living weed plants, and five days after inoculation, the weed plants became withered and necrotic. Seven days after inoculation, the fresh weight reductions of G. aparine var. tenerum, C. album, M. crispa, P. lapathifolium and A. fatua were 87.25, 78.46, 82.25, 62.11, and 80.27%, respectively. Galium aparine var. tenerum and M. crispa exhibited significant reductions in fresh weight. The bio-safety test on the five crops showed no significant plant height reductions, which was also observed for wheat(Triticum aestivum), faba bean(Vicia faba), and barley(Hordeum vulgare). By contrast, oilseed rape(Brassica napus) and pea(Pisum sativum) exhibited light spots but no significant reductions in plant height. These results indicated that A. pullulans could be a potential microbial herbicide for the control of the target weeds in crops. Optimization of the carbon and nitrogen sources for cultural media and substances for solid-state fermentation indicated that PA-2 had better colony growth and spore production with the optimal carbon source of glucose(C4), nitrogen source of soybean flour(N2), and the optimal substance was wheat bran. The results in this study provide useful information for the development ofA. pulluans PA-2 as an herbicide for bio-control of the weed.