Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

High-quality Development of the Pearl River Night Tour in the Context of Deep Integration of Culture and Tourism

Urban night tourism is a new form of urban tourism development and an important driving force for the prosperity of urban night economy.As the most prestigious business card among many night tour projects in Guangzhou,the Pearl River water night tour has contributed to the development of Guangzhou s night economy,which cannot be underestimated.However,in the process of rapid development,there are also problems such as diversified operators,insufficient integration of intangible cultural elements,excessive commercial attributes,issues of tourism facilities and service facilities.Under the new stage of integrated development of culture and tourism,how to use mutual promotion of culture and tourism in the Pearl River night tour to seek innovative paths for high-quality development in the future is the main problem expected to be solved in this paper.Therefore,based on the perspective of culture and tourism integration,this paper analyzes the general situation and development status of the Pearl River night tour,and proposes five development strategies:the overall planning of culture and tourism integration,the deep integration of intangible cultural elements,the balance of commercial attributes and tourism attributes,the high integration of culture,business,tourism and education,and the creation of tourism atmosphere,in order to provide inspiration for the healthy,orderly and high-quality development of the Pearl River night tour.

Development Strategies for Z County E-sports Town in Chongqing:Integration of E-sports,Tourism,and Agriculture under New Policies

Chongqing Z County E-sports Town was once included in the“first batch of sports and leisure characteristic town pilot project list”issued by the General Administration of Sports of China.After several years of development,the e-sports industry in Z County e-sports town has struggled with issues such as a limited market,low popularity,poor business performance,and a lack of income generation capabilities.These challenges have severely hindered the town’s development,resulting in its exclusion from the recent“Chongqing characteristic town list.”Under the requirements of new policies,it can no longer promote itself,attract investment,or undertake project construction under the name of a“characteristic town”and must undergo redevelopment.This paper employs case analysis and comparative investigation methods to conduct an in-depth empirical study on the development of Z County E-sports Town.It is proposed that under the new policy environment,the town should foster the deep integration of its existing e-sports industry and culture with tourism and agriculture,transforming Z County E-sports Town into a new characteristic tourist destination.The findings of this paper offer a reference for the development of similar towns facing stagnation and provide case support for the construction of tourist destinations featuring e-sports.

Research on Health Tourism Development Based on Taoist Culture——A Case Study of Mao Mountain Scenic Area in Jurong City of China

In accordance with relevant theories of health tourism and Taoist health culture,backgrounds for health tourism development in Mao Mountain had been analyzed from perspectives of geographic conditions,environmental resources and source market.It pointed out that brand effect of Mao Mountain Scenic Area had initially formed and "snack" tourism became prominent.After conclusion of necessity and feasibility to develop health tourism in Mao Mountain,it proposed strategies to develop health tourism in Mao Mountain which were "indentifying Taoist health theme,creating harmonious ecological environment,developing characteristic products and completing service system".

Development Strategies of Tea Culture Tourism in the Age of Experience Economy

Experience economy is the fourth development phase following the agrarian economy,the industrial economy and the service economy.In terms of tourist economy,travelling itself is a kind of experience activity,thus such an attribute determines that tourist products should satisfy the individualized needs of tourists.This study proposes that the theory of experience economy can be applied in the development of tea culture tourism to explore the functions of tea culture which could be experienced by tourists,such as leisure experience,health experience,culture experience and so on,and meanwhile,gives strategies of developing tea culture tourism in the age of experience economy.

Study on Tourism Development of the Remains of Dawenkou Culture Based on SWOT Analysis

SWOT Analysis is applied in this study to analyze strengths,weaknesses,opportunities and threats for the tourism development of Dawenkou Culture,which will help Dawenkou area to meet the challenges,seize the opportunities,and adopt pertinent measures for the scientific planning of local tourist resources.The authors suggest promoting the propaganda,improving infrastructures and creating a favorable atmosphere for tourism development,and moreover,study the strategy selection in tourism development of Dawenkou Culture.

Dalu VillageA Probe into Preservation and Sustainable Development of Chinese Couplet Culture in Dalu Village

Dalu Village of Guangxi province is one of the national historical and cultural villages in China.It enjoys great fame for its Chinese couplets which are viewed as a distinctive cultural heritage passed down by its ancestors roughly 400 years ago.The couplets are a cultural art of great value worth preserving and inheriting.However,with urbanization and tourist development,the couplets are under the threat of devastation and disappearance.Based on desk research and field research,studies on the value of the couplets in Dalu Village and analysis of the rising crises are presented in the paper.In addition,solutions consisting of restoration,repair and ecomuseum are put forward as an attempt to avert the crises and promote the sustainable development of Dalu Village’s couplet culture in the long run.

Discussion about Tourism Development of Agricultural Cultural Heritage in Xiajin County of Shandong Province

[Objectives]To discuss the protection and utilization of agricultural cultural heritage in Xiajin County in the context of deep integration of culture and tourism.With the advancement of the process of revitalizing villages through culture and tourism,agricultural cultural heritage has gradually attracted wide attention from all walks of life.This paper is intended to provide new ideas and strategies for the development of local cultural tourism through in-depth analysis of agricultural cultural heritage resources in Xiajin County.[Methods]Using the method of literature research,this paper systematically sorted out the historical background,current situation and value of agricultural cultural heritage in Xiajin County.Besides,the agricultural cultural heritage resources in Xiajin County were comprehensively evaluated using the 3-3-6 evaluation method.On this basis,combined with the theory of industrial convergence and sustainable development,it came up with some specific suggestions on the tourism development of agricultural cultural heritage in Xiajin County.[Results]Through the in-depth evaluation of agricultural cultural heritage resources in Xiajin County,it is found that it has rich historical and cultural heritage and unique tourism value.However,there are still some problems in protection and utilization.In view of the problems,it recommended to attach importance to the inheritance of Xiajin agricultural cultural heritage,develop characteristic heritage tourism and improve relevant supporting facilities.[Conclusions]Deeply excavating the essence of agricultural cultural heritage in Xiajin County and organically combining it with modern tourism is an important way to realize the sustainable development of local cultural tourism.The recommendations are expected to provide reference for the development of agricultural heritage tourism in Xiajin County and other regions,and help to promote the deep integration of culture and tourism,and promote rural revitalization and cultural inheritance.

Integrated Development of Tea and Tourism in Taishan Mountain Tea Valley in the Context of Rural Revitalization

In recent years,the integrated development of the tea and tourism has become an important way to promote the rural revitalization of Tai'an tea-producing areas,but the state of industrial upgrading still needs to be improved.In order to better integrate tea and tourism in Tea Valley,this paper uses questionnaire and SWOT analysis to study.It found the following problems:(i)45.7%of tourists were interested in tea culture,but they know litle about it,and the integration of tea culture and tea tourism products is low.(i)50.54%of the tourists were interested in the tea picking experience project and wanted to add the tea food experience item.(ii)The acceptance of 45.7%of tourists to scenic spots was 1-2 h,which shows that the quality of transportation service facilities and personnel needs to be improved.Combined with the SWOT analysis,it is found that the scenic spot is rich in resources,convenient transportation,profound cultural heritage,and good govern-ment policy support conditions,which is suitable for the SO strategy(growth development strategy),so the scenic spot relies on internal ad-vantages and seizes external opportunities to develop the integration of tea and tourism.Based on the analysis results,it came up with the perti-nent recommendations for the key problems:(i)The infrastructure service is not well established.It is suggested that scenic spots should in-crease investment,regularly train service personnel to improve their quality,and improve transportation services,accommodation,catering and public service hardware facilities.(i)Tea tourism products are insufficient.It is suggested that tea tourism products should be innovatively designed and tea tourism experiential products and tourism commodities should be planned.(i)Tea culture and tourism lack organic combi-nation.It is recommended to deeply excavate tea culture,and take consideration of both spiritual and material aspects.The specific methods include building teahouses,Taishan Mountain(Mount Tai)tea culture throughout the whole process of tea production,development and pro-cessing,and AR tea culture experience hall can be built to make tourists feel tea culture from dfferent perspectives.(iv)The publicity is in-sufficient.Strategies should be made in three aspects:improving the Internet media,taking into account the traditional media,and expanding the market based on the surrounding areas.(v)The tea industry chain is short.It is recommended to add four new industrial chain designs of"tea+accommodation,tea+catering,tea+Intemet,tea+training",hoping to bring some inspiration and ideas to the managers of scenic spots.

Development Strategy of Sichuan Sports Museum under the Background of Cultural and Tourism Integration

Through conducting a SWOT analysis of the Chengdu Sports Institute Museum,and utilizing the 4M management,5W display,and 4P marketing theoretical models,this study explores effective strategies suitable for Sichuan sports museums in terms of functionality,display,and marketing.

Construction Method of Civil Aircraft Airborne⁃system Supplier Development Process System

Based on the analysis of the development process system status of domestic and foreign civil aircraft airborne system suppliers,this paper proposes the overall construction idea of"a set of civil aircraft process system"based on IPD(Integrated product development)in the organization.The development stage,process,activity and task are the logical framework elements of the system construction."Based on process decomposition,vertical stratification,horizontal segmentation,combination of special and common,process customized to tools,role and process integration,giving full consideration to the interface between supplier management and process modules"etc.are the concrete ideas.Moreover,formulate top⁃level system construction standards,implemented by process system management tool platform,so that the process system and development work can be effectively integrated to effectively guide the development work of airborne system suppliers,and meet the quality and airworthiness requirements of civil aircraft development.Through the development application of a certain type of flight control system,the process system and tool platform were verified and optimized in practice.

Research on the Enhancement of Chongqing Cyberpunk Cultural Tourism Experience under the Background of Culture and Tourism Integration

As a new trend in tourism and cultural development,the integration of culture and tourism is capturing the zeitgeist with its unique charm.In this context,various regions have explored distinctive cultural tourism resources and created unique tourism brands through in-depth exploration and innovation of local culture.Chongqing,with its unique geomorphological features and distinctive architectural style,retains the deep flavor of traditional Chinese culture while cleverly integrating the futuristic sense of modern technology.This combination aligns perfectly with today’s popular Chinese cyberpunk style,making Chongqing an ideal place for the development of cyberpunk cultural tourism.This article investigates the history of cyberpunk culture and its current development status in Chongqing through a literature review.It then discusses the feasibility of developing cyberpunk cultural tourism in terms of social and cultural development needs and market demand.Based on a survey of cyberpunk tourists’experiences,the article deeply analyzes the existing problems of cyberpunk cultural tourism in Chongqing and proposes corresponding experience enhancement strategies,aiming to provide references for the development of cyberpunk cultural tourism in Chongqing Municipality.

Research on the Path to High-Quality Development of Grassroots Libraries in the Era of Artificial Intelligence in Henan

Since the age of digital intelligence,the government has introduced policies to actively promote the intelligent transformation of grassroots public cultural services.Based on the investigation and analysis of the development status quo of grassroots libraries in Henan Province,we put forward the path of high-quality development of grassroots libraries in Henan,namely,improving the mechanism and system,leading with digital intelligence technology,empowering by Yellow River culture(the heritage and values rooted in the history and traditions of the Yellow River region),and driven by users’demand.

A Study on the Integration of Chinese Culture in High School English Teaching

The inseparable relationship between language and culture indicates that culture teaching is indispensable in English teaching.The ultimate goal of culture teaching in English teaching is to cultivate learners’cross-cultural communication skills.Cross-cultural communication is a two-way process,which requires not only knowledge of English culture but also the use of English as a medium for disseminating Chinese culture to the outside world.However,in actual English teaching,the teaching of Chinese culture is a mere formality,and the teaching effect is not obvious.Therefore,it is necessary to conduct a survey and research on Chinese culture teaching in high school English classrooms to understand its current situation,existing problems,and their causes,so as to provide suggestions for Chinese culture teaching in high school.

Development of Sustainable Urban-Rural Integration:Dongtan Case Study

The environmental impact issues,such as global warming and expansion of the urban zone,seem more serious and have become the biggest defining challenges of the 21st century.Climate change can lead to water shortages,desertification,land degradation,air pollution,rising sea levels,accelerated deforestation,and exacerbated economic pressures.Global urban growth greatly impacts changes in sociability,humanity,and the environment of the Earth.The human presence,especially in cities,seriously affects resource use and waste disposal,and they are consuming natural resources faster than the planet can sustain during urbanization,changing how people live.China,with a population of 1.3 billion,has seen tens of millions of people living in the countryside migrate to cities,especially megacities,since the 1980s.As a result of its decision to industrialize and urbanize to boost the economy,China has become the world’s second-largest consumer of energy.In recent years,China’s government has quickly recognized the lessons of“limits to growth”and has taken action by initiating the construction process in Dongtan,Shanghai,China.They are making efforts to build urban-rural integration communities to promote sustainable development.Based on a literature review focusing on Dongtan,research questions are raised according to the research objective:(1)What are the challenges of sustainable development in urban-rural integration?(2)What practices has Dongtan implemented for sustainable development,or how is sustainable development being applied to Dongtan?(3)What are the social,political,environmental,and economic concerns regarding the sustainable development of Dongtan?The sustainable urban-rural integration concerns the ecological,economic,environmental,and psychological aspects of urban-rural integration design and management.The overall objective is to promote sustainable development in economic,social,ecological,and spatial dimensions.It will be a liveable,complete community that makes economic,environmental,and social sense locally while also contributing to national and global sustainable development.It will serve as a compelling model for how to build sustainable urban-rural integration worldwide.

An Exploration of Organizational Development in the Process of Innovation and Transformation of Pharmaceutical Enterprises-Taking X Pharmaceutical Enterprise as an Example

Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the wave of pharmaceutical industry reform,some entrepreneurs realize the importance of innovation and technology in the future.However,domestic pharmaceutical enterprises are still in the production-oriented stage.In the process of transformative innovation,pharmaceutical enterprises should not only rely on the support of scientific and technological and economic forces,but also need a suitable organizational change and many excellent talents as the source of development.Methods Through retrieving relevant literature and combined with the organizational development practice of X pharmaceutical enterprise,the organizational development model was explored in this paper.Results and Conclusion By forming a system of strategy,organization,talent,culture and mechanism,and building an organizational development model,we can improve the organizational atmosphere and organizational efficiency in the process of enterprise innovation and transformation,so as to enhance the competitiveness of enterprises in the market.

Targeted Research on the Integration of Ideological and Political Education and Chinese Excellent Traditional Culture in Universities in Henan Province

This study aims to explore the integration of excellent traditional Chinese culture with ideological and political education in universities in Henan Province,focusing on the unique challenges and opportunities presented by the local context.Using a combination of quantitative and qualitative methods,the study analyzes the impact of cultural elements on political ideology and education in the region.The study contributes to a broader discussion of educational reform in China and provides insights for policymakers and educators seeking to develop well-rounded,culturally informed citizens.

Research on the Communication and Development of Heilongjiang Cultural and Creative Industry under the Background of“Belt and Road”

This paper focuses on the research on the dissemination and development of cultural and creative industries in Heilongjiang Province under the background of“Belt and Road.”Firstly,the paper analyzes the main characteristics of cultural and creative industries in Heilongjiang Province and then puts forward the leading advantages of the government to promote the development of cultural and creative industries.The government gives full play to its regional advantages and creates Longjiang cultural products,while also accelerating regional industrial cooperation and innovative cultural communication forms.In-depth discussion is made on integrating scientific and technological means of the times and strengthening the cultivation of innovative talents.The government optimizes the communication and development path of the Heilongjiang cultural entrepreneurship industry and brings positive influence to the stable development of the Heilongjiang region in the future.

Assessment of AMBR^TMas a model for high-throughput cell culture process development strategy

The development and delivery of high quality therapeutic products necessitates the need for highthrough-put (HTP) process development tools. Traditionally, these works requires a combination of shake flask and small-scale stirred tank bioreactor (STR) which are labor and resource intensive and time-consuming. Here we demonstrate a strategy for rapid and robust cell culture process development by evaluating and implementing the use of a new HTP disposable micro bioreactor (MBR) called AMBRTM system (Advanced Microscale Bioreactor) that has the capabilities for automated sampling, feed addition, pH, dissolved oxygen (DO), gassing and agitation controls. In these studies the performance of two monoclonal antibody (MAb) producing cell lines (MAb1 and MAb2) was evaluated both in the AMBR system and 3-L STR. We demonstrated that cell culture performance (growth and viability, production titer and product quality) were similar in both vessel systems. Furthermore, process control and feed optimization were demonstrated in an additional cell line (MAb3) in the disposable MBR and its performance confirmed at STR scale. The results indicate that the AMBR system can be used to streamline the process development effort and facilitate a rapid and robust cell culture process development effort for MAb programs in a HTP manner.

A Multi-Level Evaluation of Policy Integration of Human Resource Development in Agriculture Sector

Human resource development assumes as a golden key for a knowledge-based economy and competitiveness. This study evaluates policy integration of human resource development (HRD) in agricultural sector within the context of multi-level governance (i.e., interdependence of administrative levels in decision making) and suggests means to enhance policy integration in Bangladesh. Conducting a critical review of major policy instruments, key informant interview and two case studies, this study concluded that HRD issue has fairly included and observed consistency (between the aim of HRD and policy) in all policies and strategies;majority of policy instruments have significantly addressed the significance of HRD and added plans for its development;reporting, monitoring and evaluation of policies and strategies are rather weak and there is no adequate flow of financial arrangement that is largely determined by the availability of government project. Based on the findings, the following means should be useful for improving HRD policy integration such as providing a handsome amount of development budget at the sub-district agricultural office, formulating coherent strategies to build effective cooperation, coordination and participation among stakeholders, initiating special capacity building programmes for rural women, young farmers and smallholders and mainstreaming HRD issue in governmental planning and programmes, and accordingly crafting policies and implementation.