“Essentials of Biotechnology”来华留学英语授课品牌课程建设纪实

经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原有双语课程教学的基础上,通过优化教材和教学内容,打造双向教学模式和建设多元评价体系并持续改进,构建了多层次启发式、互动、研讨相结合的复合教学体系,显著增强留学生专业基础知识水平和自主学习能力,为后续课程学习和研究工作的开展奠定了良好的基础。教学实践结果表明,该教学体系对提高该类课程的学习成效和课程质量,具有较为显著的示范效应。

HP Storage Essentials

HP Storage Essentials解决方案服务可帮助客户安装、集成和优化其存储区域管理解决方案的效率和性能，面向服务器和存储网络的统一管理平台将帮助企业大大缩短解决问题的时间，更加有效地进行故障排除和事件关联，同时降低总体管理成本。

Potential Use of Essentials Oils to Control Fruitlet Core Rot (FCR) in Pineapple (Queen Victoria Variety) in Reunion Island

Fruitlet Core Rot (FCR) is one of the major postharvest diseases of pineapple (Ananas comosus var. comosus), especially on the prone variety Queen Victoria cultivated in Reunion Island. This aggressive disease is generally due to two pathogens: Fusarium ananatum and Thalaromyces stolii, and needs to be controlled during postharvest. In Reunion Island, F. ananatum is the principal causal agent impacting fruit exportation. Fruit produced for the export market is generally treated with chemicals. This type of postharvest treatment is not in line with consumer expectations, as consumers prefer fruits treated with nonharmful and natural products. The objective of this work was to study alternative postharvest treatments using the fungitoxic properties of essential oils and their ability to elicit the resistance mechanisms of the fruit. Six EAs were tested in vitro on mycelial growth of F. ananatum. Whether by volatilization or included in the culture medium, some essential oils such as thymol, eugenol, geraniol and the carvone/menthol mixture have a strong fungistatic effect. But only thymol has a fungitoxic effect. The thymol, a natural molecule synthesized by thyme (Thymus vulgaris L. thymoliferum) is the more effective in vitro and is the strongest potential to be used in postharvest treatment. Thymol, prepared at 0.025% in a terpene solvent that acts as a penetrating agent, was tested in vivo with inoculated fruits. The treatment was effective only on necrosis development from the upper part of the fruits. Pineapple polyphenol biosynthesis appears to have been suppressed by thymol treatment. Results and opportunities for this treatment are discussed. Additional experiments must be carried out in order to decide on the advisability of this type of treatment.

托福®Essentials™考试正式上线

2021年5月19日,美国教育考试服务中心(ETS)举办全球线上发布会,正式宣布推出托福®Essentials™考试。托福®Essentials™考试是一项全新的英语能力测评考试,是托福®家族系列测评中仅次于15年前推出的托福iBT考试的第二个高利害英语语言能力测试。该考试将于2021年6月17日(星期四)向中国考生开放报名,首次考试将于8月21日(星期六)进行。

托福Essentials对我国中学英语考试的启示

本文从基本情况、考试内容和成绩报告三个方面对托福Essentials进行了研究。研究发现:(1)该考试较为便捷,考生在家便可进行考试,多阶段自适应技术有效减少了试题数量,缩短了考试时间;(2)试题任务有较强的真实性和交互性;(3)成绩报告重点突出,内容翔实,能有效引导考生和社会正确使用考试结果。托福Essentials涵盖了从入门级到精通级的所有能力层级,代表了语言测试计算机化的发展趋势,对我国的中学英语考试形式与内容改革具有一定的启示。

ESSENTIALS

ASUS灵耀U4100UQ商务本商务人士都希望在职场上突破自我,实现自我价值。华硕灵耀U4100UQ的超窄边框恰恰诠释了这一职场理念。灵耀U4100UQ采用7.18mm超窄边框,将14英寸规格的屏幕装入纤薄的13英寸规格机身内,视觉上给人一种突破束缚、无边无际的感觉!大屏幕带来的广阔视野,不仅能提升商务人士的办公效率,还能为用户带来更良好的观影体验。

ESSENTIALS

ASUS灵耀3轻薄型笔记本电脑作为华硕旗下轻薄型笔记本电脑的＂代言人＂,灵耀3拥有约11.9mm的纤薄机身。配置第七代Intel Coer i5-7200U处理器,8GB内存,能够提供强劲的应用处理能力。在强大的处理性能下,灵耀3可以轻松满足用户影音娱乐和日常办公的需求。另外,灵耀3还搭载速度超群的256GB SSD固态硬盘,在SSD助力下,灵耀3加载大型应用程序时可以更疾速,从而提高工作效率。

优化家庭影院系统的秘密武器——《Video Essentials》DVD测试碟简介

很多朋友在玩家庭影院时,总是羡慕别人家的效果好;不少朋友花不菲的价格买了家庭影院,搬回家后却总是感叹不如在商家的试音室里的效果好。这些情形可以说并不少见,其实,除了器材本身的素质先天不足之外,更主要的原因在于缺乏家庭影院系统的调试技巧。

HEDNA workshop on revenue management essentials for edistribution

The Hotel Electronic Distribution Network Association (HEDNA), in conjunction with their education management partner, HSA International, will host the new two-day "fundamentals" workshop on applying Revenue Management techniques within Electronic Distribution channels in advance of the opening of the HEDNA 2004 Conference in London.

Effects of Supplemental Glutamine and Lysine on Growth Performance of Broiler Chickens

The optimum levels of Lysine and Glutamine needed for growth performance and maintenance of the chicken broilers were evaluated in a randomized 3 × 4 factorial arrangement of dietary treatments. The battery cages measured 99 × 66 × 25 cm that can be sufficient for 5 birds. Day old Chicken broilers totaling 180 were assigned to dietary treatments comprising of 3 concentrations of Lysine (0.85, 1.14, and 1.42) each in combination with 4 concentrations of Glutamine (0, 1, 2, and 3). Each dietary treatment was replicated 3 times and each replication had 5 birds. The birds were given feed and water ad libitum with a 23-hour light regimen for a period of 4 weeks. Then, the experimental birds were evaluated for body weight gain, feed consumption, and feed conversion in order to determine their optimum requirement for dietary Lysine and Glutamine. Based on the findings of this study, the highest performance was observed in birds fed the diet supplemented with 1.42 lysine and 1% glutamine, but the highest improvement in feed conversion was observed in diet contain 1.14 and 1.42 with 1% and 3% glutamine, respectively. Birds fed 1.42 lysine and 1% glutamine had the highest total body weight gain and feed consumption. The lysine requirements in the diet for Chicken are between 1.14 and 1.42 with glutamine level of 1%.

Key Points and Difficulties in the Management of a New Guangzhou 12th People’s Hospital Branch

During the 13th Five-Year Plan period,to improve medical and health services,the Guangzhou Municipal government invested billions in medical construction.During that time,Guangzhou was renewing its approval procedures for construction projects,and the COVID-19 outbreak changed the requirements of hospital construction.In addition to focusing more on spatial planning and layout,the integration of specialized departments,large-scale medical equipment,and numerous intelligent systems increased the complexity of hospital construction projects.The success of these projects often hinges on the quality of the management team.If a hospital construction project manager lacks experience and an understanding of the project’s key aspects,they may struggle with organization,management,and problem-solving,leading to rework,resource waste,and delays.This paper uses the construction of a new Guangzhou 12th People’s Hospital branch as an example to describe the key points and difficulties in each stage of project construction and proposes solutions for the management of similar projects in the future.

Dietary supplementation of benzoic acid and essential oils combination enhances intestinal resilience against LPS stimulation in weaned piglets

Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been fully elucidated.Previous research has primarily focused on the reparative effects of BAO on intestinal injury,while neglecting its potential in enhancing intestinal stress resistance.Methods In this study,we investigated the pre-protective effect of BAO against LPS-induced stress using a modified experimental procedure.Piglets were pre-supplemented with BAO for 14 d,followed by a challenge with LPS or saline to collect blood and intestinal samples.Results Our findings demonstrated that BAO supplementation led to significant improvements in piglets’final weight,average daily gain,and feed intake/body gain ratio.Additionally,BAO supplementation positively influenced the composition of intestinal microbiota,increasing beneficial Actinobacteriota and Alloprevotella while reducing harmful Desulfobacterota,Prevotella and Oscillospira.Furthermore,BAO supplementation effectively mitigated oxidative disturbances and inflammatory responses induced by acute LPS challenge.This was evidenced by elevated levels of T-AOC,SOD,and GSH,as well as decreased levels of MDA,TNF-α,and IL-6 in the plasma.Moreover,piglets subjected to LPS challenge and pre-supplemented with BAO exhibited significant improvements in intestinal morphological structure and enhanced integrity,as indicated by restored expression levels of Occludin and Claudin-1 compared to the non-supplemented counterparts.Further analysis revealed that BAO supplementation enhanced the jejunal antioxidative capacity by increasing GSH-Px levels and decreasing MDA levels under the LPS challenge and stimulated the activation of the Nrf2 signaling pathway.Additionally,the reduction of TLR4/NF-κB/MAPK signaling pathways activation and proinflammatory factor were also observed in the jejunal of those piglets fed with BAO.Conclusions In summary,our study demonstrates that pre-supplementation of BAO enhances the anti-stress capacity of weaned piglets by improving intestinal microbiota composition,reinforcing the intestinal barrier,and enhancing antioxidative and anti-inflammatory capabilities.These effects are closely associated with the activation of Nrf2 and TLR4/NF-κB/MAPK signaling pathways.

Causal associations between gastroesophageal reflux disease and essential hypertension: A bidirectional Mendelian randomization study

BACKGROUND Clinical studies have reported that patients with gastroesophageal reflux disease(GERD)have a higher prevalence of hypertension.AIM To performed a bidirectional Mendelian randomization(MR)analysis to investi-gate the causal link between GERD and essential hypertension.METHODS Eligible single nucleotide polymorphisms(SNPs)were selected,and weighted median,inverse variance weighted(IVW)as well as MR egger(MR-Egger)re-gression were used to examine the potential causal association between GERD and hypertension.The MR-Pleiotropy RESidual Sum and Outlier analysis was used to detect and attempt to reduce horizontal pleiotropy by removing outliers SNPs.The MR-Egger intercept test,Cochran’s Q test and“leave-one-out”sen-sitivity analysis were performed to evaluate the horizontal pleiotropy,heterogen-eities,and stability of single instrumental variable.RESULTS IVW analysis exhibited an increased risk of hypertension(OR=1.46,95%CI:1.33-1.59,P=2.14E-16)in GERD patients.And the same result was obtained in replication practice(OR=1.002,95%CI:1.0008-1.003,P=0.000498).Meanwhile,the IVW analysis showed an increased risk of systolic blood pressure(β=0.78,95%CI:0.11-1.44,P=0.021)and hypertensive heart disease(OR=1.68,95%CI:1.36-2.08,P=0.0000016)in GERD patients.Moreover,we found an decreased risk of Barrett's esophagus(OR=0.91,95%CI:0.83-0.99,P=0.043)in essential hypertension patients.CONCLUSION We found that GERD would increase the risk of essential hypertension,which provided a novel prevent and therapeutic perspectives of essential hypertension.

Recent advances in essential oils and their nanoformulations for poultry feed

Antibiotics in poultry feed to boost growth performance are becoming increasingly contentious due to concerns over antimicrobial resistance development.Essential oils(EOs),as natural,plant-derived compounds,have demonstrated antimicrobial and antioxidant properties.EOs may potentially improve poultry health and growth performance when included in poultry feed.Nevertheless,the incorporation of EOs as nutritional additives is hindered by their high volatility,low water solubility,poor intestinal absorption,and sensitivity to environmental conditions.Recently,nanoencapsulation strategies using nanoformulations have emerged as a potential solution to these challenges,improving the stability and bioavailability of EOs,and enabling targeted delivery in poultry feed.This review provides an overview of the antioxidant and antibacterial properties of EOs,the current limitations of their applications in poultry feed,and the recent advancements in nano-engineering to overcome these limitations.Furthermore,we outline the potential future research direction on EO nanoformulations,emphasizing their promising role in advancing sustainable poultry nutrition.

Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR

Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused by Xanthomonas. In this study, Xanthomonas oryzae pv. oryzae(Xoo), which causes rice bacterial leaf blight, has been studied as a representative. A transposon insertion library of Xoo, comprising approximately 200,000 individual insertion mutants, was generated. Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7–36.4% of all potential insertion sites, revealing 491 essential genes required for the growth of Xoo in rich media. The results show that, compared to the functions of essential genes of other bacteria, the functions of some essential genes of Xoo are unknown, 25 genes might be dangerous for the Xanthomonas group, and 3 are specific to Xanthomonas. High-priority candidates for developing broad-spectrum, Xanthomonas-specific, and environment-friendly bactericides were identified in this study. In addition, this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR) in combination with high performance liquid chromatography–mass spectrometry(HPLC–MS). The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action. In conclusion, this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight, and disclosed selected targets of a novel bactericide.

Oxygen is an essential gasotransmitter directly sensed via protein gasoreceptors

The current restrictive criteria for gasotransmitters exclude oxygen(O_(2))as a gasotransmitter in vertebrates.In this manuscript,I propose a revision of gasotransmitter criteria to include O_(2) per se as a signaling molecule and'essential gasotransmitter'for vertebrates.This revision would enable us to search for protein-based O_(2)-binding sensors(gasoreceptors)in all cells in the brain or other tissues rather than specialized tissues such as the carotid body or gills.If microorganisms have protein-based O_(2)-binding sensors or gasoreceptors such as DosP or FixL or FNR with diverse signaling domains,then eukaryotic cells must also have O_(2)-binding sensors or gasoreceptors.Just as there are proteinbased receptor(s)for nitric oxide(GUCY1A,GUCY1B,CLOCK,NR1D2)in cells of diverse tissues,it is reasonable to consider that there are protein-based receptors for O_(2) in cells of diverse tissues as well.In mammals,O_(2) must be acting as a gasotransmitter or gaseous signaling molecule via protein-based gasoreceptors such as androglobin that very likely mediate acute sensing of O_(2).Accepting O_(2) as an essential gasotransmitter will enable us to search for gasoreceptors not only for O_(2) but also for other nonessential gasotransmitters such as hydrogen sulfide,ammonia,methane,and ethylene.It will also allow us to investigate the role of environment-derived metal ions in acute gas(or solute)sensing within and between organisms.Finally,accepting O_(2) per se as a signaling molecule acting via gasoreceptors will open up the field of gasocrinology.

A verifiable essential secret image sharing scheme based on HLRs(VESIS-(t,s,k,n))

In traditional secret image sharing schemes,a secret image is shared among shareholders who have the same position.But if the shareholders have two different positions,essential and non‐essential,it is necessary to use essential secret image sharing schemes.In this article,a verifiable essential secret image sharing scheme based on HLRs is proposed.Shareholder's share consists of two parts.The first part is produced by the shareholders,which prevents the fraud of dealers.The second part is a shadow image that is produced by using HLRs and the first part of share.The verification of the first part of the shares is done for the first time by using multilinear and bilinear maps.Also,for verifying shadow images,Bloom Filters are used for the first time.The proposed scheme is more efficient than similar schemes,and for the first part of the shares,has formal security.

Magnetic resonance-guided focused ultrasound for essential tremor:a prospective,single center,single-arm study

The safety and effectiveness of magnetic resonance-guided focused ultrasound thalamotomy has been broadly established and validated for the treatment of essential tremor.In 2018,the first magnetic resonance-guided focused ultrasound system in Chinese mainland was installed at the First Medical Center of the PLA General Hospital.This prospective,single center,open-label,single-arm study was part of a worldwide prospective multicenter clinical trial(ClinicalTrials.gov Identifier:NCT03253991)conducted to confirm the safety and efficacy of magnetic resonance-guided focused ultrasound for treating essential tremor in the local population.From 2019 to 2020,10 patients with medication refractory essential tremor were recruited into this open-label,single arm study.The treatment efficacy was determined using the Clinical Rating Scale for Tremor.Safety was evaluated according to the incidence and severity of adverse events.All of the subjects underwent a unilateral thalamotomy targeting the ventral intermediate nucleus.At the baseline assessment,the estimated marginal mean of the Clinical Rating Scale for Tremor total score was 58.3±3.6,and this improved after treatment to 23.1±6.4 at a 12-month follow-up assessment.A total of 50 adverse events were recorded,and 2 were defined as serious.The most common intraoperative adverse events were nausea and headache.The most frequent postoperative adverse events were paresthesia and equilibrium disorder.Most of the adverse events were mild and usually disappeared within a few days.Our findings suggest that magnetic resonance-guided focused ultrasound for the treatment of essential tremor is effective,with a good safety profile,for patients in Chinese mainland.

Essential proteins identification method based on four-order distances and subcellular localization information

Essential proteins are inseparable in cell growth and survival. The study of essential proteins is important for understanding cellular functions and biological mechanisms. Therefore, various computable methods have been proposed to identify essential proteins. Unfortunately, most methods based on network topology only consider the interactions between a protein and its neighboring proteins, and not the interactions with its higher-order distance proteins. In this paper, we propose the DSEP algorithm in which we integrated network topology properties and subcellular localization information in protein–protein interaction(PPI) networks based on four-order distances, and then used random walks to identify the essential proteins. We also propose a method to calculate the finite-order distance of the network, which can greatly reduce the time complexity of our algorithm. We conducted a comprehensive comparison of the DSEP algorithm with 11 existing classical algorithms to identify essential proteins with multiple evaluation methods. The results show that DSEP is superior to these 11 methods.

Profiles of the Headspace Volatile Organic and Essential Oil Compounds from the Tunisian Cardaria draba (L.) Desv. and Its Leaf and Stem Epidermal Micromorphology

In this work, we investigated aroma volatiles emanated by dry roots, stems, leaves, flowers, and fruits of Cardariadraba (L.) Desv. growing wild in Tunisia and its aerial part essential oils (EOs) composition. A total of 37 volatileorganic compounds (96.7%–98.9%) were identified;4 esters, 4 alcohols, 7 hydrocarbons, 12 aldehydes, 5 ketones,1 lactone, 1 organosulfur compound, 2 organonitrogen compounds, and 1 acid. The hydrocarbons form the maingroup, representing 49.5%–84.6% of the total detected volatiles. The main constituent was 2,2,4,6,6-pentamethylheptane(44.5%–76.2%) reaching the highest relative percentages. Forty-two compounds were determined in thetwo fractions of EOs, representing 98.8% and 97.2% of the total oil composition, respectively. The principal componentswere hexadecanoic acid (34.6%), 6-methyl-5-hepten-2-one (18.3%), decanal (15.0%), 6,10,14-trimethyl-2-pentadecanone (13.2%), and n-pentacosane (13%). Micromorphological details of the leaf and stem epidermisusing light microscopy revealed polygonal cells with sinuous walls in the adaxial and abaxial leaf surfaces andnearly rectangular and long ones with linear and thick walls for the stem epidermis. The stomata complexes wereanisocytic in the leaf epidermis and mainly anisocytic and rarely paracytic in the stem epidermis. Non-glandulartrichomes were unbranched and long with an acute apex or short with a convex apex. The glandular ones wereidentified for the first time in this species. They were short-stalked with a large secretory head. The highest stomatalindex (17.02%) was recorded in the abaxial leaf surface. The identification of headspace volatiles and essentialoil compounds can be used to characterize this species, and the various epidermis micromorphologicalfeatures are very useful for biosystematics taxonomic studies within Brassicaceae.