The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In h...The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.展开更多
BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are...BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.展开更多
Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the ...Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the root system of wheat under dryland areas in southwestern China. The aim of this study was to evaluate the effect of three tilage treatments (no-til, NT; rotary til, RT; conventional til, CT) and two crop residue management practices (straw mulch, ML; non-straw mulch, NML) on stand establishment, root growth and grain yield of wheat. NT resulted in lower soil cover thickness for the wheat seed, higher number of uncovered seeds, lower percentage of seedling-less ridges and lower tiler density compared to RT and CT; ML resulted in higher tiler density compared to NML. Straw mulching resulted in more soil water content and root length density (RLD) at most of the growth stages and soil depths. The maximum RLD, root surface area density and root dry matter density were obtained under NT. In the topmost 10 cm soil layer, higher RLD values were found under NT than those under RT and CT. There were no signiifcant differences in the yield or yield components of wheat among the tilage treatments in 2011–2012, but NT resulted in a signiifcant higher yield compared to RT and CT in 2012–2013. Grain yield was signiifcantly higher in ML compared to in NML. A strong relationship was observed between the water-use efifciency and the grain yield. Both NT and ML proved beneifcial for wheat in term of maintaining higher tiler density, better soil water status and root growth, leading to a higher grain yield and enhanced water-use efifciency, especialy in a low rainfal year.展开更多
Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of t...Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of tillage(conventional and no-tillage system) and crop establishment methods(transplanting at a spacing of 20 cm × 20 cm with one seedling per hill and direct seeding at a seeding rate of 22.5 kg ha-1). Our results revealed that, at maximum tillering(Max.) and at maturity(MA) stages, direct seeding(DS) resulted in 22% more tillers than transplanting(TP) irrespective of tillage system. Tiller mortality reached a peak between panicle initiation(PI) and booting(BT) stages, and was 16% higher under conventional tillage(CT) than under no-tillage(NT). Transplanting required 29% more time for the completion of tillering and less for DS. Tillering rate was 43% higher in DS than TP under either CT or NT. There was a positive correlation between panicle number per m2and maximum tiller number per m2, but not panicle-bearing tiller rate. The panicle bearing tiller rate was higher under DS than TP and higher under NT than CT. Tiller dry weight gradually increased up to heading(HD) stage, and was 14% higher under TP than DS. Leaf area(cm2tiller-1) gradually increased from Max. to HD stage and then decreased by 34% in conventional tillage transplanting(CTTP) and 45% in no-tillage transplanting(NTTP) from 12DAH–24DAH(days after heading), but was similar(35%) under DS under either CT or NT. Grain yield was higher under CTTP owing to the larger sink size(heavier panicle, more spikelets in per cm length of panicle) than under DS.展开更多
We established a diagnostic model to predict acute Mycoplasma pneumoniae (M. pneumonia) infection in elderly Community-acquired pneumonia (CAP) patients. We divided 456 patients into acute and non-acute M. pneumon...We established a diagnostic model to predict acute Mycoplasma pneumoniae (M. pneumonia) infection in elderly Community-acquired pneumonia (CAP) patients. We divided 456 patients into acute and non-acute M. pneumoniae infection groups. Binary logistic regression and receiver operating characteristic (ROC) curves were used to establish a predictive model. The following independent factors were identified: age 〉 70 years; serum cTNT level 〉 0.0S ng/mL; lobar consolidation; mediastinal lymphadenopathy; and antibody titer in the acute phase 〉 1:40. The area under the ROC curve of the model was 0.923 and a score of 2 7 score predicted acute M. pneumoniae infection in elderly patients with CAP. The predictive model developed in this study has high diagnostic accuracy for the identification of elderly acute M. pneumoniae infection.展开更多
Elongations of coleoptile and mesocotyl are related directly to rice seedling establishment in soil and height of plant is related to lodging in rice production. Twelve typical rice cultivars with different lengths of...Elongations of coleoptile and mesocotyl are related directly to rice seedling establishment in soil and height of plant is related to lodging in rice production. Twelve typical rice cultivars with different lengths of coleoptile and mesocotyl (long, medium and short) were selected by screening the lengths of coleoptile and mesocotyl in 1500 accessions. The seedling establishments of these typical cultivars were compared under the combinations of different sowing depths and flooding durations, and two semi-dwarf varieties (G140, Zhong 96-21) with good seedling establishments and optimum mesocotyl lengths were found. The length of mesocotyl was completely fitted negative binomial distribution and the length of coleoptile was nearly fitted Iognormal distribution. Analysis of the relationships among mesocotyl, coleoptile, seeding depth, flooding duration, and their interactions to seedling establishment percentage showed that there existed significant relations among mesocotyl, coleoptile, mesocotyl x coleoptile, seeding depth, flooding duration and mesocotyl x sowing depth in the experiment for seedling establishment.展开更多
Environmental changes are expected to shift the distribution and abundance of vegetation by determining seedling estab- lishment and success. However, most current ecosystem models only focus on the impacts of abiotic...Environmental changes are expected to shift the distribution and abundance of vegetation by determining seedling estab- lishment and success. However, most current ecosystem models only focus on the impacts of abiotic factors on biogeophysics (e.g., global distribution, etc.), ignoring their roles in the population dynamics (e.g., seedling establishment rate, mortality rate, etc.) of ecological communities. Such neglect may lead to biases in ecosystem population dynamics (such as changes in population density for woody species in forest ecosystems) and characteristics. In the present study, a new establishment scheme for introducing soil water as a function rather than a threshold was developed and validated, using version 1.0 of the IAP-DGVM as a test bed. The results showed that soil water in the establishment scheme had a remarkable influence on forest transition zones. Compared with the original scheme, the new scheme significantly improved simulations of tree population density, especially in the peripheral areas of forests and transition zones. Consequently, biases in forest fractional coverage were reduced in approximately 78.8% of the global grid cells. The global simulated areas of tree, shrub, grass and bare soil performed better, where the relative biases were reduced from 34.3% to 4.8%, from 27.6% to 13.1%, from 55.2% to 9.2%, and from 37.6% to 3.6%, respectively. Furthermore, the new scheme had more reasonable dependencies of plant functional types (PFTs) on mean annual precipitation, and described the correct dominant PFTs in the tropical rainforest peripheral areas of the Amazon and central Africa.展开更多
In Dynamic Global Vegetation Models (DGVMs), the establishment of woody vegetation refers to flowering, fertiliza- tion, seed production, germination, and the growth of tree seedlings. It determines not only the pop...In Dynamic Global Vegetation Models (DGVMs), the establishment of woody vegetation refers to flowering, fertiliza- tion, seed production, germination, and the growth of tree seedlings. It determines not only the population densities but also other important ecosystem structural variables. In current DGVMs, establishments of woody plant functional types (PFTs) are assumed to be either the same in the same grid cell, or largely stochastic. We investigated the uncertainties in the competition of establishment among coexisting woody PFTs from three aspects: the dependence of PFT establishments on vegetation states; background establishment; and relative establishment potentials of different PFTs. Sensitivity experi- ments showed that the dependence of establishment rate on the fractional coverage of a PFT favored the dominant PFT by increasing its share in establishment. While a small background establishment rate had little impact on equilibrium states of the ecosystem, it did change the timescale required for the establishment of alien species in pre-existing forest due to their disadvantage in seed competition during the early stage of invasion. Meanwhile, establishment purely fiom background (the scheme commonly used in current DGVMs) led to inconsistent behavior in response to the change in PFT specification (e.g., number of PFTs and their specification). Furthermore, the results also indicated that trade-off between irtdividual growth and reproduction/colonization has significant influences on the competition of establishment. Hence, further development of es- tablishment parameterization in DGVMs is essential in reducing the uncertainties in simulations of both ecosystem structures and successions.展开更多
Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the tr...Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the transition begins as early as in April, followed by abrupt change in May-June; the Asian summer monsoon situation is fully established in June. The winter convective center in Sumatra moved steadily northwestward across the "land bridge" of the maritime continent and the Indo-China Peninsula as time goes from winter to summer, thus giving rise to the change in large scale circulations that is responsible for the summer monsoon establishment over SE Asia and India; the South China Sea to the western Pacific summer monsoon onset bears a close relation to the active convection in the Indo China Peninsula and steady eastward retreat of the subtropical TBB high-value band,corresponding to the western Pacific subtropical high.展开更多
This study aims to establish the occupational exposure limit (OEL) in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring...This study aims to establish the occupational exposure limit (OEL) in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring. The clinical therapeutic dose was used as lowest observed effect level (LOEL), and no observed effect level (NOEL) was achieved by using a safety factor. The highest concentration of warfarin monitored in the worksite of centrifuge washing, drying and packing were 0.029 mg/m3, 0.052 mg/m3 respectively, which did not exceed the OEL 0.2 mg/m3 recommended by NIOSH and ACGIH. Considering its feasibility for enforcement and protection for workers, we recommend OEL 0.2 mg/m3 of warfarin in China.展开更多
An ideal leaf shape is beneficial to the yield of rice.Molecular understanding of the leaf primordia and polarity establishment plays a significant role in exploring the genetic regulatory network of leaf morphogenesi...An ideal leaf shape is beneficial to the yield of rice.Molecular understanding of the leaf primordia and polarity establishment plays a significant role in exploring the genetic regulatory network of leaf morphogenesis.In recent years,researchers have cloned an array of coding genes and a few non-coding small RNAs involved in rice leaf development through regulating the development of leaf primordia,vascular bundles,sclerenchyma cells,bulliform cells,cell walls and epidermis cells.These genes and their interactions play critical roles in rice leaf development through the determination and regulatory role in gene expression,and their coordination with other genetic networks or signal pathways.But the relationship among these genes is poorly defined and the underlying network is still unclear.In this review,we introduced the regulatory pathways of leaf primordium development and leaf polarity establishment,mainly the relationship between cell development mechanism and leaf polarity establishment,focusing on how leaf tissue affects leaf shape.Hopefully,the regulation network reviewed here has immediate implications for future research and genomic design breeding.展开更多
Objective:To induce pluripotent stem(IPS)cells from fibrocytes that are separated from liver cancer patients.Methods:The fibrocytes were reprogrammed to IPS cells by lentiviral vector,stained and identified by immunoh...Objective:To induce pluripotent stem(IPS)cells from fibrocytes that are separated from liver cancer patients.Methods:The fibrocytes were reprogrammed to IPS cells by lentiviral vector,stained and identified by immunohistochemistry.Results:The IPS cells were successfully established from fibrocytes after infection,and IPS cell clones formed in round shape under a microscopy.The induction rate was 0.013%±0.007%.No tumor formed at the back of nude mice within 8 weeks after the inoculation of cell clone.However,tetatoma appeared in nude mice within 1 week after IPS inoculation.A few tumors formed in nude mice within 4 weeks after the inoculation of cell clones.However,subcutaneous tumors formed within 1 week after IPS inoculation.The induced IPS cells showed three germ layers in tetatoma.Nanog and OCT4 in the induced IPS cells showed hypomethylation.SSEA-A,TRA-1-6-,TRA-1-81 and Nanog were highly expressed in the induced IPS cells,indicating the IPS cells possessed the similar ability as the stem cells.Conclusion:The IPS cells of liver cancer patients can be established effectively from fibrocytes and can be cultured stably in vitro,which provides an approach for the treatment of intermediate or advanced stage liver cancer.展开更多
Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are s...Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are still not fully understood.Animal models can be used to simulate the pathophysiological features of MRONJ,serving as standardized in vivo experimental platforms to explore the pathogenesis and therapies of MRONJ.Rodent models exhibit excellent effectiveness and high reproducibility in mimicking human MRONJ,but classical methods cannot achieve a complete replica of the pathogenesis of MRONJ.Modified rodent models have been reported with improvements for better mimicking of MRONJ onset in clinic.This review summarizes representative classical and modified rodent models of MRONJ created through various combinations of systemic drug induction and local stimulation and discusses their effectiveness and efficiency.Currently,there is a lack of a unified assessment system for MRONJ models,which hinders a standard definition of MRONJ-like lesions in rodents.Therefore,this review comprehensively summarizes assessment systems based on published peer-review articles,including new approaches in gross observation,histological assessments,radiographic assessments,and serological assessments.This review can serve as a reference for model establishment and evaluation in future preclinical studies on MRONJ.展开更多
Venereal diseases are considered to be the most prevalent infectious diseases in the worldwide. China is now faced with a year-by-year increasing incidence of sexually transmitted diseases (STD), which are spreading...Venereal diseases are considered to be the most prevalent infectious diseases in the worldwide. China is now faced with a year-by-year increasing incidence of sexually transmitted diseases (STD), which are spreading from high-risk groups to the general population. Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum and herpes simplex virus-2 (HSV-2) are always regarded as the most common venereal pathogens. The "golden standard" for testing Neisseria gonorrhoeae remains to be bacteria culture or microscopic examination.展开更多
In recent years, conventional rice production technologies have been leading to deterioration of soil health and declining farm profitability due to high inputs of water and labor. Conservation agriculture (CA) base...In recent years, conventional rice production technologies have been leading to deterioration of soil health and declining farm profitability due to high inputs of water and labor. Conservation agriculture (CA) based resource-conserving technologies i.e. zero-tillage (ZT), raised-bed planting and direct-seeded rice (DSR) have shown promise as alternatives to conventional production technologies to overcome these problems. Present study was undertaken during 2009-2012 to establish an understanding of how permanent raised bed cropping system could be practiced to save water at the field application level to improve water productivity and also have the capability to enhance productivity, profitability and soil physical quality. The results showed that among different crop establishment techniques, conventional-tilled puddle transplanted rice (CT-TPR) required 14%-25% more water than other techniques. Compared with the CT-TPR system, zero till direct-seeded rice (ZT-DSR) consumed 6%-10%less water with almost equal system productivity and demonstrated higher water productivity. Wide raised beds saved about 15%-24% water and grain yield decrease of about 8%. Direct-seeded rice after ZT or reduced tillage or on unpuddled soil provided more net income than CT-TPR. The CT-TPR system had higher bulk density and penetration resistance due to compaction caused by the repeated wet tillage in rice. The steady-state infiltration rate and soil aggregation (〉 0.25 mm) were higher under permanent beds and ZT and lower in the CT-TPR system. Under CT-TPR, soil aggregation was static across seasons, whereas it improved under no-till and permanent beds. Similarly, mean weight diameter of aggregates was higher under ZT and permanent beds and increased over time. The study reveals that to sustain the rice productivity, CA-based planting techniques can be more viable options. However, the long-term effects of these alternative technologies need to be studied under varying agro-ecologies in western Uttar Pradesh, India.展开更多
We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in...We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.展开更多
According to the principle of belt seeding establishment of adhesive bonded fabric,applied indexes of belt seeding establishment effect were studied using carrier combination of several kinds of waste meterials under...According to the principle of belt seeding establishment of adhesive bonded fabric,applied indexes of belt seeding establishment effect were studied using carrier combination of several kinds of waste meterials under soil cover.The results showed that the effect depended on the coordinated characteristics of carrier combination from turfgrass and waste materials.It was testified that production of belt seeding was feasible using a certain kind of waste material carrier under given conditions.The study provided a scientific basis for applying evvironmental engineering to belt seeding establishment of waste materials and developing nwe industry of environmental protection.展开更多
In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C18 solid phase extraction column....In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C18 solid phase extraction column. Qualitative determination was performed according to retention time and selective ion abundance ratio,and quantification was performed by external standard method. The results showed that progesterone had a good linear relation in the range of0. 1-5. 0 ng/ml,with a correlation coefficient of 0. 999 9. The detection limit of the established method was 0. 02 μg/kg,and the quantification limit was0. 04 μg/kg. In the standard addition range of 0. 1-5. 0 ng/ml,the recovery ranged from 79. 6% to 105. 1%,with relative standard deviation≤8. 9%. Compared with the industry standard,the detection limit and the quantification limit of the established method are significantly reduced,and the sensitivity of the method is enhanced,so this method is suitable for the detection of progesterone residue in fresh milk in pastures.展开更多
Field experiments with medicinal rice Njavara were conducted at Cropping Systems Research Centre, Karamana, Thiruvananthapuram, Kerala during summer of 2007 and 2008. The experimental design was split plot with three ...Field experiments with medicinal rice Njavara were conducted at Cropping Systems Research Centre, Karamana, Thiruvananthapuram, Kerala during summer of 2007 and 2008. The experimental design was split plot with three replications. The treatments consisted of four establishment techniques viz., System of Rice Intensification SRI (M1), Integrated Crop Establishment Method (ICM) (M2), Package of practices (PoP) (M3) of Kerala Agricultural University and Conventional Management Practices (CMP) (M4) in main plot. Three nutrient sources viz., (1) organic, 2) integrated use of organic and inorganic, 3) chemical fertilizers only) were used under different establishment techniques. Root dry matter production was determined at weekly intervals and plant nutrients uptake was determined by calculating from the product of dry matter, straw dry weight, grain yield and percentage of nutrients. Results revealed that at early stages (4-6 WAT/WAS) (Weeks after transplanting/Weeks after sowing), root dry matter production hill-1 was in the order of SRI > ICM > CMP > PoP which changed to SRI > ICM > PoP > CMP from 7th week onwards. Maximum root dry matter (at 9th WAS/WAT) was recorded in SRI (0.51/0.50 g.hill-1 in 2007/2008). The total nutrients uptake (N, P, K, Fe, Mn and Zn) in conventional management practices was significantly higher than other establishment techniques. Among the nutrient sources, higher total (N, P, K, Fe, Mn and Zn) uptake by crop was recorded under integrated nutrient source than organic and inorganic sources.展开更多
文摘The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration.
基金the National Natural Science Foundation of China,No.82060551and Lanzhou Chengguan District Science and Technology Planning Project,No.2019JSCX0092.
文摘BACKGROUND Extrahepatic cholangiocarcinoma sarcoma is extremely rare in clinical practice.These cells consist of both epithelial and mesenchymal cells.Patient-derived cell lines that maintain tumor characteristics are valuable tools for studying the molecular mechanisms associated with carcinosarcoma.However,cholangiocarcinoma sarcoma cell lines are not available in cell banks.AIM To establish and characterize a new extrahepatic cholangiocarcinoma sarcoma cell line,namely CBC2T-2.METHODS We conducted a short tandem repeat(STR)test to confirm the identity of the CBC2T-2 cell line.Furthermore,we assessed the migratory and invasive properties of the cells and performed clonogenicity assay to evaluate the ability of individual cells to form colonies.The tumorigenic potential of CBC2T-2 cells was tested in vivo using nonobese diabetic/severe combined immunodeficient(NOD/SCID)mice.The cells were injected subcutaneously and tumor formation was observed.In addition,immunohistochemical analysis was carried out to examine the expression of epithelial marker CK19 and mesenchymal marker vimentin in both CBC2T-2 cells and xenografts.The CBC2T-2 cell line was used to screen the potential therapeutic effects of various clinical agents in patients with cholangiocarcinoma sarcoma.Lastly,whole-exome sequencing was performed to identify genetic alterations and screen for somatic mutations in the CBC2T-2 cell line.RESULTS The STR test showed that there was no cross-contamination and the results were identical to those of the original tissue.The cells showed round or oval-shaped epithelioid cells and mesenchymal cells with spindle-shaped or elongated morphology.The cells exhibited a high proliferation ratio with a doubling time of 47.11 h.This cell line has migratory,invasive,and clonogenic abilities.The chromosomes in the CBC2T-2 cells were polyploidy,with numbers ranging from 69 to 79.The subcutaneous tumorigenic assay confirmed the in vivo tumorigenic ability of CBC2T-2 cells in NOD/SCID mice.CBC2T-2 cells and xenografts were positive for both the epithelial marker,CK19,and the mesenchymal marker,vimentin.These results suggest that CBC2T-2 cells may have both epithelial and mesenchymal characteristics.The cells were also used to screen clinical agents in patients with cholangiocarcinoma sarcoma,and a combination of paclitaxel and gemcitabine was found to be the most effective treatment option.CONCLUSION We established the first human cholangiocarcinoma sarcoma cell line,CBC2T-2,with stable biogenetic traits.This cell line,as a research model,has a high clinical value and would facilitate the understanding of the pathogenesis of cholangiocarcinoma sarcoma.
基金supported by the China Agriculture Research System(CARS-3)the Public Welfare Industry(Agriculture)Scientific Research of China(200903010-06)
文摘Good crop stand establishment and root system development are essential for optimum grain yield of dryland wheat (Triti-cum aestivumL.). At present, little is known about the effect of tilage and straw mulch on the root system of wheat under dryland areas in southwestern China. The aim of this study was to evaluate the effect of three tilage treatments (no-til, NT; rotary til, RT; conventional til, CT) and two crop residue management practices (straw mulch, ML; non-straw mulch, NML) on stand establishment, root growth and grain yield of wheat. NT resulted in lower soil cover thickness for the wheat seed, higher number of uncovered seeds, lower percentage of seedling-less ridges and lower tiler density compared to RT and CT; ML resulted in higher tiler density compared to NML. Straw mulching resulted in more soil water content and root length density (RLD) at most of the growth stages and soil depths. The maximum RLD, root surface area density and root dry matter density were obtained under NT. In the topmost 10 cm soil layer, higher RLD values were found under NT than those under RT and CT. There were no signiifcant differences in the yield or yield components of wheat among the tilage treatments in 2011–2012, but NT resulted in a signiifcant higher yield compared to RT and CT in 2012–2013. Grain yield was signiifcantly higher in ML compared to in NML. A strong relationship was observed between the water-use efifciency and the grain yield. Both NT and ML proved beneifcial for wheat in term of maintaining higher tiler density, better soil water status and root growth, leading to a higher grain yield and enhanced water-use efifciency, especialy in a low rainfal year.
基金financial supportprovided by the National Natural Science Foundation of China project, No. 311712494the financial support provided by NATP, BARC, Dhaka, Bangladesh
文摘Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of tillage(conventional and no-tillage system) and crop establishment methods(transplanting at a spacing of 20 cm × 20 cm with one seedling per hill and direct seeding at a seeding rate of 22.5 kg ha-1). Our results revealed that, at maximum tillering(Max.) and at maturity(MA) stages, direct seeding(DS) resulted in 22% more tillers than transplanting(TP) irrespective of tillage system. Tiller mortality reached a peak between panicle initiation(PI) and booting(BT) stages, and was 16% higher under conventional tillage(CT) than under no-tillage(NT). Transplanting required 29% more time for the completion of tillering and less for DS. Tillering rate was 43% higher in DS than TP under either CT or NT. There was a positive correlation between panicle number per m2and maximum tiller number per m2, but not panicle-bearing tiller rate. The panicle bearing tiller rate was higher under DS than TP and higher under NT than CT. Tiller dry weight gradually increased up to heading(HD) stage, and was 14% higher under TP than DS. Leaf area(cm2tiller-1) gradually increased from Max. to HD stage and then decreased by 34% in conventional tillage transplanting(CTTP) and 45% in no-tillage transplanting(NTTP) from 12DAH–24DAH(days after heading), but was similar(35%) under DS under either CT or NT. Grain yield was higher under CTTP owing to the larger sink size(heavier panicle, more spikelets in per cm length of panicle) than under DS.
基金supported by the Capital Medical Development and Scientific Research Fund(2009-1033)and the Science and Technology Plan of Beijing City(Z101107050210018)
文摘We established a diagnostic model to predict acute Mycoplasma pneumoniae (M. pneumonia) infection in elderly Community-acquired pneumonia (CAP) patients. We divided 456 patients into acute and non-acute M. pneumoniae infection groups. Binary logistic regression and receiver operating characteristic (ROC) curves were used to establish a predictive model. The following independent factors were identified: age 〉 70 years; serum cTNT level 〉 0.0S ng/mL; lobar consolidation; mediastinal lymphadenopathy; and antibody titer in the acute phase 〉 1:40. The area under the ROC curve of the model was 0.923 and a score of 2 7 score predicted acute M. pneumoniae infection in elderly patients with CAP. The predictive model developed in this study has high diagnostic accuracy for the identification of elderly acute M. pneumoniae infection.
基金This work was supported by the National Basic Research Program of China (Grant No. 2002CCA04100);the Key Science and Technology Program of Zhejiang Province, China (Grant No. 2003C34012);China Rice Science Foundation (Grant No. CRSF0003338);International Atomic Energy Agency (IAEA) Training Program.
文摘Elongations of coleoptile and mesocotyl are related directly to rice seedling establishment in soil and height of plant is related to lodging in rice production. Twelve typical rice cultivars with different lengths of coleoptile and mesocotyl (long, medium and short) were selected by screening the lengths of coleoptile and mesocotyl in 1500 accessions. The seedling establishments of these typical cultivars were compared under the combinations of different sowing depths and flooding durations, and two semi-dwarf varieties (G140, Zhong 96-21) with good seedling establishments and optimum mesocotyl lengths were found. The length of mesocotyl was completely fitted negative binomial distribution and the length of coleoptile was nearly fitted Iognormal distribution. Analysis of the relationships among mesocotyl, coleoptile, seeding depth, flooding duration, and their interactions to seedling establishment percentage showed that there existed significant relations among mesocotyl, coleoptile, mesocotyl x coleoptile, seeding depth, flooding duration and mesocotyl x sowing depth in the experiment for seedling establishment.
基金supported by the project of the National Natural Science Foundation of China(Grant No.41305098)the Chinese Academy of Sciences Strategic Priority Research Program(Grant No.XDA05110103)the National Natural Science Foundation of China(Grant No.41305096)
文摘Environmental changes are expected to shift the distribution and abundance of vegetation by determining seedling estab- lishment and success. However, most current ecosystem models only focus on the impacts of abiotic factors on biogeophysics (e.g., global distribution, etc.), ignoring their roles in the population dynamics (e.g., seedling establishment rate, mortality rate, etc.) of ecological communities. Such neglect may lead to biases in ecosystem population dynamics (such as changes in population density for woody species in forest ecosystems) and characteristics. In the present study, a new establishment scheme for introducing soil water as a function rather than a threshold was developed and validated, using version 1.0 of the IAP-DGVM as a test bed. The results showed that soil water in the establishment scheme had a remarkable influence on forest transition zones. Compared with the original scheme, the new scheme significantly improved simulations of tree population density, especially in the peripheral areas of forests and transition zones. Consequently, biases in forest fractional coverage were reduced in approximately 78.8% of the global grid cells. The global simulated areas of tree, shrub, grass and bare soil performed better, where the relative biases were reduced from 34.3% to 4.8%, from 27.6% to 13.1%, from 55.2% to 9.2%, and from 37.6% to 3.6%, respectively. Furthermore, the new scheme had more reasonable dependencies of plant functional types (PFTs) on mean annual precipitation, and described the correct dominant PFTs in the tropical rainforest peripheral areas of the Amazon and central Africa.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA05110103)the State Key Project for Basic Research Program of China(Grant No.2010CB951801)the National High Technology Research and Development Program of China(863 Program)(Grant No.2009AA122105)
文摘In Dynamic Global Vegetation Models (DGVMs), the establishment of woody vegetation refers to flowering, fertiliza- tion, seed production, germination, and the growth of tree seedlings. It determines not only the population densities but also other important ecosystem structural variables. In current DGVMs, establishments of woody plant functional types (PFTs) are assumed to be either the same in the same grid cell, or largely stochastic. We investigated the uncertainties in the competition of establishment among coexisting woody PFTs from three aspects: the dependence of PFT establishments on vegetation states; background establishment; and relative establishment potentials of different PFTs. Sensitivity experi- ments showed that the dependence of establishment rate on the fractional coverage of a PFT favored the dominant PFT by increasing its share in establishment. While a small background establishment rate had little impact on equilibrium states of the ecosystem, it did change the timescale required for the establishment of alien species in pre-existing forest due to their disadvantage in seed competition during the early stage of invasion. Meanwhile, establishment purely fiom background (the scheme commonly used in current DGVMs) led to inconsistent behavior in response to the change in PFT specification (e.g., number of PFTs and their specification). Furthermore, the results also indicated that trade-off between irtdividual growth and reproduction/colonization has significant influences on the competition of establishment. Hence, further development of es- tablishment parameterization in DGVMs is essential in reducing the uncertainties in simulations of both ecosystem structures and successions.
文摘Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the transition begins as early as in April, followed by abrupt change in May-June; the Asian summer monsoon situation is fully established in June. The winter convective center in Sumatra moved steadily northwestward across the "land bridge" of the maritime continent and the Indo-China Peninsula as time goes from winter to summer, thus giving rise to the change in large scale circulations that is responsible for the summer monsoon establishment over SE Asia and India; the South China Sea to the western Pacific summer monsoon onset bears a close relation to the active convection in the Indo China Peninsula and steady eastward retreat of the subtropical TBB high-value band,corresponding to the western Pacific subtropical high.
基金supported by the Occupational Health Standards Program (20100304) from Ministry of Health of China
文摘This study aims to establish the occupational exposure limit (OEL) in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring. The clinical therapeutic dose was used as lowest observed effect level (LOEL), and no observed effect level (NOEL) was achieved by using a safety factor. The highest concentration of warfarin monitored in the worksite of centrifuge washing, drying and packing were 0.029 mg/m3, 0.052 mg/m3 respectively, which did not exceed the OEL 0.2 mg/m3 recommended by NIOSH and ACGIH. Considering its feasibility for enforcement and protection for workers, we recommend OEL 0.2 mg/m3 of warfarin in China.
基金This work was supported by grants from the National Natural Science Foundation of China(Grant Nos.31861143006,31901483 and 31770195)National Key Research and Development Program of China(Grant No.2016YFDO101801)Zhejiang Provincial‘Ten Thousand Talent Program’(Grant No.2018R52025).
文摘An ideal leaf shape is beneficial to the yield of rice.Molecular understanding of the leaf primordia and polarity establishment plays a significant role in exploring the genetic regulatory network of leaf morphogenesis.In recent years,researchers have cloned an array of coding genes and a few non-coding small RNAs involved in rice leaf development through regulating the development of leaf primordia,vascular bundles,sclerenchyma cells,bulliform cells,cell walls and epidermis cells.These genes and their interactions play critical roles in rice leaf development through the determination and regulatory role in gene expression,and their coordination with other genetic networks or signal pathways.But the relationship among these genes is poorly defined and the underlying network is still unclear.In this review,we introduced the regulatory pathways of leaf primordium development and leaf polarity establishment,mainly the relationship between cell development mechanism and leaf polarity establishment,focusing on how leaf tissue affects leaf shape.Hopefully,the regulation network reviewed here has immediate implications for future research and genomic design breeding.
基金supported by Science and Technology Project of Henan Provincial Department of Health(NO:2011020038)
文摘Objective:To induce pluripotent stem(IPS)cells from fibrocytes that are separated from liver cancer patients.Methods:The fibrocytes were reprogrammed to IPS cells by lentiviral vector,stained and identified by immunohistochemistry.Results:The IPS cells were successfully established from fibrocytes after infection,and IPS cell clones formed in round shape under a microscopy.The induction rate was 0.013%±0.007%.No tumor formed at the back of nude mice within 8 weeks after the inoculation of cell clone.However,tetatoma appeared in nude mice within 1 week after IPS inoculation.A few tumors formed in nude mice within 4 weeks after the inoculation of cell clones.However,subcutaneous tumors formed within 1 week after IPS inoculation.The induced IPS cells showed three germ layers in tetatoma.Nanog and OCT4 in the induced IPS cells showed hypomethylation.SSEA-A,TRA-1-6-,TRA-1-81 and Nanog were highly expressed in the induced IPS cells,indicating the IPS cells possessed the similar ability as the stem cells.Conclusion:The IPS cells of liver cancer patients can be established effectively from fibrocytes and can be cultured stably in vitro,which provides an approach for the treatment of intermediate or advanced stage liver cancer.
基金supported by the National Natural Science Foundation of China(No.81921002,No.81900970)Young Physician Innovation Team Project(No.QC202003)from Ninth People’s Hospital,Shanghai Jiao Tong University School of MedicineShanghai Sailing Program(19YF1426000)jointly。
文摘Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are still not fully understood.Animal models can be used to simulate the pathophysiological features of MRONJ,serving as standardized in vivo experimental platforms to explore the pathogenesis and therapies of MRONJ.Rodent models exhibit excellent effectiveness and high reproducibility in mimicking human MRONJ,but classical methods cannot achieve a complete replica of the pathogenesis of MRONJ.Modified rodent models have been reported with improvements for better mimicking of MRONJ onset in clinic.This review summarizes representative classical and modified rodent models of MRONJ created through various combinations of systemic drug induction and local stimulation and discusses their effectiveness and efficiency.Currently,there is a lack of a unified assessment system for MRONJ models,which hinders a standard definition of MRONJ-like lesions in rodents.Therefore,this review comprehensively summarizes assessment systems based on published peer-review articles,including new approaches in gross observation,histological assessments,radiographic assessments,and serological assessments.This review can serve as a reference for model establishment and evaluation in future preclinical studies on MRONJ.
基金supported by Zhejiang Provincial Population and Family Planning Foundation of China (N20100011)
文摘Venereal diseases are considered to be the most prevalent infectious diseases in the worldwide. China is now faced with a year-by-year increasing incidence of sexually transmitted diseases (STD), which are spreading from high-risk groups to the general population. Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum and herpes simplex virus-2 (HSV-2) are always regarded as the most common venereal pathogens. The "golden standard" for testing Neisseria gonorrhoeae remains to be bacteria culture or microscopic examination.
基金the project ‘Resource Conservation Technologies for Sustainable Development of Agriculture’
文摘In recent years, conventional rice production technologies have been leading to deterioration of soil health and declining farm profitability due to high inputs of water and labor. Conservation agriculture (CA) based resource-conserving technologies i.e. zero-tillage (ZT), raised-bed planting and direct-seeded rice (DSR) have shown promise as alternatives to conventional production technologies to overcome these problems. Present study was undertaken during 2009-2012 to establish an understanding of how permanent raised bed cropping system could be practiced to save water at the field application level to improve water productivity and also have the capability to enhance productivity, profitability and soil physical quality. The results showed that among different crop establishment techniques, conventional-tilled puddle transplanted rice (CT-TPR) required 14%-25% more water than other techniques. Compared with the CT-TPR system, zero till direct-seeded rice (ZT-DSR) consumed 6%-10%less water with almost equal system productivity and demonstrated higher water productivity. Wide raised beds saved about 15%-24% water and grain yield decrease of about 8%. Direct-seeded rice after ZT or reduced tillage or on unpuddled soil provided more net income than CT-TPR. The CT-TPR system had higher bulk density and penetration resistance due to compaction caused by the repeated wet tillage in rice. The steady-state infiltration rate and soil aggregation (〉 0.25 mm) were higher under permanent beds and ZT and lower in the CT-TPR system. Under CT-TPR, soil aggregation was static across seasons, whereas it improved under no-till and permanent beds. Similarly, mean weight diameter of aggregates was higher under ZT and permanent beds and increased over time. The study reveals that to sustain the rice productivity, CA-based planting techniques can be more viable options. However, the long-term effects of these alternative technologies need to be studied under varying agro-ecologies in western Uttar Pradesh, India.
文摘We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.
基金supported by National Science Foundation of China (5 98780 33) Young Science Foundation of Tianjin- 2 1Centuries(97370 5 111)
文摘According to the principle of belt seeding establishment of adhesive bonded fabric,applied indexes of belt seeding establishment effect were studied using carrier combination of several kinds of waste meterials under soil cover.The results showed that the effect depended on the coordinated characteristics of carrier combination from turfgrass and waste materials.It was testified that production of belt seeding was feasible using a certain kind of waste material carrier under given conditions.The study provided a scientific basis for applying evvironmental engineering to belt seeding establishment of waste materials and developing nwe industry of environmental protection.
基金Supported by Science and Technology Open Cooperation Project of Henan Province(162106000017)Science and Technology People-benefiting Plan Project of Henan Province(152207110004)+1 种基金Major Science and Technology Program of Henan Province(16110051020)Puyang Science and Technology Plan Project(160215)
文摘In order to establish an HPLC-MS/MS method for detecting progesterone in raw milk,raw milk was extracted with methanol,and then determined by HPLC-MS/MS after the purification with a C18 solid phase extraction column. Qualitative determination was performed according to retention time and selective ion abundance ratio,and quantification was performed by external standard method. The results showed that progesterone had a good linear relation in the range of0. 1-5. 0 ng/ml,with a correlation coefficient of 0. 999 9. The detection limit of the established method was 0. 02 μg/kg,and the quantification limit was0. 04 μg/kg. In the standard addition range of 0. 1-5. 0 ng/ml,the recovery ranged from 79. 6% to 105. 1%,with relative standard deviation≤8. 9%. Compared with the industry standard,the detection limit and the quantification limit of the established method are significantly reduced,and the sensitivity of the method is enhanced,so this method is suitable for the detection of progesterone residue in fresh milk in pastures.
文摘Field experiments with medicinal rice Njavara were conducted at Cropping Systems Research Centre, Karamana, Thiruvananthapuram, Kerala during summer of 2007 and 2008. The experimental design was split plot with three replications. The treatments consisted of four establishment techniques viz., System of Rice Intensification SRI (M1), Integrated Crop Establishment Method (ICM) (M2), Package of practices (PoP) (M3) of Kerala Agricultural University and Conventional Management Practices (CMP) (M4) in main plot. Three nutrient sources viz., (1) organic, 2) integrated use of organic and inorganic, 3) chemical fertilizers only) were used under different establishment techniques. Root dry matter production was determined at weekly intervals and plant nutrients uptake was determined by calculating from the product of dry matter, straw dry weight, grain yield and percentage of nutrients. Results revealed that at early stages (4-6 WAT/WAS) (Weeks after transplanting/Weeks after sowing), root dry matter production hill-1 was in the order of SRI > ICM > CMP > PoP which changed to SRI > ICM > PoP > CMP from 7th week onwards. Maximum root dry matter (at 9th WAS/WAT) was recorded in SRI (0.51/0.50 g.hill-1 in 2007/2008). The total nutrients uptake (N, P, K, Fe, Mn and Zn) in conventional management practices was significantly higher than other establishment techniques. Among the nutrient sources, higher total (N, P, K, Fe, Mn and Zn) uptake by crop was recorded under integrated nutrient source than organic and inorganic sources.