Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dy...Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dynamics.Herein,inspired by the cellular machinery of reversible thiol modification by NO,we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics.展开更多
基金supported by the National Natural Science Foundations of China(nos.22077112,21778048,81673489,31871414,and 81125023)Natural Science Foundation of Zhejiang Province,China(no.LR18H300001)National Science and Technology Major Project“Key New Drug Creation and Manufacturing Program”(nos.2018ZX09711002-010-004,2018ZX09711002-007-002,2019ZX09201001-003-009,2019ZX09201001-003-010,and 2019ZX09201001-004-010),K.C.Wong Education Foundation,and Singapore University of Technology and Design(SUTD)[SUTD-ZJU IDEA grant nos.T1SRCI17126 and SUTD-ZJU(VP)201905].
文摘Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dynamics.Herein,inspired by the cellular machinery of reversible thiol modification by NO,we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics.
