Research Overview of Zhuang Medicine Fumigation Lotions

At present,there is little research on the application of Zhuang medicine fumigation lotions in skin diseases,and there is no research on related preparation processes and quality standards.In this paper,the characteristics of its formulation and the research progress in skin diseases are reviewed,in order to deepen the understanding of Zhuang medicine fumigation lotions and provide a more detailed theoretical basis for its clinical research,development and application.

Comprehensive analysis of the chemical constituents in sulfur-fumigated Lonicerae Japonicae Flos using UHPLC-LTQ-Orbitrap mass spectrometry

Lonicerae Japonicae Flos(LJF),a kind of traditional Chinese medicines(TCMs),has functions of detoxifying and evacuating heat.In the study,a method based on ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS)was developed for the chemical constituent analysis of organic acids,flavonoids,iridoids and new-generated compounds in sulfur-fumigated LJF(SF-LJF).Based on the accurate mass measurement(<±5 ppm),chromatographic behavior and diagnostic product ions(DPIs),113 constituents were unambiguously or tentatively characterized from SFLJF extract,including 46 chlorogenic acids,19 flavonoids,29 iridoid glycosides and 19 newly-generated compounds(including 17 sulfur-containing derivatives).In addition,5-CQA(5-caffeoylquinic acid,chlorogenic acid)was chosen to be sulfur-fumigated for the result validation.It was found that the most significant change of LJF after sulfur fumigation was the occurrence of sulfate or sulfite esterification reactions,which resulted in the emergence of many new sulfur-containing components.Our results demonstrated that the established method was a useful and efficient analytical tool to comprehensively characterize the material basis of SF-LJF,and also an excellent guidance of quality control about LJF.

Insecticidal Potential of α-Pinene and β-Caryophyllene against Myzus persicae and Their Impacts on Gene Expression

Myzus persicae(M.persicae)is now considered a threat to agricultural crops due to economic losses.Numerous synthetic insecticides applied every year against M.persicae,are reported to be unsafe for environment,humans,and beneficial insects.Furthermore,several species of Myzus have been found to develop resistance due to over application of these insecticides.Therefore,it is required to find some novel insecticide that would be safe for the environment as well as for humans.In the current study,two major pure constituentsα-pinene andβ-caryophyllene were evaluated for their insecticidal potential against M.persicae using a fumigant toxicity assay.Furthermore,impact ofα-pinene andβ-caryophyllene on expression of five different genes,e.g.,HSP 60,FPPS I,OSD,TOL and ANT responsible for reproduction,dispersion,and growth of M.persicae has also been investigated.To perform fumigant toxicity assay,five different concentrations(3.5,4,4.5,5 and 6μL L−1)ofα-pinene andβ-caryophyllene were prepared.Lethal concentration(LC)was calculated,and gene expression studies were executed through qRT PCR at LC30 ofα-pinene andβ-caryophyllene.Both constituents demonstrated excellent fumigant toxicity effects against M.persicae at all five concentrations.However,α-pinene shows significantly better results(98%)as compared toβ-caryophyllene(80%)after 72 h at 6μL L−1 of dose.The highest upregulation in expression was demonstrated at LC30 dose ofα-pinene in five in three out of five genes understudy(TOL,ANT,and FPPS I).Conversely,two genes HSP 60 and OSD demonstrated downregulation at LC30 dose ofβ-caryophyllene.Conclusively,our results highlighted the promising insecticidal potential of both compoundsα-pinene andβ-caryophylleneby interfering with the reproduction and development related processes in M.persicae,allowing us to recommend the phytoconstituents under investigation as an ecofriendly alternative to synthetic insecticides.

Extraction Process of Zhuang Medicine Fumigation Lotion

[Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amount of water added,decocting time and extraction times as factors,and syringin content and dry extract yield as indexes.The content of syringin was determined by high performance liquid chromatography.[Results]The best water extraction process was:soaking in water for 1 h,decocting twice,added 10 times the amount of water each time,decocting for 1 h.The average content of syringin in 3 batches was 0.98 mg/g,and the average dry extract yield was 26.07%.[Conclusions]The project adopts water extraction method to prepare Zhuang medicine fumigation lotion,which has the characteristics of high efficiency and suitable for large-scale production.The quality control method is reliable,rapid and accurate,and can effectively control the quality of the lotion.

储藏物害虫嗜卷书虱对DDVP熏蒸的行为反应与致死剂量

1 引言 嗜卷书虱(Liposcelis bostrychophila)属啮虫目虱啮科.其体形微小,食性复杂.栖息场地多在粮食仓库及食品加工厂、图书馆、标本馆等地.大量发生时可造成严重损失,传播微生物,并以其尸体、排泄物等污染储藏的粮食和其它储藏物品[7].王进军等[15]调查发现,在我国"双低"和"三低"贮粮的粮仓中.书虱已成为优势种群,对贮粮安全构成了威胁.

Expression of S100B during the innate immune of corneal epithelium against fungi invasion

AIM： To explore the expression of SIOOB in corneal epithelial cells under ,Aspergillus stimulation both in vivo and in vitro. METHODS： Immortalized human corneal epithelial cells （HCECs） were exposed to inactive #lsperg///us fumigatus （A. fumigatus） conidia at 0, 4, 8, 12, 16, and 24h respectively. The corneas of Wistar rats were exposed to active A. fumigatus at 0, 12, 24, 48h and the normal rat corneas were used for normal control. The mRNA level of S100B was evaluated by real time quantitative reverse transcription-polymerase chain reaction （qRT-PCR）. Sl00B protein expression in cornea epithelium was detected by immunohistochemical/immunocytochemical staining （IHC/ICC）. RESULTS： Histopathology revealed a significant inflammatory cell infiltration in fungal keratitis human and rat cornea. Corneal epithelial cells didn＇t express or rarely express S100B at baseline. A. fumigatus significantly induced S100B mRNA expression in cultured corneal epithelial cells in a time depended manner in vitro the mRNA began to rise significantly at 8h in vitro （P〈0.05） and continue to rise as time prolonged （P〈0.01）. in vivo S100B mRNA level was low in the normal corneas. However, it was increased in keratitis corneas from 12h after infection （P〈0.05） and reached to a peak at 24h （P〈0.001）. Immunochemistry revealed an obvious staining in fungal keratitis corneas as well as immortalized HCECs compared to the normal ones respectively, indicating an increased expression of SlOOB protein. CONCLUSION： S100B exists in corneal epithelial cells and is over-expressed under A. fumigatus stimulation. Sl00B may play an important role in the innate immune response of the corneal epithelium during A. fumigatus infection.

Effect of Ammonium Fixation on Determination of N Mineralized from Soil Microbial Biomass

Two soils with relatively high (Soil 1) and low (Soil 2) ammonium fixation capacities were used in thisstudy to extalne the effect of ammonium fixation on the determination of N mineralised from soil ndcrobialbiomass. organism suspellsioll was quantitatively introduced to Soil 1 at various rates. Both fumigation-incubation (FI) and fumigation-ext raction (FE ) met hods were used to t reat t he soil. The amount of ffeedNH4+-N increased with increasing rate of organism-N addition. A close correlation was found between theamoun of fixed aznmonium and the rate of organism-N addition. The net increso of fixed NH4+-N wereequivalent to 38% and 12% of the added organism-N for FI and FE treatments, respectively in this specificsoil. To provide isotopic evidence, 15N-labelled organism-N was added to Soils 1 and 2 at 121.4 mg N kg-1.In FI treatment, 22 and 3 mg N kg-1 of labelled N were found in the fraction of fixed NH4+-N in Soils 1 and2 respectively; while in FE treatment, 9 mg N kg-1 of labelled N was found in the fraction of fixed NH4+-Nin Soil 1 only. There was no labelled N in the fraction of fixed NH4+-N in Soil 2. In all of the unfumigated(check) soils, there was little or no labelled N in the fixed fractions, probably because the organism-N addedwas easily mineralized and nitrified. A mean of 0.64 for KN value, the fraction of N ndneralized in the killedmicrobial biomass, was obtained with inclusion of the net increase of fixed NH4+-N. The corresponding valuecalculated with exclusion of the net increase of fixed NH4+-N was 0.46. It was concluded that ammniumfixation was a problem in determination of KN, particularly for soils with a high ammonium fixation capacity.Results also showed that microbial biomass N measurement by FE method was less affected by ammoniumprocess than that by FI method.

Early expression of PTX3 in Aspergillus fumigatus infected rat cornea

AIM： To investigate the expression of pentraxin 3（PTX3） in rat corneal epithelium at the early stage of Aspergillus fumigatus（A. fumigatus） infection. METHODS： A total of 50 Wistar rats were randomly divided into control group, Sham group and experimental group（fungal keratitis group, FK group）. The right eye was chosen as the experiment one and infected by A. fumigatus. Rats were executed at 8, 16 and 24 h after the experimental models being established. Corneal epithelia were collected to assess the expression of PTX3 by quantitative reverse transcription polymerase chain reaction（q RT-PCR） and Western blot analysis. RESULTS： Corneal inflammation scores increased as infection prolonged（P〈0.05, P〈0.001）. PTX3 m RNA expression was low in normal and Sham group rats＇ corneas. Level of PTX3 m RNA in infected rat cornea was elevated at 8 h and peaked at 16 h. The difference was significant compared with control group（P〈0.001）. Western blot analysis also showed a significant increase of PTX3 protein in experimental group at 8 h and peaked at 16 h（P〈0.001）. The synchronous expression of control group and experimental group were also in significant difference（P〈0.001）. CONCLUSION： PTX3 exists in cornea epithelium and is significantly increased after A. fumigatus infection. PTX3 plays an important role in the early stage of cornea innate immunity against A. fumigatus.

Microwave-assisted Extraction and Biological Activity Determination of Insecticidal Components of Seleng Wormwood(Artemisia selengensis Turcz.)

[Objective] The aim was to study the biological activity of insecticidal components of Seleng Wormwood on cabbage butterfly （Pieris rapae）.[Method] With the assisted condition of microwave heating,ethanol,ethyl ether,water and methylbenzene were selected as solvents to extract Seleng Wormwood,and the antifeedant,stomach toxicity,contact toxicity and fumigation activities of various solvent extracts against cabbage butterfly were determined.[Result] Various solvent extracts all showed certain antifeedant,stomach toxicity,contact toxicity and fumigation activity against the larvae of cabbage butterfly,and the biological activity of ethanol extract was the strongest.[Conclusion]The results could provide reference for development and utilization of botanical pesticides of Seleng Wormwood.

Frankincense,a Special Spice Walking Along the Silk Road

Frankincense is also called“Fumigated land,”“Horsetail spice,”and“Godsend spice.”The alias“Fumigated land”shows its strong fragrance that can cover the whole land.The name of“Horsetail spice”implies its morphologic characteristics and mild effects,and another name of“Godsend spice”indicates its functions of relieving swelling and pain,healing sore,and growing muscle,as if it is the grace donated by God.The name of“Frankincense”displays its complex productive progress.With thick quality and strong fragrance,frankincense acts as daily supplies for religious activities and folk activities due to its low price and a wide range of uses in medicine and daily life.Frankincense shows the infinite charm of spice culture along the Silk Road from the distant Western Regions to the East.At the same time,it enriches the treasure house of traditional Chinese medicine and becomes one of the most popular spices in both eastern and western,nobility and common.

Relationship among Different Soil Biochemical Methods to Determine Soil Health

Numerous soil biochemical methods are used to determine the soil health status, but the relationships among these methods are not well understood. Relationships among soil biochemical tests, 1) chloroform fumigated microbial biomass C (CFMBC), 2) permanganate oxidizable C (POXC), 3) Solvita CO2-burst (Solvita), 4) Solvita labile amino nitrogen (SLAN), and short-term soil CO2 efflux during laboratory incubation using (v) Alkali-base trap (Alkali) and (vi) infrared gas analyzer (IRGA), were evaluated for nine agricultural soils collected across the Red River Valley of North Dakota and Minnesota, USA. Not a single test is comprehensive to relate with all soil biochemical tests. Coefficient of variation percentage for particular method varied with soil type. Among six tests, CFMBC is significantly (p < 0.05) related with Alkali (r = 0.37), Solvita (r = 0.57), SLAN (r = 0.52), and POXC (r = 0.68). Soil CFMBC correlates with most of soil biochemical tests and can be potential to determine soil biochemical condition.

Killing Effect of Mixed Fumigation of Phosphine and Carbon Dioxide on Eggs of Lasioderma serricorne F.

[Objective] This study aimed to investigate the killing effect of mixed fumi- gation of phosphine and carbon dioxide on eggs of Lasioderma serricorne F. [Method] The outside-storage phosphine generator was placed in a tabernacled smoke box, and the mortality rates of L. serricome F. eggs in the smoke box under conditions of different aluminium phosphide usage amount and different fumigation time were studied. In addition, the times needed by tabernacle and smoke box cen- ter to reach the phosphine concentration peak were recorded. [Result] The optimum conditions for killing the eggs of L. serricorne were as follows： temperature of （27± 2） ℃, relatively humidity of （45±5）%, aluminium phosphide usage amount of 1.5 g/m3 and effective exposure time of 96 h. The killing effect of mixed fumigation of phos- phine and carbon dioxide was increased with the extension of fumigation time. The increased usage amount of aluminium phosphide showed no significant effect on killing effect. The tabernacle space and smoke box center all required relatively short time to reach the phosphine concentration peak. If the tabernacle had a good airtightness, the overall fumigation time could be shortened. [Conclusion] The fumi- gation method is reliable, and it can be used for the control of L. serricorne F. in tobacco storage.

Impact of Soil Fumigation Practices on Soil Nematodes and Microbial Biomass

This study was designed to understand the impact of methyl bromide (MB) (CH3Br) and its alternatives on both free-living and root-knot nematodes in the soil. A randomized complete block experiment with six treatments and 4 replicates (each replicate in a separate greenhouse) was established in Qingzhou, Shandong Province, China. In addition to MB and untreated control (CK) treatments there were four alternative soil fumigation practices including MB+virtually impermeable films (VIF), metam sodium (MS), MS +VIF and soil solarization combined with selected biological control agents (SS+BCA). Two tomato (Lycopersicum esculentum Mill.) cultivars, cv. Maofen-802 from the Xian Institute of Vegetable Science, China, and cv. AF179 Brillante from the Israeli Hazera Quality Seeds, were selected as test crops. The results indicated that Rhabditidae was the most dominant population with percentage abundance as high as 85% of the total number of identified free-living nematodes, followed by that of Cephalobidae. Methyl bromide and its alternatives except for the non-chemical SS+BCA treatment controlled the target pest, root-knot nematodes. Also, the impact of the three chemical alternatives on free-living nematode number and functional group abundance was similar to the impact associated with a typical methyl bromide application. Chemical fumigation practices, especially that with MB, significantly reduced the number of nematodes in the soil and simultaneously significantly reduced the number of nematode genera thereby reducing nematode diversity. All the four soil chemical fumigation activities decreased soil microbial biomass and had an obvious initial impact on microorganism biomass. Furthermore, both plant-parasitic and fungivore nematodes were positively correlated with soil microbial biomass.

Anaerobic soil disinfestation:A chemical-independent approach to pre-plant control of plant pathogens

Due to increasing regulations and restrictions, there is an urgent need to develop effective alternatives to chemical-dependent fumigation control of soilborne pests and pathogens. Anaerobic soil disinfestation （ASD） is one such alternative showing great promise for use in the control of soilborne pathogens and pests. This method involves the application of a carbon source, irrigation to field capacity, and covering the soil with a plastic tarp. While the mechanisms of ASD are not completely understood, they appear to be a combination of changes in the soil microbial community composition, production of volatile organic compounds, and the generation of lethal anaerobic conditions. The variety of materials and options for ASD application, including carbon sources, soil temperature, and plastic tarp type, influence the efficacy of pathogen sup- pression and disease control. Currently, both dry （e.g., rice bran） and liquid （e.g., ethanol） carbon sources are commonly used, but with different results depending on environmental conditions. While solarization is not an essential component of ASD, it can enhance efficacy. Understanding the mechanisms that mediate biological changes occurring in the soil during ASD will facilitate our ability to increase ASD efficacy while enhancing its commercial viability.

Evaluation of Vaporized Hydrogen Peroxide Fumigation as a Method for the Bio-decontamination of the High Efficiency Particulate Air Filter Unit

Objective To evaluate the performance of vaporized hydrogen peroxide （VHP） for the bio-decontamination of the high efficiency particulate air （HEPA） filter unit. Methods Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.5 h submergence in eluent followed by 200- time violent knocks. Results Due to the presence of HEPA filter in the box, spore recovery from coupons placed at the bottom of the filter downstream was significantly higher than that from coupons placed at the other locations. The gap of decontamination efficiency between the top and the bottom of the filter downstream became narrower with the exposure time extended. The decontamination efficiency of the bottom of the filter downstream only improved gently with the injection rate of H202 increased and the decontamination efficiency decreased instead when the injection rate exceeded 2.5 g/min. The commercially available bioindicators were competent to indicate the disinfection efficiency of VHP for the HEPA filter unit. Conclusion The HEPA filter unit is more difficult than common enclosure to decontaminate using VHP fumigation. Complete decontamination can be achieved by extending fumigation time. VHP fumigation can be applied for in-situ biodecontamination of the HEPA filter unit as an alternative method to formaldehyde fumigation.

Fumigant and repellent properties of sesquiterpene-rich essential oil from Teucrium polium subsp,capitatum(L.)

Objective:To test fumigant and repellent properties of sesquiterpene—rich essential oil from Teucrium polium subsp.capitation(L.).Methods:The fumigant toxicity test was performed at(27±1)℃.(65± 5)%relative humidity,and under darkness condition and 24 h exposure time.The chemical composition of the isolated oils was examined by gas chromatography—mass spectrometry.Results:The major compounds were α-cadiuol(46.2%),earyophyllene oxide(25.9%),α muurolol epi(8.1%),cadalene(3.7%) and longiverbenone(2.9%).In all cases,considerable differences in mortality of insect to essential oil vapor were observed in different concentrations and exposure times.Callosobruchus maculatus(C.maculates)(LC_(50)=148.9μL/L air) was more susceptible to the tested plant product than Teucrium castaneum(T.castaneum)(LC_(50)=360.2μL/L ain based on LC_(50)values.In the present investigation,the concentration of 3μ L /mL acetone showed 60% and 52% repellency against T.casteneum and C.maculatus adults,respectively.Conclusions:The results suggests that sesquiterpene—rich essential oils from the tested plant could be used as a potential control agent for stored—product insects.

Application of the combination of 1,3-dichloropropene and dimethyl disulfide by soil injection or chemigation: effects against soilborne pests in cucumber in China

The combination of 1,3-dichloropropene＋dimethyl disulfide （1,3-D＋DMDS）, which forms a pre-plant soil fumigant, can provide a substitute for the environmentally unfriendly methyl bromide （MB）. Three greenhouse trials were performed to evaluate the root-knot nematode and soilborne fungi control efficacy in the suburbs of Beijing in China in 2010-2014. Randomized blocks with three replicates were designed in each trial. The combination of 1,3-D＋DMDS （10＋30 g m-2） significantly controlled Meloidogyne incognita, effectively suppressed the infestation of Fusarium oxysporum and Phytophthora spp., and successfully provided high commercial fruit yields （equal to MB but higher than 1,3-D or DMDS）. The fumigant soil treatments were significantly better than the untreated controls. These results indicate that 1,3-D＋DMDS soil treatments can be applied by soil injection or chemigation as a promising MB alternative against soilborne pests in cucumber in China.

Impact of Oriental Fruit Fly Postharvest Treatments on Avocado

The detection in August 2015 of the Oriental Fruit Fly (Bactrocera dorsalis Hendel, Diptera: Tephritidae) in the Redland area in Miami-Dade County, Florida triggered a quarantine that restricted the movement of fruit fly host material in an approximately 99-square mile (256-square kilometer) area. The quarantine affected 4000 acres of fruit bearing commercial avocado groves. Approved post-harvest treatments for B. dorsalis and avocado included in the USDA Treatment Manual were acceptable for immediate certification and movement of fresh avocados from the quarantine area. However, it was unknown if Florida avocados would meet quality standards (US combination grade) after the treatments. Three post-harvest treatments that combine methyl bromide fumigation and cold storage periods were tested on six avocado varieties from Florida. The treatments differed in the durations of the fumigation and cold treatment periods. A seven day transit period at 8.3°C (47°F) was added to account for the time when the fruit leaves the packing house until it is sold by retailers. None of the six varieties had met the US combination grade after the treatments and transit period. Treated fruit exhibited both internal (pulp) and external (skin) damage. Damage was attributed to the fumigation component of the treatment, but the six varieties tolerated the cold portion of the treatment. Damage by fumigation ranged from 26% - 100%. In general, the longer the fumigation period the worse the effect. The need for alternative post-harvest treatments for Florida avocados is discussed.

Efficacy of combining traditional Chinese medicine fumigation with Western medicine for diabetic peripheral neuropathy:A systematic review and meta-analysis

Objective:A systematic review and meta-analysis was conducted to evaluate the effectiveness of supplementing Western medicine with Traditional Chinese medicine(TCM)fumigation in patients with diabetic peripheral neuropathy.Methods:The China Biomedical Literature,Chinese full-text periodical,China National Knowledge Infrastructure,WanFang,PubMed,EMbase,and Cochrane Library databases were searched for relevant randomized controlled trials published from inception through May 2015.The methodological quality of eligible studies was evaluated using the Cochrane Risk Bias Assessment tool,and summarized effects were calculated using Reviewer Manager 5.1 software.Subgroup analysis was conducted based on the duration of intervention.Results:The initial search identified 312 relevant studies,of which 40 randomized controlled trials involving 3497 patients were eligible for analysis.The results indicated that TCM fumigation significantly improved the curative effects[risk ratio(RR)=1.34,95%CI(confidence interval):1.29e1.39],common peroneal motor nerve conduction velocity[standard mean difference(SMD)=2.93,95%CI:2.26e3.61],common peroneal sensory nerve conduction velocity(SMD=2.23,95%CI:1.46e3.01),and plasma viscosity(SMD=1.02,95%CI:1.35e0.69)compared to Western medicine alone(all p<0.01).A subgroup analysis indicated that the curative effects were significant after 15 days(RR=1.31,95%CI:1.21e1.42),30 days(RR=1.33,95%CI:1.26e1.40),and 60 days(RR=1.50,95%CI:1.32e1.71)of combined treatment(all p<0.01).

Fungitoxicity of Methyl Iodide, Sulfuryl Fluoride, and Methyl Bromide to Ceratocystis fagacearum in Red Oak, Maple, Poplar, Birch and Pine Wood

The threat of wood-inhabiting fungi to American hardwood forests, lumber industries, and tourism has enormous eco-nomic significance, and the aesthetic and dollar values of properties are potentially disastrous. The efficacy of methyl iodide (MeI) and sulfuryl fluoride (SF) for eradicating wood-inhabiting fungus, Ceratocystis fagacearum was assessed in wood blocks of birch, maple, poplar and red pine based on in-vitro experiments. In a series of replicated controlled experiments, wood blocks were inoculated with a 1g macerated mycelium/spores mixture of C. fagacearum and fumigated with 160 and 240 g/m3 of MeI, SF and methyl bromide (MeBr) as control) for 24, 48, and 72 hours. Analysis of variance showed that fumigant types, fumigant concentrations, and exposure time as well as their interactions (C x T) had an effect on C. fagacearum recovery on tested wood species. Colonization of birch, maple, red pine, and poplar by C. fagacearum was significantly greater in non-fumigated samples than fumigated samples. C. fagacearum was greatly inhibited by MeI than SF in all wood species tested. Overall, the C x T products of ≤ 4.108 g-h/m3 for MeI and ≤ 8.755 g-h/m3 for SF were not effective in killing the fungus. These results suggest that longer treatment exposure time might achieve the goal of complete eradication of C. fagacearum and imply that MeI performed as well as MeBr in killing the fungus in some wood species by exposure time combination. Overall, MeI was most effective in killing the fungus than SF under the conditions of this study with potential implications for quarantine use.