Effects of feeder layer and BRL conditioned medium on mouse embryonic stem cells

In vitro growth and maintenance of embryonic stem (ES) cell lines derived from ICM cells of various blastocysts of 129 strain mice, the sustenance of their pluripotency and normal karyotype depend on the feeder layer of mouse embryonic fibroblasts (MEF). Compared with the feeder layer of MEF cells, medium conditioned by Buffalo rat liver cells (BRL-CM) is able to maintain pluripotency and karyo-typic normality of ES cells only in short term cell propagation. Besides, ES cells grown in BRL-CM are also capable of aggregation with 8-cell embryos of Swiss strain and develop into germ line chimaeras. Modification to the method of aggregating ES cells with early embryos by making a hole in agar layer on the top of MEF feeder cells was shown to be more convenient and efficient than the conventional microdrop method.

On an Application of the Improved Maximum Product Criterion to Inverse Acoustic Scattering in a Layered Medium

In this paper, we consider the numerical treatment of an inverse acoustic scattering problem that involves an impenetrable obstacle embedded in a layered medium. We begin by employing a modified version of the well known factorization method, in which a computationally effective numerical scheme for the reconstruction of the shape of the scatterer is presented. This is possible, due to a mixed reciprocity principle, which renders the computation of the Green function at the background medium unnecessary. Moreover, to further refine our inversion algorithm, an efficient Tikhonov parameter choice technique, called Improved Maximum Product Criterion (IMPC) is exploited. Our regularization parameter is computed via a fast iterative algorithm which requires no a priori knowledge of the noise level in the far-field data. Finally, the effectiveness of IMPC is illustrated with various numerical examples.

Occam Inversion of Transient Electromagnetic Data in a Layered Medium with Azimuthal Anisotropy

In recent years, the anisotropic study has become a hot topic in the field of electromagnetics. Currently, inversion technologies of transient electromagnetic sounding data are mainly based on the case of an isotropic medium. However, the actual underground electrical structure tends to be complicated and anisotropic. It is often found that the isotropic inversion technologies do not lead to good results for field transient electromagnetic sounding data. We have developed an algorithm for calculating the transient electromagnetic response in a layered medium with azimuthal anisotropy. An occam inversion algorithm has also been implemented to invert the transient electromagnetic data induced by a grounded horizontal electric dipole in a layered medium with azimuthal anisotropy. Synthetic examples demonstrate the stability and validity of the inversion algorithm. Experimental results show different data for inverting have great influence on the inversion results.

Electromagnetic Scattering in a Two-layered Medium

The object of this paper is to investigate the three-dimensional electro- magnetic scattering problems in a two-layered background medium. These problems have an important application in today＇s technology, such as to detect objects that are buried in soil. Here, we model both the exterior impedance problem and the inhomogeneous medium problem in R3. We establish uniqueness and existence for the solution of the two scattering problems, respectively.

The Near-Field Microwave Focusing in the Layer Mediums

This paper deals with the microwave focusing in the layer mediums near the antenna aperture. Based on the plane-wave spectrum theory, the relationship between the Hertz potential and the electromagnetic field is discussed. An approach to the description of the field distribution in the layer mediums in case of an arbitary field source is proposed. Given the focusing current distribution over the antenna aperture, the field distribution of the focal region is worked out. In describing the field distribution over the focal region, the integration of spectrum functions in the spectrum domain (kx,ky) is carried out instead of the complicated Fresnel integral. The approximations are obtained by the saddle-point integration. Comparison with experimental results demonstrates the validity of the approach.

Investigation on electromagnetic scattering from rough soil surface of layered medium using the small perturbation method

Electromagnetic scattering from a rough surface of layered medium is investigated, and the formulae of the scattering coefficients for different polarizations are derived using the small perturbation method. A rough surface with exponential correlation function is presented for describing a rough soil surface of layered medium, the formula of its scattering coefficient is derived by considering the spectrum of the rough surface with exponential correlation function; the curves of the bistatic scattering coefficient of HH polarization with variation of the scattering angle are obtained by numerical calculation. The influence of the permittivity of layered medium, the mean layer thickness of intermediate medium, the roughness surface parameters and the frequency of the incident wave on the blstatic scattering coefficient is discussed. Numerical results show that the influence of the permittivity of layered medium, the mean layer thickness of intermediate medium, the rms and the correlation length of the rough surface, and the frequency of the incident wave on the bistatic scattering coefficient is very complex.

A Mixed Convection Boundary Layer Flow over a Vertical Wall in a Porous Medium, with Exponentially Varying Fluid Viscosity

This study investigates a mixed convection boundary layer flow over a vertical wall embedded in a highly porous medium. The fluid viscosity is assumed to decrease exponentially with temperature. The boundary layer equations are transformed into a non-similar form using an appropriate non-similar variable ξ and a pseudo-similar variable η. The non-similar equations are solved using an efficient local non-similarity method. The effect of viscosity variation parameter on the heat transfer, skin friction and the velocity and temperature distribution within the boundary layer is investigated. The viscosity variation parameter, the viscous dissipation parameter and non-simi-larity variable are shown to have a significant effect on velocity and thermal boundary layer and also on the skin friction coefficient and heat transfer at the wall.

Similarity Solutions of Unsteady Convective Boundary Layer Flow along Isothermal Vertical Plate with Porous Medium

Similarity solution of unsteady convective boundary layer flow along isothermal vertical plate with porous medium is analyzed. The plate surface is reactive with the fluid and generates inert specie which diffuses inside the boundary. The flux of the specie at the plate is proportional to specie concentration at the plate. The governing equations of continuity, momentum, energy and specie diffusion are transformed into ordinary differential equation by using the similarity transformation and solved numerically by using free parameter method along with shooting technique. The dimensionless velocity, temperature and concentration profiles are obtained and presented through figures for different parameters entering into the problem. The local Skin-friction co-efficient, Nusselt number and Sherwood number at the plate for physical interest are also discussed through tables.

Effects of Solid Matrix and Porosity of Porous Medium on Heat Transfer of Marangoni Boundary Layer Flow Saturated with Power-Law Nanofluids

The effect of the solid matrix and porosity of the porous medium are first introduced to the study of power-law nanofluids, and the Marangoni boundary layer flow with heat generation is investigated. Two cases of solid matrix of porous medium including glass balls and aluminum foam are considered. The governing partial differential equations are simplified by dimensionless variables and similarity transformations, and are solved numerically by using a shooting method with the fourth-fifth-order Runge-Kutta integration technique. It is indicated that the increase of the porosity leads to the enhancement of heat transfer in the surface of the Marangoni boundary layer flow.

Green's function of multi-layered poroelastic half-space for models of ground vibration due to railway traffic

This study proposes a Green＇s function, an essential representation of water-saturated ground under moving excitation, to simulate ground borne vibration from trains. First, general solutions to the governing equations of poroelastic medium are derived by means of integral transform. Secondly, the transmission and reflection matrix approach is used to formulate the relationship between displacement and stress of the stratified ground, which results in the matrix of the Green＇s function. Then the Green＇s function is combined into a train-track-ground model, and is verified by typical examples and a field test. Additional simulations show that the computed ground vibration attenuates faster in the immediate vicinity of the track than in the surrounding area. The wavelength of wheel-rail unevenness has a notable effect on computed displacement and pore pressure. The variation of vibration intensity with the depth of ground is significantly influenced by the layering of the strata soil. When the train speed is equal to the velocity of the Rayleigh wave, the Mach cone appears in the simulated wave field. The proposed Green＇s function is an appropriate representation for a layered ground with shallow ground water table, and will be helpful to understand the dynamic responses of the ground to complicated moving excitation.

Analytical solutions for dynamic pressures of coupling fluid-solid-porous medium due to P wave incidence

Wave reflection and refraction in layered media is a topic closely related to seismology,acoustics,geophysics and earthquake engineering.Analytical solutions for wave reflection and refraction coefficients in multi-layered media subjected to P wave incidence from the elastic half-space are derived in terms of displacement potentials.The system is composed of ideal fluid,porous medium,and underlying elastic solid.By numerical examples,the effects of porous medium and the incident wave angle on the dynamic pressures of ideal fluid are analyzed.The results show that the existence of the porous medium,especially in the partially saturated case,may significantly affect the dynamic pressures of the overlying fluid.

Fretting Wear Behavior of Medium Carbon Steel Modified by Low Temperature Gas Multi-component Thermo-chemical Treatment

The introduction of surface engineering is expected to be an effective strategy against fretting damage. A large number of studies show that the low gas multi-component （such as carbon, nitrogen, sulphur and oxygen, etc） thermo-chemical treatment（LTGMTT） can overcome the brittleness of nitriding process, and upgrade the surface hardness and improve the wear resistance and fatigue properties of the work-pieces significantly. However, there are few reports on the anti-fretting properties of the LTGMTT modified layer up to now, which limits the applications of fretting. So this paper discusses the fretting wear behavior of modified layer on the surface of LZ50 （0.48%C） steel prepared by low temperature gas multi-component thermo-chemical treatment （LTGMTT） technology. The fretting wear tests of the modified layer flat specimens and its substrate （LZ50 steel） against 52100 steel balls with diameter of 40 mm are carried out under normal load of 150 N and displacement amplitudes varied from 2 μm to 40 μm. Characterization of the modified layer and dynamic analyses in combination with microscopic examinations were performed through the means of scanning electron microscope（SEM）, optical microscope（OM）, X-ray diffraction（XRD） and surface profilometer. The experimental results showed that the modified layer with a total thickness of 60 μm was consisted of three parts, i.e., loose layer, compound layer and diffusion layer. Compared with the substrate, the range of the mixed fretting regime（MFR） of the LTGMTT modified layer diminished, and the slip regime（SR） of the modified layer shifted to the direction of smaller displacement amplitude. The coefficient of friction（COF） of the modified layer was lower than that of the substrate in the initial stage. For the modified layer, the damage in partial slip regime（PSR） was very slight. The fretting wear mechanism of the modified layer both in MFR and SR was abrasive wear and delamination. The modified layer presented better wear resistance than the substrate in PSR and MFR; however, in SR, the wear resistance of the modified layer decreased with the increase of the displacement amplitudes. The experimental results can provide some experimental bases for promoting industrial application of LTGMTT modified layer in anti-fretting wear.

Superposition model for analyzing the dynamic ground subsidence in mining area of thick loose layer

The dynamic ground subsidence due to underground mining is a complicated time-dependent and rate- dependent process. Based. on the theory of rock rheology and probability integral method, this study developed the superposltlOn model for the prediction and analysis of the ground dynamic subsidence in mining area of thick !oose layer. The model consists of two parts （the prediction of overlying bedrock and the prediction of thick loose layer）. The overlying bedrock is regarded as visco-elastic beam, of which the dynamic subsidence is predicted by the Kelvin visco-elastic rheological model. The thick loose layer is regarded as random medium, and the ground dynamic subsidence, is predicted by the probability integral model. At last, the two prediction models are vertically stacked in the same coordinate system, and the bedrock dynamic subsidence is regarded as a variable mining thickness input into the prediction model of ground dynamic subsidence. The prediction results obtained were compared w^th actual movement and deformation data from Zhao I and Zhao II mine, central China. The agreement of the prediction results with the. field measurements.show that the superposition model （SM） is more satisfactory and the formulae obtained are more effective than the classical single probability Integral model（SPIM）, and thus can be effectively used for predicting the ground dynamic subsidence in mining area of thick loose layer.

Model and Emulation of the System for the Passive Electrostatic Detecting People Through the Medium

The plane medium and point charge are regarded as an integral system. The static field＇s distribution situation of the point charge is studied in the medium structure of plane division layers by using mirror image method, drawing the point charge＇s calculation expression of the electric potential in every medium space. The mathematics model of passive electrostatic detecting people through the medium （regular distance between the goal point charge and the medium） is established, using MATLAB software to carry out emulation of the model and analysing the result, establishing the theoretical foundation for studying the technology of the passive static detecting people through the wall.

Corrosion Mechanism of Alumina-magnesia Dry Materials for Smelting Manganese/chromium Steel in Coreless Medium Frequency Induction Furnaces

Alumina-magnesia dry materials are widely used in induction furnace linings, but they show different kinds of damage when melting different kinds of alloy steel. In this paper, the chemical composition, phase composition, and microstructure of the post-use dry materials for the working liners melting different kinds of steel were evaluated. Furthermore, the corrosion mechanism of the steel on the furnace lining materials was comprehensively analyzed. The findings reveal a significant ability of the Mn element in the molten steel to diffuse and penetrate into the refractories. Mn oxidizes to form MnO at the steel-refractory interface, and then forms a liquid phase with Al_(2)O_(3). The Cr element is dissolved into corundum and spinel of the refractories, resulting in lattice defects and structural damage of the materials. TiO2reacts with Al_(2)O_(3) to form Al_(2)TiO_(5), which plays a crucial role in preventing crack formation and propagation. Part of Ti4+dissolves into magnesia-alumina(MA), densifying the materials. TiO2also slows down the reaction between the Cr element and refractory components, further improving the corrosion resistance of the materials.

In-plane dynamic Green's functions for inclined and uniformly distributed loads in a multi-layered transversely isotropic half-space

The dynamic stiffness method combined with the Fourier transform is utilized to derive the in-plane Green’s functions for inclined and uniformly distributed loads in a multi-layered transversely isotropic（TI）half-space.The loaded layer is fixed to obtain solutions restricted in it and the corresponding reactions forces,which are then applied to the total system with the opposite sign.By adding solutions restricted in the loaded layer to solutions from the reaction forces,the global solutions in the wavenumber domain are obtained,and the dynamic Green’s functions in the space domain are recovered by the inverse Fourier transform.The presented formulations can be reduced to the isotropic case developed by Wolf（1985）,and are further verified by comparisons with existing solutions in a uniform isotropic as well as a layered TI halfspace subjected to horizontally distributed loads which are special cases of the more general problem addressed.The deduced Green’s functions,in conjunction with boundary element methods,will lead to significant advances in the investigation of a variety of wave scattering,wave radiation and soil-structure interaction problems in a layered TI site.Selected numerical results are given to investigate the influence of material anisotropy,frequency of excitation,inclination angle and layered on the responses of displacement and stress,and some conclusions are drawn.

Unsteady hydromagnetic Couette flow through a porous medium in a rotating system

This paper investigates the unsteady hydromagnetic Couette fluid flow through a porous medium between two infinite horizontal plates induced by the non-torsional oscillations of one of the plates in a rotating system using boundary layer approximation.The fluid is assumed to be Newtonian and incompressible.Laplace transform technique is adopted to obtain a unified solution of the velocity fields.Such a flow model is of great interest,not only for its theoretical significance,but also for its wide applications to geophysics and engineering.Analytical expressions for the steady state velocity and shear stress on the plates are obtained,and the case of single oscillating plate is also discussed.The influence of pertinent parameters on the flow is delineated,and appropriate conclusions are drawn.

RECONSTRUCTION OF ONE DIMENSIONAL MULTI-LAYERED MEDIA BY USING A TIME DOMAIN SIGNAL FLOW GRAPH TECHNIQUE

A novel inverse scattering method to reconstruct the permittivity profile of one-dimensional multi-layered media is proposed in this paper.Based on the equivalent network ofthe medium,a concept of time domain signal flow graph and its basic principles are introduced,from which the reflection coefficient of the medium in time domain can be shown to be a series ofDirac δ-functions(pulse responses).In terms of the pulse responses,we will reconstruct both thepermittivity and the thickness of each layer will accurately be reconstructed.Numerical examplesverify the applicability of this

Unsteady Couette Flow through a Porous Medium in a Rotating System

An investigation has been made on an unsteady Couette flow of a viscous incompressible fluid through a porous me- dium in a rotating system. The solution of the governing equations has been obtained by the use of Laplace transform technique. It is found that the primary velocity decreases and the magnitude of the secondary velocity increases with an increase in rotation parameter. The fluid velocity components are decelerated by an increase of Reynolds number. An increase in porosity parameter leads to increase the primary velocity and the magnitude of the secondary velocity. It is also found that the solution for small time converges more rapidly than the general solution. The asymptotic behavior of the solution is analyzed for small as well as large values of rotation parameter and Reynolds number. It is observed that a thin boundary layer is formed near the moving plate of the channel and the thicknesses of the boundary layer increases with an increase in porosity parameter.

Link Layer Correction Techniques and Impact on TCP’s Performance in IEEE 802.11 Wireless Networks

TCP performance degrades when end-to-end connections extend over wireless links which are characterized by high Bit Error Rate and intermittent connectivity. Such degradation is mainly accounted for TCP’s unnecessary congestion control actions while attempting TCP loss recovery. Several independent link loss recovery approaches are proposed by researchers to reduce number of losses visible at TCP. In this paper we first presented a survey of loss mitigation techniques at wireless link layer. Secondly performance evaluation for TCP through Type 0 Automatic Retransmission Request mechanism in erroneous Wireless LAN is presented. In particular, simulations are performed taking into account the wireless errors introduced over IEEE 802.11 link using a well-established 2-State Markov model. TCP performance is evaluated under different settings for maximum link retransmissions allowed for each frame. Simulation results show that, link retransmission improves TCP performance by reducing losses perceived at TCP sender. However, such improvement is often associated with adverse effect on other TCP parameters that may cost a lot in return under extreme network conditions. In this paper an attempt is made to observe impact of link retransmissions on the performance of multiple TCP flows competing with each other. The analysis presented in this paper signifies the scope for maximizing TCP’s throughput at the least possible cost.