Infrared neuron stimulation is regarded as an innovative approach for stimulating cochleae in animals while the exact mechanism still remains unknown.In this paper,we studied compound action potentials of guinea pig c...Infrared neuron stimulation is regarded as an innovative approach for stimulating cochleae in animals while the exact mechanism still remains unknown.In this paper,we studied compound action potentials of guinea pig cochleae with chronic or acute deafness.We recorded optical compound action potentials and analyzed stretched cochlear preparations by fiuorescence microscopy.Photoacoustic signals were measured by hydrophone and microphone,respectively.In our experiment,we observed a switch response effect in vitro and in vivo experiments.Therefore,we proposed photoacoustic effect could invoke auditory response in infrared neuron stimulation.展开更多
For the first time, we report photoacoustic (PA) signal detection in a cell placed within the Michelson interferometer cavity in an attempt to relate photoacoustic effect to the Michelson fringe shift as a result of c...For the first time, we report photoacoustic (PA) signal detection in a cell placed within the Michelson interferometer cavity in an attempt to relate photoacoustic effect to the Michelson fringe shift as a result of changes in the cell. Both detection schemes were investigated using IR absorption and their sensitivities compared. Signals related to Michelson interferometer fringe and PA effect have shown good correlations with each other using different samples including some essential oils and their corresponding plant part from which the essential oil is usually obtained. Results were encouraging and will open the door widely to use the combined Michelson interferometer-photoacoustic spectroscopy (PAS) in trace gas detection for different applications.展开更多
Rapid and cost effective quantification of lignocellulosic components （cellulose, hemicelluloses and lignin） of agricultural biomass （barley, canola, oat and wheat） is essential to determine the effect of various ...Rapid and cost effective quantification of lignocellulosic components （cellulose, hemicelluloses and lignin） of agricultural biomass （barley, canola, oat and wheat） is essential to determine the effect of various pre-treatments （such as steam explosion） on biomass used as feedstock for the biofuel industry. Fourier Transformed Infrared （FTIR） spectroscopy was considered as an option to achieve this objective. Regression equations having R2 values of 0.89, 0.99 and 0.98 were developed to predict the cellulose, hemicelluloses and lignin compounds of biomass, respectively. The average absolute difference in predicted and measured cellulose, hemicellulose and lignin in agricultural biomass was 7.5%, 2.5%, and 3.8%, respectively.展开更多
In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting &a...In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting αvβ3 and αvβ5 integrins was investigated either via IV or locoregional injections. We additionally documented the potential interferences of this Near Infrared Fluorescence Probe with Blue Patente V and ICG dyes routinely used to facilitate lymph node detection during surgery. The human mammary adenocarcinoma MDA-MB-231-luc model was injected into the forepaw of nude female rats to obtain a controlled invasion of the axillary LN. Thanks to its high sensitivity, BLI was selected to achieve in vivo quantitation of tumor cells in SLNs and determine eligible animals for the study. NIRF of integrins was performed at 680 nm both in vivo and ex vivo using spectral unmixing to suppress auto-fluorescence signal and preserve sensitivity. In vivo BLI was quite reliable in estimating discrete invasion by cancer cells in the LN with thresholds of detection and quantitation of about 500 and 1500 cells respectively. For fluorescence at 680 nm, in vivo imaging is not suitable to detect micro-invasion, but ex vivo fluorescence with spectral unmixing of SLNs confirmed the presence of a tumor burden as low as 1500 cells expressing αvβ3/αvβ5 integrins. Targeting few tumor cells inside a micro-invaded sentinel lymph node by molecular probes is not sensitive enough to provide direct in vivo or peroperative imaging. At the time NIRF is performed on the excised specimen, high sensitivity imaging associated with spectral unmixing allowed such detection within less than 1 minute of examination.展开更多
Photoacoustic waves from hemoglobin solutions in dental roots are detected by using a 1064-nm laser and an ultrasonic soft probe based on a composite transducer on the tooth surface. The high-frequency ultrasonic wave...Photoacoustic waves from hemoglobin solutions in dental roots are detected by using a 1064-nm laser and an ultrasonic soft probe based on a composite transducer on the tooth surface. The high-frequency ultrasonic waves are detected from a tooth with a hemoglobin solution in the pulp cavity due to the large heat transfer coefficient and absorption coefficient of hemoglobin. The spectral intensities of frequency components higher than 1 MHz show good correlation with the hemoglobin solution concentrations, and maps of frequency spectra calculated by taking short-time Fourier transforms clearly exhibit the effect of absorbance in dental pulp.展开更多
基金This projectwas supported by grants fromtheNature Science Foundation of China(Nos.NSFC81401539 and NSFC31271056)and the projects in the Shenzhen Medical Engineering Laboratory For Human Auditory-equilibrium Function.
文摘Infrared neuron stimulation is regarded as an innovative approach for stimulating cochleae in animals while the exact mechanism still remains unknown.In this paper,we studied compound action potentials of guinea pig cochleae with chronic or acute deafness.We recorded optical compound action potentials and analyzed stretched cochlear preparations by fiuorescence microscopy.Photoacoustic signals were measured by hydrophone and microphone,respectively.In our experiment,we observed a switch response effect in vitro and in vivo experiments.Therefore,we proposed photoacoustic effect could invoke auditory response in infrared neuron stimulation.
文摘For the first time, we report photoacoustic (PA) signal detection in a cell placed within the Michelson interferometer cavity in an attempt to relate photoacoustic effect to the Michelson fringe shift as a result of changes in the cell. Both detection schemes were investigated using IR absorption and their sensitivities compared. Signals related to Michelson interferometer fringe and PA effect have shown good correlations with each other using different samples including some essential oils and their corresponding plant part from which the essential oil is usually obtained. Results were encouraging and will open the door widely to use the combined Michelson interferometer-photoacoustic spectroscopy (PAS) in trace gas detection for different applications.
文摘Rapid and cost effective quantification of lignocellulosic components （cellulose, hemicelluloses and lignin） of agricultural biomass （barley, canola, oat and wheat） is essential to determine the effect of various pre-treatments （such as steam explosion） on biomass used as feedstock for the biofuel industry. Fourier Transformed Infrared （FTIR） spectroscopy was considered as an option to achieve this objective. Regression equations having R2 values of 0.89, 0.99 and 0.98 were developed to predict the cellulose, hemicelluloses and lignin compounds of biomass, respectively. The average absolute difference in predicted and measured cellulose, hemicellulose and lignin in agricultural biomass was 7.5%, 2.5%, and 3.8%, respectively.
文摘In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting αvβ3 and αvβ5 integrins was investigated either via IV or locoregional injections. We additionally documented the potential interferences of this Near Infrared Fluorescence Probe with Blue Patente V and ICG dyes routinely used to facilitate lymph node detection during surgery. The human mammary adenocarcinoma MDA-MB-231-luc model was injected into the forepaw of nude female rats to obtain a controlled invasion of the axillary LN. Thanks to its high sensitivity, BLI was selected to achieve in vivo quantitation of tumor cells in SLNs and determine eligible animals for the study. NIRF of integrins was performed at 680 nm both in vivo and ex vivo using spectral unmixing to suppress auto-fluorescence signal and preserve sensitivity. In vivo BLI was quite reliable in estimating discrete invasion by cancer cells in the LN with thresholds of detection and quantitation of about 500 and 1500 cells respectively. For fluorescence at 680 nm, in vivo imaging is not suitable to detect micro-invasion, but ex vivo fluorescence with spectral unmixing of SLNs confirmed the presence of a tumor burden as low as 1500 cells expressing αvβ3/αvβ5 integrins. Targeting few tumor cells inside a micro-invaded sentinel lymph node by molecular probes is not sensitive enough to provide direct in vivo or peroperative imaging. At the time NIRF is performed on the excised specimen, high sensitivity imaging associated with spectral unmixing allowed such detection within less than 1 minute of examination.
文摘Photoacoustic waves from hemoglobin solutions in dental roots are detected by using a 1064-nm laser and an ultrasonic soft probe based on a composite transducer on the tooth surface. The high-frequency ultrasonic waves are detected from a tooth with a hemoglobin solution in the pulp cavity due to the large heat transfer coefficient and absorption coefficient of hemoglobin. The spectral intensities of frequency components higher than 1 MHz show good correlation with the hemoglobin solution concentrations, and maps of frequency spectra calculated by taking short-time Fourier transforms clearly exhibit the effect of absorbance in dental pulp.
