Sequence Analysis on Interleukin-2 Gene and Interleukin-6 Gene in a Hybrid Pig

[ Objective] To understand the functions of interteukin-2 （IL-2） and interleukin-6 （IL-6） in immune response. [ Method] Peripheral lymphocytas were isolated from a hybrid pig （ Duroc x Landrace x Shandong local pig） and stimulated with canavalin A in vitro. With total RNA as templates, the IL-2 and IL-6 cDNA were amplified by the RT-PCR and sequenced, respectively. [Result] The sequence of IL-2 gene had 100.0% nucleotide homology to the published IL-2 sequences. The sequence of IL-6 gene had 99.8% -100.0% nucleotide homology to the published IL-6 sequences, and the amino acid homology was 99.1% -100.0%. [ Conclusion] NO great variation of the IL-2 gene and IL-6 gene is observed in the hybdd pig, and these results provide a basis for research about functions of IL-2 and IL-6 protein.

The effect of interleukin-6 gene transfer on human cord blood megaka ryopoiesis

Objective:To investigate the effect of IL-6 gene transfe r into human cord blood hematopoietic stem cells on the production of megakaryocy t ic progenitors.Methods:IL-6 gene was transfected into human c o rd blood CD34+ cells using a retrovirus vector with the aid of recombinant fibro nectin fragments in the presence of a cocktail of cytokines (SCF,IL-6,sIL-6R,F L,and TPO).Colony-forming units-megakaryocyte (CFU-MK) assays were performed as IL-6 gene transduced CD34+ cells were incubated alone or in combination with I L -3 or sIL-6R, controlled with neoR gene transduced CD34+ cells.Result s :IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies,the addit ion of sIL-6R to IL-6 gene transduced CD34+ cells significantly enhanced the prod uction of CFU-MK colonies.IL-6 gene transduced CD34+ cells showed a modest syn er gistic effect with IL-3.Conclusion:These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytop en ia.

A rare missense PAX6 mutation causes atypical aniridia in a three-generation Chinese family

●AIM:To investigate the molecular diagnosis of a threegeneration Chinese family affected with aniridia,and further to identify clinically a PAX6 missense mutation in members with atypical aniridia.●METHODS:Eleven family members with and without atypical aniridia were recruited.All family members underwent comprehensive ophthalmic examinations.A combination of whole exome sequencing(WES)and direct Sanger sequencing were performed to uncover the causative mutation.●RESULTS:Among the 11 family members,8 were clinically diagnosed with congenital aniridia(atypical aniridia phenotype).A rare heterozygous mutation c.622C>T(p.Arg208Trp)in exon 8 of PAX6 was identified in all affected family members but not in the unaffected members or in healthy control subjects.●CONCLUSION:A rare missense mutation in the PAX6 gene is found in members of a three-generation Chinese family with congenital atypical aniridia.This result contributes to an increase in the phenotypic spectrum caused by PAX6 missense heterozygous variants and provides useful information for the clinical diagnosis of atypical aniridia,which may also contribute to genetic counselling and family planning.

Identification of marker genes associated with N6-methyladenosine and autophagy in ulcerative colitis

BACKGROUND Both N6-methyladenosine(m6A)methylation and autophagy are considered relevant to the pathogenesis of ulcerative colitis(UC).However,a systematic exploration of the role of the com-bination of m6A methylation and autophagy in UC remains to be performed.AIM To elucidate the autophagy-related genes of m6A with a diagnostic value for UC.METHODS The correlation between m6A-related genes and autophagy-related genes(ARGs)was analyzed.Finally,gene set enrichment analysis(GSEA)was performed on the characteristic genes.Additionally,the expression levels of four characteristic genes were verified in dextran sulfate sodium(DSS)-induced colitis in mice.RESULTS GSEA indicated that BAG3,P4HB and TP53INP2 were involved in the inflammatory response and TNF-αsignalling via nuclear factor kappa-B.Furthermore,polymerase chain reaction results showed significantly higher mRNA levels of BAG3 and P4HB and lower mRNA levels of FMR1 and TP53INP2 in the DSS group compared to the control group.CONCLUSION This study identified four m6A-ARGs that predict the occurrence of UC,thus providing a scientific reference for further studies on the pathogenesis of UC.

Effect of flurbiprofen combined with prednisolone on interleukin-6 in elderly surgery patients

Objective: To determine the effect of flurbiprofen combined with prednisolone on interleukin-6 in elderly surgery patients. Methods: In this double-blind randomized controlled study, patients aged 65 to 80 who were undergoing spinal fusion surgery for disc herniation were administered flurbiprofen 100 mg (P group, flurbiprofen group), prednisolone 0.6 mg/kg (D group, prednisolone group), prednisolone 0.6 mg/kg plus flurbiprofen 100 mg (P + D group, flurbiprofen + prednisolone group) or normal saline (S group, saline group) 15 minutes before the induction of anesthesia. Plasma samples were collected before surgery (T0) and on day 1 (T1), day 2 (T2) and day 3 (T3) following surgery. At the same time, systemic inflammatory response syndrome (SIRS) was assessed by SIRS criteria. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) for collected samples were measured. Results: Other groups had significantly lower levels of IL-6, CRP and occurrence of SIRS than S group (p < 0.05). Compared to groups P and D, the levels of IL-6 and CRP in P + D group were significantly lower on T1 (p < 0.05). Peak levels of IL-6 in all groups were presented on T1 (p 0.05). The levels of CRP within three days were significantly different but did not show peak levels (p > 0.05). Conclusion: Compared to prednisolone or flurbiprofen, combining flurbiprofen with prednisolone in elderly surgery patients led to an increased suppression of IL-6.

Analysis of the Role of D-Dimer,Interleukin-6,and Interleukin-18 in Differential Diagnosis of Pediatric Refractory Mycoplasma pneumoniae Pneumonia

Objective:To analyze the value of D-dimer(D-D),interleukin-6(IL-6),and IL-18 in the differential diagnosis of children with refractory Mycoplasma pneumoniae pneumonia(RMPP).Methods:The medical records of 92 children with Mycoplasma pneumoniae pneumonia(MPP)treated in the hospital were selected for retrospective analysis from January 2023 to January 2024.After comprehensive examinations such as computed tomography examination of the chest,48 children with general Mycoplasma pneumoniae pneumonia(GMPP)were put in the GMPP group and 44 children with RMPP were grouped in the RMPP group.The IL-6,IL-18,and D-D levels were compared between the two groups,and the receiver operating characteristic(ROC)curves were plotted to analyze their value for differential diagnosis of RMPP.Results:The levels of IL-6,IL-18,and D-D in the RMPP group were higher than those in the GMPP group(P<0.05);the ROC curves showed that the specificity of the differential diagnosis of IL-6,IL-18,and D-D was higher,and their diagnostic value was significant.Conclusion:Determination of IL-6,IL-18,and D-D levels in children with MPP can further diagnose the children’s condition,which can help physicians formulate targeted treatment plans,and is of great significance to the improvement of the children’s condition,which is worthy of attention.

Efficacy of Laparoscopic Cholecystectomy in Treating Patients with Gallstones and Its Effect on Interleukin-6 and Tumor Necrosis Factor-α Levels

Objective:To investigate the efficacy of laparoscopic cholecystectomy in the treatment of patients with gallstones and its effect on the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-a).Methods:A total of 82 patients with gallstones admitted from July 2020 to July 2023 were recruited and allocated into control and observation groups using the random number table method,with 41 cases in each group.The patients were treated with laparoscopic cholecystectomy,with the anterior triangle anatomical approach to the gallbladder in the control group and the posterior triangle anatomical approach to the gallbladder in the observation group.The treatment effect and inflammatory factor levels of both groups were observed and compared.Results:When comparing the clinical outcomes of both patient groups,the key parameters evaluated included time to mobilization,duration of surgery,extubation time,and intraoperative bleeding.The observation group exhibited a significant advantage in these parameters compared to the control group(P<0.05).Regarding the levels of inflammatory factors between the two groups before and after treatment,there was no significant difference in values before treatment.However,following treatment,patients in the observation group showed significantly lower levels of IL-6,TNF-α,and C-reactive protein(CRP)compared to the control group(P<0.05).Conclusion:Patients undergoing laparoscopic cholecystectomy for gallstones can benefit from the implementation of the posterior triangular anatomical approach to the gallbladder,which not only enhances therapeutic efficacy but also offers significant advantages in reducing levels of IL-6,TNF-α,and CRP.Therefore,it is recommended for the widespread adoption of this treatment approach in clinical practice.

白介素1β及白介素6基因单核苷酸多态性与食管癌放疗后肺部感染的关联

目的分析人白介素1β(interleukin 1β,IL-1β)、白介素6(interleukin 6,IL-6)基因单核苷酸多态性与食管癌放疗后肺部感染的关联,并探讨其生理病理的调控作用。方法选取新乡医学院第三附属医院2019年1月~2022年7月收治的115例食管癌患者为研究对象,根据放疗后是否出现感染分为感染组(n=28)和非感染组(n=87),检测IL-1β基因单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点-511C/T(rs16944)、-31C/T(rs1143627)和IL-6基因单核苷酸多态性位点IL-6-572C/G(rs1800796)、IL-6-174G/C(rs1800795)的多态性,采用Spearman相关检验分析IL-1β、IL-6基因单核苷酸多态性与食管癌放疗后肺部感染的关系,多因素Logistic回归分析影响食管癌放疗后肺部感染的相关因素。结果两组患者性别、年龄、BMI、临床分期等基线资料比较,差异无统计学意义(P>0.05);放疗时间、肿瘤长度、肿瘤位置比较差异有统计学意义(P<0.05);IL-1β基因的rs16944、rs1143627位点,IL-6基因的rs1800796、rs1800795位点的基因型分布均符合Hardy-Weinberg遗传平衡定律(P>0.05);两组IL-1β基因rs16944位点基因型频率,CC、CT、TT基因型频率,C、T等位基因频率,差异均无统计学意义(P>0.05);rs1143627位点CC、CT、TT基因型频率,C、T等位基因频率差异均有统计学意义(P<0.05);IL-6基因rs1800796位点CC、CG、GG基因型频率,C、G等位基因频率差异均有统计学意义(P<0.05),rs1800795位点CC、CG、GG基因型频率,C、G等位基因频率差异均无统计学意义(P>0.05);Spearman相关检验分析显示,IL-1βrs1143627(C/T)位点、IL-6 rs1800796(C/T)位点基因多态性与食管癌放疗后肺部感染具有显著相关性(P<0.05);多因素Logistic回归分析显示,放疗时间、肿瘤长度、肿瘤位置、IL-1βrs1143627位点、IL-6 rs1800796位点单核苷酸多态性是影响食管癌患者放疗后肺部感染的多因素(P<0.05)。结论IL-1β基因rs1143627(C/T)位点多态性及IL-6基因rs1800796(C/G)位点多态性与食管癌放疗后肺部感染有关,其中rs1143627位点T等位基因、rs1800796位点G等位基因可能是易感基因。

老年舒张性心力衰竭合并肌少症患者可溶性生长刺激表达基因2蛋白、肌红蛋白、白细胞介素-6水平与心功能的相关性

目的 探讨老年舒张性心力衰竭(DHF)合并肌少症患者外周血可溶性生长刺激表达基因2蛋白(sST2)、肌红蛋白(Myo)、白细胞介素-6(IL-6)水平与心功能的相关性。方法 将122例DHF患者根据有无肌少症分为DHF合并肌少症组60例和DHF组62例,另将健康体检者58例、单纯肌少症患者60例分别纳入对照组、单纯肌少症组,检测各组外周血sST2、Myo、IL-6水平和心功能指标[左室射血分数(LVEF)、心排血量(CO)、心率(HR)、每搏输出量(SV)和心脏指数(CI)]。采用Pearson相关分析法分析sST2、Myo、IL-6与各心功能指标的相关性。绘制受试者工作特征(ROC)曲线,分析sST2、Myo、IL-6单独及联合诊断DHF合并肌少症的效能。结果 与对照组、单纯肌少症组相比,DHF组、DHF合并肌少症组sST2、Myo、IL-6水平和HR均升高,LVEF、CO、SV和CI均降低,差异有统计学意义(P<0.05);与DHF组相比,DHF合并肌少症组sST2、Myo、IL-6水平和HR均升高,LVEF、CO、SV和CI均降低,差异有统计学意义(P<0.05)。sST2、Myo、IL-6均分别与LVEF、CO、SV、CI呈负相关(P<0.001),均与HR呈正相关(P<0.001);sST2、Myo、IL-6、LVEF、SV是DHF合并肌少症的独立影响因素(P<0.05);sST2、Myo、IL-6联合诊断DHF合并肌少症的曲线下面积为0.936,诊断效能优于三者单独检测。结论 老年DHF合并肌少症患者外周血sST2、Myo、IL-6水平显著升高,且sST2、Myo、IL-6均与心功能指标显著相关,三者联合检测对DHF合并肌少症的诊断效能较高。

贵州地区急性淋巴细胞白血病儿童TPMT、NUDT15基因多态性与6-MP耐受性分析

目的观察贵州地区ALL儿童TPMT、NUDT15基因多态性,探讨其与6-MP耐受性的关系。方法收集贵阳市儿童医院血液科住院的贵州地区ALL儿童。Sanger法检测患者NUDT15c.415C>T和TPMT*2、TPMT*3A、TPMT*3B、TPMT*3C基因型。所有ALL儿童均按CCLG-2008方案化疗,每周1次监测血常规及肝肾功能。根据2016新编WHO化疗药物毒性反应分度标准,出现Ⅲ~Ⅳ度与6-MP相关的毒性反应,称为6-MP不耐受(除外感染、其他药物影响)。分析TPMT、NUDT15基因多态性与6-MP不耐受的相关性。结果共纳入患者60例,检测到TPMT突变(中间代谢型)3例(5%),正常代谢型57例(95%)。NUDT15基因CT型12例(20%),TT型1例(1.7%),CC型(野生型)47例(78.3%)。NUDT15基因突变率21.7%(13/60)显著高于TPMT基因突变率5%(3/60),差异有统计学意义(P=0.007)。TPMT突变型3例均发生6-MP不耐受(100%),NUDT15突变型13例中11例发生6-MP不耐受(84.6%)。结论TPMT、NUDT15基因突变与6-MP不耐受相关(P<0.05),联合检测TPMT、NUDT15基因对调整6-MP使用,减少6-MP不良反应提供依据。

Genetic association of interleukin-6 polymorphism (-174 G/C) with chronic liver diseases and hepatocellular carcinoma

Interleukin-6 (IL-6) is a pleiotropic cytokine which is expressed in many inflammatory cells in response to different types of stimuli, regulating a number of biological processes. The IL-6 gene is polymorphic in both the 5' and 3' flanking regions and more than 150 single nucleotide polymorphisms have been identified so far. Genetic polymorphisms of IL-6 may affect the outcomes of several diseases, where the presence of high levels of circulating IL-6 have been correlated to the stage and/or the progression of the disease itself. The -174 G/C polymorphism is a frequent polymorphism, that is located in the upstream regulatory region of the IL-6 gene and affects IL-6 production. However, the data in the literature on the genetic association between the -174 G/C polymorphism and some specific liver diseases characterized by different etiologies are still controversial. In particular, most of the studies are quite unanimous in describing a correlation between the presence of the high-producer genotype and a worse evolution of the chronic liver disease. This is valid for patients with hepatitis C virus (HCV)-related chronic hepatitis and liver cirrhosis and hepatocellu-lar carcinoma (HCC) whatever the etiology. Studies in hepatitis B virus-related chronic liver diseases are not conclusive, while specific populations like non alcoholic fatty liver disease/non-alcoholic steatohepatitis, autoimmune and human immunodeficiency virus/HCV coinfected patients show a higher prevalence of the lowproducer genotype, probably due to the complexity of these clinical pictures. In this direction, a systematic revision of these data should shed more light on the role of this polymorphism in chronic liver diseases and HCC.

MEK AND p38 MAPK-DEPENDANT PATHWAYS ARE INVOLOVED IN THE POSITIVE EFFECT OF INTERLEUKIN-6 ON HUMAN GROWTH HORMONE GENE EXPRESSION IN RAT MtT/S SOMATOTROPH CELLS

Objective To investigate the effect of interleukin-6(IL-6)on the human growth hormone(hGH)gene expression in a rat somatotropic pituitary cell line MtT/S.Methods The plasmids containing various lengths of hGH gene 5'-promoter fragments were constructed.Stably transfected MtT/S cells were created by cotransfecting the above plasmids and pcDNA3.1(+)with DMRIE-C transfection reagent.After the administration of these cells with IL-6 and/or various inhibitors of signaling transduction pathways,the luciferase activities in MtT/S cells lysis were assayed to demonstrate the effects of IL-6 on hGH gene promoter activity and possibly involved mechanism.Results The 103 U/mL IL-6 stimulated GH secretion and synthesis,and promoted the 5'-promoter activity of GH gene in stably transfected MtT/SGL cells with the action of 1.69 times above the control.Among inhibitors of signaling transduction pathways,mitogen-activated protein kinase kinase(MAPKK/MEK)inhibitor PD98059(40 μmol/L)and p38 mitogen-activated protein kinase(MAPK)inhibitor SB203580(5 μmol/L)completely blocked the stimulatory effect of IL-6.Western blot analysis further confirmed the activation of phosphorylated MEK and p38 MAPK in MtT/SGL cells.Neither over-expression of Pit-1 nor inhibition of Pit-1 expression affected IL-6 induction of hGH promoter activity.A series of deletion constructs of hGH promoter were created to identify the DNA sequence that mediated the effect of IL-6.The results showed that the stimulatory effect of IL-6 was abolished following deletion of the-196 to-132 bp fragment.Conclusions IL-6 promotes GH secretion and synthesis by rat MtT/S somatotroph cells.The stimulatory effect of IL-6 on hGH gene promoter appears to require the activation of MEK and p38 MAPK,and a fragment of promoter sequence that spans the-196 to-132 bp of the gene,but may be unlinked with Pit-1 protein.

急性淋巴细胞白血病患儿维持治疗期间6-巯基嘌呤使用剂量与TPMT和NUDT15基因多态性的关系

背景6-巯基嘌呤(6-MP)是急性淋巴细胞白血病(ALL)维持治疗最主要的药物之一,代谢酶基因多态性与药物的骨髓抑制程度有关。目的探讨ALL患儿维持治疗期间6-MP使用剂量与TPMT和NUDT15基因多态性的关系。设计回顾性队列研究。方法纳入2020年1月至2021年12月于郑州大学第一附属医院明确诊断为ALL、经前期化疗骨髓达完全缓解、进入维持治疗阶段且口服6-MP治疗的患儿。口服6-MP前患儿行TPMT和NUDT15基因型检测,分析TPMT、NUDT15不同基因型患儿应用6-MP治疗的使用剂量和骨髓抑制情况。主要结局指标6-MP使用剂量。结果61例维持治疗的ALL患儿纳入本文分析。纳入TPMT基因多态性统计分析的ALL患儿48例(除外NUDT15基因突变13例),野生型45例,突变型3例;纳入NUDT15基因多态性统计分析的ALL患儿58例(除外TPMT基因突变3例),CC型45例,CT型9例,TT型4例。在6-MP维持治疗阶段,TPMT和NUDT15不同基因型患儿的WBC、Hb、PLT计数差异均有统计学意义(均P<0.05)。患儿用药后出现不同程度的中性粒细胞减少,包括TPMT野生型15例,突变型3例;NUDT15基因CC型15例,CT型5例,TT型4例;TPMT和NUDT15不同基因型患儿骨髓抑制情况差异均有统计学意义(均P<0.05)。TPMT和NUDT15不同基因型患儿6-MP使用剂量差异有统计学意义(均P<0.05),TPMT野生型和突变型ALL患儿维持治疗期6-MP使用剂量分别为(40.81±6.02)和(16.25±4.42)mg·m^(-2)·d^(-1);NUDT15基因CC型、CT型和TT型6-MP使用剂量分别为(40.81±6.02)、(34.28±4.53)和(10.00±1.28)mg·m^(-2)·d^(-1)。结论TPMT和NUDT15突变型患儿的骨髓抑制程度均较野生型严重。ALL患儿维持治疗期间6-MP使用剂量与TPMT和NUDT15基因多态性相关。

鸡FGF6基因生物学特性及其多态性与经济性状的关联分析

旨在探究鸡成纤维细胞生长因子6(fibroblast growth factor 6,FGF6)基因的生物学特性,挖掘FGF6基因单核苷酸多态性(SNP)位点并分析其与经济性状的相关性,为后续研究鸡FGF6基因对生长性状的影响提供参考。本研究对FGF6蛋白序列进行生物信息学分析;基于768只固始鸡-安卡鸡F_2资源群的GBS数据及相关经济性状表型数据对FGF6基因包含启动子2 kb区域SNP位点进行筛选,并进行连锁不平衡和关联分析,探究FGF6基因SNP和单倍型与鸡经济性状的相关性。生物信息学分析结果显示,鸡FGF6氨基酸序列与火鸡同源性为91.26%;系统进化分析表明,鸡FGF6与火鸡亲缘关系最近,其次是绿海龟,与斑马鱼遗传距离最远;保守性分析显示不同物种间FGF6保守性较高。鸡FGF6蛋白为亲水性蛋白、存在1个跨膜区,主要定位于细胞质;其二级、三级结构相符。鸡FGF6蛋白被22个磷酸化位点和1个N-糖基化位点修饰;与其受体家族FGFR1~4和EGF存在互作关系。鸡FGF6基因启动子区域筛选到6个SNPs位点,均位于1号内含子上;多态性分析显示6个SNPs位点存在高多态性,其中5个处于哈代-温伯格平衡,呈强连锁状态(D′=1,r^(2)=1)。关联分析结果显示,rs73399071位点与F_2资源群不同阶段体重(BW)、胫长(SL)、胫围(SG)、体斜长(BSL)等17个生长性状指标显著相关(P<0.05),与全净膛重(EW)、半净膛重(SEW)、屠体重(CW)、胸肌重(BMW)等10个屠体性状指标显著相关(P<0.05),与肉质性状的关联未达到显著水平(P>0.05),与高密度脂蛋白(HDL)和胆碱酯酶(ChE)显著相关(P<0.05),CC基因型个体的大部分性状表型均值高于GG基因型个体;单倍型组合分析显示不同阶段CCTTCCTTCC组合个体的体重均高于其他单倍型组合个体。本研究结果为进一步探究鸡FGF6基因的功能提供理论参考,筛选到FGF6基因存在5个与固始鸡-安卡鸡F_2资源群体重、体尺、屠体性状和血液生化指标显著关联的SNPs位点,为鸡育种提供候选的分子标记,为地方鸡标记辅助选择提供新的思路。

6-Gingerol, asarinin, and deoxyschizandrin improve bronchial epithelium functions in an interleukin-13einduced BEAS-2B cell model

Objective:To explore the effects of 6-gingerol,asarinin,and deoxyschizandrindthe main components of Zingiber officinale(Willd.)Rosc.(Gan Jiang),Asarum heterotropoides f.var.mandshuricum(Maxim.)(Xi Xin),and Schisandra chinensis(Turcz.)Baill.(Wu Wei Zi),respectivelydon an interleukin(IL)-13einduced BEAS-2B cell model in vitro.Methods:The BEAS-2B cell model was established using 25 ng/mL IL-13 combined with 1%fetal bovine serum(FBS)in vitro.Mitoquinone mesylate(Mito-Q)treatment was used as a positive control group,and different concentrations of 6-gingerol,asarinin,and deoxyschizandrin were used to treat the models.The level of reactive oxygen species(ROS)production was detected by flow cytometry.The expression levels of LC3B,Beclin1,adenosine 50-monophosphate(AMP)eactivated protein kinase(AMPK),phosphory-lated-AMPeactivated protein kinase(P-AMPK),dynamin-related protein 1(DRP1),and mitochondrial fusion protein 2(MFN2)were detected by Western blot.Mitochondrial membrane potential(MMP)assay kit with JC-1 was utilized to detect the level of MMP.Results:The BEAS-2B cells exposed to 25 ng/mL IL-13 with 1%FBS showed an increased ROS level and a decreased MMP.6-Gingerol,asarinin,and deoxyschizandrin were able to downregulate ROS level and upregulate the MMP in the BEAS-2B model.Asarinin and deoxyschizandrin reduced the expression of autophagy protein LC3B,while deoxyschizandrin significantly increased the expression of DRP1 in the BEAS-2B model.Conclusion:6-Gingerol,asarinin,and deoxyschizandrin can reduce ROS generation and increase MMP,but have different regulatory effects on the expression of autophagy protein and mitochondrial mitotic protein.The three components have both synergistic and complementary effects in classic medicine compatibility.This study may provide an innovative strategy to reduce the lung inflammation related to IL-13.

杨树桑黄6-磷酸海藻糖合成酶基因克隆鉴定与表达分析

对杨树桑黄(Sanghuangporus vaninii)6-磷酸海藻糖合成酶基因(SvTPS)进行克隆和生物信息学分析,并进行原核表达;采用荧光定量PCR方法研究SvTPS在杨树桑黄菌丝体和不同年份子实体中的表达情况,测定菌丝体和不同年份子实体中SvTPS活性。结果表明:SvTPS DNA序列长度为1894 bp,编码576个氨基酸。SvTPS相对分子质量为65130,等电点为6.21,脂肪系数为88.14,平均亲水系数为-0.281,不稳定系数为42.41。SvTPS氨基酸序列与暴马桑黄(S.baumii)的相似性最高,仅有一个TPS单结构域;SvTPS二级结构中α-螺旋、β-折叠、延伸链、无规卷曲占比分别为48.61%、5.56%、15.62%、30.21%。三年生子实体SvTPS的表达量最高,菌丝体的表达量最低。菌丝体的SvTPS活性最低;随着栽培时间增加,SvTPS活性增加,三年生子实体的最高。研究结果为进一步探讨SvTPS在杨树桑黄生长发育过程中的功能提供参考。

Clinical analysis between serum 25-hydroxyvitamin D3, interleukin-6 levels and febrile convulsion in children

Objective:To investigate the relation between febrile convulsions and 25hydroxy-vitamin D_(3)[25-(OH)D_(3)]and interleukin-6(IL-6)levels in children.Methods:241 children(divided into simple febrile convulsions and complex febrile convulsions),who were diagnosed with febrile convulsions at the Women and Children's Medical Center of Hainan Province from January 2017 to October 2022,were selected into the febrile convulsions group;100 healthy children,who had no uncomfortable symptoms and attended the outpatient clinic of the Women and Children's Medical Center of Hainan Province for physical examination,for the control group.All the subjects measured the serum 25-(OH)D_(3) and IL-6 levels,and clinical information,such as age,gender and season,was recorded.Results:1)Serum 25-(OH)D_(3) levels in the febrile convulsion group were significantly lower than in the healthy control group(78.77±20.37 nmol/L versus 96.55±29.74 nmol/L,respectively),and there was a statistically significant between the two groups(t value-6.359,P<0.001).Serum IL-6 levels in the febrile convulsion group were significantly higher than in the healthy control group,and there was a statistically significant between the two groups(Z value of-14.291,P<0.001).2)Serum 25-(OH)D_(3) levels in children with complex febrile convulsions were significantly lower than those in children with simple febrile convulsions,and the difference between the two groups was statistically significant(t-value of 6.612,P<0.05).IL-6 levels were higher in children with complex febrile convulsions than in children with simple febrile convulsions,and the difference between the two groups was statistically significant(Z value-10.151,P<0.001).The difference in the severity of febrile convulsions was statistically significant in serum 25-(OH)D_(3) levels(x^(2)=29.83,P<0.001).3)The results of correlation analysis showed that serum 25-(OH)D_(3) level was negatively correlated with febrile convulsion(γ=-0.393,P<0.05);serum 25-(OH)D_(3) level was positively correlated with that(γs=0.328,P<0.05).4)The correlation analysis results showed that the serum 25-(OH)D_(3) level was negatively correlated with the clinical characteristics of febrile convulsion(γ=-0.393,P<0.05).However,serum IL-6 water is positively correlated with it(γs=0.328,P<0.05).4)In contrast,there was no statistically significant difference in serum 25-(OH)D_(3) levels among children with febrile convulsions in different seasons(P>0.05).Conclusions:There is a correlation between febrile convulsion and serum levels of 25-(OH)D_(3) and IL-6.25-(OH)D_(3) and IL-6 may participate in the pathogenesis of febrile convulsion.

Maternal TMPRSS6 Gene Polymorphism rs855791SNP in Women with Preeclampsia

Introduction: Preeclampsia can lead to several maternal and perinatal adverse effects. There are few published data on the association between transmembrane serine protease 6 (TMPRSS6) gene polymorphism and preeclampsia. Objective: To assess the association between TMPRSS6 gene polymorphism rs855791SNP in women with preeclampsia compared with healthy pregnant women. Method: A case-control study (60 women in each arm) was conducted at Saad Abuaela Maternity Hospital in Khartoum, Sudan. Sociodemographic and clinical data were gathered through a questionnaire. The participant was genotype for TMPRSS6 gene rs855791SNP using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The results were confirmed by DNA sequencing. Result: There was no significant difference in the median of age, parity, and body mass index. The distribution of the genotypes and alleles of TMPRSS6 rs855791 was consistent with the HWE. The overall TMPRSS6 rs855791 polymorphism was not significantly associated with preeclampsia. However, the proportion of heterozygotes (TC) was considerably higher in the women with preeclampsia (46.7%) than in the control group (23.3%) (p = 0.001;OR = 2.71;95% CI = 1.21 - 6.07). The proportion of homozygotes (TT) and T alleles was not significantly different between women with preeclampsia and the control group. Conclusion: The overall TMPRSS6 rs855791 polymorphism was not significantly associated with preeclampsia and healthy control.

过表达丝裂原诱导基因6通过抑制内质网应激减少棕榈酸诱导的肝细胞脂质蓄积

目的:探讨过表达丝裂原诱导基因6(MIG6)通过调节肝细胞内质网应激影响棕榈酸(PA)诱导的肝细胞脂肪变性的作用及其机制。方法:通过使用高脂饲料喂养20只C57BL/6J小鼠26周构建小鼠非酒精性脂肪性肝炎模型,将小鼠随机分为2组:普通饮食对照组和高脂饮食组,每组10只,留取其肝脏进行后续实验。使用PA诱导HepG2细胞和AML12细胞的脂质蓄积,将细胞分为2组:BSA(10%BSA)组和PA(500μmol/L)组。通过给HepG2细胞和AML12细胞分别转染人和小鼠MIG6过表达质粒诱导肝细胞MIG6过表达,根据不同的干预条件分为4组:阴性对照质粒+10%BSA(negative+BSA)组、MIG6过表达质粒+10%BSA(MIG6+BSA)组、阴性对照质粒+PA(negative+PA)组和MIG6过表达质粒+PA(MIG6+PA)组,每组每次干预至少设3个生物学复孔。使用油红O染色评估肝细胞脂质蓄积情况;通过RT-qPCR检测肝组织MIG6的mRNA表达水平,Western blot检测肝细胞MIG6、脂肪酸合酶(FASN)和胆固醇调节元件结合蛋白1(SREBP1)等脂质合成相关分子及内质网应激标志物葡萄糖调节蛋白78(GRP78)和C/EBP同源蛋白(CHOP)的蛋白水平。结果:高脂饮食组小鼠肝组织甘油三酯含量增加,肝组织脂肪变性显著,MIG6的mRNA表达水平显著下调(P<0.01);PA干预促使肝细胞FASN、SREBP1、GRP78和CHOP蛋白水平升高,但是降低MIG6蛋白水平(P<0.01)。转染MIG6过表达质粒显著增加肝细胞MIG6蛋白水平,并抑制GRP78和CHOP的表达(P<0.01),显著减轻PA诱导的肝细胞脂质蓄积并抑制FASN和SREBP1的表达(P<0.01)。以上结果表明过表达MIG6可抑制PA诱导的肝细胞内质网应激并减少其脂质蓄积。结论:过表达MIG6具有抑制内质网应激进而抑制肝细胞脂质蓄积的作用。

Association between Metabolic Syndrome Components and Serum High-Sensitivity C-Reactive Protein or Interleukin-6 Levels among Congolese Adults

Metabolic syndrome (MetS) and its components have been linked to elevated serum levels of inflammatory biomarkers such as C-reactive protein, interleukin-6, interleukin-1β and tumor necrosis factor alpha. The aim of our study was to address the association between MetS components with serum hs-CRP and IL-6 levels among Congolese adults. A total of 357 participants (aged 30 - 87 years) were included in this cross-sectional study. Anthropometrics were collected and fasting blood sampled for assessment of fasting blood glycaemia (FBG), lipids and inflammatory parameters using commercially available assays. NCEP-ATPIII criteria were used to define MetS. The Median (IQR) hs-CRP and IL-6 levels were higher in participants with MetS than in those without ([7 (4, 14) versus 6 (4, 8)] mg/L;p = 0.092 and [23.8 (20.9, 27.6) versus 22.3 (19.5, 25.0)] pg/mL;p = 0.002). hs-CRP and IL-6 levels were significantly higher in females with MetS than in those without, but not in males. Among participants, only TG was correlated with hs-CRP (r = 0.149, p = 0.007), and a significant correlation was observed between TG (r = 0.116, p = 0.037), FBG (r = 0.208, p = 0.000), HDL-C (r = −0.119, p = 0.034) and SBP (r = 0.143, p = 0.010) and IL-6. In males, hs-CRP levels were positively correlated with TG (0.316;p = 0.000), negatively with HDL-C (r = −0.290, p = 0.0022), without such correlations in females. In Ames, IL-6 levels were positively correlated with FBG (r = 0.202;p = 0.035), and negatively with HDL-C (r = −0.249, p = 0.009). Significant correlations between IL-6 levels and FBG (r = 0.214;p = 0.000) or SBP (r = 0.227, p = 0.000) were observed in females. Logistic regression analysis was carried out to identify the relationship between MetS components and hs-CRP or IL-6. Values of area under receiver-operating characteristic (ROC) curves suggest potential use of serum hs-CRP (AUC = 0.675) and IL-6 (AUC = 0.656) as diagnostic biomarkers of MetS. Combination of hs-CRP and IL-6 improved diagnosis accuracy, yielding a 0.698 ROC curve area. MetS components are associated with hs-CRP and IL-6 levels among adults Congolese. Combining the two biomarkers hs-CRP and IL-6 improves Mets diagnostic accuracy compared to hs-CRP or IL-6 alone.