Lobetyolin(LBT)is a polyacetylene glycoside found in diverse medicinal plants but mainly isolated from the roots of Codo-nopsis pilosula,known as Radix Codonopsis or Dangshen.Twelve traditional Chinese medicinal prepa...Lobetyolin(LBT)is a polyacetylene glycoside found in diverse medicinal plants but mainly isolated from the roots of Codo-nopsis pilosula,known as Radix Codonopsis or Dangshen.Twelve traditional Chinese medicinal preparations containing Radix Codonopsis were identified;they are generally used to tonify spleen and lung Qi and occasionally to treat cancer.Here we have reviewed the anticancer properties of Codonopsis extracts,LBT and structural analogs.Lobetyolin and lobetyolinin are the mono-and bis-glucosylated forms of the polyacetylenic compound lobetyol.Lobetyol and LBT have shown activi-ties against several types of cancer(notably gastric cancer)and we examined the molecular basis of their activity.A down-regulation of glutamine metabolism by LBT has been evidenced,contributing to drug-induced apoptosis and tumor growth inhibition.LBT markedly reduces both mRNA and protein expression of the amino acid transporter Alanine-Serine-Cysteine Transporter 2(ASCT2).Other potential targets are proposed here,based on the structural analogy with other anticancer compounds.LBT and related polyacetylene glycosides should be further considered as potential anticancer agents,but more work is needed to evaluate their efficacy,toxicity,and risk-benefit ratio.展开更多
Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhanc...Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhance the function of the stomach and spleen.However,there is limited research on the technology and pharmacodynamic effects of Tangshen fried with bran.Therefore,the objective of this article is to optimize the method of stir-frying with bran for Tangshen processing and to compare the anti-fatigue and hypoxia-resistance effects of Tangshen before and after bran-frying.Methods:The response surface methodology was utilized to optimize the frying temperature,frying time,and the bran-to-Tangshen ratio,using lobetyolin as the indicator.The swimming time of mice and the levels of blood urea nitrogen,lactate dehydrogenase,and blood glucose in fatigued mice were compared before and after Tangshen was fried with bran.Additionally,the hypoxic resistance ability of Tangshen before and after bran-frying was determined through normobaric hypoxia test and sodium nitrite poisoning test.Results:The optimal bran-frying conditions for Tangshen processing were determined as:frying temperature of 167°C,frying time of 2.67 min,and the bran-to-Tangshen ratio of 50:100.Pharmacodynamic analysis results indicated that Tangshen significantly increased the GLU content(P<0.01)and significantly reduced the LDH content(P<0.01)both before and after bran-frying.While both traditional and new bran-frying methods significantly increased the content of muscle glycogen(P<0.05),the optimized method in this study also significantly elevated the content of liver glycogen(P<0.05).The traditional method significantly prolonged the survival time of mice from sodium nitrite poisoning(P<0.05).Conclusion:The response surface model proved to be highly effective in optimizing the stir-frying process with bran for Tangshen processing.The results of this study indicate that Tangshen possesses anti-fatigue and hypoxia-resistance effects both before and after bran-frying,with bran-fried Tangshen exhibiting significantly stronger effects.展开更多
[Objective] This study aimed to establish the standards for quality control of Qishen oral solution. [Method] Astragalus membranaceus, Atractylodes macro- cephala Koidz. and Glycyrrhiza uralensis in the preparation we...[Objective] This study aimed to establish the standards for quality control of Qishen oral solution. [Method] Astragalus membranaceus, Atractylodes macro- cephala Koidz. and Glycyrrhiza uralensis in the preparation were identified by using thin layer chromatography (TLC), and Codonopsis pilosula was identified by using high-performance liquid chromatography (HPLC). [Result] By using the developed TLC system, experimental solution and reference solution showed clear spots, while negative control presented no interference. By using the developed HPLC system, the chromatographic peak of Iobetyolin was detected in experimental solution. [Con- clusion] The developed TLC and HPLC systems presented high specificity and good repeatability for identification of these four components and could be used in the quality control of Qishen oral solution.展开更多
文摘Lobetyolin(LBT)is a polyacetylene glycoside found in diverse medicinal plants but mainly isolated from the roots of Codo-nopsis pilosula,known as Radix Codonopsis or Dangshen.Twelve traditional Chinese medicinal preparations containing Radix Codonopsis were identified;they are generally used to tonify spleen and lung Qi and occasionally to treat cancer.Here we have reviewed the anticancer properties of Codonopsis extracts,LBT and structural analogs.Lobetyolin and lobetyolinin are the mono-and bis-glucosylated forms of the polyacetylenic compound lobetyol.Lobetyol and LBT have shown activi-ties against several types of cancer(notably gastric cancer)and we examined the molecular basis of their activity.A down-regulation of glutamine metabolism by LBT has been evidenced,contributing to drug-induced apoptosis and tumor growth inhibition.LBT markedly reduces both mRNA and protein expression of the amino acid transporter Alanine-Serine-Cysteine Transporter 2(ASCT2).Other potential targets are proposed here,based on the structural analogy with other anticancer compounds.LBT and related polyacetylene glycosides should be further considered as potential anticancer agents,but more work is needed to evaluate their efficacy,toxicity,and risk-benefit ratio.
基金This work was supported by the Key Project of the Key R&D Program of the Department of Science and Technology of Shanxi Province(201603D3112002).
文摘Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhance the function of the stomach and spleen.However,there is limited research on the technology and pharmacodynamic effects of Tangshen fried with bran.Therefore,the objective of this article is to optimize the method of stir-frying with bran for Tangshen processing and to compare the anti-fatigue and hypoxia-resistance effects of Tangshen before and after bran-frying.Methods:The response surface methodology was utilized to optimize the frying temperature,frying time,and the bran-to-Tangshen ratio,using lobetyolin as the indicator.The swimming time of mice and the levels of blood urea nitrogen,lactate dehydrogenase,and blood glucose in fatigued mice were compared before and after Tangshen was fried with bran.Additionally,the hypoxic resistance ability of Tangshen before and after bran-frying was determined through normobaric hypoxia test and sodium nitrite poisoning test.Results:The optimal bran-frying conditions for Tangshen processing were determined as:frying temperature of 167°C,frying time of 2.67 min,and the bran-to-Tangshen ratio of 50:100.Pharmacodynamic analysis results indicated that Tangshen significantly increased the GLU content(P<0.01)and significantly reduced the LDH content(P<0.01)both before and after bran-frying.While both traditional and new bran-frying methods significantly increased the content of muscle glycogen(P<0.05),the optimized method in this study also significantly elevated the content of liver glycogen(P<0.05).The traditional method significantly prolonged the survival time of mice from sodium nitrite poisoning(P<0.05).Conclusion:The response surface model proved to be highly effective in optimizing the stir-frying process with bran for Tangshen processing.The results of this study indicate that Tangshen possesses anti-fatigue and hypoxia-resistance effects both before and after bran-frying,with bran-fried Tangshen exhibiting significantly stronger effects.
基金Supported by Youth Fund of Beijing University of Agriculture(KM2013002)Scientific and Technological Project in Rural Areas from "Twelfth Five-Year Plan(2011BAD34B03-5)~~
文摘[Objective] This study aimed to establish the standards for quality control of Qishen oral solution. [Method] Astragalus membranaceus, Atractylodes macro- cephala Koidz. and Glycyrrhiza uralensis in the preparation were identified by using thin layer chromatography (TLC), and Codonopsis pilosula was identified by using high-performance liquid chromatography (HPLC). [Result] By using the developed TLC system, experimental solution and reference solution showed clear spots, while negative control presented no interference. By using the developed HPLC system, the chromatographic peak of Iobetyolin was detected in experimental solution. [Con- clusion] The developed TLC and HPLC systems presented high specificity and good repeatability for identification of these four components and could be used in the quality control of Qishen oral solution.