Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with t...Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with tubes containing normal broth only.Microaerophilic environment was also provided in a petri plate seeded with Campylobacter culture by pouring 3 different concentrations（10%,5%and 2.5%） of five reducing agents along with bacto-agar in the lid which was used to cover and seal the culture plate.Six reducing agents were also added in broth in concentration of 0.25 mg/mL of each with different combinations.Results:In lid agar technique,Campylobacter jejuni growth appeared in all three concentrations of reducing agents, that is 10%,5%and 2.5%after 24 hours of incubation but the best results were observed in 10% concentration.The colonial and morphological characters were not affected when the organisms were grown by this technique.Conclusions:It was found that reducing agents enhance the growth of C.jejuni/coli.In combination of FeSO<sub>4</sub>,Na<sub>2</sub>CO<sub>3</sub> with H<sub>3</sub>BO<sub>3</sub> worked as ideal mixture for the aerobic growth of Campylobacter.This technique is more economical as compared to commercially available media in the market and can be used for the oral facultative and microaerophilic bacterial growth in laboratory.展开更多
Strictly anaerobic bacteria in the evolutionary way formed some mechanisms of oxygen tolerance. These changes enable strictly anaerobic bacteria existing in natural environment in which avoiding oxygen is not possible...Strictly anaerobic bacteria in the evolutionary way formed some mechanisms of oxygen tolerance. These changes enable strictly anaerobic bacteria existing in natural environment in which avoiding oxygen is not possible. Clostridium bifermentans is described as a strictly anaerobe species;however, in the literature, there is some information about its oxygen tolerance. Thus, in this work, the level of C. bifermentans aero-tolerance, its mechanisms, and the ability to metabolite production in presence of oxygen in cultivation medium were investigated. It was found out that C. bifermentans is able to survive in the presence of oxygen. Moreover, they are able to utilize oxygen and product metabolites when the level of oxygen is below 10%. In bacteria cells superoxide dismutase was detected.展开更多
文摘Objective:To produce a technique for the growth of Campylobacter jejuni in aerobic condition Methods:Different combinations of reducing agents were tested in brucella broth and the growth turbidity was compared with tubes containing normal broth only.Microaerophilic environment was also provided in a petri plate seeded with Campylobacter culture by pouring 3 different concentrations（10%,5%and 2.5%） of five reducing agents along with bacto-agar in the lid which was used to cover and seal the culture plate.Six reducing agents were also added in broth in concentration of 0.25 mg/mL of each with different combinations.Results:In lid agar technique,Campylobacter jejuni growth appeared in all three concentrations of reducing agents, that is 10%,5%and 2.5%after 24 hours of incubation but the best results were observed in 10% concentration.The colonial and morphological characters were not affected when the organisms were grown by this technique.Conclusions:It was found that reducing agents enhance the growth of C.jejuni/coli.In combination of FeSO<sub>4</sub>,Na<sub>2</sub>CO<sub>3</sub> with H<sub>3</sub>BO<sub>3</sub> worked as ideal mixture for the aerobic growth of Campylobacter.This technique is more economical as compared to commercially available media in the market and can be used for the oral facultative and microaerophilic bacterial growth in laboratory.
文摘Strictly anaerobic bacteria in the evolutionary way formed some mechanisms of oxygen tolerance. These changes enable strictly anaerobic bacteria existing in natural environment in which avoiding oxygen is not possible. Clostridium bifermentans is described as a strictly anaerobe species;however, in the literature, there is some information about its oxygen tolerance. Thus, in this work, the level of C. bifermentans aero-tolerance, its mechanisms, and the ability to metabolite production in presence of oxygen in cultivation medium were investigated. It was found out that C. bifermentans is able to survive in the presence of oxygen. Moreover, they are able to utilize oxygen and product metabolites when the level of oxygen is below 10%. In bacteria cells superoxide dismutase was detected.
