To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we...To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation（SHG） microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG image.Further, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.展开更多
Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency ...Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency (Condic, 2014). In mice,only zygotes and blastomeres of 2-cell stage embryos are considered totipotent, since they have the ability to develop into a full展开更多
基金supported by the National Key Research and Development Program of China(No.2016YFA0201403)the National Natural Science Foundation of China(No.61522502)the Science Fund for Creative Research Group of China(No.61421064)
文摘To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation（SHG） microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG image.Further, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.
基金supported by the National Natural Science Foundation of China (Nos. 31621004 and 81571356)the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY-SSWSMC002)+1 种基金the Key Deployment Projects of the Chinese Academy of Sciences (ZDRW-ZS-2017-5)the Youth Innovation Promotion Association CAS (No. 2017113)
文摘Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency (Condic, 2014). In mice,only zygotes and blastomeres of 2-cell stage embryos are considered totipotent, since they have the ability to develop into a full
