A comprehensive analysis is conducted based on observations on topography, tidal current, salinity, suspended sediment and bed load during the years of 1982, 1983, 1988, 1989. 1996, and 1997 in the Yangtze Estuary. Re...A comprehensive analysis is conducted based on observations on topography, tidal current, salinity, suspended sediment and bed load during the years of 1982, 1983, 1988, 1989. 1996, and 1997 in the Yangtze Estuary. Results show that the deformation of tidal waves is distinct and the sand carrying capacity is large within the mouth bar due to strong tidal currents and large volume of incoming water and sediments. Owing to both temporal and spatial variation of tidal current, deposition and erosion ore extremely active. In general a change of up to 0.1 m of bottom sediments takes place during a tidal period. The maximum siltation and erosion are around 0.2 m in a spring to neap tides cycle. The riverbed is silted during flood when there is heavy sediment load, eroded during dry season when sediment lo:ld is low. The annual average depth of erosion anti siltation on the riverbed is around 0.6 m. In particular cases, it may increase to 1.4 m to 2.4 m at some locations.展开更多
On the basis of the bathymetric records of 371 survey sections in the present Huanghe mouth area from 1996 to 2001, the temporal and spatial variations of the Huanghe mouth bars were studied by using GIS technology. T...On the basis of the bathymetric records of 371 survey sections in the present Huanghe mouth area from 1996 to 2001, the temporal and spatial variations of the Huanghe mouth bars were studied by using GIS technology. The variation of the mouth bars is closely related to the water and sediment discharges from the Huanghe River to the sea that has been decreasing drastically in recent years, and to coastal hydrodynamic regimes. The characteristics of the mouth bars are unique in contrast with those of other estuaries in the world. (1) The mouth bars of the Huanghe River consist of many small sandbars. Usually the sandbars are of ellipse-shape with the long axis of about 1~6 km, and short axis of about 1~4 km. The long axis is parallel to the principal direction of tidal current, and the short axis is variable, depending on the local dynamics. The crest of the sandbars is only about 0.4 m below the water surface at low tide. They are distributed within an area of 20 km2, not far from the river mouth area. The present mouth bars are in small-medium size, quite different from the large one formed during the 1970s and 1980s, when the river sediment discharge was several times larger than that after 1996. (2) The scale of a river mouth bar is related to the river discharge of that year. In 1997 sediment discharge from the Huanghe River was 42 Mt, and the mouth bar consisted of several small scattered sandbars. In 1998 the size of mouth bars was much larger than that in 1997, as the sediment discharge from the Huanghe River increased to 363 Mt. Variation of the mouth bars largely depends on the sediment discharge with a correlation coefficient of 0.78. The calculation of the volume of the mouth bar area shows that about 30%~40% of the river's into-sea sediment is deposited in the mouth bar area. (3) Compared with other large estuaries in the world, the Huanghe mouth bars have three unique features in responding to its high sediment concentration: small size, steep slope gradient and shallow crest depth.展开更多
Field observational previously indicated a mouth bar of a fan delta could exhibit a fining-or coarseningupward trend,which bring a new challenge to the identification of mouth bar in subsurface studies due to the lack...Field observational previously indicated a mouth bar of a fan delta could exhibit a fining-or coarseningupward trend,which bring a new challenge to the identification of mouth bar in subsurface studies due to the lack of morphological descriptions.Previous studies have indicated that effluent behavior in rivermouth system can affect the vertical grain-size trend of mouth bar,but the drivers and magnitude of this phenomenon are not understood.We conducted flume experiments to investigate the mechanism and controlling factors of vertical grain-size trend of mouth bar.Experiment with a steeper slope of the substrate layer,greater discharge,higher sediment/water ratio,and coarser sediment induced a finingupward trend of mouth bar,because the effluent was dominated by strong inertia.Mouth bar in the experiment with a gentler slope of the substrate layer,smaller discharge,lower sediment/water ratio,and finer sediment exhibited a coarsening-upward trend dominated by the friction-dominated effluent.The relationship between the vertical grain-size trend of mouth bar and the gradients of foreset bedding in small-scale flume models and the cut-off of 15°-18°are applicable in natural systems.Identifying depositional setting to infer depositional process in river-mouth system and analyzing the plane geometry of sandbodies are two steps in the interpretation of ancient fan deltaic rock record.展开更多
-Based on the feasibility study of developing the navigation resources of the Guanhe River and the model test results of the mouth bar regulation, this paper presents some basic principles for the regulation of the ch...-Based on the feasibility study of developing the navigation resources of the Guanhe River and the model test results of the mouth bar regulation, this paper presents some basic principles for the regulation of the channel on the mouth bar, for instance, the direction of navigation channel should be identical with that of the ebb tide current and the main waves, and perpendicular to the bathymetric contours. The principles for regulating mouth bars are also discussed in this paper.展开更多
Based on the characteristics of hydrodynamics and sediment transport in the bar area in the Modaomen Estuary,a flume experiment was performed to study the evolution of the longitudinal profile of the mouth bar.The mou...Based on the characteristics of hydrodynamics and sediment transport in the bar area in the Modaomen Estuary,a flume experiment was performed to study the evolution of the longitudinal profile of the mouth bar.The mouth bar evolution was investigated under the impacts of floods with different return periods as well as flood-wave interaction.The results showed that floods with different return periods had significant influences on the evolution of the river mouth bar.Particularly on the inner slope of the mouth bar,the sediment was substantially active and moveable.The inner slope and the bar crest tended to be remarkably scoured.The erosion was intensified with the increase of the magnitude of floods.Moreover,the bar crest moved seawards,while the elevation of the bar crest barely changed.Under the flood-wave interaction,a remarkable amount of erosion on the inner and outer slopes of the mouth bar was also found.The seaward displacement of the bar crest under the interaction of floods and waves was less than it was under only the impact of floods,while more deposition was found on the crest of the mouth bar in this case.展开更多
Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and ...Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and minerals were characterized microscopically,the measured trap sealing indexes were compared,the biomarker compounds of crude oil were extracted,the genesis of condensate gas was identified,and the reservoir-forming conditions were examined.On this basis,the Paleogene Enping Formation in the Huizhou 26 subsag was systematically analyzed for the potential of oil and gas resources,the development characteristics of large-scale high-quality conglomerate reservoirs,the trapping effectiveness of faults,the hydrocarbon migration and accumulation model,and the formation conditions and exploration targets of large-and medium-sized glutenite-rich oil and gas fields.The research results were obtained in four aspects.First,the Paleogene Wenchang Formation in the Huizhou 26 subsag develops extensive and thick high-quality source rocks of semi-deep to deep lacustrine subfacies,which have typical hydrocarbon expulsion characteristics of"great oil generation in the early stage and huge gas expulsion in the late stage",providing a sufficient material basis for hydrocarbon accumulation in the Enping Formation.Second,under the joint control of the steep slope zone and transition zone of the fault within the sag,the large-scale near-source glutenite reservoirs are highly heterogeneous,with the development scale dominated hierarchically by three factors(favorable facies zone,particle component,and microfracture).The(subaqueous)distributary channels near the fault system,with equal grains,a low mud content(<5%),and a high content of feldspar composition,are conducive to the development of sweet spot reservoirs.Third,the strike-slip pressurization trap covered by stable lake flooding mudstone is a necessary condition for oil and gas preservation,and the NE and nearly EW faults obliquely to the principal stress have the best control on traps.Fourth,the spatiotemporal configuration of high-quality source rocks,fault transport/sealing,and glutenite reservoirs controls the degree of hydrocarbon enrichment.From top to bottom,three hydrocarbon accumulation units,i.e.low-fill zone,transition zone,and high-fill zone,are recognized.The main area of the channel in the nearly pressurized source-connecting fault zone is favorable for large-scale hydrocarbon enrichment.The research results suggest a new direction for the exploration of large-scale glutenite-rich reservoirs in the Enping Formation of the Pearl River Mouth Basin,and present a major breakthrough in oil and gas exploration.展开更多
The mouth bar in the Yangtze estuarine waterways has a significant influence on navigational transport within the estuary, flood discharge and construction of the Shanghai Port. In this paper the morphological evoluti...The mouth bar in the Yangtze estuarine waterways has a significant influence on navigational transport within the estuary, flood discharge and construction of the Shanghai Port. In this paper the morphological evolution and mechanisms of mouth bar formation of the Yangtze estuarine waterways are studied by analyzing hundreds of years of historical data and the latest profile maps of some or the main mouth bar channels in the Yangtze Estuary. The results are shown as follows: The mouth bars in the North Branch have moved gradually from outside the mouth to the inside and formed a huge sand bar. In the North Channel, the head of the mouth bar has migrated about 30 kilometers downstream, and a channel bar has been developing since 2001. Two mouth bar tops, which always existed in the North Passage disappeared in 2010. The head of the mouth bar in the South Passage has migrated downstream about 14 km and the number of tops increased at first but is reduced to only one now. According to the results, we can conclude that the evolution of the mouth bars differs depending on their location. In the North Branch it is directly related to large-scale reclamation in Chongming Island, but in the North Passage it has a close relationship with regulation of the Yangtze Estuary Deepwater Channel. However, the evolution of mouth bars in the North Channel and South Passage is not only connected with the Yangtze Estuary Deepwater Channel Regulation Project, but also with the reclamation in the East Hengsha Shoal and the closure of the Qingcaosha Reservoir.展开更多
The coupling relationship between shelf-edge deltas and deep-water fan sand bodies is a hot and cutting-edge field of international sedimentology and deep-water oil and gas exploration.Based on the newly acquired high...The coupling relationship between shelf-edge deltas and deep-water fan sand bodies is a hot and cutting-edge field of international sedimentology and deep-water oil and gas exploration.Based on the newly acquired high-resolution 3D seismic,logging and core data of Pearl River Mouth Basin(PRMB),this paper dissected the shelf-edge delta to deep-water fan(SEDDF)depositional system in the Oligocene Zhuhai Formation of Paleogene in south subsag of Baiyun Sag,and revealed the complex coupling relationship from the continental shelf edge to deep-water fan sedimentation and its genetic mechanisms.The results show that during the deposition of the fourth to first members of the Zhuhai Formation,the scale of the SEDDF depositional system in the study area showed a pattern of first increasing and then decreasing,with deep-water fan developed in the third to first members and the largest plane distribution scale developed in the late stage of the second member.Based on the development of SEDDF depositional system along the source direction,three types of coupling relationships are divided,namely,deltas that are linked downdip to fans,deltas that lack downdip fans and fans that lack updip coeval deltas,with different depositional characteristics and genetic mechanisms.(1)Deltas that are linked downdip to fans:with the development of shelf-edge deltas in the shelf area and deep-water fans in the downdip slope area,and the strong source supply and relative sea level decline are the two key factors which control the development of this type of source-to-sink(S2S).The development of channels on the continental shelf edge is conducive to the formation of this type of S2S system even with weak source supply and high sea level.(2)Deltas that lack downdip fans:with the development of shelf edge deltas in shelf area,while deep water fans are not developed in the downdip slope area.The lack of“sources”and“channels”,and fluid transformation are the three main reasons for the formation of this type of S2S system.(3)Fans that lack updip coeval deltas:with the development of deep-water fans in continental slope area and the absence of updip coeval shelf edge deltas,which is jointly controlled by the coupling of fluid transformation at the shelf edge and the“channels”in the continental slope area.展开更多
A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon...A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon accumulation conditions,the geometry,kinematics,and reservoir control of a large synthetic overlapping transfer zone in the south of the Wenchang A subsag in the Zhujiang(Pearl)River Mouth basin were investigated.Results indicate that the development and evolution of the transfer zone was controlled by the interaction between pre-existing faults and regional stress transformation.The intense rifting of the main faults of the transfer zone controlled the development of source rocks and faultcontrolled slope break paleogeomorphology.The strike-slip overprint since the Oligocene is conducive to the formation of a large-scale fault-anticline trap,and the secondary faults in the transfer zone contribute to the hydrocarbon transportation.The conjugate intersection area of the NE-and NW-trending faults offers more opportunity for hydrocarbon migration and accumulation.展开更多
Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift ...Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift normal fault through detailed seismic interpretation and fault modeling.A total of 289 post-rift normal faults were identified in the study area and can be classified into four types:(1)isolated normal faults above the carbonate platform;(2)isolated normal faults cutting through the carbonate platform;(3)conjugate normal faults,and(4)connecting normal faults.Throw distribution analysis on the fault planes show that the vertical throw profiles of most normal fault exhibit flat-topped profiles.Isolated normal faults above the carbonate platform exhibit roughly concentric ellipses with maximum throw zones in the central section whereas the normal faults cutting through the carbonate platform miss the lowermost section due to the chaotic seismic reflections in the interior of the carbonate platform.The vertical throws of conjugate normal faults anomalously decrease toward their intersection region on the fault plane whereas the connecting normal faults present two maximum throw zones in the central section of the fault plane.According to the symmetric elliptical distribution model of fault throw,an estimation was made indicating that normal faults cutting through the carbonate platform extended downward between-1308 s and-1780 s(two-way travel time)in depth and may not penetrate the entire Liuhua carbonate platform.Moreover,it is observed that the distribution of karst caves on the top of the carbonate platform disaccord with those of hydrocarbon reservoirs and the post-rift normal faults cutting through the carbonate platform in the study area.We propose that these karst caves formed most probably by corrosive fluids derived from magmatic activities during the Dongsha event,rather than pore waters or hydrocarbons.展开更多
Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR...Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells(PBMCs)infected with enterovirus 71(EV-A71)which were derived from patients with HFMD of different severities and at different stages.A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates.Subsequently,ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes,specifically,BST2,IRF7,IFI16,TRIM21,MX1,and DDX58.Results:Compared with those at the recovery stage,the expression levels of BST2(P=0.027),IFI16(P=0.016),MX1(P=0.046)and DDX58(P=0.008)in the acute stage of infection were significantly upregulated,while no significant difference in the expression levels of IRF7(P=0.495)and TRIM21(P=0.071)was found between different stages of the disease.The expression levels of BST2,IRF7,IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens,and BST2,IRF7,IFI16 and MX1 expression levels were significantly lower in coxsackie B virus(COXB)positive patients than the negative patients.Expression levels of one or more of BST2,IRF7,IFI16,TRIM21,MX1 and DDX58 genes were correlated with PCT levels,various white blood cell counts,and serum antibody levels that reflect disease course of HFMD.Aspartate aminotransferase was correlated with BST2,MX1 and DDX58 expression levels.Conclusions:PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD.展开更多
Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient samp...Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient sampling, 60 patients with mouth-opening difficulty after treatment for head and neck cancer admitted to the Oncology Department from February 2022 to October 2023 were selected for a 2-week exercise and nursing program. The patients were divided into a control group (February 1, 2022 to November 30, 2022) and an observation group (December 1, 2022 to October 31, 2023), with 30 patients in each group. The control group underwent routine mouth-opening functional exercises combined with cork for oral support training, while the observation group underwent routine mouth-opening functional exercises combined with oral massage and mouth-opening training sticks for oral support training. The mouth-opening degree, mouth-opening difficulty level, comfort level, compliance, and quality of life were observed in both groups. Results: Before the intervention, there were no statistically significant differences in mouth-opening degree and mouth-opening difficulty level between the two groups (P > 0.05). After the intervention, the mouth-opening degree, mouth-opening difficulty level, oral comfort level, compliance, and QLICP-HN scores in the observation group were all better than those in the control group, with statistically significant differences (P < 0.05). Conclusion: The combination of mouth-opening training sticks and oral massage with mouth-opening functional exercises can effectively improve the degree of mouth-opening difficulty, enhance oral comfort, increase compliance with mouth-opening exercises, and improve quality of life.展开更多
Mixed siliciclastic-carbonate rocks constitute the Upper Cretaceous Garuda- mangalam Sandstone Formation, Ariyalur (India), and offer an opportunity to look into the broad spectrum of mixing of compositionally and g...Mixed siliciclastic-carbonate rocks constitute the Upper Cretaceous Garuda- mangalam Sandstone Formation, Ariyalur (India), and offer an opportunity to look into the broad spectrum of mixing of compositionally and genetically different components. The palaeogeographic reconstruction indicates that deposition in the nearshore zone differed strongly in energy and active processes operatives due to the presence of a shore-parallel river-mouth bar. The western wing of the Mississippi bird-foot delta is considered to be a present-day analogon. Facies analysis in combination with petrography clearly shows the variability in palaeoenvironmental characteristics, both biogenic and non-biogenic. It also indicates dia- genetic uptake of carbonate that filled empty spaces and actively replaced original compo- nents. Chemical staining followed by limited application of cathodoluminescence and energy dispersive X-ray analysis (EDAX) hint at intricacies in mixing arising from the compositional variations in the carbonate components. A model of siliciclastic-carbonate sediment mixing, including both the depositional and diagenetic developments, is presented; it is aimed at generating a better overview of, and a deeper insight into, the physical and chemical mechanisms involved.展开更多
River-mouth bar is a fundamental element to constitute a delta system, and its internal facies architectures are vital to reconstruct delta evolution history and study high-resolution sequence stratigraphy. Changxing ...River-mouth bar is a fundamental element to constitute a delta system, and its internal facies architectures are vital to reconstruct delta evolution history and study high-resolution sequence stratigraphy. Changxing Island is a representative mouth-bar complex in the modern Changjiang Delta. Its vertical stacked strata produced by delta progradation were first studied by detailed facies analysis of core CX03, and then a general facies architecture and evolution history of mouth-bar complexes in the modern Changjiang Delta were discussed through a comparative study of 5 different cores and historical charts. A progradational deltaic sequence has generally a similar internal facies architecture, composed of massive muddy deposits of prodelta and delta-front slope facies at the bottom, sand-dominated deposits of delta-front platform, inter-bar channel and mouth-bar facies at the middle, and heterolithic deposits of tidal flat and saltmarsh facies at the top. There is a significant time lag for the initial formation of channel-mouth bar systems between Chongming and Jiuduansha Islands. Compared with subtidal flats, sedimentation rates on intertidal flats were highly accelerated, potentially resulting from weakening wave and tidal strength over extensive intertidal flats and increasing human embankment activities in the last few centuries. These findings provide some new clues to understand modern and ancient mouth-bar evolution and resultant sequence strata in tide-dominated deltas.展开更多
Almost at every mouth of the tributary creek of the Three Gorges inthe Gezhouba Reservoir, the alluvial fans or alluvial cones formed the rapids, whichare called rapids of creek mouth--bar. They are composed of pebble...Almost at every mouth of the tributary creek of the Three Gorges inthe Gezhouba Reservoir, the alluvial fans or alluvial cones formed the rapids, whichare called rapids of creek mouth--bar. They are composed of pebble bed load with d>20 mm. Based upon geological and geomorphological investigations, the authors detect that the pebble bed load at the Gezhouba Re3ervoir comes from two sources,namely, the region mainly with limestone and The region mainly with pyrolith, andtheir converging area is within the Gezhouba Project. percentages of the pebble bedload are calculated. The calculated result stows that, 65% of pebble bed load comesfrom the region mainly with limestone, and 93 % of the rapids of creek mouth--bar isdistributed in the region mainly with limestone. Considering the water level fluctuations, the rapids of creek mouth--bar can be divided into 3 types i. e. flood waterones, medium water ones and low water ones. Due to the rise of water level afterbuilding the Gezhouba Reservoir, the navigable channels in the area of rapids ofcreek mouth--bar within varied back water region are improved, taking up 50% of all12 rapids. But the rapids of creek mouth--bar in the tail region where the water levelrises less than 1 m, the conditions of navigable channels are little improved and evendeterioration, because of the continuous supply of pebble bed load. Therefore, thoserapids must be regulated before the completion of the Three Gorges Project.展开更多
The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evoluti...The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evolution and for unraveling its poorly studied source-rock maturation history.Our investigations in this study are based on apatite fission-track(AFT)thermochronology analysis of 12 cutting samples from 4 boreholes.Both AFT ages and length data suggested that the PRMB has experienced quite complicated thermal evolution.Thermal history modeling results unraveled four successive events of heating separated by three stages of cooling since the early Middle Eocene.The cooling events occurred approximately in the Late Eocene,early Oligocene,and the Late Miocene,possibly attributed to the Zhuqiong II Event,Nanhai Event,and Dongsha Event,respectively.The erosion amount during the first cooling stage is roughly estimated to be about 455-712 m,with an erosion rate of 0.08-0.12 mm/a.The second erosion-driven cooling is stronger than the first one,with an erosion amount of about 747-814 m and an erosion rate between about 0.13-0.21 mm/a.The erosion amount calculated related to the third cooling event varies from 800 m to 3419 m,which is speculative due to the possible influence of the magmatic activity.展开更多
The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The par...The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The parameters we use in mathematical models are usually classical mathematical parameters,called crisp parameters,which are taken for granted.But any biological or physical phenomenon is best explained by uncertainty.To represent a realistic situation in any mathematical model,fuzzy parameters can be very useful.Many articles have been published on how to control and prevent HFMD from the perspective of public health and statistical modeling.However,few works use fuzzy theory in building models to simulateHFMDdynamics.In this context,we examined anHFMD model with fuzzy parameters.A Non Standard Finite Difference(NSFD)scheme is developed to solve the model.The developed technique retains essential properties such as positivity and dynamic consistency.Numerical simulations are presented to support the analytical results.The convergence and consistency of the proposed method are also discussed.The proposed method converges unconditionally while the many classical methods in the literature do not possess this property.In this regard,our proposed method can be considered as a reliable tool for studying the dynamics of HFMD.展开更多
EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was...EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.展开更多
Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 wer...Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 were selected for this study.The patients were divided into two groups of thirty cases each using the randomized numerical table method.The observation underwent psychological intervention and mindfulness meditation training,while the control group only received symptomatic care.The condition of the patients of both groups was observed and compared.Results:Upon receiving treatment,the patients in the observation group had lower Hamilton Anxiety(HAM-A)scores,and Hamilton Depression(HAMD)scores compared to the control group(P<0.05).The visual analog scale(VAS)scores of the observation group were also lower than those of the control group(P<0.05).Moreover,the efficacy of the nursing intervention in the observation group was higher than that of the control group(P<0.05).Conclusion:Psychological intervention and mindfulness meditation training can effectively improve the clinical symptoms of patients with burning mouth syndrome.Therefore,this treatment method should be popularized.展开更多
基金This study is supported by the National Natural Science Foundation of China(Grant No.49736220)
文摘A comprehensive analysis is conducted based on observations on topography, tidal current, salinity, suspended sediment and bed load during the years of 1982, 1983, 1988, 1989. 1996, and 1997 in the Yangtze Estuary. Results show that the deformation of tidal waves is distinct and the sand carrying capacity is large within the mouth bar due to strong tidal currents and large volume of incoming water and sediments. Owing to both temporal and spatial variation of tidal current, deposition and erosion ore extremely active. In general a change of up to 0.1 m of bottom sediments takes place during a tidal period. The maximum siltation and erosion are around 0.2 m in a spring to neap tides cycle. The riverbed is silted during flood when there is heavy sediment load, eroded during dry season when sediment lo:ld is low. The annual average depth of erosion anti siltation on the riverbed is around 0.6 m. In particular cases, it may increase to 1.4 m to 2.4 m at some locations.
基金supported by the National Natural Science Foundation Key Project of China under Contract No.90211022“973”Project of MST of China under contract No.2002CB412404.
文摘On the basis of the bathymetric records of 371 survey sections in the present Huanghe mouth area from 1996 to 2001, the temporal and spatial variations of the Huanghe mouth bars were studied by using GIS technology. The variation of the mouth bars is closely related to the water and sediment discharges from the Huanghe River to the sea that has been decreasing drastically in recent years, and to coastal hydrodynamic regimes. The characteristics of the mouth bars are unique in contrast with those of other estuaries in the world. (1) The mouth bars of the Huanghe River consist of many small sandbars. Usually the sandbars are of ellipse-shape with the long axis of about 1~6 km, and short axis of about 1~4 km. The long axis is parallel to the principal direction of tidal current, and the short axis is variable, depending on the local dynamics. The crest of the sandbars is only about 0.4 m below the water surface at low tide. They are distributed within an area of 20 km2, not far from the river mouth area. The present mouth bars are in small-medium size, quite different from the large one formed during the 1970s and 1980s, when the river sediment discharge was several times larger than that after 1996. (2) The scale of a river mouth bar is related to the river discharge of that year. In 1997 sediment discharge from the Huanghe River was 42 Mt, and the mouth bar consisted of several small scattered sandbars. In 1998 the size of mouth bars was much larger than that in 1997, as the sediment discharge from the Huanghe River increased to 363 Mt. Variation of the mouth bars largely depends on the sediment discharge with a correlation coefficient of 0.78. The calculation of the volume of the mouth bar area shows that about 30%~40% of the river's into-sea sediment is deposited in the mouth bar area. (3) Compared with other large estuaries in the world, the Huanghe mouth bars have three unique features in responding to its high sediment concentration: small size, steep slope gradient and shallow crest depth.
基金supported by the National Natural Science Foundation of China(No.41772101)。
文摘Field observational previously indicated a mouth bar of a fan delta could exhibit a fining-or coarseningupward trend,which bring a new challenge to the identification of mouth bar in subsurface studies due to the lack of morphological descriptions.Previous studies have indicated that effluent behavior in rivermouth system can affect the vertical grain-size trend of mouth bar,but the drivers and magnitude of this phenomenon are not understood.We conducted flume experiments to investigate the mechanism and controlling factors of vertical grain-size trend of mouth bar.Experiment with a steeper slope of the substrate layer,greater discharge,higher sediment/water ratio,and coarser sediment induced a finingupward trend of mouth bar,because the effluent was dominated by strong inertia.Mouth bar in the experiment with a gentler slope of the substrate layer,smaller discharge,lower sediment/water ratio,and finer sediment exhibited a coarsening-upward trend dominated by the friction-dominated effluent.The relationship between the vertical grain-size trend of mouth bar and the gradients of foreset bedding in small-scale flume models and the cut-off of 15°-18°are applicable in natural systems.Identifying depositional setting to infer depositional process in river-mouth system and analyzing the plane geometry of sandbodies are two steps in the interpretation of ancient fan deltaic rock record.
文摘-Based on the feasibility study of developing the navigation resources of the Guanhe River and the model test results of the mouth bar regulation, this paper presents some basic principles for the regulation of the channel on the mouth bar, for instance, the direction of navigation channel should be identical with that of the ebb tide current and the main waves, and perpendicular to the bathymetric contours. The principles for regulating mouth bars are also discussed in this paper.
基金supported by the Changjiang River Scientific Research Institute(CRSRI)Open Research Program(Grant No.CKWV2017499/KY)the National Natural Science Foundation of China(Grant No.51779280)
文摘Based on the characteristics of hydrodynamics and sediment transport in the bar area in the Modaomen Estuary,a flume experiment was performed to study the evolution of the longitudinal profile of the mouth bar.The mouth bar evolution was investigated under the impacts of floods with different return periods as well as flood-wave interaction.The results showed that floods with different return periods had significant influences on the evolution of the river mouth bar.Particularly on the inner slope of the mouth bar,the sediment was substantially active and moveable.The inner slope and the bar crest tended to be remarkably scoured.The erosion was intensified with the increase of the magnitude of floods.Moreover,the bar crest moved seawards,while the elevation of the bar crest barely changed.Under the flood-wave interaction,a remarkable amount of erosion on the inner and outer slopes of the mouth bar was also found.The seaward displacement of the bar crest under the interaction of floods and waves was less than it was under only the impact of floods,while more deposition was found on the crest of the mouth bar in this case.
基金Supported by the CNOOC Major Technology Project During the 14th FIVE-YEAR PLAN PERIOD(KJGG2022-0403)CNOOC Major Technology Project(KJZH-2021-0003-00).
文摘Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and minerals were characterized microscopically,the measured trap sealing indexes were compared,the biomarker compounds of crude oil were extracted,the genesis of condensate gas was identified,and the reservoir-forming conditions were examined.On this basis,the Paleogene Enping Formation in the Huizhou 26 subsag was systematically analyzed for the potential of oil and gas resources,the development characteristics of large-scale high-quality conglomerate reservoirs,the trapping effectiveness of faults,the hydrocarbon migration and accumulation model,and the formation conditions and exploration targets of large-and medium-sized glutenite-rich oil and gas fields.The research results were obtained in four aspects.First,the Paleogene Wenchang Formation in the Huizhou 26 subsag develops extensive and thick high-quality source rocks of semi-deep to deep lacustrine subfacies,which have typical hydrocarbon expulsion characteristics of"great oil generation in the early stage and huge gas expulsion in the late stage",providing a sufficient material basis for hydrocarbon accumulation in the Enping Formation.Second,under the joint control of the steep slope zone and transition zone of the fault within the sag,the large-scale near-source glutenite reservoirs are highly heterogeneous,with the development scale dominated hierarchically by three factors(favorable facies zone,particle component,and microfracture).The(subaqueous)distributary channels near the fault system,with equal grains,a low mud content(<5%),and a high content of feldspar composition,are conducive to the development of sweet spot reservoirs.Third,the strike-slip pressurization trap covered by stable lake flooding mudstone is a necessary condition for oil and gas preservation,and the NE and nearly EW faults obliquely to the principal stress have the best control on traps.Fourth,the spatiotemporal configuration of high-quality source rocks,fault transport/sealing,and glutenite reservoirs controls the degree of hydrocarbon enrichment.From top to bottom,three hydrocarbon accumulation units,i.e.low-fill zone,transition zone,and high-fill zone,are recognized.The main area of the channel in the nearly pressurized source-connecting fault zone is favorable for large-scale hydrocarbon enrichment.The research results suggest a new direction for the exploration of large-scale glutenite-rich reservoirs in the Enping Formation of the Pearl River Mouth Basin,and present a major breakthrough in oil and gas exploration.
基金National Natural Science Foundation of China,No.40776056Research Subject of Shanghai Association for Science & Technology,No.10dzl210600Fund from the Ministry of Science and Technology of China for the State Key Laboratory of Esmarine and Coastal Research.No.2008KYYW01 Acknowledgements I am heartily grateful to Jiang Hong, Wang Dongmei, Yang Zhongyong, Ni Yizhuo and Tana for their help during the process of writing the article.
文摘The mouth bar in the Yangtze estuarine waterways has a significant influence on navigational transport within the estuary, flood discharge and construction of the Shanghai Port. In this paper the morphological evolution and mechanisms of mouth bar formation of the Yangtze estuarine waterways are studied by analyzing hundreds of years of historical data and the latest profile maps of some or the main mouth bar channels in the Yangtze Estuary. The results are shown as follows: The mouth bars in the North Branch have moved gradually from outside the mouth to the inside and formed a huge sand bar. In the North Channel, the head of the mouth bar has migrated about 30 kilometers downstream, and a channel bar has been developing since 2001. Two mouth bar tops, which always existed in the North Passage disappeared in 2010. The head of the mouth bar in the South Passage has migrated downstream about 14 km and the number of tops increased at first but is reduced to only one now. According to the results, we can conclude that the evolution of the mouth bars differs depending on their location. In the North Branch it is directly related to large-scale reclamation in Chongming Island, but in the North Passage it has a close relationship with regulation of the Yangtze Estuary Deepwater Channel. However, the evolution of mouth bars in the North Channel and South Passage is not only connected with the Yangtze Estuary Deepwater Channel Regulation Project, but also with the reclamation in the East Hengsha Shoal and the closure of the Qingcaosha Reservoir.
基金Supported by the National Natural Science Foundation of China(91528303)CNOOC Technology Project(2021-KT-YXKY-05).
文摘The coupling relationship between shelf-edge deltas and deep-water fan sand bodies is a hot and cutting-edge field of international sedimentology and deep-water oil and gas exploration.Based on the newly acquired high-resolution 3D seismic,logging and core data of Pearl River Mouth Basin(PRMB),this paper dissected the shelf-edge delta to deep-water fan(SEDDF)depositional system in the Oligocene Zhuhai Formation of Paleogene in south subsag of Baiyun Sag,and revealed the complex coupling relationship from the continental shelf edge to deep-water fan sedimentation and its genetic mechanisms.The results show that during the deposition of the fourth to first members of the Zhuhai Formation,the scale of the SEDDF depositional system in the study area showed a pattern of first increasing and then decreasing,with deep-water fan developed in the third to first members and the largest plane distribution scale developed in the late stage of the second member.Based on the development of SEDDF depositional system along the source direction,three types of coupling relationships are divided,namely,deltas that are linked downdip to fans,deltas that lack downdip fans and fans that lack updip coeval deltas,with different depositional characteristics and genetic mechanisms.(1)Deltas that are linked downdip to fans:with the development of shelf-edge deltas in the shelf area and deep-water fans in the downdip slope area,and the strong source supply and relative sea level decline are the two key factors which control the development of this type of source-to-sink(S2S).The development of channels on the continental shelf edge is conducive to the formation of this type of S2S system even with weak source supply and high sea level.(2)Deltas that lack downdip fans:with the development of shelf edge deltas in shelf area,while deep water fans are not developed in the downdip slope area.The lack of“sources”and“channels”,and fluid transformation are the three main reasons for the formation of this type of S2S system.(3)Fans that lack updip coeval deltas:with the development of deep-water fans in continental slope area and the absence of updip coeval shelf edge deltas,which is jointly controlled by the coupling of fluid transformation at the shelf edge and the“channels”in the continental slope area.
基金Supported by the National Natural Science Foundation of China(Nos.42302155,42072169,42072235)the Natural Science Foundation of Shandong Province(No.ZR2023QD016)+2 种基金the China Postdoctoral Science Foundation(No.2022M713461)the Qingdao Postdoctoral Application Research Funds(No.QDBSH20220202067)the Fundamental Research Funds for the Central Universities(No.22CX06005A)。
文摘A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon accumulation conditions,the geometry,kinematics,and reservoir control of a large synthetic overlapping transfer zone in the south of the Wenchang A subsag in the Zhujiang(Pearl)River Mouth basin were investigated.Results indicate that the development and evolution of the transfer zone was controlled by the interaction between pre-existing faults and regional stress transformation.The intense rifting of the main faults of the transfer zone controlled the development of source rocks and faultcontrolled slope break paleogeomorphology.The strike-slip overprint since the Oligocene is conducive to the formation of a large-scale fault-anticline trap,and the secondary faults in the transfer zone contribute to the hydrocarbon transportation.The conjugate intersection area of the NE-and NW-trending faults offers more opportunity for hydrocarbon migration and accumulation.
基金The National Natural Science Foundation of China under contract No.42276066the Key Research and Development Program(International Science and Technology Cooperation Development Program)of Hainan Province under contract No.GHYF2022009the Youth Innovation Promotion Association of CAS under contract No.2018401.
文摘Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift normal fault through detailed seismic interpretation and fault modeling.A total of 289 post-rift normal faults were identified in the study area and can be classified into four types:(1)isolated normal faults above the carbonate platform;(2)isolated normal faults cutting through the carbonate platform;(3)conjugate normal faults,and(4)connecting normal faults.Throw distribution analysis on the fault planes show that the vertical throw profiles of most normal fault exhibit flat-topped profiles.Isolated normal faults above the carbonate platform exhibit roughly concentric ellipses with maximum throw zones in the central section whereas the normal faults cutting through the carbonate platform miss the lowermost section due to the chaotic seismic reflections in the interior of the carbonate platform.The vertical throws of conjugate normal faults anomalously decrease toward their intersection region on the fault plane whereas the connecting normal faults present two maximum throw zones in the central section of the fault plane.According to the symmetric elliptical distribution model of fault throw,an estimation was made indicating that normal faults cutting through the carbonate platform extended downward between-1308 s and-1780 s(two-way travel time)in depth and may not penetrate the entire Liuhua carbonate platform.Moreover,it is observed that the distribution of karst caves on the top of the carbonate platform disaccord with those of hydrocarbon reservoirs and the post-rift normal faults cutting through the carbonate platform in the study area.We propose that these karst caves formed most probably by corrosive fluids derived from magmatic activities during the Dongsha event,rather than pore waters or hydrocarbons.
文摘Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells(PBMCs)infected with enterovirus 71(EV-A71)which were derived from patients with HFMD of different severities and at different stages.A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates.Subsequently,ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes,specifically,BST2,IRF7,IFI16,TRIM21,MX1,and DDX58.Results:Compared with those at the recovery stage,the expression levels of BST2(P=0.027),IFI16(P=0.016),MX1(P=0.046)and DDX58(P=0.008)in the acute stage of infection were significantly upregulated,while no significant difference in the expression levels of IRF7(P=0.495)and TRIM21(P=0.071)was found between different stages of the disease.The expression levels of BST2,IRF7,IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens,and BST2,IRF7,IFI16 and MX1 expression levels were significantly lower in coxsackie B virus(COXB)positive patients than the negative patients.Expression levels of one or more of BST2,IRF7,IFI16,TRIM21,MX1 and DDX58 genes were correlated with PCT levels,various white blood cell counts,and serum antibody levels that reflect disease course of HFMD.Aspartate aminotransferase was correlated with BST2,MX1 and DDX58 expression levels.Conclusions:PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD.
文摘Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient sampling, 60 patients with mouth-opening difficulty after treatment for head and neck cancer admitted to the Oncology Department from February 2022 to October 2023 were selected for a 2-week exercise and nursing program. The patients were divided into a control group (February 1, 2022 to November 30, 2022) and an observation group (December 1, 2022 to October 31, 2023), with 30 patients in each group. The control group underwent routine mouth-opening functional exercises combined with cork for oral support training, while the observation group underwent routine mouth-opening functional exercises combined with oral massage and mouth-opening training sticks for oral support training. The mouth-opening degree, mouth-opening difficulty level, comfort level, compliance, and quality of life were observed in both groups. Results: Before the intervention, there were no statistically significant differences in mouth-opening degree and mouth-opening difficulty level between the two groups (P > 0.05). After the intervention, the mouth-opening degree, mouth-opening difficulty level, oral comfort level, compliance, and QLICP-HN scores in the observation group were all better than those in the control group, with statistically significant differences (P < 0.05). Conclusion: The combination of mouth-opening training sticks and oral massage with mouth-opening functional exercises can effectively improve the degree of mouth-opening difficulty, enhance oral comfort, increase compliance with mouth-opening exercises, and improve quality of life.
基金Nivedita Chakraborty and Anudeb Mandal acknowledge UGC and CSIR respectively for their financial support
文摘Mixed siliciclastic-carbonate rocks constitute the Upper Cretaceous Garuda- mangalam Sandstone Formation, Ariyalur (India), and offer an opportunity to look into the broad spectrum of mixing of compositionally and genetically different components. The palaeogeographic reconstruction indicates that deposition in the nearshore zone differed strongly in energy and active processes operatives due to the presence of a shore-parallel river-mouth bar. The western wing of the Mississippi bird-foot delta is considered to be a present-day analogon. Facies analysis in combination with petrography clearly shows the variability in palaeoenvironmental characteristics, both biogenic and non-biogenic. It also indicates dia- genetic uptake of carbonate that filled empty spaces and actively replaced original compo- nents. Chemical staining followed by limited application of cathodoluminescence and energy dispersive X-ray analysis (EDAX) hint at intricacies in mixing arising from the compositional variations in the carbonate components. A model of siliciclastic-carbonate sediment mixing, including both the depositional and diagenetic developments, is presented; it is aimed at generating a better overview of, and a deeper insight into, the physical and chemical mechanisms involved.
基金funded by the National Natural Science Foundation of China(Nos.41776052,41476031)the China-ASEAN Maritime Cooperation Fund,the National Programme on Global Change and Air-Sea Interaction(No.GASI-GEOGE-03)the Open Research Fund of State Key Laboratory of Estuarine and Coastal Research(No.SKLEC-KF201506)
文摘River-mouth bar is a fundamental element to constitute a delta system, and its internal facies architectures are vital to reconstruct delta evolution history and study high-resolution sequence stratigraphy. Changxing Island is a representative mouth-bar complex in the modern Changjiang Delta. Its vertical stacked strata produced by delta progradation were first studied by detailed facies analysis of core CX03, and then a general facies architecture and evolution history of mouth-bar complexes in the modern Changjiang Delta were discussed through a comparative study of 5 different cores and historical charts. A progradational deltaic sequence has generally a similar internal facies architecture, composed of massive muddy deposits of prodelta and delta-front slope facies at the bottom, sand-dominated deposits of delta-front platform, inter-bar channel and mouth-bar facies at the middle, and heterolithic deposits of tidal flat and saltmarsh facies at the top. There is a significant time lag for the initial formation of channel-mouth bar systems between Chongming and Jiuduansha Islands. Compared with subtidal flats, sedimentation rates on intertidal flats were highly accelerated, potentially resulting from weakening wave and tidal strength over extensive intertidal flats and increasing human embankment activities in the last few centuries. These findings provide some new clues to understand modern and ancient mouth-bar evolution and resultant sequence strata in tide-dominated deltas.
文摘Almost at every mouth of the tributary creek of the Three Gorges inthe Gezhouba Reservoir, the alluvial fans or alluvial cones formed the rapids, whichare called rapids of creek mouth--bar. They are composed of pebble bed load with d>20 mm. Based upon geological and geomorphological investigations, the authors detect that the pebble bed load at the Gezhouba Re3ervoir comes from two sources,namely, the region mainly with limestone and The region mainly with pyrolith, andtheir converging area is within the Gezhouba Project. percentages of the pebble bedload are calculated. The calculated result stows that, 65% of pebble bed load comesfrom the region mainly with limestone, and 93 % of the rapids of creek mouth--bar isdistributed in the region mainly with limestone. Considering the water level fluctuations, the rapids of creek mouth--bar can be divided into 3 types i. e. flood waterones, medium water ones and low water ones. Due to the rise of water level afterbuilding the Gezhouba Reservoir, the navigable channels in the area of rapids ofcreek mouth--bar within varied back water region are improved, taking up 50% of all12 rapids. But the rapids of creek mouth--bar in the tail region where the water levelrises less than 1 m, the conditions of navigable channels are little improved and evendeterioration, because of the continuous supply of pebble bed load. Therefore, thoserapids must be regulated before the completion of the Three Gorges Project.
基金This study is financially supported by the National Natural Science Foundation of China(42072181).
文摘The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evolution and for unraveling its poorly studied source-rock maturation history.Our investigations in this study are based on apatite fission-track(AFT)thermochronology analysis of 12 cutting samples from 4 boreholes.Both AFT ages and length data suggested that the PRMB has experienced quite complicated thermal evolution.Thermal history modeling results unraveled four successive events of heating separated by three stages of cooling since the early Middle Eocene.The cooling events occurred approximately in the Late Eocene,early Oligocene,and the Late Miocene,possibly attributed to the Zhuqiong II Event,Nanhai Event,and Dongsha Event,respectively.The erosion amount during the first cooling stage is roughly estimated to be about 455-712 m,with an erosion rate of 0.08-0.12 mm/a.The second erosion-driven cooling is stronger than the first one,with an erosion amount of about 747-814 m and an erosion rate between about 0.13-0.21 mm/a.The erosion amount calculated related to the third cooling event varies from 800 m to 3419 m,which is speculative due to the possible influence of the magmatic activity.
文摘The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The parameters we use in mathematical models are usually classical mathematical parameters,called crisp parameters,which are taken for granted.But any biological or physical phenomenon is best explained by uncertainty.To represent a realistic situation in any mathematical model,fuzzy parameters can be very useful.Many articles have been published on how to control and prevent HFMD from the perspective of public health and statistical modeling.However,few works use fuzzy theory in building models to simulateHFMDdynamics.In this context,we examined anHFMD model with fuzzy parameters.A Non Standard Finite Difference(NSFD)scheme is developed to solve the model.The developed technique retains essential properties such as positivity and dynamic consistency.Numerical simulations are presented to support the analytical results.The convergence and consistency of the proposed method are also discussed.The proposed method converges unconditionally while the many classical methods in the literature do not possess this property.In this regard,our proposed method can be considered as a reliable tool for studying the dynamics of HFMD.
基金Supported by the Natural Science Foundation of Heilongjiang Province(LH2021C032)。
文摘EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.
文摘Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 were selected for this study.The patients were divided into two groups of thirty cases each using the randomized numerical table method.The observation underwent psychological intervention and mindfulness meditation training,while the control group only received symptomatic care.The condition of the patients of both groups was observed and compared.Results:Upon receiving treatment,the patients in the observation group had lower Hamilton Anxiety(HAM-A)scores,and Hamilton Depression(HAMD)scores compared to the control group(P<0.05).The visual analog scale(VAS)scores of the observation group were also lower than those of the control group(P<0.05).Moreover,the efficacy of the nursing intervention in the observation group was higher than that of the control group(P<0.05).Conclusion:Psychological intervention and mindfulness meditation training can effectively improve the clinical symptoms of patients with burning mouth syndrome.Therefore,this treatment method should be popularized.