The porous scaffold of the sol-gel derived bioactive glass （BG） in the system CaO-P2O5-SiO2 was treated with the type Ⅰ collagen solution. The pore walls of the scaffold were covered by the collagenous network. The...The porous scaffold of the sol-gel derived bioactive glass （BG） in the system CaO-P2O5-SiO2 was treated with the type Ⅰ collagen solution. The pore walls of the scaffold were covered by the collagenous network. The in vitro mineralization behavior of the sol- gel derived bioglassl collegen composite porous scaffold was investigated by immersion in supersaturated calcification solution （ SCS ） at 37℃ for different times, XRD , FTIR, SEM/ EDAX techniques were applied to analyze the crystalline phases, morphology and composition of the minerals formed on the pore walls of the scaffold. It was found that with increasing of immersion time, the morphology of reaction products on the pore walls changed from the spherical particles of calcium phosphate to the flake-like HCA crystals.展开更多
The purposes of this study were to construct a novel tissue engineered bone composed of 3Dprinted bioactive glass block/chitosan nanoparticles(BD/CSn)composites loaded with Nel-like Type I molecular-1 DNA(pDNA-NELL1)a...The purposes of this study were to construct a novel tissue engineered bone composed of 3Dprinted bioactive glass block/chitosan nanoparticles(BD/CSn)composites loaded with Nel-like Type I molecular-1 DNA(pDNA-NELL1)and/or bone marrow mesenchymal stem cells(BMSCs),and study their osteogenic activities by repairing bone defects in rhesus monkeys.CSn with NELL1 gene plasmid and rhesus monkey BMSCs were composited with a BD scaffold to prepare the tissue-engineered bone.Four adult female rhesus monkeys with 10-to 12-years old and 5-7 kg in weight were used in animal experiments.The first and second premolar teeth from four regions of each monkey were removed to form bone defects with size of 10×10×5 mm,which were then implanted with above-mentioned tissue engineered bone.At 12 weeks after the implantation,gross observations,X-ray and micro-CT observations revealed that the new bone was extremely close to normal bone in mass,density,hardness,and structure.The bony cortex was smooth and closely connected to the surrounding normal bone.Histological observations revealed moderate inflammation in the repair area,and the new bone tissues were similar to normal ones.In conclusion,tissue engineered bone of this study exhibited good osteoconductivity for promoting the formation of new alveolar bone tissue,and NELL1 gene played a promotional role in bone regeneration.展开更多
采用碱性蛋白酶对猪皮胶原进行酶解得到具有抗冻活性的复合产物,考察了该复合物对冰淇淋浆料黏度、膨胀率、微观结构、冰晶生长、重结晶及Tg’的影响。结果表明,猪皮胶原酶解复合物具有明显改善冰淇淋品质的效果,当酶解复合物添加浓度为...采用碱性蛋白酶对猪皮胶原进行酶解得到具有抗冻活性的复合产物,考察了该复合物对冰淇淋浆料黏度、膨胀率、微观结构、冰晶生长、重结晶及Tg’的影响。结果表明,猪皮胶原酶解复合物具有明显改善冰淇淋品质的效果,当酶解复合物添加浓度为0.5%时,冰淇淋的黏度和膨胀率达到最大,分别为34.5 m Pa·s和26.87%,并表现出最大的重结晶抑制活性,冰淇淋的Tg’也达到最大,为-17.64℃。结合感官分析评价,胶原酶解复合物添加浓度为0.3%时冰淇淋口感最佳,其品质也较高。展开更多
文摘The porous scaffold of the sol-gel derived bioactive glass （BG） in the system CaO-P2O5-SiO2 was treated with the type Ⅰ collagen solution. The pore walls of the scaffold were covered by the collagenous network. The in vitro mineralization behavior of the sol- gel derived bioglassl collegen composite porous scaffold was investigated by immersion in supersaturated calcification solution （ SCS ） at 37℃ for different times, XRD , FTIR, SEM/ EDAX techniques were applied to analyze the crystalline phases, morphology and composition of the minerals formed on the pore walls of the scaffold. It was found that with increasing of immersion time, the morphology of reaction products on the pore walls changed from the spherical particles of calcium phosphate to the flake-like HCA crystals.
基金This work is in part supported by China Postdoctoral Science Foundation funded project(No.2013M540787).
文摘The purposes of this study were to construct a novel tissue engineered bone composed of 3Dprinted bioactive glass block/chitosan nanoparticles(BD/CSn)composites loaded with Nel-like Type I molecular-1 DNA(pDNA-NELL1)and/or bone marrow mesenchymal stem cells(BMSCs),and study their osteogenic activities by repairing bone defects in rhesus monkeys.CSn with NELL1 gene plasmid and rhesus monkey BMSCs were composited with a BD scaffold to prepare the tissue-engineered bone.Four adult female rhesus monkeys with 10-to 12-years old and 5-7 kg in weight were used in animal experiments.The first and second premolar teeth from four regions of each monkey were removed to form bone defects with size of 10×10×5 mm,which were then implanted with above-mentioned tissue engineered bone.At 12 weeks after the implantation,gross observations,X-ray and micro-CT observations revealed that the new bone was extremely close to normal bone in mass,density,hardness,and structure.The bony cortex was smooth and closely connected to the surrounding normal bone.Histological observations revealed moderate inflammation in the repair area,and the new bone tissues were similar to normal ones.In conclusion,tissue engineered bone of this study exhibited good osteoconductivity for promoting the formation of new alveolar bone tissue,and NELL1 gene played a promotional role in bone regeneration.
文摘采用碱性蛋白酶对猪皮胶原进行酶解得到具有抗冻活性的复合产物,考察了该复合物对冰淇淋浆料黏度、膨胀率、微观结构、冰晶生长、重结晶及Tg’的影响。结果表明,猪皮胶原酶解复合物具有明显改善冰淇淋品质的效果,当酶解复合物添加浓度为0.5%时,冰淇淋的黏度和膨胀率达到最大,分别为34.5 m Pa·s和26.87%,并表现出最大的重结晶抑制活性,冰淇淋的Tg’也达到最大,为-17.64℃。结合感官分析评价,胶原酶解复合物添加浓度为0.3%时冰淇淋口感最佳,其品质也较高。
