Most Rhododendron species are ornamental flowering species widely distributed in Asia,North America,and West Europe.Rhododendron fortunei,one of the endemic Rhododendron species in China,has beautiful flowers with bri...Most Rhododendron species are ornamental flowering species widely distributed in Asia,North America,and West Europe.Rhododendron fortunei,one of the endemic Rhododendron species in China,has beautiful flowers with bright colors and is being exploited to meet the needs of the flower market.Polyploid plants usually show superiority in growth,disease resistance,and adaption over their diploid relatives.Here,we report the first case of polyploid induction in R.fortunei.In order to induce polyploidy in R.fortunei,germinating seeds were treated with different concentrations of oryzalin for 16 h.By evaluating ploidy level with flow cytometry,a total of 34 polyploid R.fortunei lines,including 27 tetraploid lines and seven octoploid lines,were obtained.A comparison of treatments indicated that 7.5 mg L^-1 oryzalin was the optimal concentration for polyploid induction in seeds of R.fortunei.Compared with diploid plants,tetraploid and octoploid plants exhibited slower growth rates and had thicker and rounder curled leaves with more leaf epidermal hairs.Moreover,larger stomata at lower density were also observed in the leaves of polyploid plants.Chlorophyll contents were also significantly increased in polyploid plants,which leads to a darker green leaf color.Both small and large individuals exhibiting the same characteristics were observed among the obtained tetraploid plants.Overall,our study establishes a feasible method for polyploid induction in R.fortunei,thus providing a basis for breeding new R.fortunei varieties.展开更多
[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wi...[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer, polyploid induction was performed twice on diploid plants by addition method, and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results] Among the 7 polyploidy plants, four materials were tetraploid, the concentration and treatment time of which were 300 mg/L and 7 and 14 d, respectively; and three materials were octaploid, the concentrations and treatment time of which were 300 and 500 mg/L and 14 d, respectively. [ Conclusions] The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects, and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore, colchicine is an polyploid inducer suitable for L. edulis.展开更多
基金supported by the Tree Breeding Research Project of Zhejiang Province,China(2016C02056-12)。
文摘Most Rhododendron species are ornamental flowering species widely distributed in Asia,North America,and West Europe.Rhododendron fortunei,one of the endemic Rhododendron species in China,has beautiful flowers with bright colors and is being exploited to meet the needs of the flower market.Polyploid plants usually show superiority in growth,disease resistance,and adaption over their diploid relatives.Here,we report the first case of polyploid induction in R.fortunei.In order to induce polyploidy in R.fortunei,germinating seeds were treated with different concentrations of oryzalin for 16 h.By evaluating ploidy level with flow cytometry,a total of 34 polyploid R.fortunei lines,including 27 tetraploid lines and seven octoploid lines,were obtained.A comparison of treatments indicated that 7.5 mg L^-1 oryzalin was the optimal concentration for polyploid induction in seeds of R.fortunei.Compared with diploid plants,tetraploid and octoploid plants exhibited slower growth rates and had thicker and rounder curled leaves with more leaf epidermal hairs.Moreover,larger stomata at lower density were also observed in the leaves of polyploid plants.Chlorophyll contents were also significantly increased in polyploid plants,which leads to a darker green leaf color.Both small and large individuals exhibiting the same characteristics were observed among the obtained tetraploid plants.Overall,our study establishes a feasible method for polyploid induction in R.fortunei,thus providing a basis for breeding new R.fortunei varieties.
基金Supported by The Cooperative Project between the Yanbian University,ChinaNational Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
文摘[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer, polyploid induction was performed twice on diploid plants by addition method, and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results] Among the 7 polyploidy plants, four materials were tetraploid, the concentration and treatment time of which were 300 mg/L and 7 and 14 d, respectively; and three materials were octaploid, the concentrations and treatment time of which were 300 and 500 mg/L and 14 d, respectively. [ Conclusions] The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects, and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore, colchicine is an polyploid inducer suitable for L. edulis.
