[Objectives]To isolate and optimize the potassium releasing bacteria from sugarcane rhizosphere soil in Baitu Town,Gaoyao District,Zhaoqing City,Guangdong Province,and then evaluate their potassium-releasing ability,a...[Objectives]To isolate and optimize the potassium releasing bacteria from sugarcane rhizosphere soil in Baitu Town,Gaoyao District,Zhaoqing City,Guangdong Province,and then evaluate their potassium-releasing ability,and optimize the fermentation conditions of the strains with the best potassium-releasing ability,so as to provide a scientific basis for the development and production of potassium releasing bacteria fertilizer.[Methods]Potassium-solubilizing bacteria were isolated from sugarcane rhizosphere soil using potassium-feldspar powder as the potassium source of isolation medium by dilution coating method and plate streaking method.The isolated strains were identified by 16s rRNA sequence analysis.The potassium-releasing ability of each strain was determined by sodium tetraphenylborate(STPB)method,and the strain GK-37 with the optimal potassium-releasing ability was selected.The fermentation conditions of GK-37 strain were further optimized,and the effects of different carbon sources on its growth were mainly investigated.[Results]Seven strains of potassium-solubilizing bacteria were isolated and purified,and identified by 16s rRNA.They belonged to Pseudomonas knackmussii,Pseudomonas,Pseudomonas insulaes and Caballeronia zhejiangensis.Among the tested strains,strain GK-37 had the best potassium-releasing ability,and its potassium-releasing capacity was 26.99 mg/L.By optimizing the fermentation conditions of GK-37 strain,it was found that when the fermentation medium was sucrose as carbon source,the growth of the strain was the best.[Conclusions]In this study,the potassium releasing bacteria were successfully isolated and identified from the rhizosphere soil of sugarcane,and the strain GK-37 had high potassium-releasing ability.Through the optimization of fermentation conditions of GK-37 strain,sucrose was determined as the optimal carbon source.This study is expected to provide a valuable reference for the further development and production of potassium releasing bacteria fertilizer.展开更多
基金Supported by Guangdong Province Rural Science and Technology Commissioner Project(KTP20240693)Zhaoqing University Project(QN202329)+3 种基金Science and Technology Innovation Guidance Project of Zhaoqing(202304038001)Undergraduate Innovation and Entrepreneurship Training Program(202410580011&X202310580120)The Third Batch of Innovation Research Team of Zhaoqing University(05)Quality Engineering and Teaching Reform Project of Zhaoqing University(zlgc202229,zlgc202261).
文摘[Objectives]To isolate and optimize the potassium releasing bacteria from sugarcane rhizosphere soil in Baitu Town,Gaoyao District,Zhaoqing City,Guangdong Province,and then evaluate their potassium-releasing ability,and optimize the fermentation conditions of the strains with the best potassium-releasing ability,so as to provide a scientific basis for the development and production of potassium releasing bacteria fertilizer.[Methods]Potassium-solubilizing bacteria were isolated from sugarcane rhizosphere soil using potassium-feldspar powder as the potassium source of isolation medium by dilution coating method and plate streaking method.The isolated strains were identified by 16s rRNA sequence analysis.The potassium-releasing ability of each strain was determined by sodium tetraphenylborate(STPB)method,and the strain GK-37 with the optimal potassium-releasing ability was selected.The fermentation conditions of GK-37 strain were further optimized,and the effects of different carbon sources on its growth were mainly investigated.[Results]Seven strains of potassium-solubilizing bacteria were isolated and purified,and identified by 16s rRNA.They belonged to Pseudomonas knackmussii,Pseudomonas,Pseudomonas insulaes and Caballeronia zhejiangensis.Among the tested strains,strain GK-37 had the best potassium-releasing ability,and its potassium-releasing capacity was 26.99 mg/L.By optimizing the fermentation conditions of GK-37 strain,it was found that when the fermentation medium was sucrose as carbon source,the growth of the strain was the best.[Conclusions]In this study,the potassium releasing bacteria were successfully isolated and identified from the rhizosphere soil of sugarcane,and the strain GK-37 had high potassium-releasing ability.Through the optimization of fermentation conditions of GK-37 strain,sucrose was determined as the optimal carbon source.This study is expected to provide a valuable reference for the further development and production of potassium releasing bacteria fertilizer.
