MEAMI(Middle East-Asia Minor 1."B"biotype)anpl MED(Mediterranean,"Q"biotype)are the two most destructive ery pie species of the Bemisia tabaci complex on the planet.Our previous studies have shown ...MEAMI(Middle East-Asia Minor 1."B"biotype)anpl MED(Mediterranean,"Q"biotype)are the two most destructive ery pie species of the Bemisia tabaci complex on the planet.Our previous studies have shown that MEAMI outcompets MED on cabbage;the underlying mechanism is unknown.In the Brassicaceae family,the glucosinolate-myrosinase defense system plays a crucial role im deterring feeding,inhibiting growth,and eausing acute toxicity against a wide range of generalist herbivores.In the present study.we first compared the surv ival of MEAMI and MED exposed to sinigin(a glucosinolate)and myosinase(an enzyme that degrades glucosinolates);we found that survival of both species was high in response 10 sinigrin alone bul was near zer0 in response to sinigrin+myrosinase.We then used electropenetrography(lectrical penetration graphs,EPG)to assess the feeding bchaviors of MEAMI and MED whiteflies on cabbage.The EPG results revealed that the me an dunution of each potential drop(pd,indicating an intreellular puncture)was subsantialy longer for MED than MEAMI on eabbage,indicating that the exposure to the toxic hydrolysates of glucosinolate and myrosinase is greater for MED than for MEAMI.We therefore conclude that dffreces in penetrating bchaviors may help explain the dfferent fiects of cabbuge on MEAMI and MED whitefly species.展开更多
基金the National Key R&D Program of China(2017YFD0200400)National Natural Science Foundation of China(31371941,31572012,31772171,31601637)+1 种基金the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables,the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)the Natural Science Fund of Tianjin(17JCZDJC33700).
文摘MEAMI(Middle East-Asia Minor 1."B"biotype)anpl MED(Mediterranean,"Q"biotype)are the two most destructive ery pie species of the Bemisia tabaci complex on the planet.Our previous studies have shown that MEAMI outcompets MED on cabbage;the underlying mechanism is unknown.In the Brassicaceae family,the glucosinolate-myrosinase defense system plays a crucial role im deterring feeding,inhibiting growth,and eausing acute toxicity against a wide range of generalist herbivores.In the present study.we first compared the surv ival of MEAMI and MED exposed to sinigin(a glucosinolate)and myosinase(an enzyme that degrades glucosinolates);we found that survival of both species was high in response 10 sinigrin alone bul was near zer0 in response to sinigrin+myrosinase.We then used electropenetrography(lectrical penetration graphs,EPG)to assess the feeding bchaviors of MEAMI and MED whiteflies on cabbage.The EPG results revealed that the me an dunution of each potential drop(pd,indicating an intreellular puncture)was subsantialy longer for MED than MEAMI on eabbage,indicating that the exposure to the toxic hydrolysates of glucosinolate and myrosinase is greater for MED than for MEAMI.We therefore conclude that dffreces in penetrating bchaviors may help explain the dfferent fiects of cabbuge on MEAMI and MED whitefly species.
