Effects of V2O5 on Proliferation and Differentiation of Limb Bud Cells of Rat

The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells.

微小RNA-199a-5p保护大鼠脑缺血后血-脑屏障完整性

目的探讨微小RNA(miR)-199a-5p保护脑缺血后血-脑屏障(BBB)完整性的机制。方法通过SPF级成年雄性SD大鼠建立永久性大脑中动脉闭塞(MCAO)模型,48只大鼠随机分为假手术组、模型组、MCAO+miR-199a-5p组和MCAO+miR-199a-5p阴性对照组,每组12只。应用Ludmila Belayev 12分评分法评估大鼠的神经行为学表现;采用Evans蓝染色检测脑缺血后BBB的完整性;免疫荧光染色检测脑缺血后细胞凋亡;Western blotting技术检测claudin-5、磷脂酰肌醇-3激酶调节亚基2(PIK3R2)、p-Akt、Akt和血管内皮生长因子(VEGF)-A的蛋白表达;利用Real-time PCR检测脑缺血半暗带、梗死区miR-199a-5p、claudin-5及VEGF-A表达水平。结果MiR-199a-5p mimic干预增强MCAO大鼠本体感觉和运动能力。MiR-199a-5p降低脑缺血后PIK3R2的表达,激活Akt信号通路,并增加缺血半暗带中claudin-5和VEGF-A的表达。此外,miR-199a-5p减轻了脑缺血后的炎症。MiR-199a-5p降低脑缺血后BBB渗透性,减少神经细胞凋亡。结论MiR-199a-5p可降低缺血性脑卒中后PIK3R2的表达,激活Akt信号通路,降低炎性细胞因子的表达,减轻缺血性脑卒中对BBB的破坏。

5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina

AIM: To investigate the roles of a DNA methyltransferase(DNMT) inhibitor 5-aza-2'-deoxycytidine(5-aza-dC) in the regulation of antioxidant enzymes in diabetic retinopathy(DR) models. METHODS: DNMTs expressions and activity, and changes of two key antioxidant enzymes in DR, MnSOD(encoded by SOD2 gene) and glutathione S-transferase theta 1(GSTT1), were quantified in the isolated human retinal endothelial cells(HRECs) exposed to high glucose(HG) with or without 5-aza-dC treatment. The downstream exacerbating factors including vascular endothelial growth factor(VEGF), intercellular adhesion molecule 1(ICAM-1) and matrix metalloproteinase 2(MMP2), which are implicated in the pathogenesis of DR and closely related to oxidative stress were also analyzed. The key parameters were confirmed in the retina from streptozotocin(STZ) diabetic rats. RESULTS: DNMTs expression and DNMT activity was induced in HRECs exposed to HG. Hyperglycemia decreased MnSOD and GSTT1 expression. 5-aza-dC administration effectively suppressed DNMTs expression and activity and reversed the MnSOD and GSTT1 expression under HG condition. VEGF, ICAM-1 and MMP2 induced by HG were also suppressed by 5-aza-dC treatment. Similar results were observed in the retina from STZ diabetic rats. CONCLUSION: Our findings suggest that DNA methylation may serves as one of the mechanisms of antioxidant defense system disruption in DR progression. Modulation of DNA methylation using pharmaceutic means such as DNMT inhibitors could help maintain redox homeostasis and prevent further progression of DR.

Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats

AIM： To determine the effect of chemotherapy on wound healing by giving early preoperative 5-fluorouracil （5-FU） to rats with colonic anastomoses.METHODS： Sixty Albino-Wistar male rats （median weight, 235 g） were used in this study. The rots were fed with standard laboratory food and given tap water ad libitum. The animals were divided into three groups： Group 1： Control group （chemotherapy was not administered）, Group 2： Intraperitoneally （IP） administered 5-FU group （chemotherapy was administered IP to animals at a dose of 20 mg/kg daily during the 5 d preceeding surgery）, Group 3： Intravenously （IV） administered 5-FU group. Chemotherapy was administered v/a the penil vein, using the same dosing scheme and duration as the second group. After a 3-d rest to minimize the side effects of chemotherapy, both groups underwent surgery. One centimeter of colon was resected 2 cm proximally from the peritoneal reflection, then sutured intermittently and subsequently end-to-end anastomosed. In each group, half the animals were given anaesthesia on the 3rd postoperative （PO） day and the other half on the 7th PO day, for in vivo analytic procedures. The abdominal incisions in the rats were dissected, all the new and old anastomotic segments were clearly seen and bursting pressures of each anastomotic segment, tissue hydroxyproline levels and DNA content were determined to assess the histologic tissue repair process. RESULTS： When the IV group was compared with the IP group, bursting pressures of the anastomotic segments on the 3rd and 7th PO days, were found to be significantly decreased, hydroxyproline levels at the anastomotic segment on the 7th PO day were significantly decreased （P 〈 0.01）. CONCLUSION： In this study, we conclude that early preoperative 5-FU, administered IV, negatively affects wound healing. However, IP administered 5-FU does not negatively affect wound healing.

Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma

BACKGROUND: OCI-5, the rat homologene of human glypican 3 ( GPC3), is confirmed upregulated in hepatocellular carcinoma (HCC). The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC. METHODS: Male Sprague-Dawley rats were given diethyl-nitrosamine ( DENA) to induce HCC. Three DENA-induced rats and one control rat were sacrificed every week for 18 weeks during the development of HCC. Tissues specimens were snap-frozen in liquid nitrogen and total RNA was isolated. Sk-Hepl cells were treated with DENA at different concentrations. The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method. RESULTS: OCI-5 was not expressed in normal rat liver tissues. When HCC occurred and aggravated, OCI-5 expression was gradually elevated to a very high level. GPC3 was not expressed in the DENA-treated Sk-Hepl cells. CONCLUSIONS: OCI-5 was not expressed in normal rat liver tissues but in rat HCC tissues. High-expression of OCI-5 in DENA-induced rat HCC model was the gene expression change of HCC not the DENA-induced gene expression. The expression level of OCI-5 was not only elevated in rat HCC but also gradually along the occurrence and progression of HCC, indicating that GPC3 might serve as a sensitive marker of early stage HCC.

Temporal and spatial distribution of metabotropic glutamate receptor 5 during development in the rat cortex and hippocampus

Metabotropic glutamate receptor 5 （mGluR5） is expressed by neurons in zones of active neurogenesis and is involved in the development of neural stem cells in vivo and in vitro. We examined the expression of mGluR5 in the cortex and hippocampus of rats during various prenatal and postnatal periods using immunohistochemistry. During prenatal development, mGluR5 was pdmadly localized to neuronal somas in the forebrain. During early postnatal periods, the receptor was mainly present on somas in the cortex, mGluR5 immunostaining was visible in apical dendrites and in the neuropil of neurons and persisted throughout postnatal development. During this period, pyramidal neurons were strongly labeled for the receptor. In the hippocampal CA1 region, mGluR5 immunoreactivity was more intense in the stratum oriens, stratum radiatum, and lacunosum moleculare at P0, P5 and P10 relative to P60. mGluR5 expression increased significantly in the molecular layer and decreased significantly in the granule cell layer of the dentate gyrus at P5, P10 and P60 in comparison with P0. Furthermore, some mGluR5-positive cells were also bromodeoxyuridine- or NeuroD-positive in the dentate gyrus at P14. These results demonstrate that mGluR5 has a differential expression pattern in the cortex and hippocampus during early growth, suggesting a role for this receptor in the control of domain specific brain developmental events.

The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats

Objective: To investigate the effects of quercetin(Q) and rutin on 5-fluorouracil(5-FU)-induced hepatotoxicity.Methods: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU(50 mg/kg) in the 18th day. The group rutin 100 was rutin(100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU(50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin(100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized rats.Results: The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU groups.Conclusions: In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity.

Effect of the pineal gland on 5-hydroxytryptamine and γ-aminobutyric acid secretion in the hippocampus of male rats during the summer and winter

Objective:The present study aimed to investigate the effect of seasonal variation on neurotransmitter release in the hippocampus of normal rats and rats with pineal excision.Methods:Two time points,the summer and winter solstice,which are the longest and shortest days of the year,respectively,were selected.Male Spraguee Dawley rats that underwent a sham operation without pineal excision were included as a control group.The concentrations of 5-hydroxytryptamine(5-HT)andγ-aminobutyric acid(GABA)were determined by radioimmunoassays and enzyme-linked immunosorbent assays,respectively.Results:In the winter,the 5-HT and GABA levels in normal rats exhibited a significant difference compared with those in the operation group(P<.01).A difference was also noted in GABA levels between the normal group and the sham operation group(P<.05).The concentrations of 5-HT and GABA in the hippocampal tissues of the normal group exhibited a seasonal rhythm consisting of elevation during the summer and reduction during the winter(P<.01),while the GABA levels in the sham operation group exhibited a significant difference,with elevation during the summer and reduction during the winter(P<.01).In the operation group,GABA showed the same trend(P<.01).Conclusion:The seasonal rhythm of neurotransmitter secretion by the hippocampus(5-HT and GABA)consisted of elevation during the summer and reduction during the winter.During the winter,the pineal gland exhibited a reverse regulatory effect on the secretion of 5-HT and GABA in the hippocampus,and it exhibited seasonal selectivity with regard to the regulation of 5-HT.

PNPLA5-knockout rats induced by CRISPR/Cas9 exhibit abnormal bleeding and lipid level

Patatin-like phospholipase domain containing 5 （PNPLA5） is a neotype neutral lipase with dual activity of anabolism and catabolism in vitro and in vivo, which has a low mRNA expression level in humans and mice. PNPLA5, which is localized to lipid droplets and required for efficient autophagy by optimal initiation, has been speculated to possess triglyceride hydro- lase activity, and has been associated with low density lipoprotein cholesterol （LDL-C）. Above all, PNPLA5 is a relatively new gene, which is reported less about its biological function research, especially the function research in the rats is still blank. In this study, we examined the spatiotemporal expression profile of PNPLA5 and found that it was expressed at low levels in most organs of Sprague Dawley （SD） rats, but was present at very high levels in the skin and testes. To furth.er determine the biological function of PNPLA5 in mammals, we generated PNPLA5-knockout SD rats using the clustered regularly-interspaced short palindromic repeats （CRISPR）/Cas9 system. PNPLA5-null rats were viable, but showed a variety of phenotypic abnormalities, such as abnormal bleeding, and varied hematobiochemical parameters including increased serum total cholesterol （TC）, tdglycerides and high density lipoprotein cholesterol （HDL-C） level, and reduced LDL-C level, compared with wild-type control rats. These data are consistent with an important role for PNPLA5 in lipid metabolism, provJdJng a new target gene and animal model for treatment of cardiovascular diseases in the future.

四神丸对腹泻型肠易激综合征大鼠5-HT信号通路表达的影响

目的通过研究四神丸对腹泻型肠易激综合征(IBS-D)模型大鼠5-羟色胺(5-HT)信号通路表达的影响,进一步揭示其治疗IBS-D的内脏敏感机制。方法SD大鼠32只,随机分为正常组、模型组、四神丸组和得舒特组,采用番泻叶灌胃联合避水应激法制备IBS-D大鼠模型,给药14d后进行Bristol粪便性状量表评分,依据腹壁撤退反射评定内脏疼痛阈值,ELISA法检测血清5-HT、SERT含量,免疫组化法检测结肠组织5-HT的阳性表达,Westernblotting法检测结肠组织5-HT4R的蛋白表达。结果与正常组比较,模型组大鼠Bristol粪便性状量表评分升高,内脏疼痛阈值降低,血清中5-HT含量增加、SERT含量减少,结肠组织中5-HT的阳性表达升高、5-HT4R的蛋白表达降低(P<0.05或P<0.01);与模型组比较,四神丸组大鼠Bristol粪便性状量表评分降低、内脏疼痛阈值升高,血清5-HT含量减少,SERT含量增加,结肠组织5-HT的阳性表达降低、5-HT4R的蛋白表达升高(P<0.05或P<0.01),且对5-HT、5-HT4R表达的影响四神丸组优于得舒特组(P<0.05)。结论四神丸治疗IBS-D的内脏敏感机制可能与调节5-HT信号通路的表达有关。

Changes in 5α-reductase (type 2) gene expression in epididymis of puberty diabetic rats

The changes in 5α-reductase (type 2 ) gene expression in the epi-didymis of puberty diabetic rats were studied by the Northern blot and Dotblot method. Rats were divided into 3 groups: the control group (C), thediabetic group (D), and the diabetic group with insulin treatment (DI).Results: The Northern blot intensity of the caput epididymis in Group D is

THE ABNORMAL EXPRESSION OF CLONED REPEATED SEQUENCE DNA, L5B-4, IN RAT HEPATOMA BERH-2

A repeated sequence DNA fragment, L5B-4, was cloned from the 5 kb BamHI DNA fragments of rat genomic DNA. The expressions of the L5B-4 DNA fragment are different in liver and hepatoma cells. The amounts of transcripts in hepatoma cells are lower in nucleus and higher in cytoplasm, especially in polysomal RNA, as compared with that in liver cells. The alteration shown in polysomal RNA of hepatoma cells seems to be specific. These results are discussed with respect to the possible function of this repeated DNA and its variation in hepatoma cells.

Effect of Repetitive Bleeding on NADH-Cytochrome b_5 Methemoglobin Reductase Activity and Molybdenum Content in Erythrocytes of Rats

Reticulocytosls in rats was induced by repetitive bleeding. Both the in vitro and the in vivo studies showed that the detected reductive speed of methemoglobin of the bleeding group was faster than that of the control group at all time intervals. At the same time, the NADH-cytochrome b5 methemoglobin reductase activity and the molybdenum content in erythrocytes of the bleeding group were significantly increased. Regressional analysis showed that there was a significantly positive correlation between the enzyme activity and the molybdenum content. It is proposed that molybdenum might be required for the enzyme activity

PGC⁃1α、FNDC5蛋白在来曲唑诱导多囊卵巢综合征大鼠卵巢组织中的表达

目的探讨过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、Ⅲ型纤连蛋白域蛋白5(FNDC5)在来曲唑诱导多囊卵巢综合征(PCOS)大鼠卵巢组织中的表达。方法将6周龄雌性SD大鼠随机分为对照组、PCOS组,每组各10只。PCOS组给予来曲唑溶液灌胃建立PCOS模型,对照组给予等体积羧甲基纤维素溶液灌胃,均1天1次,连续28 d。用化学发光法测定血清雄激素(T)、黄体生成素(LH)、卵泡雌激素(FSH)、空腹葡萄糖(FPG),计算LH/FSH;用ELISA法测定空腹血胰岛素(INS),计算胰岛素抵抗指数(HOMA-IR);HE染色后观察卵巢组织病理变化;免疫组化染色法检测卵巢组织PGC-1α、FNDC5蛋白表达。结果两组血清FPG比较差异无统计学意义(P>0.05),PCOS组血清T、LH、LH/FSH、INS、HOMA-IR较对照组高(P均<0.05),血清FSH水平较对照组低(P均<0.05)。对照组大鼠可见不同生长时期的卵泡及新鲜黄体,颗粒细胞整齐紧密排列、层数较多,可达6~8层;PCOS组可见多个未成熟的囊状卵泡及闭锁卵泡,颗粒细胞排列散乱不规则,层数较少。PGC-1α、FNDC5蛋白主要在卵巢组织颗粒细胞和卵丘细胞表达,呈现棕黄色,而卵泡膜细胞少量表达。与对照组比较,PCOS组卵巢组织PGC-1α蛋白表达低,FNDC5蛋白表达高,差异有统计学意义(P均<0.05)。结论PGC-1α在PCOS大鼠卵巢组织中低表达,FNDC5在PCOS大鼠卵巢组织中高表达。

烫伤大鼠肠道防御素-5和相关酶转录表达及与细菌移位关系的探讨

目的 探讨烧伤对大鼠肠道防御素 5 ( RD 5 )及其活化所需的基质金属蛋白酶 Matrilysin表达的影响及与肠道细菌移位的关系。方法 SPF级 Wistar大鼠 32只 ,随机分为对照组 (假烫组 ,n=8)和烫伤组 ( n=2 4 )。烫伤组大鼠均在背上造成 30 %总体表面积 度烫伤。伤后 8、2 4和 72 h切取末端回肠 ,利用逆转录聚合酶链反应 ( RT PCR)方法测定不同时间点 RD 5 m RNA及 Matrilysin m RNA表达变化。伤后 2 4 h同时取肠系膜淋巴结 ( ML N )、肝、脾、肺组织进行细菌培养 ,计算脏器细菌移位频率 ;取回肠观察帕内特细胞形态。结果 RD 5 m RNA表达量在伤后 8h显著升高 ( P<0 .0 5 ) ,伤后 2 4 h降低 ,但仍高于对照组水平 ,而伤后 72 h则降至对照组以下 ;Matrilysin m RNA在伤后 8h和 2 4 h略有增高 ,但伤后 72 h表达显著增强( P<0 .0 5 )。伤后 2 4 h脏器细菌移位频率 ( 5 8.3% )显著高于对照组 ( 8.3% ) ,P<0 .0 1;帕内特细胞无明显形态学改变。结论 大鼠烫伤后早期肠道 RD 5 m RNA及 Matrilysin m RNA表达增强 ,但时相略有不同。

5-HT_2和5-HT_3受体在外周初级感觉神经末梢痛反应和痛调制中的相互作用

目的:探讨5-HT2和5-HT3受体亚型在5-HT引起外周痛反应和痛调制中的相互作用及其机制;方法:在大鼠三叉神经节神经元标本上应用全细胞膜片钳技术记录5-羟色胺激活电流(I5-HT),并结合痛行为实验进行观察。结果:在大多数受检细胞(54/88,61.4%)特别是中、小型细胞外加5-HT可引起一快去敏感的内向电流,此内向电流能被5-HT3受体特异性激动剂2-甲基-5-羟色胺所模拟,被5-HT3受体拮抗剂ICS250-930可逆性阻断,而5-HT2受体激动剂α-甲基-5-羟色胺则有明显增强I5-HT的作用,5-HT1受体激动剂R-(+)-UH301无明显反应。在进一步的整体清醒动物的行为学试验中我们观察到,大鼠后肢掌底皮下注射5-HT(10-5,10-4和10-3mol/L)引起浓度依赖性的痛行为反应,而用5-HT2和5-HT3受体特异性拮抗剂Cyproheptadine和ICS250-930分别阻断相应受体亚型后,5-HT引起的痛行为反应的强度序列为:5-HT>5-HT+ICS>5-HT+Cyp。结论:本文结果提示:5-HT所引起的痛反应中,在初级感觉神经元水平5-HT3受体可能仅起着启始作用,而5-HT2受体则在伤害性信息的维持和调制过程中发挥更大的作用。

睡眠剥夺对大鼠脑5-羟色胺代谢及行为的影响

目的探讨长时间睡眠剥夺（sleepdeprivation，SD）对大鼠下丘脑和脑干5-羟色胺（5-HT）代谢及行为的影响。方法用水上转盘（dish-over-water）睡眠剥夺模型对大鼠分别进行24h、48h、72h睡眠剥夺，观察睡眠剥夺后下丘脑、脑干两脑区5-HT代谢的变化，同时利用旷场试验观察大鼠主动行为的改变。结果睡眠剥夺大鼠的旷场试验得分较非剥夺大鼠高（P＜0.05），但随着时间的延长出现下降趋势;5-HT向5-羟吲哚乙酸（5-HIAA）转化在经过24h睡眠剥夺后显著增高（P＜0.01），但在48h开始下降，72h后出现大幅下降（P＜0.01）。结论长时间睡眠剥夺对大鼠行为的影响是一个由兴奋到抑制的过程，这种变化可能与下丘脑和脑干的5-HT代谢有关。

复方肾华片对5/6肾切除大鼠的疗效观察

目的观察复方肾华片对大鼠残余肾功能的保护作用。方法以安博维为对照,复方肾华片治疗5/6肾切除大鼠12周后,观察血压、尿蛋白、血生化及肾脏病理变化情况。结果复方肾华片能降低5/6肾切除大鼠的血清肌酐、尿素氮(P<0.05),24h尿蛋白排泄(P<0.01),降低血清胆固醇浓度(P<0.05),升高血清白蛋白,减轻肾小球硬化及肾间质纤维化程度,但对大鼠血压没有影响。结论复方肾华片可以通过非血流动力学机制,减轻5/6肾切除大鼠的肾损害,延缓慢性肾功能不全的进展。

针刺太冲和天枢对腹泻型肠易激综合征大鼠5-羟色胺、去甲肾上腺素及降钙素基因相关肽的影响

目的探讨电针不同穴位对腹泻型肠易激综合征(D-IBS)模型大鼠的效应差异。方法采用急慢性应激刺激法制作D-IBS大鼠模型。将实验动物分为对照组、模型组、天枢+足三里组、太冲+足三里组,针刺组分别给予双侧相应穴位电针刺激20 min,隔日1次,共治疗7次。观察各组大鼠体征状况,检测各组大鼠血浆5-羟色胺(5-HT)、去甲肾上腺素(NE)、降钙素基因相关肽(CGRP)的含量。结果与对照组比较,模型组大鼠血浆5-HT、NE含量明显增加,CGRP含量降低(P<0.05);与模型组比较,针刺组大鼠5-HT、NE含量明显降低,CGRP含量升高(P<0.05);两针刺组间比较无明显差异。结论针刺"太冲"和"天枢"可能通过降低模型大鼠血浆5-HT、NE含量,增加CGRP含量达到治疗D-IBS的目的。

电针预处理对慢性应激抑郁模型大鼠海马内5-HT及5-HT转运体表达的影响

目的:观察电针及其预处理对慢性应激抑郁模型(CUMS)大鼠海马中5-羟色胺(5-HT)及5-羟色胺转运体(5-HTT)表达的影响,探讨5-HT、5-HTT在大鼠实验性抑郁症发病过程中的作用机制及电针干预的影响。方法:将60只清洁健康雄性SD大鼠,随机分为空白组、模型组、脉冲电针组、电针预处理组及百忧解组,每组12只。孤养结合CUMS 21天复制抑郁症模型,通过开野试验观察大鼠行为学变化,采用免疫组织化学法检测5-HT、5-HTT在大鼠海马中的表达情况。结果:模型组大鼠开野实验水平运动及垂直运动明显减少,脉冲电针组、电针预处理组及百忧解组均可改善此变化;模型组大鼠海马内5-HT、5-HTT表达均明显降低,脉冲电针组、电针预处理组及百忧解组可逆转此病理变化,在5-HT方面,两组电针治疗及百忧解治疗之间无明显统计学差异;在5-HTT方面,电针预处理有上调趋势,但无统计学意义,较脉冲电针与百忧解组疗效稍逊色。结论:抑郁大鼠的5-HT、5-HTT含量低于正常,电针治疗抑郁症可能通过升高5-HT、5-HTT的含量而发挥抗抑郁作用。电针预处理与脉冲电针治疗抑郁症效果差异不明显。