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Additional far-red light promotes adventitious rooting of doubleroot-cutting grafted watermelon seedlings
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作者 Xue Wu Xiaoyan Zhang +8 位作者 Yaya Wang Cuinan Wu Yudong Sun Yi Zhang Yongran Ji Encai Bao Liru Xia Zhonghua Bian Kai Cao 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第6期1424-1436,共13页
Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of root... Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of rootstock in grafted watermelon(Citrullus lanatus)seedlings.In this study,the effects of different light treatments on root regeneration were determined.This revealed that addition of far-red light(Fr)could significantly expedite root formation in the rootstock.Moreover,the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr,especially for auxin-response proteins(including CmIAA11,CmIAA17,and CmAUX28),Small auxinup RNA genes(including CmSAUR20 and CmSAUR50)and the auxin efflux transporter(CmPIN3).In addition,the expression of Phytochrome Interacting Factor(PIFs),such as CmPIF1,CmPIF3 and CmPIF7,was remarkably increased by Fr.These genes may act together to activate auxinrelated pathways under Fr treatment.Based on the results of HPLC-MS/MS analysis,the concentrations of different auxin-types in adventitious root were significantly influenced by Fr.Furthermore,the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment,which was determined by the acid magenta dyeing experiment.Therefore,all the results suggested that Fr could induce AR formation in rootstocks,which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes.The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings,which will provide a theoretical basis for the speeding development of industrialized seedlings production. 展开更多
关键词 WATERMELON Double-root-cutting grafting rootSTOCK Adventitious root regeneration AUXIN
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Exogenous melatonin improves cotton yield under drought stress by enhancing root development and reducing root damage 被引量:1
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作者 Lingxiao Zhu Hongchun Sun +8 位作者 Ranran Wang Congcong Guo Liantao Liu Yongjiang Zhang Ke Zhang Zhiying Bai Anchang Li Jiehua Zhu Cundong Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第10期3387-3405,共19页
The exogenous application of melatonin by the root drenching method is an effective way to improve crop drought resistance.However,the optimal concentration of melatonin by root drenching and the physiological mechani... The exogenous application of melatonin by the root drenching method is an effective way to improve crop drought resistance.However,the optimal concentration of melatonin by root drenching and the physiological mechanisms underlying melatonin-induced drought tolerance in cotton(Gossypium hirsutum L.)roots remain elusive.This study determined the optimal concentration of melatonin by root drenching and explored the protective effects of melatonin on cotton roots.The results showed that 50μmol L-1 melatonin was optimal and significantly mitigated the inhibitory effect of drought on cotton seedling growth.Exogenous melatonin promoted root development in drought-stressed cotton plants by remarkably increasing the root length,projected area,surface area,volume,diameter,and biomass.Melatonin also mitigated the drought-weakened photosynthetic capacity of cotton and regulated the endogenous hormone contents by regulating the relative expression levels of hormone-synthesis genes under drought stress.Melatonin-treated cotton seedlings maintained optimal enzymatic and non-enzymatic antioxidant capacities,and produced relatively lower levels of reactive oxygen species and malondialdehyde,thus reducing the drought stress damage to cotton roots(such as mitochondrial damage).Moreover,melatonin alleviated the yield and fiber length declines caused by drought stress.Taken together,these findings show that root drenching with exogenous melatonin increases the cotton yield by enhancing root development and reducing the root damage induced by drought stress.In summary,these results provide a foundation for the application of melatonin in the field by the root drenching method. 展开更多
关键词 COTTON DROUGHT MELATONIN root morphology root physiology yield
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生物陶瓷封闭剂nRoot和生物陶瓷牙胶尖的根尖封闭性研究
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作者 王培儒 牛一山 +1 位作者 武万超 韩宇环 《中国美容医学》 CAS 2024年第7期60-64,共5页
目的:体外评价国产新型生物陶瓷根管封闭剂nRoot的根尖封闭性以及应用生物陶瓷根管封闭剂时生物陶瓷牙胶尖的根尖封闭性,为临床材料选择提供理论依据。方法:选取单根管的离体前磨牙,自釉牙骨质界处截去牙冠,随机分为四个实验组和两个对... 目的:体外评价国产新型生物陶瓷根管封闭剂nRoot的根尖封闭性以及应用生物陶瓷根管封闭剂时生物陶瓷牙胶尖的根尖封闭性,为临床材料选择提供理论依据。方法:选取单根管的离体前磨牙,自釉牙骨质界处截去牙冠,随机分为四个实验组和两个对照组。通过逐步深入技术进行根管预备,然后采用Martin TroPe推介的生物陶瓷封闭剂改良热牙胶垂直加压充填技术进行根管充填。A组:nRoot根管封闭剂联合生物陶瓷牙胶尖;B组:nRoot根管封闭剂联合普通牙胶尖;C组:iRoot SP根管封闭剂联合生物陶瓷牙胶尖;D组:iRoot SP根管封闭剂联合普通牙胶尖;E组和F组分别为阳性对照组和阴性对照组。通过染料渗透法和扫描电镜法分别观测各组根尖封闭性情况,染料渗透长度越长,微缝隙越宽,表示根尖封闭性越差。结果:染料渗透实验结果显示,A组(2.455±0.137)mm,B组(2.830±0.148)mm,C组(2.426±0.130)mm,D组(2.774±0.163)mm。经方差分析得出,iRoot SP和nRoot差异无统计学意义(P>0.05);生物陶瓷牙胶尖和普通牙胶尖差异有统计学意义(P<0.05),且生物陶瓷牙胶尖染料渗透平均值低于普通牙胶尖;根管封闭剂与牙胶尖交互效应不显著(P>0.05)。扫描电镜结果显示,各组充填材料与牙体组织结合界面均可见大小不等的微缝隙,其中使用普通牙胶尖的B组和D组中,微缝隙较宽,而使用生物陶瓷牙胶尖的A组和C组中,微缝隙相对较小;B组与D组之间,A组与C组之间,微缝隙宽度均没有明显区别。结论:①国产新型生物陶瓷根管封闭剂nRoot具有与进口生物陶瓷根管封闭剂iRoot SP相似的根尖封闭性;②应用生物陶瓷根管封闭剂时,生物陶瓷牙胶尖的根尖封闭性优于普通牙胶尖;③充填材料中根管封闭剂和牙胶尖对根尖封闭性的影响相互独立,互不干扰。 展开更多
关键词 nroot根管封闭剂 iroot SP根管封闭剂 生物陶瓷牙胶尖 普通牙胶尖 根尖封闭性
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双波长激光联合生物陶瓷材料iRoot SP辅助磨牙根管治疗效果分析
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作者 王冕 姚莉莉 +2 位作者 韩爽 笪海芹 侯颖 《上海口腔医学》 CAS 2024年第4期421-425,共5页
目的:探讨双波长激光联合生物陶瓷材料iRoot SP辅助磨牙根管治疗的效果。方法:选择2020年1月—2023年6月收治的104例磨牙根管治疗患者,按照随机数字表法分为对照组与试验组,每组52例。对照组采用iRoot SP糊剂进行根管充填,试验组以双波... 目的:探讨双波长激光联合生物陶瓷材料iRoot SP辅助磨牙根管治疗的效果。方法:选择2020年1月—2023年6月收治的104例磨牙根管治疗患者,按照随机数字表法分为对照组与试验组,每组52例。对照组采用iRoot SP糊剂进行根管充填,试验组以双波长激光(Nd:YAG和Er:YAG)联合iRoot SP糊剂进行治疗。比较2组总有效率、疼痛程度及并发症,观察治疗前、治疗后6周及12周的血清白细胞介素6(IL-6)、白细胞介素17(IL-17)、肿瘤坏死因子α(TNF-α)、出血指数(bleeding index,BI)、牙周探诊深度(depth of periodontal probing,PD)和临床附着水平(clinical attachment level,CAL)。采用SPSS 22.0软件包对数据进行统计学分析。结果:试验组总有效率显著高于对照组,根管填充后疼痛程度显著低于对照组(P<0.05);试验组治疗后6、12周的IL-6、IL-17、TNF-α、BI、PD和CAL显著低于对照组(P<0.05)。结论:双波长激光联合生物陶瓷材料iRoot SP辅助磨牙根管治疗效果较为理想,有利于减轻牙周炎症和疼痛程度。 展开更多
关键词 双波长激光 生物陶瓷材料iroot SP 磨牙 根管治疗 疼痛 炎性因子
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iRoot SP单尖法根管充填对牙体牙髓病患者的疗效及对疼痛、炎症反应的影响
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作者 刘健 陈思宇 陈乙朴 《中南医学科学杂志》 CAS 2024年第5期857-860,共4页
目的 探讨iRoot SP单尖法根管充填对牙体牙髓病患者的疗效及对疼痛、炎症反应的影响。方法 选取106例牙体牙髓病患者,随机均分为常规组和观察组。常规组采用AHplus根充糊剂填充治疗,观察组采用iRoot SP单尖法根管充填治疗,两组均治疗3... 目的 探讨iRoot SP单尖法根管充填对牙体牙髓病患者的疗效及对疼痛、炎症反应的影响。方法 选取106例牙体牙髓病患者,随机均分为常规组和观察组。常规组采用AHplus根充糊剂填充治疗,观察组采用iRoot SP单尖法根管充填治疗,两组均治疗3个月。比较两组患者根管填充时间、恢复时间、临床疗效、疼痛、炎症反应白细胞介素-6(IL-6)、高敏C反应蛋白(hs-CRP)水平、并发症发生率。结果 观察组患者根管填充时间、恢复时间短于常规组(P<0.05)。观察组总有效率高于常规组(P<0.05)。术后,两组患者IL-6与hs-CRP水平较术前均上升,且观察组低于常规组(P<0.05),观察组疼痛感低于常规组(P<0.05)。观察组并发症发生率低于常规组(P<0.05)。结论 iRoot SP单尖法根管充填治疗牙体牙髓病,能够缩短患者填充时间、恢复时间,提高疗效,降低炎症反应,缓解患者的疼痛感,降低并发症发生率,具有一定的推广价值。 展开更多
关键词 iroot SP单尖法根管充填 AHplus根充糊剂填充 牙体牙髓病 疼痛 炎症反应
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牙体牙髓病根管治疗应用iRoot SP冷侧法的效果
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作者 王珺 吉凯 +2 位作者 高波 刘琳 杨婷 《武警医学》 CAS 2024年第3期226-229,共4页
目的探讨牙体牙髓病根管治疗应用iRoot SP冷侧法的效果。方法筛选2018-06至2020-06在解放军总医院京南医疗区就诊的牙体牙髓病患者100例,随机分为对照组和试验组,每组50例,均进行根管治疗,其中对照组充填根管采用热牙胶垂直加压法,试验... 目的探讨牙体牙髓病根管治疗应用iRoot SP冷侧法的效果。方法筛选2018-06至2020-06在解放军总医院京南医疗区就诊的牙体牙髓病患者100例,随机分为对照组和试验组,每组50例,均进行根管治疗,其中对照组充填根管采用热牙胶垂直加压法,试验组采用iRoot SP冷侧法。比较两组根管充填效果、临床总有效率、疼痛视觉模拟评分(VAS)及咀嚼功能。结果两组治疗后根管充填效果和临床总有效率比较,差异无统计学意义(P>0.05);试验组治疗后1周[(4.13±0.79)vs.(5.11±0.98)]及1个月[(2.45±0.37)vs.(3.11±0.67)]VAS评分低于对照组,治疗后咬合力[(131.25±11.21)lbs vs.(120.34±14.09)lbs]及咀嚼效率[(90.12±4.13)%vs.(78.98±7.45)%]均优于对照组,差异均有统计学意义(P<0.05)。结论根管治疗牙体牙髓病中应用iRoot SP冷侧法的充填效果和疗效与热牙胶垂直加压法基本相同,但iRoot SP冷侧法在减轻患者疼痛,改善咀嚼功能方面更具优势。 展开更多
关键词 牙体牙髓病 根管治疗 iroot SP冷侧法 垂直加压法 咀嚼 疼痛
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Evolution history dominantly regulates fine root lifespan in tree species across the world
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作者 Xingzhao Huang Zhouying Lu +6 位作者 Fangbing Li Yang Deng Fangfang Wan Quancheng Wang Fousseni Folega Jinsong Wang Zijian Guo 《Forest Ecosystems》 SCIE CSCD 2024年第4期516-525,共10页
Understanding the drivers of variations in fine root lifespan is key to informing nutrient cycling and productivity in terrestrial ecosystems.However,the general patterns and determinants of forest fine root lifespan ... Understanding the drivers of variations in fine root lifespan is key to informing nutrient cycling and productivity in terrestrial ecosystems.However,the general patterns and determinants of forest fine root lifespan at the global scale are still limited.We compiled a dataset of 421 fine root lifespan observations from 76 tree species globally to assess phylogenetic signals among species,explored relationships between fine root lifespan and biotic and abiotic factors,and quantified the relative importance of phylogeny,root system structure and functions,climatic and edaphic factors in driving global fine root lifespan variations.Overall,fine root lifespan showed a clear phylogenetic signal,with gymnosperms having a longer fine root lifespan than angiosperms.Fine root lifespan was longer for evergreens than deciduous trees.Ectomycorrhizal(ECM)plants had an extended fine root lifespan than arbuscular mycorrhizal(AM)plants.Among different climatic zones,fine root lifespan was the longest in the boreal zone,while it did not vary between the temperate and tropical zone.Fine root lifespan increased with soil depth and root order.Furthermore,the analysis of relative importance indicated that phylogeny was the strongest driver influencing the variation in forest fine root lifespan,followed by soil clay content,root order,mean annual temperature,and soil depth,while other environmental factors and root traits exerted weaker effects.Our results suggest that the global pattern of fine root lifespan in forests is shaped by the interplay of phylogeny,root traits and environmental factors.These findings necessitate accurate representations of tree evolutionary history in earth system models to predict fine root longevity and its responses to global changes. 展开更多
关键词 Fine root lifespan Trees PHYLOGENY root order Soil property Climatic factors
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The glutamate receptor gene GLR3.3:A bridge of calciummediated root development in poplar
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作者 Yi An Ya Geng +5 位作者 Yu Liu Xiao Han Lichao Huang Wei Zeng Jin Zhang Mengzhu Lu 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第6期1449-1462,共14页
Poplar is one of the fastest-growing temperate trees in the world and is widely used in ornamental horticulture for shade.The root is essential for tree growth and development and its utilization potential is huge.Cal... Poplar is one of the fastest-growing temperate trees in the world and is widely used in ornamental horticulture for shade.The root is essential for tree growth and development and its utilization potential is huge.Calcium(Ca),as a signaling molecule,is involved in the regulation of plant root development.However,the detailed underlying regulatory mechanism is elusive.In this study,we analyzed the morphological and transcriptomic variations of 84K poplar(Populus alba×P.glandulosa)in response to different calcium concentrations and found that low Ca^(2+)(1 mmol·L^(-1))promoted lateral root development,while deficiency(0.1 mmol·L^(-1)Ca^(2+))inhibited lateral root development.Co-expression analysis showed that Ca^(2+)channel glutamate receptors(GLRs)were present in various modules with significance for root development.Two GLR paralogous genes,PagGLR3.3a and Pag GLR3.3b,were mainly expressed in roots and up-regulated under Ca^(2+)deficiency.The CRISPR/Cas9-mediated signal gene(crispr-PagGLR3.3a,PagGLR3.3b)and double gene(crispr-PagGLR3.3ab)mutants presented more and longer lateral roots.Anatomical analysis showed that crispr-PagGLR3.3ab plants had more xylem cells and promoted the development of secondary vascular tissues.Further transcriptomic analysis suggested that knockout of PagGLR3.3a and PagGLR3.3b led to the up-regulation of several genes related to protein phosphorylation,auxin efflux,lignin and hemicellulose biosynthesis as well as transcriptional regulation,which might contribute to lateral root growth.This study not only provides novel insight into how the Ca^(2+)channels mediated root growth and development in trees,but also provides a directive breeding of new poplar species for biofuel and bioenergy production. 展开更多
关键词 Glutamate receptor CALCIUM root development Lateral root POPLAR
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Shear resistance characteristics and influencing factors of root-soil composite on an alpine metal mine dump slope with different recovery periods
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作者 PANG Jinghao LIANG Shen +5 位作者 LIU Yabin LI Shengwei WANG Shu ZHU Haili LI Guorong HU Xiasong 《Journal of Mountain Science》 SCIE CSCD 2024年第3期835-849,共15页
Artificial vegetation restoration is the main measure for vegetation restoration and soil and water conservation in alpine mine dumps on the Qinghai-Tibet Plateau,China.However,there are few reports on the dynamic cha... Artificial vegetation restoration is the main measure for vegetation restoration and soil and water conservation in alpine mine dumps on the Qinghai-Tibet Plateau,China.However,there are few reports on the dynamic changes and the influencing factors of the soil reinforcement effect of plant species after artificial vegetation restoration under different recovery periods.We selected dump areas of the Delni Copper Mine in Qinghai Province,China to study the relationship between the shear strength and the peak displacement of the root-soil composite on the slope during the recovery period,and the influence of the root traits and soil physical properties on the shear resistance characteristics of the root-soil composite via in situ direct shear tests.The results indicate that the shear strength and peak displacement of the rooted soil initially decreased and then increased with the increase of the recovery period.The shear strength of the rooted soil and the recovery period exhibited a quadratic function relationship.There is no significant function relationship between the peak displacement and the recovery period.Significant positive correlations(P<0.05)exists between the shear strength of the root-soil composite and the root biomass density,root volume density,and root area ratio,and they show significant linear correlations(P<0.05).There are no significant correlations(P>0.05)between the shear strength of the root-soil composite and the root length density,and the root volume ratio of the coarse roots to the fine roots.A significant negative linear correlation(P<0.05)exists between the peak displacement of the rooted soil and the coarse-grain content,but no significant correlations(P>0.05)with the root traits,other soil physical property indices(the moisture content and dry density of the soil),and slope gradient.The coarse-grain content is the main factor controlling the peak displacement of the rooted soil. 展开更多
关键词 Alpine mine dump Artificial vegetation restoration period rooted soil Shear resistance characteristics root traits Soil physical properties
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Increasing root-lower characteristics improves drought tolerance in cotton cultivars at the seedling stage
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作者 Congcong Guo Hongchun Sun +7 位作者 Xiaoyuan Bao Lingxiao Zhu Yongjiang Zhang Ke Zhang Anchang Li Zhiying Bai Liantao Liu Cundong Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第7期2242-2254,共13页
Drought is an important abiotic stress factor in cotton production.The root system architecture(RSA)of cotton shows high plasticity which can alleviate drought-related stress under drought stress(DS)conditions;however... Drought is an important abiotic stress factor in cotton production.The root system architecture(RSA)of cotton shows high plasticity which can alleviate drought-related stress under drought stress(DS)conditions;however,this alleviation is cultivar dependent.Therefore,this study estimated the genetic variability of RSA in cotton under DS.Using the paper-based growth system,we assessed the RSA variability in 80 cotton cultivars at the seedling stage,with 0 and10%polyethylene glycol 6000(PEG6000)as the control(CK)and DS treatment,respectively.An analysis of 23 aboveground and root traits in the 80 cotton cultivars revealed different responses to DS.On the 10th day after DS treatment,the degree of variation in the RSA traits under DS(5–55%)was greater than that of CK(5–49%).The 80 cultivars were divided into drought-tolerant cultivars(group 1),intermediate drought-tolerant cultivars(group 2),and drought-sensitive cultivars(group 3)based on their comprehensive evaluation values of drought resistance.Under DS,the root lengthlower,root area-lower,root volume-lower,and root length density-lower were significantly reduced by 63,71,76,and 4%in the drought-sensitive cultivars compared to CK.Notably,the drought-tolerant cultivars maintained their root lengthlower,root area-lower,root volume-lower,and root length density–lower attributes.Compared to CK,the root diameter(0–2 mm)-lower increased by 21%in group 1 but decreased by 3 and 64%in groups 2 and 3,respectively,under DS.Additionally,the drought-tolerant cultivars displayed a plastic response under DS that was characterized by an increase in the root-lower characteristics.Drought resistance was positively correlated with the root area-lower and root length density-lower.Overall,the RSA of the different cotton cultivars varied greatly under DS.Therefore,important root traits,such as the root-lower traits,provide great insights for exploring whether drought-tolerant cotton cultivars can effectively withstand adverse environments. 展开更多
关键词 COTTON root system architecture drought stress cultivars variability root-lower
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Mechanism underlying the uprooting of taproot-type shrub species in the loess area of northeastern Qinghai-Xizang Plateau, China
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作者 LIANG Shen WANG Shu +4 位作者 LIU Yabin PANG Jinghao ZHU Haili LI Guorong HU Xiasong 《Journal of Arid Land》 SCIE CSCD 2024年第10期1426-1443,共18页
Characteristics of root pullout resistance determine the capacity to withstand uprooting and the slope protection ability of plants.However,mechanism underlying the uprooting of taproot-type shrub species in the loess... Characteristics of root pullout resistance determine the capacity to withstand uprooting and the slope protection ability of plants.However,mechanism underlying the uprooting of taproot-type shrub species in the loess area of northeastern Qinghai-Xizang Plateau,China remains unclear.In this study,a common taproot-type shrub,Caragana korshinskii Kom.,in northeastern Qinghai-Xizang Plateau was selected as the research material.Mechanism of root-soil interaction of vertical root of C.korshinskii was investigated via a combination of a single-root pullout test and numerical simulation analysis.The results indicated that,when pulling vertically,axial force of the roots decreased with an increase in buried depth,whereas shear stress at root-soil interface initially increased and then decreased as burial depths increased.At the same buried depth,both axial force and shear stress of the roots increased with the increase in pullout force.Shear stress and plastic zone of the soil surrounding the root were symmetrically distributed along the root system.Plastic zone was located close to the surface and was caused primarily by tensile failure.In nonvertical pulling,symmetry of shear stress and plastic zone of the soil surrounding the root was disrupted.We observed larger shear stress and plastic zones on the side facing the direction of root deflection.Plastic zone included both shear and tensile failure.Axial force of the root system near the surface decreased as deflection angle of the pullout force increased.When different rainfall infiltration depths had the same vertical pulling force,root axial force decreased with the increase of rainfall infiltration depth and total root displacement increased.During rainfall infiltration,shear stress and plastic zone of the soil surrounding the root were prone to propagating deeper into the soil.These findings provide a foundation for further investigation of soil reinforcement and slope protection mechanisms of taproot-type shrub species in the loess area of northeastern Qinghai-Xizang Plateau and similar areas. 展开更多
关键词 Qinghai-Xizang Plateau shrub roots pullout test numerical simulation MECHANISM root-soil interaction
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Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae
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作者 Wenlin Yu Lu Yang +12 位作者 Yuanyuan Xiang Rongde Li Xueqing Zhou Longcai Gan Xianyu Xiang Yunyun Zhang Lei Yuan Yanqing Luo Genze Li Youning Wang Yinhua Chen Peng Chen Chunyu Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期1049-1060,共12页
Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection.Breeding of clubroot resistant cultivars bas... Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection.Breeding of clubroot resistant cultivars based on mapping and cloning of resistant genes is commonly regarded as the most cost-effective and efficient way to fight against this disease.The traditional way of R gene functional validation requires stable transformation that is both time-and labor-consuming.In this study,a rapid and efficient hairy-root transgenic protocol mediated by Agrobacterium rhizogenes was developed.The transformation positive rate was over 80%in Brassica napus showed by GUS reporter gene and this transformation only took 1/6 of the time compared with stable transformation.The system was applicable to different B.napus varieties and other cruciferous crops including Brassica rapa and Brassica oleracea.In particular,two known CR genes,CRA3.7.1 and CRA8.2.4 were used respectively,as example to show that the system works well for CR gene study combined with subsequent P.brassicae infection in B.napus.Most importantly,it works both in over-expression that led to disease resistance,as well as in RNAi which led to disease susceptible phenotype.Therefore,this system can be used in batch-wise identification of CR genes,and also offered the possibility of manipulating key genes within the P.brassicae genome that could improve our knowledge on host-pathogen interaction. 展开更多
关键词 Brassicaceae Agrobacterium rhizogenes Hairy root transformation CLUBroot Gene function
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Pulp health and calcific healing of a complicated crown–root fracture with additional root fracture in a maxillary incisor: A case report
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作者 Na Li Yue-Yue Ren +4 位作者 Ying Tang Qi Yang Tian-Tian Meng Song Li Jing Zhang 《World Journal of Clinical Cases》 SCIE 2025年第3期42-49,共8页
BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and te... BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in theliterature.CASE SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical level.After the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival overgrowth.Cone-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival overgrowth.CONCLUSION This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture. 展开更多
关键词 Complicated crown-root fracture Multiple root fracture Spontaneous healing Cone-beam computed tomography Long-term follow-up Case report
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Hormonal and epigenetic regulation of root responses to salinity stress 被引量:1
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作者 Ping Yun Cengiz Kaya Sergey Shabala 《The Crop Journal》 SCIE CSCD 2024年第5期1309-1320,共12页
Salinity stress is a major environmental stress affecting crop productivity,and its negative impact on global food security is only going to increase,due to current climate trends.Salinity tolerance was present in wil... Salinity stress is a major environmental stress affecting crop productivity,and its negative impact on global food security is only going to increase,due to current climate trends.Salinity tolerance was present in wild crop relatives but significantly weakened during domestication.Regaining it back requires a good understanding of molecular mechanisms and traits involved in control of plant ionic and ROS homeostasis.This review summarizes our current knowledge on the role of major plant hormones(auxin,cytokinins,abscisic acid,salicylic acid,and jasmonate)in plants adaptation to soil salinity.We firstly discuss the role of hormones in controlling root tropisms,root growth and architecture(primary root elongation,meristematic activity,lateral root development,and root hairs formation).Hormone-mediated control of uptake and sequestration of key inorganic ions(sodium,potassium,and calcium)is then discussed followed by regulation of cell redox balance and ROS signaling in salt-stressed roots.Finally,the role of epigenetic alterations such as DNA methylation and histone modifications in control of plant ion and ROS homeostasis and signaling is discussed.This data may help develop novel strategies for breeding and cultivating salt-tolerant crops and improving agricultural productivity in saline regions. 展开更多
关键词 PHYTOHORMONE root development SODIUM POTASSIUM ROS signal
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The dorsal root ganglion as a target for neurorestoration in neuropathic pain 被引量:1
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作者 Guillermo Estivill-Torrús Ana Belen Martínez-Padilla +2 位作者 Lourdes Sánchez-Salido Anne Baron-Van Evercooren Beatriz García-Díaz 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期296-301,共6页
Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.... Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.During the processing of pain,the dorsal root ganglia constitute an important region where dorsal root ganglion neurons play a crucial role in the transmission and propagation of sensory electrical stimulation.Furthermore,the dorsal root ganglia have recently exhibited a regenerative capacity that should not be neglected in the understanding of the development and resolution of neuropathic pain and in the elucidation of innovative therapies.Here,we will review the complex interplay between cells(satellite glial cells and inflammatory cells)and factors(cytokines,neurotrophic factors and genetic factors)that takes place within the dorsal root ganglia and accounts for the generation of the aberrant excitation of primary sensory neurons occurring in neuropathic pain.More importantly,we will summarize an updated view of the current pharmacologic and nonpharmacologic therapies targeting the dorsal root ganglia for the treatment of neuropathic pain. 展开更多
关键词 CYTOKINES dorsal root ganglia genetic factors neuropathic pain neurotrophic factors pharmacologic and nonpharmacologic therapies satellite glial cells sensory neurons
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Involvement of the ABA-and H_(2)O_(2)-Mediated Ascorbate-Glutathione Cycle in the Drought Stress Responses of Wheat Roots 被引量:1
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作者 Mengyuan Li Zhongye Gao +2 位作者 Lina Jiang Leishan Chen Jianhui Ma 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第2期329-342,共14页
Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and th... Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and the AsA–GSH cycle under drought stress in wheat has not been studied.In this study,a hydroponic experiment was conducted in wheat seedlings subjected to 15%polyethylene glycol(PEG)6000–induced dehydration.Drought stress caused the rapid accumulation of endogenous ABA and H_(2)O_(2) and significantly decreased the number of root tips compared with the control.The application of ABA significantly increased the number of root tips,whereas the application of H_(2)O_(2) markedly reduced the number of root tips,compared with that under 15%PEG-6000.In addition,drought stress markedly increased the DHA,GSH and GSSG levels,but decreased the AsA levels,AsA/DHA and GSH/GSSG ratios compared with those in the control.The activities of the four enzymes in the AsA–GSH cycle were also markedly increased under drought stress,including glutathione reductase(GR),ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate reductase(DHAR),compared with those in the control.However,the application of an ABA inhibitor significantly inhibited GR,DHAR and APX activities,whereas the application of an H_(2)O_(2) inhibitor significantly inhibited DHAR and MDHAR activities.Furthermore,the application of ABA inhibitor significantly promoted the increases of H_(2)O_(2) and the application of H_(2)O_(2) inhibitor significantly blocked the increases of ABA,compared with those under 15% PEG-6000.Taken together,the results indicated that ABA and H_(2)O_(2) probably interact under drought stress in wheat;and both of them can mediate drought stress by modulating the enzymes in AsA–GSH cycle,where ABA acts as the main regulator of GR,DHAR,and APX activities,and H_(2)O_(2) acts as the main regulator of DHAR and MDHAR activities. 展开更多
关键词 ABA H_(2)O_(2) AsA-GSH cycle drought stress wheat roots
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Eff ects of stand condition and root density on fi ne-root dynamics across root functional groups in a subtropical montane forest 被引量:1
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作者 Lin Huang Rudong Zhao +3 位作者 Xiaoxiang Zhao Qiuxiang Tian Pengyun Yue Feng Liu 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第3期665-675,共11页
Fine roots play key roles in belowground C cycling in terrestrial ecosystems.Based on their distinct functions,fi ne roots are either absorptive fi ne roots(AFRs)or transport fi ne roots(TFRs).However,the function-bas... Fine roots play key roles in belowground C cycling in terrestrial ecosystems.Based on their distinct functions,fi ne roots are either absorptive fi ne roots(AFRs)or transport fi ne roots(TFRs).However,the function-based fi ne root dynamics of trees and their responses to forest stand properties remain unclear.Here,we studied the dynamics of AFRs and TFRs and their responses to stand conditions and root density in a subtropical montane mixed forest based on a 2-a root window experiment.Mean(±SE)annual production,mortality,and turnover rate of AFRs were 7.87±0.17 m m^(−2)a^(−1),8.13±0.20 m m^(−2)a^(−1)and 2.96±0.24 a^(−1),respectively,compared with 7.09±0.17 m m^(−2)a^(−1),4.59±0.17 m m^(−2)a^(−1),and 2.01±0.22 a^(−1),respectively,for TFRs.The production and mortality of fi ne roots were signifi cantly higher in high root-density sites than in low-root density sites,whereas the turnover of fi ne roots was faster in the low root-density sites.Furthermore,root density had a larger positive eff ect than other environmental factors on TFR production but had no obvious impact on AFR production.Tree species diversity had an apparent positive eff ect on AFR production and was the crucial driver of AFR production,probably due to a complementary eff ect,but had no evident impact on TFR.Both tree density and tree species diversity were positively correlated with the mortality of AFRs and negatively related to the turnover of TFRs,suggesting that higher root density caused stronger competition for rooting space and that plants tend to reduce maintenance costs by decreasing TFR turnover.These fi ndings illustrated the importance of root functional groups in understanding root dynamics and their responses to changes in environmental conditions. 展开更多
关键词 root functional group root production root mortality root turnover Stand condition Badagongshan mountain
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基于Root-MUSIC算法的激光扫频干涉测量方法研究
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作者 金陈凯 许新科 +3 位作者 刘易 何泽聪 宋云峰 何闻 《激光杂志》 CAS 北大核心 2024年第2期58-62,共5页
玻璃的厚度作为工业生产中的一项重要的指标,如何对其快速的测量成为了研究热点。提出采用激光扫频干涉测量方法,将采样的信号进行插值拟合,校正其拍频非线性,再通过频谱估计法获取距离信息。当测量厚度较小的玻璃时,FFT算法因受栅栏效... 玻璃的厚度作为工业生产中的一项重要的指标,如何对其快速的测量成为了研究热点。提出采用激光扫频干涉测量方法,将采样的信号进行插值拟合,校正其拍频非线性,再通过频谱估计法获取距离信息。当测量厚度较小的玻璃时,FFT算法因受栅栏效应和频谱干涉的影响,导致难以在频谱图中将玻璃前后表面的频率区分出来。为解决该问题,采用Root-MUSIC算法处理信号,实验结果表明在FFT算法分辨临近目标频率失效的情况下,Root-MUSIC算法可以进行有效区分,运算效率也远高于MUSIC算法,且精度可达50微米左右,为解决玻璃厚度测量问题提供了思路。 展开更多
关键词 激光扫频干涉测距 厚度测量 root-MUSIC算法 测距
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The osteoclastic activity in apical distal region of molar mesial roots affects orthodontic tooth movement and root resorption in rats
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作者 Wenhao Zheng Xiaofeng Lu +9 位作者 Guangjin Chen Yufeng Shen Xiaofei Huang Jinfeng Peng Jiajia Wang Ying Yin Wencheng Song Mengru Xie Shaoling Yu Lili Chen 《International Journal of Oral Science》 SCIE CAS CSCD 2024年第2期322-332,共11页
The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic treatment.However,the sensitivity of existing detection techniques is ... The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic treatment.However,the sensitivity of existing detection techniques is not sufficient,and the criteria for evaluating optimal force have not been yet established.Here,by employing 3D finite element analysis methodology,we found that the apical distal region(A-D region)of mesial roots is particularly sensitive to orthodontic force in rats.Tartrate-resistant acidic phosphatase(TRAP)-positive osteoclasts began accumulating in the A-D region under the force of 40 grams(g),leading to alveolar bone resorption and tooth movement.When the force reached 80 g,TRAP-positive osteoclasts started appearing on the root surface in the A-D region.Additionally,micro-computed tomography revealed a significant root resorption at 80 g.Notably,the A-D region was identified as a major contributor to whole root resorption.It was determined that 40 g is the minimum effective force for tooth movement with minimal side effects according to the analysis of tooth movement,inclination,and hyalinization.These findings suggest that the A-D region with its changes on the root surface is an important consideration and sensitive indicator when evaluating orthodontic forces for a rat model.Collectively,our investigations into this region would aid in offering valuable implications for preventing and minimizing root resorption during patients’orthodontic treatment. 展开更多
关键词 MOVEMENT rootS offering
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Overexpression of the peroxidase gene ZmPRX1 increases maize seedling drought tolerance by promoting root development and lignification
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作者 Xiuzhen Zhai Xiaocui Yan +6 位作者 Tinashe Zenda Nan Wang Anyi Dong Qian Yang Yuan Zhong Yue Xing Huijun Duan 《The Crop Journal》 SCIE CSCD 2024年第3期753-765,共13页
Drought is a main abiotic stress factor hindering plant growth,development,and crop productivity.Therefore,it is crucial to understand the mechanisms by which plants cope with drought stress.Here,the function of the m... Drought is a main abiotic stress factor hindering plant growth,development,and crop productivity.Therefore,it is crucial to understand the mechanisms by which plants cope with drought stress.Here,the function of the maize peroxidase gene ZmPRX1 in drought stress tolerance was investigated by measurement of its expression in response to drought treatment both in a ZmPRX1 overexpression line and a mutant line.The higher root lignin accumulation and seedling survival rate of the overexpression line than that of the wild type or mutant support a role for ZmPRX1 in maize drought tolerance by regulating root development and lignification.Additionally,yeast one-hybrid,Dule luciferase and ChIP-qPCR assays showed that ZmPRX1 is negatively regulated by a nuclear-localized ZmWRKY86 transcription factor.The gene could potentially be used for breeding of drought-tolerant cultivars. 展开更多
关键词 Drought tolerance MAIZE ZmPRX1 root development Lignin biosynthesis
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