Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season...Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season rice(DDR) in Central China. The objective of this study was to evaluate the effects of various N and seeding rates on the grain yield and NUE of an ultrashort-duration variety grown under DDR. Field experiments were conducted in 2018 in Wuxue County and 2019 in Qichun County, Hubei Province, China with four N rates and three seeding rates.The results showed that the grain yield of the ultrashort-duration variety ranged from 6.32 to 8.23 t ha–1with a total growth duration of 85 to 97 days across all treatments with N application. Grain yield was increased significantly by N application in most cases, but seeding rate had an inconsistent effect on grain yield. Furthermore, the response of grain yield to the N rates was much higher than the response to seeding rates. The moderate N rates of 100–150 and 70–120 kg N ha–1in the early and late seasons, respectively, could fully express the yield potential of the ultrashort-duration variety grown under DDR. Remarkably higher N responses and agronomic NUE levels were achieved in the early-season rice compared with the late-season rice due to the difference in indigenous soil N supply capacity(INS) between the two seasons. Seasonal differences in INS and N response should be considered when crop management practices are optimized for achieving high grain yield and NUE in ultrashort-duration variety grown under DDR.展开更多
The paper was to study the effects of seeding rate, water and fertilizer ( N, P, K) coupling on grass yield of forage millet Jigu No. 18 (Setaria itlica). A quadratic regression otthogonal rotation combination wit...The paper was to study the effects of seeding rate, water and fertilizer ( N, P, K) coupling on grass yield of forage millet Jigu No. 18 (Setaria itlica). A quadratic regression otthogonal rotation combination with five factors was designed in pot experiment. The mathematical model between hay yield of forage millet (Y) and soil moisture content ( x1 ), N fertilizer (x2 ), P fertilizer (x3 ), K fertilizer (x4) and seeding rate (x5 ) was established to simulate optimization. The results showed that moisture content, seeding rate, P fertilizer and K fertilizer had important effects on hay yield. Soil moisture content had the biggest impact on yield, followed by seeding rate, P and K fertilizer. The coupling effects of various factors successively were moisture content / seeding rate 〉 K fertil- izer/seeding rate 〉 N / P fertilizer 〉 soil moisture/N fertilizer 〉 soil moisture/ P fertilizer. Moreover, the mathematical model, Y = 20 543. 756 - 565. 570xI -39. 942x2 -23. 102x3 -38. 470x4 - 151. 877x5 + 1. 052x^x2 + 1. 604xIx3 + 12. 953xt x5 - 0. 173x2x3 + 0. 737x4x5 - 2. 292x5^2, was established. The optimum soil moisture and seeding rate were determined as 10% andl5 kg/hm2, respectively. In this scheme, the hay yield was 14 037. 151 0 kg/hm^2 and the economic benefit was 13 887.15 yuan/hm^2 ; the income was increased by 23.68% ( 3 288.98 yuan/hm^2 ) compared to the optimal combination in the test. The results provided a theoretical basis and technical support for forage millet production in Hebei Province.展开更多
[Objectives]This study was conducted to investigate the effects of seeding rate and light on the seed yield and active ingredients of Vernonia anthelmintica.[Methods]With V.anthelmintica L.Willd as the research object...[Objectives]This study was conducted to investigate the effects of seeding rate and light on the seed yield and active ingredients of Vernonia anthelmintica.[Methods]With V.anthelmintica L.Willd as the research object,the seed yield was determined by staged harvesting combined with the actual cutting and measuring method;and the caffeoylquinic acids(CQA)were used as the characterization indexes,and high performance liquid chromatography was used to determine the contents of 3,4-CQA,3,5-CQA and 4,5-CQA of V.anthelmintica seeds under different seeding rate+light treatments,so as to compare the changes in seed yield and chemical composition of V.anthelmintica under different seeding rates and light factors.[Results]In Moyu County,Hetian and similar areas,the suitable planting rate of V.anthelmintica was in the range of 13.5-15.0 kg/hm^(2).For different harvesting purposes,the optimal seeding rate and light conditions of V.Anthelmintica were different.[Conclusions]The seeding rate and light conditions can be used to interfere with the seed yield and quality of V.anthelmintica.展开更多
<span style="font-family:Verdana;">A field trial was conducted at a private farm in AL-Hashimiya district Babylon Governorate—the republic of Iraq during the 2016</span><span style="font...<span style="font-family:Verdana;">A field trial was conducted at a private farm in AL-Hashimiya district Babylon Governorate—the republic of Iraq during the 2016</span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;">2017 and 2017</span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">2018 growing seasons.</span><span style="color:red;"> </span><span style="font-family:Verdana;">This study was conducted using two irrigation methods, sprinkler and surface irrigation, for each of them had three Tillage methods (zero-tillage</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> medium-tillage</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> deep-tillage) and each tillage system had four seeding rate of wheat yield (120</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">180</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">240</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">300) kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">Results indicated that the consumptive water use was 557.5 and</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">535.9 mm for surface irrigation and 460.9 and 442.6 mm for sprinkler irrigation in </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">2016-2017 and 2017-2018 growing seasons. Sprinkler irrigation significantly increased the flag leaf area with no significant effect on plant height. However, the minimum tillage and seeding rate (240 kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;">) significantly increased the plant height and flag leaf </span><span style="font-family:Verdana;">area in both growing seasons. For the grain yield, the sprinkler irrigation, m</span><span style="font-family:Verdana;">inimum tillage, and seeding rate (240 </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;">) also increased the plant height and flag leaf area by 13%, 10, % 11%, 11%, 12%, and 14% in both growing seasons, respectively, through an increased number of spikes/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, the number of grain spike-1, and 1000-grain weight in both growing seasons, respe</span></span><span style="font-family:Verdana;">ctively. Interestingly the grain yield was increased by 33% and 32% in both growing seasons under the effects of these three factors altogether, respectively. It can be concluded that these factors act synergistically, resulting in a significant improvement in the wheat grain-yield of, less consumptive water use, and high water use efficiency.</span>展开更多
In this study,Zhongzao 39,Zhuliangyou 189,Hyou 518 and Taiyou 390 four rice cultivars were used to explore the effects of increased seedling age on the seedling quality,tillering rate,panicle-bearing tiller rate,bioma...In this study,Zhongzao 39,Zhuliangyou 189,Hyou 518 and Taiyou 390 four rice cultivars were used to explore the effects of increased seedling age on the seedling quality,tillering rate,panicle-bearing tiller rate,biomass,and seed yield attributes with a low seeding rate grown under machine-transplanted conditions.The results showed that compared to 20-day-old(15)rice seedlings with a conventional seeding rate,rice seedlings with a low seeding rate showed a significantly increased grain yield at higher seedling ages(5~10 d).In seedlings with a low seeding rate,the average number of spikelets per panicle was 18.9%higher in 25-day-old early rice and 15.9%higher in 20-day-old late rice compared to seedlings with a conventional seeding rate.There was no significant difference in panicles per square meter between the seedlings with a low seeding rate and the seedlings with a conventional seeding rate.In the low seeding rate plants,an increased seedling age(5~10 d)resulted in higher seedling quality and higher tillering and panicle-bearing tiller rates.Our results suggested that the prolonged aging of the seedlings with a low seeding rate compensated for the negative influence of delayed transplantation under machine-transplanted conditions.展开更多
To adapt to the diversity of hybrid rice and meet its requirements of precision seeding,a rice pneumatic seeder with adjustable seeding rates was designed in this study.The seeder employed an independent vortex pump t...To adapt to the diversity of hybrid rice and meet its requirements of precision seeding,a rice pneumatic seeder with adjustable seeding rates was designed in this study.The seeder employed an independent vortex pump to provide the air source for the seed metering device.According to the requirements of air flow in the seed metering device,a sub-pipeline and a general pipeline were designed.The performances of the two types of pipelines were verified by the air pressure uniformity tests.The results showed that the sub-pipeline can distribute the air pressure more evenly than the general pipeline.The coefficient of variation of the sub-pipeline pressure was less than 5%.Wuyou 1179 hybrid rice was selected as the experimental object.The field seeding tests were carried out with different negative pressures and different numbers of groups of sucking holes as experimental factors.The results showed that the average qualified rate of seeding was 93.35%when only 2 groups of sucking holes were working under the seed sucking negative pressure of 2.0 kPa.The average qualified rate of seeding was 87.21%when 3 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The average qualified rate of seeding was 83.73%when 4 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The average qualified rate of seeding was 79.86%when 5 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The main factors affecting the seeding effect were analyzed and the feasibility of a rice pneumatic seeder with adjustable seeding rates was verified by field tests.The research results can provide a basis for its practical production and application.展开更多
In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the grow...In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the growth characteris- tics, quality and root twining power of machine-transplanted rice seedlings were studied. The results showed that seed rate had great effects on root twining power and qualityof rice seedlings; with the increase of seed rate, the root twining power of rice seedlings was increased, but the quality of rice seedlings was significantly reduced; under the condition of high seed rate, rice seedlings were weak with less accumulate dry matter and nonuniform growth, and the proportion of weak seedlings was higher. When the seed rate of both rice cultivars was reduced by 10 g/tray, the average seedling height was increased by 0.5 cm, leaf age was increased by 0.1, stem base diameter was increased by 0.1 cm, and root number per plant was increased by 0.3. When the seed rate was increased by 10 g/tray, the accumulated dry matter per plant was reduced by 5.1 mg. With the increase of seed rate, the seedling quality of Zhongzheyou No.1 was decreased rapidly, and the proportion of weak seedlings was increased rapidly. However, Yongyou 12 has a larger elasticity in seed rate. When the seed rate reached to 50 g/tray, blanket seedlings of Yongy- ou 12 were nursed with uniform growth and higher quality, but the shoots were sparse with lower leaf area index. However, when the seed rate was up to 50 g/tray, blanket seedlings of Zhongzheyou No.1 were nursed with uniform growth and higher quality, as well as higher-density shoots. From a comprehensive point of view, the optimum seed rates of Yongyou 12 and Zhongzheyou No.1 were 65 and 50 g/tray, respectively.展开更多
The seed setting rates of total 198 rice cultivars (lines) at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme na...The seed setting rates of total 198 rice cultivars (lines) at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme natural high temperature on seed setting rate of different rice cultivar (line). The results showed that the contin- uous high temperature showed certain effects on the seed setting rates of tested materials, and significant differences were shown in seed setting rate among differ- ent rice cultivars (lines). The seed setting rates differed significantly among indica F1 hybrids derived from different sterile and restorer lines, indicating that the sterile and restorer lines had great effects on heat tolerances of different F~ hybrids. The cor- relation analysis showed that the seed setting rates of conventional indica restorer lines and conventional japonica rice cultivars (lines) were negatively related to the daily highest temperature (P〉0.05), and the seed setting rates of indica F1 hybrids were positively related to the seed setting rates of their restorer lines. Total four in- dica restorer lines, including Ninghuiguangkangzhan, Shuhui 527, Chenghui 3203 and Xianyin-8, and four new japonica rice cultivars (lines), including Wuyinjinghui (B2), Nanjing 4//W3660/Nanjing 44 (B12) and Wuyun 2330/JD6011 (B22) were pre- liminarily screened, and their seed setting rates were all close to the normal level (90%). The screened rice cultivars (lines) showed higher heat tolerances.展开更多
[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new ...[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps (control) were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation.展开更多
Rice(Oryza sativa)plants acquired excess photosynthates in the form of nonstructural carbohydrates(NSCs)in their stems and grain.Despite keen interest in rice NSC,the dynamics of NSC accumulation,translocation and re-...Rice(Oryza sativa)plants acquired excess photosynthates in the form of nonstructural carbohydrates(NSCs)in their stems and grain.Despite keen interest in rice NSC,the dynamics of NSC accumulation,translocation and re-accumulation have not yet been well investigated.AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds.Here we conducted three experiments,greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice(DSR)system,using elite lines incorporating AG1,AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On(AG1)and Ma-Zhan Red(AG2).In germinating seedlings,soluble sugars increased,while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil.Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage.But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017.Plant survival rate was positively correlated with the stem NSC at the early vegetative stage(21 days after sowing),and stem NSC was positively associated with plant height at different growth stages.Among the tested seeding rate,the most suitable seeding rate,4 g/m2 with shallow burial depth(0.5 cm),resulted in better seedling establishment,relatively higher seedling vigor index and higher leaf area index under flooding in DSR system.Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate,germination rate,and growth and biomass production.展开更多
To explore the relation of maize grain texture and phenotypic traits with grain thin-layer drying rate,we observed the ultra-structure of maize grain,and tested three traits about the maize grain texture and four phen...To explore the relation of maize grain texture and phenotypic traits with grain thin-layer drying rate,we observed the ultra-structure of maize grain,and tested three traits about the maize grain texture and four phenotypic traits.The vitreous part percentage was different(P〈0.05) among different maize inbred lines.There was a significant relationship between the drying rate with grain texture and phenotypic traits.Main factors that influenced the drying rate were different during different drying stages.New results observed that empirical constants(k and N) in drying equation were different for seed of the 30 inbred lines of maize.The k of simplified diffusion equation and N of page equation were significantly influenced by both grain texture and phenotypic traits.These results could be used as guideline parameters for drying maize seeds having different grain characteristics during different drying stages.展开更多
This study aimed to investigate the effects of treatments of rice pollen with different solvents on the seed setting rate.Rice pollen was treated with ultrapure water,0.5%dimethyl sulfoxide,ethanol(1%,5%,and 8%),and 0...This study aimed to investigate the effects of treatments of rice pollen with different solvents on the seed setting rate.Rice pollen was treated with ultrapure water,0.5%dimethyl sulfoxide,ethanol(1%,5%,and 8%),and 0.5%dimethyl sulfoxide+8%ethanol,and the pollen without treatment was taken as the control.Then,crossing was conducted with the treated rice plants as the male parents and plants subjected to emasculation by warm water as the female parents.The seed setting rates of the male and female parents were then recorded.The results showed that the treatments with different solvents significantly decreased the seed setting rates of both male and female parents.The treatment with 1%ethanol resulted in the highest seed setting rates for both male and female parents,while the treatment with 0.5%dimethyl sulfoxide resulted in the lowest seed setting rates for both parents.Although these solvents affected the seed setting rate of rice,they served as intermediate solvents to dissolve chemicals used in the induction of rice haploids.This study layed a technical foundation for subsequent chemical induction of rice haploids.展开更多
The influences of N application rate, timing, sowing date and seeding rate on β-glucan and protein content in barley grains were studied through the field experiments in Hangzhou, China during 1997 -2001. Protein con...The influences of N application rate, timing, sowing date and seeding rate on β-glucan and protein content in barley grains were studied through the field experiments in Hangzhou, China during 1997 -2001. Protein content increased with N application rate and with N proportion applied at late stage. β-glucan content also responded significantly to N application rate and timing, but with different pattern with protein content. Of three N rate treatments, the medium N rate (135 kg ha-1) had the highest β-glucan content, being significantly higher than low N rate (90 kg ha-1) and no difference with high N rate (180 kg ha-1). A-mong three N timing treatments, two times of N top-dressing at both tillering and booting stage had significantly higher β-glucan content than once N top-dressing at tillering or booting stage. Sowing date has the dramatic effect on both β-glucan and protein content. Protein content decreased with the delayed sowing, and kernel weight showed opposite tendency. Either earlier or later sowing caused increased β-glucan content relative to sowing in early November, which is the normal date for barley sowing locally. Seeding rate had no significant influence on both β-glucan and protein content.展开更多
[Objective] The paper was to explore the flowering habits and fertility of Gaozhou common wild rice (Oryza rufipogon Griff.) in Lingshui of Hainan Province. [Method] The flowering habits, pollen fertility and seed s...[Objective] The paper was to explore the flowering habits and fertility of Gaozhou common wild rice (Oryza rufipogon Griff.) in Lingshui of Hainan Province. [Method] The flowering habits, pollen fertility and seed setting rate of Gaozhou common wild rice in Lingshui of Hainan Province were studied, and compared with the flowering habits of common wild rice in Hainan Province. [Result] Gaozhou common wild rice was able to flower twice a year. The first flowering stage was from mid-April to mid-June for about 60 d, and the daily peak flowering dates were from May 13 to May 22. The daily flowering time was mainly between 11:20 and 13:00, and the peak flowering was at 11:30. The second flowering stage was from late August to late October for about 45 d, and the peak flowering dates lasted from September 8 to September 18. The daily flowering time was mainly from 11:15 to 12:50, and the daily peak flowering was at 11:30. In both flowering stages, the panicles had the same flowering characteristics. The stigma exsertion reached a high value of 95.37% with single (46.23%) and dual (49.14%) stigmas exsertion respectively. A spikelet remained open for 1.5-2.5 h, the angle of spikelet opening varied from 29.9° to 32.4°. At the two flowering stage the average pollen fertility rates were 89.67% and 85.62%, the average seed setting rates were 58.81% and 55.79%, respectively. Hainan population flowered only once (from mid-October to mid-December), this had significant difference with the performance of Gaozhou common wild rice which flowered twice a year. [Conclusion] The results provided basis for the research on gene flow frequency from transgenic rice to wild rice and its relationship with flowering habits of common wild rice.展开更多
When being planted in wide areas in southern China (23°23' - 33°23' N, 98°35' - 120°19' E, sea level above 2.7 - 1285 m), Liangyoupeijiu (LYPJ), a two-line hybrid rice combination, ...When being planted in wide areas in southern China (23°23' - 33°23' N, 98°35' - 120°19' E, sea level above 2.7 - 1285 m), Liangyoupeijiu (LYPJ), a two-line hybrid rice combination, showed a seed setting rate of 75.2 - 77.2%, which was lower by 4.3 - 7.5 percent point than that of an indica hybrid rice Shanyou 63 (CK), with similar values of grain yield and coefficients of variation to CK. Sowing during 5-25th of May in Nanjing (32°3' N, 118°48' E), China, LYPJ headed before 4th September, and gave a seed setting rate of 75 - 90%, and grain yield over 1 kg/m2. If the sowing date was delayed to 14 - 15th, June, its heading date would be as late as 17th - 21st September, and seed setting rate would be declined by 10 - 15% in comparison with that on a suitable sowing date. When flowering took place at an average daily temperature range of 13.7 - 28.5℃ for five days, the spikelet fertility (SF) would be increased by 1.9- 10.7%, for each increment of 1℃. The suitable (SF≥90%) and safe (SF≥75%) temperatures for flowering stage were indicated to be 26.5℃ and 22.9℃, respectively. To get a high and stable seed set in cultivating LYPJ, it was recommended that LYPJ would be planted in the areas south of 34癗, and the favorable average daily temperature during flowering stage should be at 26 - 28℃.展开更多
The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice (Oryza sativa L.), including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning c...The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice (Oryza sativa L.), including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy (WCLSM). Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and 'double set' of embryo sacs. The frequency of abnormal embryo sac in japonica (26.6%) was higher than that in indica (19.34%). In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.展开更多
The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on se...The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5-10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10-15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable.展开更多
Na4EDTA and EDTA were adopted as new additives to intensify the seeded precipitation process of sodium aluminate solution. The effects of the two additives at certain concentrations on the seeded precipitation rate of...Na4EDTA and EDTA were adopted as new additives to intensify the seeded precipitation process of sodium aluminate solution. The effects of the two additives at certain concentrations on the seeded precipitation rate of sodium aluminate solution, particle size distribution (PSD) and morphology of precipitated gibbsite were investigated using titration method, particle size analyzer and scanning electron microscope (SEM), respectively. The results show that the two additives can accelerate the seeded precipitation rate of sodium aluminate solution. At relatively high concentration, the facilitative effect of EDTA on sodium aluminate solution is more obvious than that of Na4EDTA. EDTA makes gibbsite particles thinner than Na4EDTA. The Na+ and H+ result in the different effects on the seeded precipitation rate of sodium aluminate solution in spite of the same EDTA anion in the two additives.展开更多
The field experiment was conducted at Pulses Research Centre of Bangladesh Agricultural Institute (BARI), Ishurdi, Pabna, Bangladesh during rabi season of 2009 to find out the suitable management package for better ...The field experiment was conducted at Pulses Research Centre of Bangladesh Agricultural Institute (BARI), Ishurdi, Pabna, Bangladesh during rabi season of 2009 to find out the suitable management package for better plant establishment and higher yield of lentil under relay cropping. The experiment was based on three genotypes--Rajbari local, X95S-167(4) and X95S-136; two types of rice straw height--I 0 cm and 30 cm, three seed rates (kg/ha)--e.g., 30, 50 and 60, and three levels of fertilizer--control, 30-60-20 kg/ha of N-P2Os-K20 and 30-60-30 kg/ha of N-P2Os-KzO. The experiment was laid out in a split-split-split-plot design with three replications. Genotype was placed in the main-plot, rice straw height was in the sub-plot, seed rate was in the sub-sub-plot and fertilizer level was in the sub-sub-sub-plot. From this study, it was observed that lentil genotype X95S-136X planted with a rice straw height under 30 cm, at a seed rate of 50 kg/ha, and with a fertilizer level at 30-60-30 kg/ha of N-P2Os-K20 produced the highest lentil seed yield of 2,070 kg/ha and the lowest seed yield (550 kg/ha ) was obtained from genotype Rajbari local, planted with a rice straw height under 10 cm, at a seed rate of 30 kg/ha and no fertilizer. Similar result regarding seed and straw yield was also found in the farmer's field. The accession X95S-136 along with improved production technologies produced the highest grain and straw yield, and net return but the lowest grain and straw yield as well as net return were obtained by Rajbari local in the demonstration field. It was also identified that, by the inclusion of lentil in the transplanted monsoon rice field, lands could be brought under lentil cultivation to enhance lentil production, provide human nutrition and also to ensure soil health improvement for sustainable production system.展开更多
Comparison of seed setting rate, plant height and Cheng's morphological traits were made between the F_2 progeny of indica-japonica hybrids, 02428/Pei'ai 64 and Taichung 65/Teqing. There existed resemblance no...Comparison of seed setting rate, plant height and Cheng's morphological traits were made between the F_2 progeny of indica-japonica hybrids, 02428/Pei'ai 64 and Taichung 65/Teqing. There existed resemblance normal distribution in plant height, and the distribution had no relationship with the seed setting rate and plant height in F_1 progeny, suggesting that there were no gametic selection for the plant height in the F_2 progeny of indica-japoniea hybrids. An average seed setting rate of the F_2 was equal to the F_1 populations in the recombination of 02428/Pei'ai 64 in which there was an excellent cross compatibility, but the remarkable increase in the seed setting rate from the F_1 to F_2 in Taichung 65/Teqing, indicating that gametic selection only took place in the indica-japonica crosses with low cross compatibility while null or little in the hybrids with high compatibility. There were many intermediate types among the leaf pubescence, color of hull at heading, and length of the first and second panicle internode, and these Cheng's morphological traits was distributed normally; there were also a little intermediate types in hull hairness and phenol reaction with bi-peak distribution; while length to width ratio of grain was inclined to the japonica types obviously showing a skewed distribution in the F2 population. Although Cheng's morphological traits in F_2 populations had inconsistent distribution types, Cheng's morphological traits index showed normal distribution, which suggested that intermediate types, i.e. indicalinous or japonicalinous, were primary in F_2 population.展开更多
基金supported by the National Natural Science Foundation of China(31971845 and 32101819)the earmarked fund for China Agriculture Research System(CARS-01-20)the China Postdoctoral Science Foundation(2021M691179)。
文摘Nitrogen(N) and seeding rates are important factors affecting grain yield and N use efficiency(NUE) in directseeded rice. However, these factors have not been adequately investigated on direct-seeded and double-season rice(DDR) in Central China. The objective of this study was to evaluate the effects of various N and seeding rates on the grain yield and NUE of an ultrashort-duration variety grown under DDR. Field experiments were conducted in 2018 in Wuxue County and 2019 in Qichun County, Hubei Province, China with four N rates and three seeding rates.The results showed that the grain yield of the ultrashort-duration variety ranged from 6.32 to 8.23 t ha–1with a total growth duration of 85 to 97 days across all treatments with N application. Grain yield was increased significantly by N application in most cases, but seeding rate had an inconsistent effect on grain yield. Furthermore, the response of grain yield to the N rates was much higher than the response to seeding rates. The moderate N rates of 100–150 and 70–120 kg N ha–1in the early and late seasons, respectively, could fully express the yield potential of the ultrashort-duration variety grown under DDR. Remarkably higher N responses and agronomic NUE levels were achieved in the early-season rice compared with the late-season rice due to the difference in indigenous soil N supply capacity(INS) between the two seasons. Seasonal differences in INS and N response should be considered when crop management practices are optimized for achieving high grain yield and NUE in ultrashort-duration variety grown under DDR.
基金Special Fund for Agro-scientific Research in the Public Interest Research and Demonstration of Development and Utilization Technology of Forage Feed Resources in Pastoral Areas(20120304201)
文摘The paper was to study the effects of seeding rate, water and fertilizer ( N, P, K) coupling on grass yield of forage millet Jigu No. 18 (Setaria itlica). A quadratic regression otthogonal rotation combination with five factors was designed in pot experiment. The mathematical model between hay yield of forage millet (Y) and soil moisture content ( x1 ), N fertilizer (x2 ), P fertilizer (x3 ), K fertilizer (x4) and seeding rate (x5 ) was established to simulate optimization. The results showed that moisture content, seeding rate, P fertilizer and K fertilizer had important effects on hay yield. Soil moisture content had the biggest impact on yield, followed by seeding rate, P and K fertilizer. The coupling effects of various factors successively were moisture content / seeding rate 〉 K fertil- izer/seeding rate 〉 N / P fertilizer 〉 soil moisture/N fertilizer 〉 soil moisture/ P fertilizer. Moreover, the mathematical model, Y = 20 543. 756 - 565. 570xI -39. 942x2 -23. 102x3 -38. 470x4 - 151. 877x5 + 1. 052x^x2 + 1. 604xIx3 + 12. 953xt x5 - 0. 173x2x3 + 0. 737x4x5 - 2. 292x5^2, was established. The optimum soil moisture and seeding rate were determined as 10% andl5 kg/hm2, respectively. In this scheme, the hay yield was 14 037. 151 0 kg/hm^2 and the economic benefit was 13 887.15 yuan/hm^2 ; the income was increased by 23.68% ( 3 288.98 yuan/hm^2 ) compared to the optimal combination in the test. The results provided a theoretical basis and technical support for forage millet production in Hebei Province.
基金Autonomous Region Science and Technology Supporting Xinjiang Project Plant Project(2016E02007)the West Light Foundation of The Chinese Academy of Sciences(2019-FPGGRC-004).
文摘[Objectives]This study was conducted to investigate the effects of seeding rate and light on the seed yield and active ingredients of Vernonia anthelmintica.[Methods]With V.anthelmintica L.Willd as the research object,the seed yield was determined by staged harvesting combined with the actual cutting and measuring method;and the caffeoylquinic acids(CQA)were used as the characterization indexes,and high performance liquid chromatography was used to determine the contents of 3,4-CQA,3,5-CQA and 4,5-CQA of V.anthelmintica seeds under different seeding rate+light treatments,so as to compare the changes in seed yield and chemical composition of V.anthelmintica under different seeding rates and light factors.[Results]In Moyu County,Hetian and similar areas,the suitable planting rate of V.anthelmintica was in the range of 13.5-15.0 kg/hm^(2).For different harvesting purposes,the optimal seeding rate and light conditions of V.Anthelmintica were different.[Conclusions]The seeding rate and light conditions can be used to interfere with the seed yield and quality of V.anthelmintica.
文摘<span style="font-family:Verdana;">A field trial was conducted at a private farm in AL-Hashimiya district Babylon Governorate—the republic of Iraq during the 2016</span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;">2017 and 2017</span><span style="font-family:Verdana;">-</span><span style="font-family:;" "=""><span style="font-family:Verdana;">2018 growing seasons.</span><span style="color:red;"> </span><span style="font-family:Verdana;">This study was conducted using two irrigation methods, sprinkler and surface irrigation, for each of them had three Tillage methods (zero-tillage</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> medium-tillage</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> deep-tillage) and each tillage system had four seeding rate of wheat yield (120</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">180</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">240</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">300) kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">.</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">Results indicated that the consumptive water use was 557.5 and</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">535.9 mm for surface irrigation and 460.9 and 442.6 mm for sprinkler irrigation in </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">2016-2017 and 2017-2018 growing seasons. Sprinkler irrigation significantly increased the flag leaf area with no significant effect on plant height. However, the minimum tillage and seeding rate (240 kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;">) significantly increased the plant height and flag leaf </span><span style="font-family:Verdana;">area in both growing seasons. For the grain yield, the sprinkler irrigation, m</span><span style="font-family:Verdana;">inimum tillage, and seeding rate (240 </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">kg<span style="white-space:nowrap;">∙</span>ha</span><sup><span style="font-family:Verdana;">-1</span></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;">) also increased the plant height and flag leaf area by 13%, 10, % 11%, 11%, 12%, and 14% in both growing seasons, respectively, through an increased number of spikes/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, the number of grain spike-1, and 1000-grain weight in both growing seasons, respe</span></span><span style="font-family:Verdana;">ctively. Interestingly the grain yield was increased by 33% and 32% in both growing seasons under the effects of these three factors altogether, respectively. It can be concluded that these factors act synergistically, resulting in a significant improvement in the wheat grain-yield of, less consumptive water use, and high water use efficiency.</span>
文摘In this study,Zhongzao 39,Zhuliangyou 189,Hyou 518 and Taiyou 390 four rice cultivars were used to explore the effects of increased seedling age on the seedling quality,tillering rate,panicle-bearing tiller rate,biomass,and seed yield attributes with a low seeding rate grown under machine-transplanted conditions.The results showed that compared to 20-day-old(15)rice seedlings with a conventional seeding rate,rice seedlings with a low seeding rate showed a significantly increased grain yield at higher seedling ages(5~10 d).In seedlings with a low seeding rate,the average number of spikelets per panicle was 18.9%higher in 25-day-old early rice and 15.9%higher in 20-day-old late rice compared to seedlings with a conventional seeding rate.There was no significant difference in panicles per square meter between the seedlings with a low seeding rate and the seedlings with a conventional seeding rate.In the low seeding rate plants,an increased seedling age(5~10 d)resulted in higher seedling quality and higher tillering and panicle-bearing tiller rates.Our results suggested that the prolonged aging of the seedlings with a low seeding rate compensated for the negative influence of delayed transplantation under machine-transplanted conditions.
基金The authors are thankful for funding from the National Natural Science Foundation of China under Grant No.31871529,the earmarked fund for the National key research program of China under Grant No.2017YFD07000700,2017YFD07000704,and China Agriculture Research System under Grant CARS-01-41.The authos also acknowledge the anonymous reviewers for their critical comments and suggestions for improving the manuscript.
文摘To adapt to the diversity of hybrid rice and meet its requirements of precision seeding,a rice pneumatic seeder with adjustable seeding rates was designed in this study.The seeder employed an independent vortex pump to provide the air source for the seed metering device.According to the requirements of air flow in the seed metering device,a sub-pipeline and a general pipeline were designed.The performances of the two types of pipelines were verified by the air pressure uniformity tests.The results showed that the sub-pipeline can distribute the air pressure more evenly than the general pipeline.The coefficient of variation of the sub-pipeline pressure was less than 5%.Wuyou 1179 hybrid rice was selected as the experimental object.The field seeding tests were carried out with different negative pressures and different numbers of groups of sucking holes as experimental factors.The results showed that the average qualified rate of seeding was 93.35%when only 2 groups of sucking holes were working under the seed sucking negative pressure of 2.0 kPa.The average qualified rate of seeding was 87.21%when 3 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The average qualified rate of seeding was 83.73%when 4 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The average qualified rate of seeding was 79.86%when 5 groups of sucking holes were working under the seed sucking negative pressure of 1.6 kPa.The main factors affecting the seeding effect were analyzed and the feasibility of a rice pneumatic seeder with adjustable seeding rates was verified by field tests.The research results can provide a basis for its practical production and application.
基金Supported by National Rice Industry Technology System(CARS-01-04A)National Key Technology Research and Development Program(2012BAD07B02)+1 种基金Ministry of Science and Technology’s Special Fund for Technology Development and Research of Research Institutes(2013EG134237)National Nonprofit Institute Research Grant of CATAS-TCGRI(2012RG005-3)~~
文摘In order to investigate the effects of seed rate on root twining power and seedling quality of machine-transplanted super rice cultivars, Yongyou 12 and Zhongzheyou No.l, total 6 seed rates were arranged, and the growth characteris- tics, quality and root twining power of machine-transplanted rice seedlings were studied. The results showed that seed rate had great effects on root twining power and qualityof rice seedlings; with the increase of seed rate, the root twining power of rice seedlings was increased, but the quality of rice seedlings was significantly reduced; under the condition of high seed rate, rice seedlings were weak with less accumulate dry matter and nonuniform growth, and the proportion of weak seedlings was higher. When the seed rate of both rice cultivars was reduced by 10 g/tray, the average seedling height was increased by 0.5 cm, leaf age was increased by 0.1, stem base diameter was increased by 0.1 cm, and root number per plant was increased by 0.3. When the seed rate was increased by 10 g/tray, the accumulated dry matter per plant was reduced by 5.1 mg. With the increase of seed rate, the seedling quality of Zhongzheyou No.1 was decreased rapidly, and the proportion of weak seedlings was increased rapidly. However, Yongyou 12 has a larger elasticity in seed rate. When the seed rate reached to 50 g/tray, blanket seedlings of Yongy- ou 12 were nursed with uniform growth and higher quality, but the shoots were sparse with lower leaf area index. However, when the seed rate was up to 50 g/tray, blanket seedlings of Zhongzheyou No.1 were nursed with uniform growth and higher quality, as well as higher-density shoots. From a comprehensive point of view, the optimum seed rates of Yongyou 12 and Zhongzheyou No.1 were 65 and 50 g/tray, respectively.
基金Supported by National Natural Science Foundation of China(31201181)Earmarked Fund for China Agriculture Research System(CARS-01-47)Project for Breeding and Demonstration of New Super Rice Varieties~~
文摘The seed setting rates of total 198 rice cultivars (lines) at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme natural high temperature on seed setting rate of different rice cultivar (line). The results showed that the contin- uous high temperature showed certain effects on the seed setting rates of tested materials, and significant differences were shown in seed setting rate among differ- ent rice cultivars (lines). The seed setting rates differed significantly among indica F1 hybrids derived from different sterile and restorer lines, indicating that the sterile and restorer lines had great effects on heat tolerances of different F~ hybrids. The cor- relation analysis showed that the seed setting rates of conventional indica restorer lines and conventional japonica rice cultivars (lines) were negatively related to the daily highest temperature (P〉0.05), and the seed setting rates of indica F1 hybrids were positively related to the seed setting rates of their restorer lines. Total four in- dica restorer lines, including Ninghuiguangkangzhan, Shuhui 527, Chenghui 3203 and Xianyin-8, and four new japonica rice cultivars (lines), including Wuyinjinghui (B2), Nanjing 4//W3660/Nanjing 44 (B12) and Wuyun 2330/JD6011 (B22) were pre- liminarily screened, and their seed setting rates were all close to the normal level (90%). The screened rice cultivars (lines) showed higher heat tolerances.
基金Supported by the Financial Genetic Engineering Program of Sichuan Province during the 12th Five-Year Plan Period(2011JYGC11-029)Sichuan Innovation Team Program of China Agriculture Research System+1 种基金Key Rice Breeding Technology Research and Development Program of Sichuan Province during the 12th Five-Year Plan Period(2011NZ0098-1)Key Science and Technology Program of Luzhou City(2011-N-09)~~
文摘[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps (control) were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation.
基金This study was supported by the Lee Foundation Scholarship(PhD)through International Rice Research Institute,the PhilippinesThe authors are thankful to Melencio J.APOSTOL for his technical assistance,and to Frederickson ENTILA and Evangelina S.ELLA for helpful suggestions and comments during this study.
文摘Rice(Oryza sativa)plants acquired excess photosynthates in the form of nonstructural carbohydrates(NSCs)in their stems and grain.Despite keen interest in rice NSC,the dynamics of NSC accumulation,translocation and re-accumulation have not yet been well investigated.AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds.Here we conducted three experiments,greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice(DSR)system,using elite lines incorporating AG1,AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On(AG1)and Ma-Zhan Red(AG2).In germinating seedlings,soluble sugars increased,while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil.Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage.But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017.Plant survival rate was positively correlated with the stem NSC at the early vegetative stage(21 days after sowing),and stem NSC was positively associated with plant height at different growth stages.Among the tested seeding rate,the most suitable seeding rate,4 g/m2 with shallow burial depth(0.5 cm),resulted in better seedling establishment,relatively higher seedling vigor index and higher leaf area index under flooding in DSR system.Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate,germination rate,and growth and biomass production.
基金funded by the Shandong Modern Agricultural Technology & Industry System,Chinathe Seed Production Technology and Development of Key Equipment and Demonstration(201203052) from Special Funds for Agro-scientific Research in the Public Interest,China+1 种基金the Maize Germplasm Innovation of Shandong Seed Industry Project,Chinathe Shandong Province Modern Agriculture Industrial Production Technology System,China (SDAIT-01-022-02)
文摘To explore the relation of maize grain texture and phenotypic traits with grain thin-layer drying rate,we observed the ultra-structure of maize grain,and tested three traits about the maize grain texture and four phenotypic traits.The vitreous part percentage was different(P〈0.05) among different maize inbred lines.There was a significant relationship between the drying rate with grain texture and phenotypic traits.Main factors that influenced the drying rate were different during different drying stages.New results observed that empirical constants(k and N) in drying equation were different for seed of the 30 inbred lines of maize.The k of simplified diffusion equation and N of page equation were significantly influenced by both grain texture and phenotypic traits.These results could be used as guideline parameters for drying maize seeds having different grain characteristics during different drying stages.
基金Supported by Agricultural Science and Technology Innovation Fund Project of Hunan Province(2023-CX17)Yuelushan Seed Industry Innovation Project of Hunan Innovation Province Construction Special Project(2021NK1012)。
文摘This study aimed to investigate the effects of treatments of rice pollen with different solvents on the seed setting rate.Rice pollen was treated with ultrapure water,0.5%dimethyl sulfoxide,ethanol(1%,5%,and 8%),and 0.5%dimethyl sulfoxide+8%ethanol,and the pollen without treatment was taken as the control.Then,crossing was conducted with the treated rice plants as the male parents and plants subjected to emasculation by warm water as the female parents.The seed setting rates of the male and female parents were then recorded.The results showed that the treatments with different solvents significantly decreased the seed setting rates of both male and female parents.The treatment with 1%ethanol resulted in the highest seed setting rates for both male and female parents,while the treatment with 0.5%dimethyl sulfoxide resulted in the lowest seed setting rates for both parents.Although these solvents affected the seed setting rate of rice,they served as intermediate solvents to dissolve chemicals used in the induction of rice haploids.This study layed a technical foundation for subsequent chemical induction of rice haploids.
基金the National Natural Science Foundation of China(39870506) Zhejiang Natural Science Foundation(397259).
文摘The influences of N application rate, timing, sowing date and seeding rate on β-glucan and protein content in barley grains were studied through the field experiments in Hangzhou, China during 1997 -2001. Protein content increased with N application rate and with N proportion applied at late stage. β-glucan content also responded significantly to N application rate and timing, but with different pattern with protein content. Of three N rate treatments, the medium N rate (135 kg ha-1) had the highest β-glucan content, being significantly higher than low N rate (90 kg ha-1) and no difference with high N rate (180 kg ha-1). A-mong three N timing treatments, two times of N top-dressing at both tillering and booting stage had significantly higher β-glucan content than once N top-dressing at tillering or booting stage. Sowing date has the dramatic effect on both β-glucan and protein content. Protein content decreased with the delayed sowing, and kernel weight showed opposite tendency. Either earlier or later sowing caused increased β-glucan content relative to sowing in early November, which is the normal date for barley sowing locally. Seeding rate had no significant influence on both β-glucan and protein content.
基金Supported by Special Project of GM(2009ZX08011-010B,2008XZ08011-011)~~
文摘[Objective] The paper was to explore the flowering habits and fertility of Gaozhou common wild rice (Oryza rufipogon Griff.) in Lingshui of Hainan Province. [Method] The flowering habits, pollen fertility and seed setting rate of Gaozhou common wild rice in Lingshui of Hainan Province were studied, and compared with the flowering habits of common wild rice in Hainan Province. [Result] Gaozhou common wild rice was able to flower twice a year. The first flowering stage was from mid-April to mid-June for about 60 d, and the daily peak flowering dates were from May 13 to May 22. The daily flowering time was mainly between 11:20 and 13:00, and the peak flowering was at 11:30. The second flowering stage was from late August to late October for about 45 d, and the peak flowering dates lasted from September 8 to September 18. The daily flowering time was mainly from 11:15 to 12:50, and the daily peak flowering was at 11:30. In both flowering stages, the panicles had the same flowering characteristics. The stigma exsertion reached a high value of 95.37% with single (46.23%) and dual (49.14%) stigmas exsertion respectively. A spikelet remained open for 1.5-2.5 h, the angle of spikelet opening varied from 29.9° to 32.4°. At the two flowering stage the average pollen fertility rates were 89.67% and 85.62%, the average seed setting rates were 58.81% and 55.79%, respectively. Hainan population flowered only once (from mid-October to mid-December), this had significant difference with the performance of Gaozhou common wild rice which flowered twice a year. [Conclusion] The results provided basis for the research on gene flow frequency from transgenic rice to wild rice and its relationship with flowering habits of common wild rice.
文摘When being planted in wide areas in southern China (23°23' - 33°23' N, 98°35' - 120°19' E, sea level above 2.7 - 1285 m), Liangyoupeijiu (LYPJ), a two-line hybrid rice combination, showed a seed setting rate of 75.2 - 77.2%, which was lower by 4.3 - 7.5 percent point than that of an indica hybrid rice Shanyou 63 (CK), with similar values of grain yield and coefficients of variation to CK. Sowing during 5-25th of May in Nanjing (32°3' N, 118°48' E), China, LYPJ headed before 4th September, and gave a seed setting rate of 75 - 90%, and grain yield over 1 kg/m2. If the sowing date was delayed to 14 - 15th, June, its heading date would be as late as 17th - 21st September, and seed setting rate would be declined by 10 - 15% in comparison with that on a suitable sowing date. When flowering took place at an average daily temperature range of 13.7 - 28.5℃ for five days, the spikelet fertility (SF) would be increased by 1.9- 10.7%, for each increment of 1℃. The suitable (SF≥90%) and safe (SF≥75%) temperatures for flowering stage were indicated to be 26.5℃ and 22.9℃, respectively. To get a high and stable seed set in cultivating LYPJ, it was recommended that LYPJ would be planted in the areas south of 34癗, and the favorable average daily temperature during flowering stage should be at 26 - 28℃.
基金supported by the National Natural Science Foundations of China(Grant No.30270814)the Teaching and Research Award Program for 0utstanding Young Teachers in Higher Education Institutions of M0E,P.R.China.
文摘The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice (Oryza sativa L.), including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy (WCLSM). Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and 'double set' of embryo sacs. The frequency of abnormal embryo sac in japonica (26.6%) was higher than that in indica (19.34%). In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties.
基金the National Natural Science Foundation of China (Grants Nos. 30070454 and 30471045)
文摘The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5-10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10-15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable.
基金Project(20476107) supported by the National Natural Science Foundation of ChinaProject(2005CB623702) supported by the National Basic Research Program of China
文摘Na4EDTA and EDTA were adopted as new additives to intensify the seeded precipitation process of sodium aluminate solution. The effects of the two additives at certain concentrations on the seeded precipitation rate of sodium aluminate solution, particle size distribution (PSD) and morphology of precipitated gibbsite were investigated using titration method, particle size analyzer and scanning electron microscope (SEM), respectively. The results show that the two additives can accelerate the seeded precipitation rate of sodium aluminate solution. At relatively high concentration, the facilitative effect of EDTA on sodium aluminate solution is more obvious than that of Na4EDTA. EDTA makes gibbsite particles thinner than Na4EDTA. The Na+ and H+ result in the different effects on the seeded precipitation rate of sodium aluminate solution in spite of the same EDTA anion in the two additives.
文摘The field experiment was conducted at Pulses Research Centre of Bangladesh Agricultural Institute (BARI), Ishurdi, Pabna, Bangladesh during rabi season of 2009 to find out the suitable management package for better plant establishment and higher yield of lentil under relay cropping. The experiment was based on three genotypes--Rajbari local, X95S-167(4) and X95S-136; two types of rice straw height--I 0 cm and 30 cm, three seed rates (kg/ha)--e.g., 30, 50 and 60, and three levels of fertilizer--control, 30-60-20 kg/ha of N-P2Os-K20 and 30-60-30 kg/ha of N-P2Os-KzO. The experiment was laid out in a split-split-split-plot design with three replications. Genotype was placed in the main-plot, rice straw height was in the sub-plot, seed rate was in the sub-sub-plot and fertilizer level was in the sub-sub-sub-plot. From this study, it was observed that lentil genotype X95S-136X planted with a rice straw height under 30 cm, at a seed rate of 50 kg/ha, and with a fertilizer level at 30-60-30 kg/ha of N-P2Os-K20 produced the highest lentil seed yield of 2,070 kg/ha and the lowest seed yield (550 kg/ha ) was obtained from genotype Rajbari local, planted with a rice straw height under 10 cm, at a seed rate of 30 kg/ha and no fertilizer. Similar result regarding seed and straw yield was also found in the farmer's field. The accession X95S-136 along with improved production technologies produced the highest grain and straw yield, and net return but the lowest grain and straw yield as well as net return were obtained by Rajbari local in the demonstration field. It was also identified that, by the inclusion of lentil in the transplanted monsoon rice field, lands could be brought under lentil cultivation to enhance lentil production, provide human nutrition and also to ensure soil health improvement for sustainable production system.
文摘Comparison of seed setting rate, plant height and Cheng's morphological traits were made between the F_2 progeny of indica-japonica hybrids, 02428/Pei'ai 64 and Taichung 65/Teqing. There existed resemblance normal distribution in plant height, and the distribution had no relationship with the seed setting rate and plant height in F_1 progeny, suggesting that there were no gametic selection for the plant height in the F_2 progeny of indica-japoniea hybrids. An average seed setting rate of the F_2 was equal to the F_1 populations in the recombination of 02428/Pei'ai 64 in which there was an excellent cross compatibility, but the remarkable increase in the seed setting rate from the F_1 to F_2 in Taichung 65/Teqing, indicating that gametic selection only took place in the indica-japonica crosses with low cross compatibility while null or little in the hybrids with high compatibility. There were many intermediate types among the leaf pubescence, color of hull at heading, and length of the first and second panicle internode, and these Cheng's morphological traits was distributed normally; there were also a little intermediate types in hull hairness and phenol reaction with bi-peak distribution; while length to width ratio of grain was inclined to the japonica types obviously showing a skewed distribution in the F2 population. Although Cheng's morphological traits in F_2 populations had inconsistent distribution types, Cheng's morphological traits index showed normal distribution, which suggested that intermediate types, i.e. indicalinous or japonicalinous, were primary in F_2 population.