时间频率传递的结果会受到非模型化误差和观测噪声的影响,其噪声常为高频信号,构建低通滤波器可在一定程度上消除观测值序列中的高频噪声信号.本文对Vondrak滤波函数的本质进行剖析,通过IGG3算法对钟差序列进行定权并采用频率响应法选...时间频率传递的结果会受到非模型化误差和观测噪声的影响,其噪声常为高频信号,构建低通滤波器可在一定程度上消除观测值序列中的高频噪声信号.本文对Vondrak滤波函数的本质进行剖析,通过IGG3算法对钟差序列进行定权并采用频率响应法选择适合的滤波因子;对不同的链路分别进行卫星双向时间频率传递(two-way satellite time and frequency transfer,TWSTFT)、基于软件接收机的卫星双向时间传递(two-way satellite time and frequency transfer based on software defined receiver,SDR-TWSTFT)和短基线共视时间频率传递实验,并对钟差结果采用抗差Vondrak滤波进行平滑去噪.结果表明:滤波后的钟差序列能够很好地反映原始钟差序列的趋势;平滑后的TWSTFT钟差结果,日波动效应得到了有效的抑制,精度有明显提升;对于共视钟差结果,精度有明显提升,与精密单点定位(precise point positioning,PPP)时间传递结果的差值保持在−1.0~1.0 ns范围内.展开更多
BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth fa...BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.展开更多
Recently, smoothed particle hydrodynamics (SPH) method has become popular in computational fluid dynamic and heat transfer simulation. The simplicity offered by this method made some complex system in physics such as ...Recently, smoothed particle hydrodynamics (SPH) method has become popular in computational fluid dynamic and heat transfer simulation. The simplicity offered by this method made some complex system in physics such as moving interface in multiphase flow, heat conductivity jumping in multiple material boundaries and many geometrical difficulties become relative easy to calculate. We will treat a relative easy example of melting process to test the method in solving fluid motion equation coupled by heat transfer process. The main heat transfer processes are caused by solid-liquid (medium to medium) heat diffusion and convection. System interaction with ambient temperature can be modeled by gas surrounding fluid-solid system. For the ambient temperature, we proposed surface particle heat transfer governed by convectional heat flux. Using local particle number density value as surface detection method, we applied cooling and heating to surface particle on the melting ice cube and water system. The simulation result is also verified by experiment.展开更多
微电网由并网运行模式转孤岛运行模式的平滑切换是微电网稳定运行研究的重要内容。为提高切换过程的暂态稳定性,针对风光储互补型微电网,在考虑有功功率缺额情况下,设计并提出了基于双馈感应风机有功功率综合控制模型的微电网平滑切换...微电网由并网运行模式转孤岛运行模式的平滑切换是微电网稳定运行研究的重要内容。为提高切换过程的暂态稳定性,针对风光储互补型微电网,在考虑有功功率缺额情况下,设计并提出了基于双馈感应风机有功功率综合控制模型的微电网平滑切换控制策略,利用风机和蓄电池的协调控制减小切换过程的暂态波动。该综合控制模型包括最大功率跟踪控制模块、虚拟惯性控制模块、转速恢复模块和转速保护模块。虚拟惯性控制和蓄电池的协调作用实现了微网运行模式的平滑切换,转速恢复模块和转速保护模块确保了双馈风机安全、稳定运行。基于DIg SILENT Power Factory软件搭建微网模型并进行仿真分析,验证了所提出控制策略的有效性。展开更多
文摘时间频率传递的结果会受到非模型化误差和观测噪声的影响,其噪声常为高频信号,构建低通滤波器可在一定程度上消除观测值序列中的高频噪声信号.本文对Vondrak滤波函数的本质进行剖析,通过IGG3算法对钟差序列进行定权并采用频率响应法选择适合的滤波因子;对不同的链路分别进行卫星双向时间频率传递(two-way satellite time and frequency transfer,TWSTFT)、基于软件接收机的卫星双向时间传递(two-way satellite time and frequency transfer based on software defined receiver,SDR-TWSTFT)和短基线共视时间频率传递实验,并对钟差结果采用抗差Vondrak滤波进行平滑去噪.结果表明:滤波后的钟差序列能够很好地反映原始钟差序列的趋势;平滑后的TWSTFT钟差结果,日波动效应得到了有效的抑制,精度有明显提升;对于共视钟差结果,精度有明显提升,与精密单点定位(precise point positioning,PPP)时间传递结果的差值保持在−1.0~1.0 ns范围内.
基金This study was supported by grants from the 973 National Basic ResearchProgram of China ( 2003CB515501 ) and the National Natural ScienceFoundation of China (No. 30270514).
文摘BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.
文摘Recently, smoothed particle hydrodynamics (SPH) method has become popular in computational fluid dynamic and heat transfer simulation. The simplicity offered by this method made some complex system in physics such as moving interface in multiphase flow, heat conductivity jumping in multiple material boundaries and many geometrical difficulties become relative easy to calculate. We will treat a relative easy example of melting process to test the method in solving fluid motion equation coupled by heat transfer process. The main heat transfer processes are caused by solid-liquid (medium to medium) heat diffusion and convection. System interaction with ambient temperature can be modeled by gas surrounding fluid-solid system. For the ambient temperature, we proposed surface particle heat transfer governed by convectional heat flux. Using local particle number density value as surface detection method, we applied cooling and heating to surface particle on the melting ice cube and water system. The simulation result is also verified by experiment.
文摘微电网由并网运行模式转孤岛运行模式的平滑切换是微电网稳定运行研究的重要内容。为提高切换过程的暂态稳定性,针对风光储互补型微电网,在考虑有功功率缺额情况下,设计并提出了基于双馈感应风机有功功率综合控制模型的微电网平滑切换控制策略,利用风机和蓄电池的协调控制减小切换过程的暂态波动。该综合控制模型包括最大功率跟踪控制模块、虚拟惯性控制模块、转速恢复模块和转速保护模块。虚拟惯性控制和蓄电池的协调作用实现了微网运行模式的平滑切换,转速恢复模块和转速保护模块确保了双馈风机安全、稳定运行。基于DIg SILENT Power Factory软件搭建微网模型并进行仿真分析,验证了所提出控制策略的有效性。
