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Stress/strain distributions for weld metal solidification crack instain less steels 被引量:2
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作者 魏艳红 刘仁培 +1 位作者 董祖珏 张海州 《China Welding》 EI CAS 2000年第1期38-43,共6页
This paper has simulated the driving force of solidification crack of stainless steels, that is, stress/strain field in the trail of molten pool. Firstly, the effect of the deformation in the molten pool was eliminate... This paper has simulated the driving force of solidification crack of stainless steels, that is, stress/strain field in the trail of molten pool. Firstly, the effect of the deformation in the molten pool was eliminated after the element rebirth method was adopted. Secondly, the influence of solidification shrinkage was taken into account by increasing thermal expansion coefficients of the steels at elevated temperatures. Finally, the stress/strain distributions of different conditions have been computed and analyzed. Furthermore, the driving force curves of the solidification crack of the steels have been obtained by converting strain time curves into strain temperature curves, which founds a basis for predicting welding solidification crack. 展开更多
关键词 welding solidification crack driving force of welding solidification crack stress/strain field numerical simulation
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INFLUENCE OF TOP- TEMPERATURE OF THERMAL CYCLE ON THERMAL STRESS/STRAIN AND THERMAL FATIGUE BEHAVIOR OF PURE IRON
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作者 J.H.Liu 1) and S.H.Jiang 1) and M.Yao 2) 1) Department of mechanical Engineering, Engineering college, Yangzhou University, Yangzhou 225009, China 2) Department of Material Science, Yanshan University, Qinhuangdao 066000, China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 1999年第5期852-855,共4页
By using a self-made thermal fatigue test machine of outer-constraint mode, the influence of top-temperature of thermal cycle T t on thermal stress-strain and thermal fatigue behavior of an industrial pure iron was in... By using a self-made thermal fatigue test machine of outer-constraint mode, the influence of top-temperature of thermal cycle T t on thermal stress-strain and thermal fatigue behavior of an industrial pure iron was investigated. The T t was varied from stress/strain 773K to 1073K. The results show that, increasing of T t , the thermal stress-strain cycles can be classified into four types, they are: compressive stress cycle; compressive strain-tensile stress cycle; compressive strain-tensile stress cycle than changing to compressive tensile plastic strain cycle; and finally, compressive tensile plastic strain cycle. It is also revealed that certain relationship does exist between thermal fatigue life and characteristics of thermal stress/strain cycle. When compressive tensile plastic strain cycle were appear by increasing of T t , thermal fatigue life decreasing rapidly. The concept of thermal fatigue transition temperature, and determining method were put up in this thesis. 展开更多
关键词 THERMAL FATIGUE THERMAL stress-strain
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Stress tensor determination by modified hydraulic tests on pre-existing fractures:Method and stress constraints
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作者 Guiyun Gao Chenghu Wang Ke Gao 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1637-1648,共12页
The hydraulic testing of pre-existing fractures(HTPF)is one of the most promising in situ stress measurement methods,particularly for three-dimensional stress tensor determination.However,the stress tensor determinati... The hydraulic testing of pre-existing fractures(HTPF)is one of the most promising in situ stress measurement methods,particularly for three-dimensional stress tensor determination.However,the stress tensor determination based on the HTPF method requires at least six tests or a minimum of 14-15 tests(under different conditions)for reliable results.In this study,we modified the HTPF method by considering the shear stress on each pre-existing fracture,which increased the number of equations for the stress tensor determination and decreased the number of tests required.Different shear stresses were attributed to different fractures by random sampling;therefore,the stress tensors were obtained by searching for the optimal solution using the least squares criterion based on the Monte Carlo method.Thereafter,we constrained the stress tensor based on the tensile strength criterion,compressive strength criterion,and vertical stress constraints.The inverted stress tensors were presented and analyzed based on the tensorial nature of the stress using the Euclidean mean stress tensor.Two stress-measurement campaigns in Weifang(Shandong Province,China)and Mercantour road tunnel(France)were implemented to highlight the validity and efficiency of the modified HTPF(M-HTPF)method.The results showed that the M-HTPF method can be applied for stress tensor inversion using only three to four tests on pre-existing fractures,neglecting the stress gradient.The inversion results were confined to relatively small distribution dispersions and were significantly reliable and stable due to the shear stresses on the fractures and the stress constraints employed.The M-HTPF method is highly feasible and efficient for complete stress tensor determination in a single borehole. 展开更多
关键词 stress tensor Hydraulic tests on pre-existing fractures Mean stress stress constraint Hydraulic fracturing
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A continuous and long-term in-situ stress measuring method based on fiber optic. Part I: Theory of inverse differential strain analysis
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作者 Kun-Peng Zhang Mian Chen +2 位作者 Chang-Jun Zhao Su Wang Yong-Dong Fan 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期1171-1189,共19页
A method for in-situ stress measurement via fiber optics was proposed. The method utilizes the relationship between rock mass elastic parameters and in-situ stress. The approach offers the advantage of long-term stres... A method for in-situ stress measurement via fiber optics was proposed. The method utilizes the relationship between rock mass elastic parameters and in-situ stress. The approach offers the advantage of long-term stress measurements with high spatial resolution and frequency, significantly enhancing the ability to measure in-situ stress. The sensing casing, spirally wrapped with fiber optic, is cemented into the formation to establish a formation sensing nerve. Injecting fluid into the casing generates strain disturbance, establishing the relationship between rock mass properties and treatment pressure.Moreover, an optimization algorithm is established to invert the elastic parameters of formation via fiber optic strains. In the first part of this paper series, we established the theoretical basis for the inverse differential strain analysis method for in-situ stress measurement, which was subsequently verified using an analytical model. This paper is the fundamental basis for the inverse differential strain analysis method. 展开更多
关键词 In-situ stress Fiber optic Orthotropic elastic Differential evolution ABAQUS
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Probabilistic-Ellipsoid Hybrid Reliability Multi-Material Topology Optimization Method Based on Stress Constraint
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作者 Zibin Mao Qinghai Zhao Liang Zhang 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第7期757-792,共36页
This paper proposes a multi-material topology optimization method based on the hybrid reliability of the probability-ellipsoid model with stress constraint for the stochastic uncertainty and epistemic uncertainty of m... This paper proposes a multi-material topology optimization method based on the hybrid reliability of the probability-ellipsoid model with stress constraint for the stochastic uncertainty and epistemic uncertainty of mechanical loads in optimization design.The probabilistic model is combined with the ellipsoidal model to describe the uncertainty of mechanical loads.The topology optimization formula is combined with the ordered solid isotropic material with penalization(ordered-SIMP)multi-material interpolation model.The stresses of all elements are integrated into a global stress measurement that approximates the maximum stress using the normalized p-norm function.Furthermore,the sequential optimization and reliability assessment(SORA)is applied to transform the original uncertainty optimization problem into an equivalent deterministic topology optimization(DTO)problem.Stochastic response surface and sparse grid technique are combined with SORA to get accurate information on the most probable failure point(MPP).In each cycle,the equivalent topology optimization formula is updated according to the MPP information obtained in the previous cycle.The adjoint variable method is used for deriving the sensitivity of the stress constraint and the moving asymptote method(MMA)is used to update design variables.Finally,the validity and feasibility of the method are verified by the numerical example of L-shape beam design,T-shape structure design,steering knuckle,and 3D T-shaped beam. 展开更多
关键词 stress constraint probabilistic-ellipsoid hybrid topology optimization reliability analysis multi-material design
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A novel binary effective medium model to describe the prepeak stressstrain relationship of combined bodies of rock-like material and rock 被引量:1
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作者 Tienan Wang Yue Zhai +2 位作者 Huan Gao Yubai Li Ruifeng Zhao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2023年第5期601-616,共16页
Combined bodies of rock-like material and rock are widely encountered in geotechnical engineering,such as tunnels and mines.The existing theoretical models describing the stress-strain relationship of a combined body ... Combined bodies of rock-like material and rock are widely encountered in geotechnical engineering,such as tunnels and mines.The existing theoretical models describing the stress-strain relationship of a combined body lack a binary feature.Based on effective medium theory,this paper presents the governing equation of the“elastic modulus”for combined and single bodies under triaxial compressive tests.A binary effective medium model is then established.Based on the compressive experiment of concretegranite combined bodies,the feasibility of determining the stress threshold based on crack axial strain is discussed,and the model is verified.The model is further extended to coal-rock combined bodies of more diverse types,and the variation laws of the compressive mechanical parameters are then discussed.The results show that the fitting accuracy of the model with the experimental curves of the concretegranite combined bodies and various types of coal-rock combined bodies are over 95%.The crack axial strain method can replace the crack volumetric strain method,which clarifies the physical meanings of the model parameters.The variation laws of matrix parameters and crack parameters are discussed in depth and are expected to be more widely used in geotechnical engineering. 展开更多
关键词 Combined body stress-strain relationship Hooke’s law Effective medium theory stress threshold determination
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Effects of intermediate stress on deep rock strainbursts under true triaxial stresses 被引量:1
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作者 Lihua Hu Liyuan Yu +2 位作者 Minghe Ju Xiaozhao Li Chun’an Tang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第3期659-682,共24页
The effect of intermediate stress(in situ tunnel axial)on a strainburst is studied with a threedimensional(3D)bonded block distinct element method(DEM).A series of simulations of strainbursts under true triaxial in si... The effect of intermediate stress(in situ tunnel axial)on a strainburst is studied with a threedimensional(3D)bonded block distinct element method(DEM).A series of simulations of strainbursts under true triaxial in situ stress conditions(i.e.high tangential stress,moderate intermediate stress and low radial stress)of near-boundary rock masses are performed.Compared with the experimental results,the DEM model is able to capture the stress-strain response,failure pattern and energy balance of strainbursts.The fracturing processes of strainbursts are also numerically reproduced.Numerical results show that,as the intermediate stress increases:(1)The peak strain of strainbursts increases,the yield stress increases,the rock strength increases linearly,and the ratio of yield stress to rock strength decreases,indicating that the precursory information on strainbursts is enhanced;(2)Tensile and shear cracks increase significantly,and slabbing and bending of rock plates are more pronounced;and(3)The stored elastic strain energy and dissipated energy increase linearly,whereas the kinetic energy of the ejected rock fragments increases approximately exponentially,implying an increase in strainburst intensity.By comparing the experimental and numerical results,the effect of intermediate stress on the rock strength of strainbursts is discussed in order to address three key issues.Then,the Mogi criterion is applied to construct new strength criteria for strainbursts by converting the one-face free true triaxial stress state of a strainburst to its equivalent true triaxial stress state.In summary,the effect of intermediate stress on strainbursts is a double-edged sword that can enhance the rock strength and the precursory information of a strainburst,but also increase its intensity. 展开更多
关键词 strainbursts Intermediate stress Distinct element method(DEM) Structural failure True triaxial Strength criterion
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Two-dimensional plane strain consolidation of unsaturated soils considering the depth-dependent stress
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作者 Lei Wang Sidong Shen +2 位作者 Tianyi Li Minjie Wen Annan Zhou 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第6期1603-1614,共12页
In practical engineering,the total vertical stress in the soil layer is not constant due to stress diffusion,and varies with time and depth.Therefore,the purpose of this paper is to investigate the effect of stress di... In practical engineering,the total vertical stress in the soil layer is not constant due to stress diffusion,and varies with time and depth.Therefore,the purpose of this paper is to investigate the effect of stress diffusion on the two-dimensional(2D)plane strain consolidation properties of unsaturated soils when the stress varies with time and depth.A series of semi-analytical solutions in terms of excess pore air and water pressures and settlement for 2D plane strain consolidation of unsaturated soils can be derived with the joint use of Laplace transform and Fourier sine series expansion.Then,the inverse Laplace transform of the semi-analytical solution is given in the time domain using a self-programmed code based on Crump’s method.The reliability of the obtained solutions is proved by the degeneration.Finally,the 2D plots of excess pore pressures and the curves of settlement varying with time,considering different physical parameters of unsaturated soil stratum and depth-dependent stress,are depicted and analyzed to study the 2D plane strain consolidation properties of unsaturated soils subjected to the depthdependent stress. 展开更多
关键词 Semi-analytical solutions Two-dimensional(2D)plane strain CONSOLIDATION Unsaturated soils Depth-dependent stress Laplace transform
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Formulation and procedure for in situ stress back-analysis from borehole strain changes measured during nearby underground excavation
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作者 Cui Lin D.H.Steve Zou 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2023年第8期1931-1943,共13页
Estimation of in situ stresses based on back-analysis of measured stress changes and displacements has become an alternative to the direct stress measurement methods.In order to help users conduct own measurement and ... Estimation of in situ stresses based on back-analysis of measured stress changes and displacements has become an alternative to the direct stress measurement methods.In order to help users conduct own measurement and analysis,this paper presents in detail a field stress back-analysis approach directly from borehole strain changes measured during nearby underground excavation.Essential formulations in major steps and the procedure for the entire analysis process are provided to allow users to follow.The instrument for borehole strain change measurement can be the CSIR or CSIRO stress cells and other borehole strain cells that can measure strains on borehole walls.Strain changes corresponding to the stress changes at a borehole location are calculated in borehole environment.The stress changes due to nearby excavation can be calculated by an analytical model for a single circular opening and simulated by a numerical model for non-circular and multiple openings.These models are based on isotropic,homogeneous and linear elastic assumptions.The analysis of borehole strain changes is accomplished by multiple linear regression based on error minimization and an integrated process provides the best-fit solution directly to the in situ stresses.A statistical technique is adopted for screening outliers in the measurement data,checking measurement compatibility and evaluating the reliability of analysis results.An application example is included to demonstrate the practical application and the analysis procedure. 展开更多
关键词 In situ stress field BACK-ANALYSIS Borehole strain change Multiple linear regression Underground excavation Data compatibility Result reliability
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Ferroptosis and endoplasmic reticulum stress in ischemic stroke 被引量:4
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作者 Yina Li Mingyang Li +4 位作者 Shi Feng Qingxue Xu Xu Zhang Xiaoxing Xiong Lijuan Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期611-618,共8页
Ferroptosis is a form of non-apoptotic programmed cell death,and its mechanisms mainly involve the accumulation of lipid peroxides,imbalance in the amino acid antioxidant system,and disordered iron metabolism.The prim... Ferroptosis is a form of non-apoptotic programmed cell death,and its mechanisms mainly involve the accumulation of lipid peroxides,imbalance in the amino acid antioxidant system,and disordered iron metabolism.The primary organelle responsible for coordinating external challenges and internal cell demands is the endoplasmic reticulum,and the progression of inflammatory diseases can trigger endoplasmic reticulum stress.Evidence has suggested that ferroptosis may share pathways or interact with endoplasmic reticulum stress in many diseases and plays a role in cell survival.Ferroptosis and endoplasmic reticulum stress may occur after ischemic stroke.However,there are few reports on the interactions of ferroptosis and endoplasmic reticulum stress with ischemic stroke.This review summarized the recent research on the relationships between ferroptosis and endoplasmic reticulum stress and ischemic stroke,aiming to provide a reference for developing treatments for ischemic stroke. 展开更多
关键词 cell death endoplasmic reticulum stress ferroptosis ischemic stroke lipid peroxidation
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Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress 被引量:1
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作者 Hao Liang Qiling Shi +8 位作者 Xing Li Peipei Gao Daling Feng Xiaomeng Zhang Yin Lu Jingsen Yan Shuxing Shen Jianjun Zhao Wei Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期461-472,共12页
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm... Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress. 展开更多
关键词 Chinese cabbage Salt stress Carbon metabolism PHOTOSYNTHESIS CHLOROPLAST
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A semi-analytical model for coupled flow in stress-sensitive multi-scale shale reservoirs with fractal characteristics 被引量:1
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作者 Qian Zhang Wen-Dong Wang +4 位作者 Yu-Liang Su Wei Chen Zheng-Dong Lei Lei Li Yong-Mao Hao 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期327-342,共16页
A large number of nanopores and complex fracture structures in shale reservoirs results in multi-scale flow of oil. With the development of shale oil reservoirs, the permeability of multi-scale media undergoes changes... A large number of nanopores and complex fracture structures in shale reservoirs results in multi-scale flow of oil. With the development of shale oil reservoirs, the permeability of multi-scale media undergoes changes due to stress sensitivity, which plays a crucial role in controlling pressure propagation and oil flow. This paper proposes a multi-scale coupled flow mathematical model of matrix nanopores, induced fractures, and hydraulic fractures. In this model, the micro-scale effects of shale oil flow in fractal nanopores, fractal induced fracture network, and stress sensitivity of multi-scale media are considered. We solved the model iteratively using Pedrosa transform, semi-analytic Segmented Bessel function, Laplace transform. The results of this model exhibit good agreement with the numerical solution and field production data, confirming the high accuracy of the model. As well, the influence of stress sensitivity on permeability, pressure and production is analyzed. It is shown that the permeability and production decrease significantly when induced fractures are weakly supported. Closed induced fractures can inhibit interporosity flow in the stimulated reservoir volume (SRV). It has been shown in sensitivity analysis that hydraulic fractures are beneficial to early production, and induced fractures in SRV are beneficial to middle production. The model can characterize multi-scale flow characteristics of shale oil, providing theoretical guidance for rapid productivity evaluation. 展开更多
关键词 Multi-scale coupled flow stress sensitivity Shale oil Micro-scale effect Fractal theory
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High-pressure and high-temperature sintering of pure cubic silicon carbide:A study on stress-strain and densification
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作者 刘金鑫 彭放 +5 位作者 马国龙 梁文嘉 何瑞琦 管诗雪 唐越 向晓君 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第9期498-505,共8页
Silicon carbide(SiC)is a high-performance structural ceramic material with excellent comprehensive properties,and is unmatched by metals and other structural materials.In this paper,raw SiC powder with an average grai... Silicon carbide(SiC)is a high-performance structural ceramic material with excellent comprehensive properties,and is unmatched by metals and other structural materials.In this paper,raw SiC powder with an average grain size of 5μm was sintered by an isothermal-compression process at 5.0 GPa and 1500?C;the maximum hardness of the sintered samples was31.3 GPa.Subsequently,scanning electron microscopy was used to observe the microscopic morphology of the recovered SiC samples treated in a temperature and extended pressure range of 0-1500?C and 0-16.0 GPa,respectively.Defects and plastic deformation in the SiC grains were further analyzed by transmission electron microscopy.Further,high-pressure in situ synchrotron radiation x-ray diffraction was used to study the intergranular stress distribution and yield strength under non-hydrostatic compression.This study provides a new viewpoint for the sintering of pure phase micron-sized SiC particles. 展开更多
关键词 high pressure and high temperature silicon carbide stress analysis DEFECT
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The role of strain in oxygen evolution reaction
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作者 Zihang Feng Chuanlin Dai +5 位作者 Zhe Zhang Xuefei Lei Wenning Mu Rui Guo Xuanwen Liu Junhua You 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第6期322-344,I0009,共24页
The oxygen evolution reaction(OER)is a crucial step in metal-air batteries and water splitting technologies,playing a significant role in the efficiency and achievable heights of these two technologies.However,the OER... The oxygen evolution reaction(OER)is a crucial step in metal-air batteries and water splitting technologies,playing a significant role in the efficiency and achievable heights of these two technologies.However,the OER is a four-step,four-electron reaction,and its slow kinetics result in high overpotentials,posing a challenge.To address this issue,numerous strategies involving modified catalysts have been proposed and proven to be highly efficient.In these strategies,the introduction of strain has been widely reported because it is generally believed to effectively regulate the electronic structure of metal sites and alter the adsorption energy of catalyst surfaces with reaction intermediates.However,strain has many other effects that are not well known,making it an important yet unexplored area.Based on this,this review provides a detailed introduction to the various roles of strain in OER.To better explain these roles,the review also presents the definition of strain and elucidates the potential mechanisms of strain in OER based on the d-band center theory and adsorption volcano plot.Additionally,the review showcases various ways of introducing strain in OER through examples reported in the latest literature,aiming to provide a comprehensive perspective for the development of strain engineering.Finally,the review analyzes the appropriate proportion of strain introduction,compares compressive and tensile strain,and examines the impact of strain on stability.And the review offers prospects for future research directions in this emerging field. 展开更多
关键词 Oxygen evolution reaction strain generation Tensile strain Compressive strain strain mechanism strain effects
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Tensile Fractures and in situ Stress Measurement Data Constraints on Cretaceous-Present Tectonic Stress Field Evolution of the Tanlu Fault Zone in Shandong Province,North China Craton
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作者 YANG Chengwei WANG Chenghu 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2023年第6期1616-1624,共9页
Tectonic stress fields are the key drivers of tectonic events and the evolution of regional structures.The tectonic stress field evolution of the Tanlu fault zone in Shandong Province,located in the east of the North ... Tectonic stress fields are the key drivers of tectonic events and the evolution of regional structures.The tectonic stress field evolution of the Tanlu fault zone in Shandong Province,located in the east of the North China Craton(NCC),may have preserved records of the NCC’s tectonic history.Borehole television survey and hydraulic fracturing were conducted to analyze the paleo and present tectonic stress fields.Three groups of tensile fractures were identified via borehole television,their azimuths being NNW-SSE,NW-SE and NE-SW,representing multiple stages of tectonic events.Hydraulic fracturing data indicates that the study region is experiencing NEE-SWW-oriented compression and nearly-N-Soriented extension,in accordance with strike-slip and compression.Since the Cretaceous,the orientation of the extensional stress has evolved counterclockwise and sequentially from nearly-NW-SE-oriented to NE-SW-oriented and even nearly N-S-oriented,the stress state having transitioned from strike-slip-extension to strike-slip-compression,in association with the rotating and oblique subduction of the Pacific Plate beneath the NCC,with the participation of the Indian Plate. 展开更多
关键词 borehole television tectonic stress field hydraulic fracturing Tanlu fault zone North China Craton
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Frequency Domain Fatigue Evaluation on SCR Girth-Weld Based on Structural Stress 被引量:1
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作者 ZHANG Long ZHAO Tian-feng 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期255-270,共16页
The Steel Catenary Riser(SCR)is a vital component for transporting oil and gas from the seabed to the floating platform.The harsh environmental conditions and complex platform motion make the SCR’s girth-weld prone t... The Steel Catenary Riser(SCR)is a vital component for transporting oil and gas from the seabed to the floating platform.The harsh environmental conditions and complex platform motion make the SCR’s girth-weld prone to fatigue failure.The structural stress fatigue theory and Master S-N curve method provide accurate predictions for the fatigue damage on the welded joints,which demonstrate significant potential and compatibility in multi-axial and random fatigue evaluation.Here,we propose a new frequency fatigue model subjected to welded joints of SCR under multiaxial stress,which fully integrates the mesh-insensitive structural stress and frequency domain random process and transforms the conventional welding fatigue technique of SCR into a spectrum analysis technique utilizing structural stress.Besides,a full-scale FE model of SCR with welds is established to obtain the modal structural stress of the girth weld and the frequency response function(FRF)of modal coordinate,and a biaxial fatigue evaluation about the girth weld of the SCR can be achieved by taking the effects of multi-load correlation and pipe-soil interaction into account.The research results indicate that the frequency-domain fatigue results are aligned with the time-domain results,meeting the fatigue evaluation requirements of the SCR. 展开更多
关键词 SCR girth weld random vibration self(cross)power spectrum structural stress method biaxial fatigue damage
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Involvement of the ABA-and H_(2)O_(2)-Mediated Ascorbate-Glutathione Cycle in the Drought Stress Responses of Wheat Roots 被引量:1
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作者 Mengyuan Li Zhongye Gao +2 位作者 Lina Jiang Leishan Chen Jianhui Ma 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第2期329-342,共14页
Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and th... Abscisic acid(ABA),hydrogen peroxide(H_(2)O_(2)) and ascorbate(AsA)–glutathione(GSH)cycle are widely known for their participation in various stresses.However,the relationship between ABA and H_(2)O_(2) levels and the AsA–GSH cycle under drought stress in wheat has not been studied.In this study,a hydroponic experiment was conducted in wheat seedlings subjected to 15%polyethylene glycol(PEG)6000–induced dehydration.Drought stress caused the rapid accumulation of endogenous ABA and H_(2)O_(2) and significantly decreased the number of root tips compared with the control.The application of ABA significantly increased the number of root tips,whereas the application of H_(2)O_(2) markedly reduced the number of root tips,compared with that under 15%PEG-6000.In addition,drought stress markedly increased the DHA,GSH and GSSG levels,but decreased the AsA levels,AsA/DHA and GSH/GSSG ratios compared with those in the control.The activities of the four enzymes in the AsA–GSH cycle were also markedly increased under drought stress,including glutathione reductase(GR),ascorbate peroxidase(APX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate reductase(DHAR),compared with those in the control.However,the application of an ABA inhibitor significantly inhibited GR,DHAR and APX activities,whereas the application of an H_(2)O_(2) inhibitor significantly inhibited DHAR and MDHAR activities.Furthermore,the application of ABA inhibitor significantly promoted the increases of H_(2)O_(2) and the application of H_(2)O_(2) inhibitor significantly blocked the increases of ABA,compared with those under 15% PEG-6000.Taken together,the results indicated that ABA and H_(2)O_(2) probably interact under drought stress in wheat;and both of them can mediate drought stress by modulating the enzymes in AsA–GSH cycle,where ABA acts as the main regulator of GR,DHAR,and APX activities,and H_(2)O_(2) acts as the main regulator of DHAR and MDHAR activities. 展开更多
关键词 ABA H_(2)O_(2) AsA-GSH cycle drought stress wheat roots
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Identification of novel salt stress-responsive microRNAs through sequencing and bioinformatic analysis in a unique halophilic Dunaliella salina strain
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作者 Fan GAO Fangru NAN +4 位作者 Jia FENG Junping LÜ Qi LIU Xudong LIU Shulian XIE 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第4期1558-1574,共17页
Dunaliella salina is a classic halophilic alga.However,its molecular mechanisms in response to high salinity at the post transcriptional level remain unknown.A unique halophilic alga strain,DS-CN1,was screened from fo... Dunaliella salina is a classic halophilic alga.However,its molecular mechanisms in response to high salinity at the post transcriptional level remain unknown.A unique halophilic alga strain,DS-CN1,was screened from four D.salina strains via cell biological,physiological,and biochemical methods.High-throughput sequencing of small RNAs(sRNAs)of DS-CN1 in culture medium containing 3.42-mol/L NaCl(SS group)or 0.05-mol/L NaCl(CO group)was performed on the BGISEQ-500 platform.The annotation and sequences of D.salina sRNAs were profiled.Altogether,44 novel salt stress-responsive microRNAs(miRNAs)with a relatively high C content,with the majority of them being 24 nt in length,were identified and characterized in DS-CN1.Twenty-one differentially expressed miRNAs(DEMs)in SS and CO were screened via bioinformatic analysis.A total of 319 putative salt stress-related genes targeted(104 overlapping genes)by novel miRNAs in this alga were screened based on our previous transcriptome sequencing research.Furthermore,these target genes were classified and enriched by GO and KEGG pathway analysis.Moreover,5 novel DEMs(dsa-mir3,dsa-mir16,dsa-mir17,and dsa-mir26 were significantly upregulated,and dsa-mir40 was significantly downregulated)and their corresponding 10 target genes involved in the 6 significantly enriched metabolic pathways were verified by quantitative real-time PCR.Next,their regulatory relationships were comprehensively analyzed.Lastly,a unique salt stress response metabolic network was constructed based on the novel DEM-target gene pairs.Taken together,our results suggest that 44 novel salt stress-responsive microRNAs were identified,and 4 of them might play important roles in D.salina upon salinity stress and contribute to clarify its distinctive halophilic feature.Our study will shed light on the regulatory mechanisms of salt stress responses. 展开更多
关键词 Dunaliella salina salt stress response small RNA(sRNAs)sequencing microRNA(miRNAs)
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Simulating the Stress-Strain Response of Building under Oscillating Wind Conditions
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作者 Miao Liu Zijie Qu 《Open Journal of Civil Engineering》 2023年第3期443-453,共11页
Wind load is one of the main lateral control loads that need to be considered in the design of high-rise building structures. It is also of great engineering significance to study the influence of static wind load or ... Wind load is one of the main lateral control loads that need to be considered in the design of high-rise building structures. It is also of great engineering significance to study the influence of static wind load or time-varying wind load on the dynamic response of structures. In this paper, a high-rise building with a rectangular section (46.8 m × 27 m × 33 m) is simulated based on Ansys18.0 APDL software. The real situation of its response under no lateral wind load and different fluctuating wind load conditions is simulated and the stress and strain response of the building under steady-state and time-varying wind load is given. The results show that the upper strain of the structure under wind load is about 1/1000 of the bottom strain, and the strain of the structure shows obvious accumulation from the top to the bottom, that is, the bottom strain of the building will be higher than the top strain. The influence of time-varying wind load on building structure is related to the loading position of wind load on the structure. The results provide a basis for the structural wind resistance design of this type of building. 展开更多
关键词 Multistory Framework Cumulative strain Wind Load Analysis Finite Element Tool
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Heat stress induced hepatocyte apoptosis in largemouth bass Micropterus salmoides via IRE1α/TRAF2/ASK1/JNK pathway
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作者 Xuqian ZHAO Wenjia MAO +1 位作者 Zijie LIN Qufei LING 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第3期988-1000,共13页
Heat stress(HS)has been shown to adversely affect fish livers and can lead to extensive apoptosis.To investigate the relationship between endoplasmic reticulum(ER)stress and HS-induced apoptosis in fish livers,we isol... Heat stress(HS)has been shown to adversely affect fish livers and can lead to extensive apoptosis.To investigate the relationship between endoplasmic reticulum(ER)stress and HS-induced apoptosis in fish livers,we isolated and cultured primary hepatocytes of largemouth bass,Micropterus salmoides by trypsin method,then established an in-vitro model of liver cells under HS(35℃).The contents of lactic dehydrogenase(LDH)and hydrogen peroxide(H2O2)were determined to evaluate the effects of HS on hepatocyte injury and oxidative stress.RT-qPCR was performed to discover the key genes in unfolded protein response(UPR)pathways involved at different HS duration.ERS inhibitor 4-PBA and IRE1αinhibitor 4μ8C were used to further investigate the effects of HS on IRE1αapoptosis pathway in hepatocytes.Results show that HS led to significant increases in the release of LDH,the content of H2O2,and the expressions of oxidative protein folding genes(ero1αand pdi)under HS,suggesting severe hepatocyte injury and oxidative stress happened in heat-stressed largemouth bass hepatocytes.The continuous activation of IRE1αpathway genes(grp78,grp94,atf6,perk,eif2a,atf4,chop,ire1α,traf2,ask1,jnk1,and jnk2)indicated that HS led significantly to ER stress.In particular,the mRNA expression levels of ER stress-related genes(grp78,grp94,atf6,perk,ire1α,chop,jnk1,and jnk2)in the high temperature(HT)+4-PBA group and the HT+4μ8C group were significantly down-regulated under HS.After 4μ8C treatment,the expression levels of apoptosis-related genes(caspase-2,caspase-3,caspase-6,caspase-7,caspase-8,caspase-9,and caspase-10)and LDH content were significantly decreased,whereas the cell survival rate was significantly increased when given 4-PBA or 4μ8C treatment.These findings demonstrate that HS could induce liver apoptosis of largemouth bass through the IRE1αpathway,which may act as a key switch mediating liver apoptosis of largemouth bass under HS. 展开更多
关键词 heat stress Micropterus salmoides endoplasmic reticulum stress APOPTOSIS oxidative stress
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