Analysis of Occurrence and Mixed Infection of Sugarcane Bacilliform Virus Disease in Hainan Sugarcane-growing Area

[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 sugarcane-growing areas in Hainan Province.Molecular detection of sugarcane bacilliform virus(SCBV)was carried out by PCR using specific primers.[Results]SCBV was detected in 244 out of 348 sugarcane samples,with an average detection rate of 70.11%.The highest detection rate was 76.66%in the Danzhou sugarcane-growing area,while the lowest was 57.14%in the Baisha sugarcane-growing area.The SCBV-positive samples were subjected to testing for SCYLV,SCSMV,SrMV,and SCMV,respectively.The results indicated that 106 out of 244 positive samples exhibited a single infection with SCBV,while 138 samples exhibited mixed infections with SCBV and other sugarcane viruses.The proportion of mixed infections among the SCBV-positive samples was as high as 56.56%.Among the various types of mixed infections,two-virus and three-virus mixed infections were the most prevalent.[Conclusions]SCBV has emerged as a significant threat to the secure production of sugarcane in the Hainan sugarcane-growing region.It presents an explosive infection in the Hainan sugarcane-growing region and frequently combines with other sugarcane viruses to infect sugarcane.The findings of this study will provide a theoretical foundation for the prevention and control of sugarcane bacilliform virus disease.

A growth-regulating factor 7(GRF7)-mediated gene regulatory network promotes leaf growth and expansion in sugarcane

Knowledge of the function of growth-regulating factors(GRFs)in sugarcane(Saccharum officinarum and S.spontaneum)growth and development could assist breeders in selecting desirable plant architectures.However,limited information about GRFs is available in Saccharum due to their polyploidy.In this study,22 GRFs were identified in the two species and their conserved domains,gene structures,chromosome location,and synteny were characterized.GRF7 expression varied among tissues and responded to diurnal rhythm.SsGRF7-YFP was localized preferentially in the nucleus and appears to act as a transcriptional cofactor.SsGRF7 positively regulated the size and length of rice leaves,possibly by regulating cell size and plant hormones.Of seven potential transcription factors binding to the SsGRF7 promoter in S.spontaneum,four showed positive expression patterns,and two showed negative expression patterns relative to SsGRF7.

Sugarcane leaves-derived polyphenols alleviate metabolic syndrome and modulate gut microbiota of ob/ob mice

Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in mice.In the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob mice.Notably,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides acidifaciens.Oral gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese mice.These results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.

Chemically Modified Sugarcane Bagasse for Innovative Bio-Composites. Part One: Production and Physico-Mechanical Properties

Sugarcane bagasse is an agro-waste that could replace timber resources for the production of bio-composites.Composite boards such as particleboard offer an issue for the use and recycling of poor quality timber,and these engineered products can overcome some solid wood limitations such as heterogeneity and dimension.Bagasse offers an alternative to wood chips for particleboard production but present some disadvantages as well,such as poor physico-mechanical properties.To address these issues,bagassefibers were treated with an innovative natural resin formulated with tannin and furfural.Impregnated particles with different concentrations of resin(5%,10%,and 15%m/m)were exposed to temperatures of 40°C,60°C,80°C,and 100°C for resin curing.Various types of tannin-based adhesives,including tannin formaldehyde,tannin/formaldehyde-furfural,and tannin hex-amine,were utilized for bonding the treated bagasse particles.The resultant panels were assessed for their physical and mechanical properties and compared to those produced using Melamine-Urea-Formaldehyde(MUF)adhe-sive.The density of the panels varied from 650 to 730 kg/m3 depending on the resin concentration.The values for both modulus of elasticity and modulus of rupture increased as the resin concentration increased.The internal bonding values exhibited an increase with resin concentration up to a critical point,after which a decreasing trend was observed.The water absorption and thickness swelling were significantly reduced with an increase in resin concentration.However,the panels produced using MUF adhesive yielded the most favorable physico-mechanical properties.Additionally,the panels made with tannin-based adhesives met the minimum requirements specified in the standard EN 312(specifications for uncoated resin-bonded particleboards)for application in dry condi-tions.The analysis of formaldehyde emissions indicated that panels produced with tannin-based adhesives exhib-ited significantly lower emissions compared to those made with MUF.The tannin/furfural resin showed great potential for improving the quality of bagasse particleboard using tannin-based adhesives.

Assessment of Physico-Chemical and Microbial Quality of Sugarcane Juice Sold by Street Vendors in Three Regions of Bangladesh

Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, PH, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, PH, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 105 - 43.6 × 105 cfu/ml, 4.6 × 105 - 21.6 × 105 cfu/ml and 3.6 × 105 - 36.6 × 105 cfu/ml respectively, where the permitted value is 1.0 × 104 cfu/ml, whereas the total coliform count was ranged from 0.4 × 105 - 6.4 × 105 cfu/ml, 0.6 × 105 - 8.4 × 105 cfu/ml and 0.00 - 8.4 × 105 cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 105 - 56.5 × 105 cfu/ml, 21.5 × 105 - 54.5 × 105 cfu/ml and 32.5 × 105 - 68.5 × 105 cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.

Establishment and Application of Prevention and Control Techniques of New Sugarcane White Leaf Disease

Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10 years of collaborative research,and achieved several innovative achievements.SCWL phytoplasmas newly recorded in China and the new subgroup of SCWL phytoplasmas(16SrXI-D)were discovered for the first time in Yunnan,and the whole genome analysis of the epidemic subgroup was completed.The main transmission source of SCWL pathogens has been identified as infected seed canes,and Tettigoniella viridis and Clovia conifer were newly discovered as vectors for virus transmission.The disease resistance of 25 main varieties was identified,and 10 control varieties were selected.The prevention and control strategy of"emphasizing early warning,strictly carrying out quarantine,blocking the vectors and controlling residual plants"was put forward,and a comprehensive prevention technique was established through integration of various techniques,and standardized technical regulations were formulated for demonstration application.The promotion and application of these achievements have realized scientific prevention and control of SCWL,effectively curbed the spread of SCWL,and ensured the safety of sugarcane producing areas in China,achieving great economic,social and ecological benefits and providing technical support for high-quality development,loss reduction and efficiency improvement of China's sugar industry.

Past and recent advances in sugarcane cytogenetics

The Saccharum genus comprises species with large and variable chromosome numbers, leading to challenges in genomic studies and breeding improvement. Cytogenetics, including classical and molecular approaches, has played a central role in deciphering the genome structure, classification, and evolution of the genus Saccharum. The application of fluorescence in situ hybridization using oligonucleotide probes significantly improved our understanding of the complex genomes of Saccharum species. This paper reviews the application and progress of cytogenetic techniques in Saccharum. Future applications of cytogenetics are discussed, as they could benefit both genomic studies and breeding of sugarcane as well as other plants with complex genomes.

Overexpression of a sugarcane ScCaM gene negatively regulates salinity and drought stress responses in transgenic Arabidopsis thaliana

Calmodulin(CaM)proteins play a key role in signal transduction under various stresses.In the present study,the effects of a sugarcane ScCaM gene(NCBI accession number:GQ246454)on drought and salt stress tolerance in transgenic Arabidopsis thaliana and Escherichia coli cells were evaluated.The results demonstrated a significant negative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A.thaliana,as indicated by the phenotypes.In addition,the expression of AtP5CS and AtRD29A,two genes tightly related to stress resistance,was significantly lower in the overexpression lines than in the wild type.The growth of E.coli BL21 cells expressing ScCaM showed weaker tolerance under mannitol and NaCl stress.Taken together,this study revealed that the ScCaM gene plays a negative regulatory role in both mannitol and NaCl stresses,and it possibly exerts protective mechanisms common in both prokaryotes and eukaryotes under stress conditions.

Establishment and Application of Identification Methods for Resistance to Sugarcane White Leaf Disease(SCWL)

[Objectives]This study was conducted to establish simple, efficient, stable, standardized and practical identification methods for sugarcane resistance to white leaf disease(SCWL), and promote the breeding for sugarcane resistance to SCWL. [Methods]The identification technology of sugarcane resistance to SCWL was systematically studied and explored from the aspects of sugarcane material treatment and planting, inoculation liquid preparation, inoculation method, disease investigation, grading standard formulation, etc., and two sets of simple, efficient, stable, standardized and practical accurate identification methods for sugarcane resistance to SCWL were created for the first time, namely, the seed cane coating inoculation method and the stem-cutting inoculation method at the growth stage. The seed cane coating inoculation method includes the steps of directly screening SCWL phytoplasma, extracting juice from cane and adding 10 times of sterile water to prepare an inoculation liquid, spraying seed cane on plastic film to keep moisture, planting the inoculated materials in barrels in an insect-proof greenhouse for cultivation, investigating the incidence rate 30 d after inoculation, and evaluating the disease resistance according to the 1-5 level standard. The method of stem-cutting inoculation includes the steps of directly screening sugarcane stems carrying SCWL phytoplasma and adding 10 times of sterile water to prepare an inoculation liquid, cultivating the identification materials in an insect-proof greenhouse, dropping 100 μl of the inoculation liquid into each root incision with a pipette gun at the age of 6 months, investigating the incidence rate 20 d after planting, and evaluating the disease resistance according to the 1-5 level standard. [Results] The two methods are similar to the natural transmission method. After inoculation, SCML occurred significantly, with high sensitivity and good reproducibility. The results of resistance identification were consistent with those of natural disease in the field. Through the two inoculation methods and field natural disease investigation, the resistance of 10 main cultivars to SCML was identified, which was true and reliable. [Conclusions] This study can provide standard varieties for identification of SCML resistance in the future.

Effectiveness of Rice Husk and Sugarcane Bagasse Ashes Blended Cement in Improving Properties of Concrete

This paper emphasized the use of rice husk ash (RHA) and sugarcane bagasse ash (SBA) in improving concrete properties, and also their combined effects on workability, compressive strength, flexural strength, permeability and water absorption capacity. Thus, in this study, the water-to-cement ratio was kept constant (0.45), the binder materials content for conventional mix was kept constant at (350 kg/m3) and the partial replacement of cement with RHASBA used was 5%, 10%, 15%, 20%, 25%, and 30% by weight of cement. The maximum compressive strength was noted at a 5% replacement level of cement with RHASBA. The Results showed that the optimum replacement of cement with RHASBA in concrete was 5%, which was found to increase the compressive strength by 15%, flexural strength by 3.4%, lowered permeability by 50%, lowered sorptivity by 11.34% as compared with control concrete at 90 days of curing time. The micro-structural test results further established that RHA and SBA have a high content of SiO2 which enables them to be more reactive in concrete and also revealed that the presence of RHASBA depletes Ca(OH)2 crystals, converting it into CaH2O4Si (C-S-H gel) leading to the strengthening of bond within the concrete matrix.

Climate Impact on the Productivity of Sugarcane Varieties in Ferke1 Industrial Plantations

This study assesses the climate impact on the productivity of five sugarcane varieties (R579, SP711406, M2593/92, M1400/86, and SP701006) in the industrial plantations of Ferké 1 sugar complex. It is a contribution to research efforts aimed at increasing the productivity of sugarcane varieties in the sugar fields. Also to support agricultural development and guarantee the income of planters. The sugarcane production data are from 2013 to 2017. Climatological data are measured and calculated continuously daily at the production site. In addition, the CMIP-5 (Coupled Model Intercomparison Project) climate database at 1º × 1º horizontal resolution was used for the predictability of crop yields of the 5 sugarcane varieties in the near future (2021-2050) and far future (2056-2075) to improve the quality of climate services to producers. The statistical methodological approach by multiple linear regression associated with the significativity test shows important and significant coefficients of determination (R2 > 0.90) between the yields of sugarcane varieties with certain climatic parameters such as minimum and maximum temperatures, insolation, global solar radiation, and potential evapotranspiration. The impact of rainfall has not been directly evaluated because the linear models do not explicitly show sensitivities to this parameter and the total water requirements for the plot are completely assured by irrigation. The future climate projections analyzed only from extreme thermal parameters (Tmax and Tmin) highlight their strong sensitivities with yields from an idealized model. In this model, we have assumed that the water supply needed by sugarcane is always met by irrigation on different plots. Moreover, linear models do not evolve fast enough in time and changes due to external environmental constraints are not too important at the plot scale. The projected thermic parameters can thus constitute a limiting factor for the producibility of sugarcane varieties either by excess or by default. In addition, the linear models used allowed us to observe the behavior of yields with respect to observed past climatic conditions. However, for future yields, there is no way to know if these regressions have the ability to predict them since they are based on projected weather conditions (i.e. CMIP5 data) marked by uncertainties. Additionally, none of the regression equations have been tested against independent observations.

Research Progress in Formation and Detection Method of Dextran in Sugarcane Production

Dextran in sugarcane production process is formed by Leuconostoc rnesenteroides. The content levels of dextran is related to sugarcane varieties, field condition （planting pattern, temperature, humidity, sunlight, soil, foreign material）, de- gree of injury （refractory cane, harvesting methods）, and can be rapidly and accu- rately measured by Dextran Immunonephelometric Test Kit. The presence of dextran indicates that sucrose has been lost, so sugarcane dextran is a direct and reliable indicator to measure sugarcane freshness and quality.

Effectiveness of Rice Husk and Sugarcane Bagasse Ashes Blended Cement in Improving Properties of Concrete

This paper emphasized the use of rice husk ash (RHA) and sugarcane bagasse ash (SBA) in improving concrete properties, and also their combined effects on workability, compressive strength, flexural strength, permeability and water absorption capacity. Thus, in this study, the water-to-cement ratio was kept constant (0.45), the binder materials content for conventional mix was kept constant at (350 kg/m3) and the partial replacement of cement with RHASBA used was 5%, 10%, 15%, 20%, 25%, and 30% by weight of cement. The maximum compressive strength was noted at a 5% replacement level of cement with RHASBA. The Results showed that the optimum replacement of cement with RHASBA in concrete was 5%, which was found to increase the compressive strength by 15%, flexural strength by 3.4%, lowered permeability by 50%, lowered sorptivity by 11.34% as compared with control concrete at 90 days of curing time. The micro-structural test results further established that RHA and SBA have a high content of SiO2 which enables them to be more reactive in concrete and also revealed that the presence of RHASBA depletes Ca(OH)2 crystals, converting it into CaH2O4Si (C-S-H gel) leading to the strengthening of bond within the concrete matrix.

Influence of Climate on Sugarcane Yield in Côte d’Ivoire: Case of the Ferkessédougou Region

This study aims to understand the current climatic trends and explain the possible losses of agricultural yields. To achieve this objective, this work characterized the evolution of extreme temperature indices in the sugar complexes of Ferké 1 and Ferké 2, two stations located in the northern part of C?te d'Ivoire. The onset and cessation dates of the rainy season and the length of the rainy season were investigated. The agricultural and climatic data were obtained from each sugar complex. The period of study ranges from 2002 to 2019 in Ferké 1 and Ferké 2. The results show significant upward trends in extreme temperature indices. The analysis of sugarcane yield associated with the different climatic parameters shows no significant results in general. However, on the Ferkessédougou sugar complexes, the results highlight that maximum and minimum temperatures could be the variables that influence most yield production. The maximum temperature with coefficients of 1.60 and 0.77 at Ferké 1 and Ferké 2 respectively seems to contribute to an increase in yield while the minimum temperature with coefficients of -0.98 and -0.22 at Ferké 1 and Ferké 2 respectively could lead to a loss in yield. The results obtained with the Single Linear Regression (SLR) and the Multiple Linear Regression (MLR) models also highlight the strong influence of minimum and maximum temperatures.

Sugarcane Requirement of Water and Effect of Fertigation on Sugarcane

The sugarcane is a main sugar crop in China. The seasonal drying is one of the problems that influence the improvement of output and quality of sugarcane in China. The experimental results for the water requirement and fertigation of the sugarcane showed, that the distribution of the rainfall in spring, autumn and winter, all could not suit the requirements for sugarcane growth. Detailedly, it can suit that sugarcane requirement of 74.4%, 68.6% and 35.7% respectively at seedling emerging stage, tillering stage and mature stage according to sugarcane growth. The drought has already limited the yield of sugarcane. Application of fertigation can enhance the fertilizer use efficiency, and it is also a water saving technique in sugarcane production.

Occurrence Dynamics and Control Strategies of Major Pests and Diseases of Sugarcane in Yunnan

In recent years, the occurrence and harm of many kinds of pests and diseases have become increasingly serious in Yunnan sugarcane areas, which have greatly affected the sustained and stable development of sugarcane production, so the situation of their prevention and control has become rigorous. In order to scien- tifically and effectively prevent and control pests and diseases of sugarcane to en- sure the safety of sugarcane production, on the basis of an investigation and re- search, according to the present situation of sugarcane production in Yunnan, the occurrence dynamics of major pests and diseases of sugarcane were analyzed, and corresponding control strategies were proposed according to the major pests and diseases as well as their occurrence and damage characteristics.

Effects of Different Fertilization Treatments on Soil Microbial Biomass,Soil Enzyme Activities and Related Nutrients in Continuous-cropping Sugarcane Field

[Objective] This study was conducted to expound the fertility improvement effect in continuous-cropping sugarcane field and provide reference for establishment of rational sugarcane fertilization system and improvement of soil quality in continuous-cropping sugarcane field. [Method] The soil in the experimental region is latosolic red soil which was planted with sugarcane for 11 years continuously, and 8 treatments including sole application of chemical fertilizers, sole application of organ- ic fertilizer, and combined application of organic fertilizer and chemical fertilizers were designed according to different fertilization measures. The effects of different fertilization treatments on soil microbial biomass, soil enzyme activities and related fertility factors were determined. [Result} Different fertilization treatments all showed soil microbial biomass N, C and P and activities of soil acid phosphatase, catalase, sucrase and urease higher than the CK. Soil microbial biomass N increased by 5.56%-67.13%, soil microbial biomass C increased by 4.01%-20.40%, and soil mi- crobial biomass P increased by 6.39%-67.02%. The activity of acid phosphatase was improved by 12.96%-35.19%, the activity of catalase was improved by 18.24% -78.93%, the activity of sucrase was improved by 3.00%-42.00%, and the activity of urease was improved by 1.21%-23.43%. However, the soil nutrients of different fertilization treatments increased non-significantly （P〉0.05）. Soil microbial biomass N, C and P and activities of acid phosphatase, catalase and urease were in significant （P〈0.05） or very significant correlation （P〈0.01） with contents of soil rapidly available P, rapidly available K and total N. [Conclusion] The evaluation of improvement of soil fertility in continuous-cropping sugarcane field using soil microbial biomass and enzyme activities as indexes is more comprehensive and sensitive.

Characteristics and Evaluation of Soil Fertility in Sugarcane-producing Areas in Guangxi

[Objective] Soil fertility in Guangxi sugarcane-growing areas was evaluated to provide theoretical reference for sugarcane scientific planting and fertilization and further optimize evaluation system for soil fertility in Guangxi sugarcane-growing ar- eas. [Method] Nutrients of soil samples in 3 006 plots were measured, and fertility characters were evaluated based on the improved Nemerow composite index method. [Result] Soil in Guangxi sugarcane-growing area was sticky and acidic with an average pH value of 4.94. Available B was insufficient with an average content of 0.24 mg/kg. Exchangeable Mg was low with an average content of 62.34 mg/kg. Organic matter content was moderate. The contents of alkali N, rapidly available P, rapidly available K, available Cu, available Zn, available Mn and available Ca were moderate. However, unlike the soil in high-yield areas, there was unbalanced nutri- tion distribution. According to Nemerow integrated index method, soil fertility in sug- arcane-growing areas was grade lU, namely low fertility. The integrated fertility index was 0.56. Individual fertility indexes including rapid available Cu, rapidly available Zn, exchangeable Mg, available Mn, rapidly available manganese Mn and alkali N were at poor level. Other indexes were at medium or high level. [Conclusion] It could be concluded that soil fertility in Guangxi sugarcane-growing area was gener- ally low, The above-mentioned evaluation method could help to build a quantified and scientific soil fertility evaluation system and reduce the impacts of subjective factors during evaluation process.

Effects of Different Plastic films Mulching on Soil Temperature and Moisture, the Growth and Yield of Sugarcane

[Objective] The effects of different plastic films mulching on soil temperature and moisture, and growth and yield of sugarcane were discussed in order to provide references for using different plastic film in sugarcane pro-duction. [Method]Four kinds of plastic films viz., normal colorless transparent plastic film, milky photodegradation weeding plastic film, black plastic film and gray-black plastic film were used in sugarcane cultivation by using no film mulching as the control. Soil temperature and moisture were measured during plastic film mulching period, and sugarcane agronomic traits such as emergence rate, tillering rate, plant hight, stalk diameter and effective stalk number were investigated during growth period, the cane yield and economic benefits were calculated during harvest period. [Result] The results showed that plastic film mulching could significantly increase soil temperature and moisture. Com-pared with the control, soil temperature was increased by 0.3-0.8 ℃ in three plastic films mulching treatment except for gray-black plastic film mulching. The soil moisture of all mulching treatments was 10.1%-17.4% higher than the control. Furthermore, the seedling emergence rate, tillering rate, effective stalk number and cane yield also could be improved using plastic film mulching,which were increased by 0.8%-9.9%, 20.6%-34.9%, 5190-10980 stalks/hm^2and6.4%-14.9% as compared to the control,while plant height and stalk diameter were found to be no significant effect by plastic film mulching. The results of benefit analysis indicated that, milky photodegradation weeding film mulching had the highest economic benefit, the second were normal colorless transparent plastic film mulching and black plastic film mulching, which were 5 987.2, 1 876.5 and 1 813.5 Yuan/hm^2 higher than the control. The gray-black film mulching treatment had poor benefit.[Conclusion] The milky photodegradation weeding plastic film could be vigorously extended in sugarcane production. Normal colorless transparent plastic film and black plastic film could be ex-tended gradually as a new kind of plastic film. The grayblack film should not be used for its higher cost and more thickness.

ISSR Polymorphism Analysis on F_2 Hybrids between Sugarcane and Erianthus fulvus

[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.