Sweet protein thanmatin is a noncaloric sweetener that has many advantages such as high sweetness intensity,low calories,no toxicity and nice sweet taste.The review is given about the structure,quality and possible sw...Sweet protein thanmatin is a noncaloric sweetener that has many advantages such as high sweetness intensity,low calories,no toxicity and nice sweet taste.The review is given about the structure,quality and possible sweet mechanism of sweet protein thaumatin.The development and application of sweet protein thaumatin will have broad prospect in the aspects of foodstuff industry and plant reformation.展开更多
Genes encoding thaumatin-like protein(TLPs) are frequently found in fungal genomes.However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbella...Genes encoding thaumatin-like protein(TLPs) are frequently found in fungal genomes.However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbellatus. The full-length coding sequence of PuTLP1, PuTLP2 and PuTLP3 were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that P. umbellatus PuTLP1, PuTLP2 and PuTLP3 were clustered with sequences from Gloeophyllum trabeum, Trametes versicolor and Stereum hirsutum, respectively. The expression patterns of the three TLP genes were higher in P. umbellatus with Armillaria mellea infection than in the sclerotia without A. mellea. Furthermore, over-expression of three PuTLPs were carried out in Escherichia coli BL21(DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that PuTLP1, PuTLP2 and PuTLP3 in P. umbellatus may be involved in the defense response to A. mellea infections.展开更多
文摘Sweet protein thanmatin is a noncaloric sweetener that has many advantages such as high sweetness intensity,low calories,no toxicity and nice sweet taste.The review is given about the structure,quality and possible sweet mechanism of sweet protein thaumatin.The development and application of sweet protein thaumatin will have broad prospect in the aspects of foodstuff industry and plant reformation.
基金supported by grants of Coal Base Key Scientific and Technology Project of Shanxi Province "Research and Demonstration of High Efficient Carbon Cycle in Edible Fungi" (No.FT2014-03)Evaluation of Germplasm Resources of Chinese Medicinal Herbs in Hebei Mountain Area and Demonstration of Imitation Wild Cultivation Technology(No.16232503D-X-2)Special Program for Applied Research on Super Computation of the NSFC-Guangdong Joint Fund(the second phase)
文摘Genes encoding thaumatin-like protein(TLPs) are frequently found in fungal genomes.However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbellatus. The full-length coding sequence of PuTLP1, PuTLP2 and PuTLP3 were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that P. umbellatus PuTLP1, PuTLP2 and PuTLP3 were clustered with sequences from Gloeophyllum trabeum, Trametes versicolor and Stereum hirsutum, respectively. The expression patterns of the three TLP genes were higher in P. umbellatus with Armillaria mellea infection than in the sclerotia without A. mellea. Furthermore, over-expression of three PuTLPs were carried out in Escherichia coli BL21(DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that PuTLP1, PuTLP2 and PuTLP3 in P. umbellatus may be involved in the defense response to A. mellea infections.
