Thoughts and Practice on Some Problems about Research and Application of Two-Line Hybrid Rice

The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.

Research on Theories and Techniques of Irrigation for Safeguarding Seed Production of Two-Line Hybrid Rice

By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei＇ai64S, were measured. Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1 ℃. For the present study, the following irrigation techniques were put forth： the water depth of 15-20 cm, current water used, irrigating after 17：00 and bailing at 10：00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice.

Causes of Poor Grain Plumpness of Two-Line Hybrids and Their Relationships to the Contents of Hormones in the Rice Grain

This study was designed to elucidate the grain filling characteristics and the causes of poor grain plumpness of some two-line hybrid rice combinations and their hormonal mechanism. Six varieties, including three two-line hybrid rice combinations, that show differences in seed-setting and grain filling, were used. And the contents of starch, sucrose, zeatin （Z） ＋ zeatin riboside （ZR）, indole-3-acetic acid （IAA）, and abscisic acid （ABA）, the ethylene evolution rate, activities of sucrose synthase （SuSase） and starch synthase （StSase） in grains, the seed-setting and grain filling rate were investigated. The correlations amongst these were analyzed. The results showed that the poor grain filling of two-line hybrids was mainly attributed to the higher unfilled grain rate and the lower filling degree of inferior grains. During the early and mid grain filling periods, the sucrose content in inferior grains was greater than that in superior grains for the combinations with poor grain filling, indicating that the substrate concentration was not the principal factor for their slow grain filling and poor grain plumpness of the inferior grains of two-line hybrids. Z ＋ ZR, IAA, and ABA in superior grains were obviously greater than those in inferior grains at early grain filling stage. The maximum and mean contents of Z ＋ ZR, IAA, and ABA were positively very significantly correlated with the maximum and mean grain-filling rate, filling degree, and grain weight. The evolution rate of ethylene was greater in inferior grains than in superior grains and greater for the combinations with poor grain plumpness than those with good grain plumpness at the early or mid filling stages. The evolution rate of ethylene was negatively and significantly correlated with the grain filling rate, the grain filling degree, and the grain weight. Spraying ethephon （ethylene-releasing agent） at the early grain filling stage increased the evolution rate of ethylene, reduced the ABA content and activities of SuSase and StSase, and decreased the grain filling degree and the grain weight. The results were reversed when cobatous nitrate （an inhibitor of ethylene synthesis） was applied. The results suggested that the hormones and their balance play a role in the regulation of grain filling and enzymatic activities, and the poor grain filling is attributed to the low contents of Z ＋ ZR, IAA, and ABA, and the high evolution rate of ethylene in the inferior grains of some two-line hybrid rice combinations. The results suggested that hormones play important roles in the grain filling of some two-line hybrid rice combinations, and their filling degree can be improved by regulating the hormonal contents.

Semi-Markovian Model of Two-Line Queuing System with Losses

In the present paper, to build model of two-line queuing system with losses GI/G/2/0, the approach introduced by V.S. Korolyuk and A.F. Turbin, is used. It is based on application of the theory of semi-Markov processes with arbitrary phase space of states. This approach allows us to omit some restrictions. The stationary characteristics of the system have been defined, assuming that the incoming flow of requests and their service times have distributions of general form. The particular cases of the system were considered. The used approach can be useful for modeling systems of various purposes.

Breeding of a Water-saving Drought-resistant Two-line Hybrid Rice Variety Wanliangyou 1008

Drought is one of the important factors limiting rice production,and the application of new water-saving rice varieties is urgently needed in rice production.Wanliangyou 1008 bred by Institute of Rice Research,Anhui Academy of Agricultural Sciences is a new two line hybrid rice variety with some good traits,including high drought resistance,high yield and good disease resistance.Wanlingyou 1008 was obtained by crossing drought-resistant high-quality restorer line DJ1008 and disease-resistant two-line sterile line Wan 25S.In this paper,the breeding process,characteristics and seed production technology of the variety were described in detail.

Exploiting Crop Heterosis by Two-line System Hybrids : Current Status and Future Prospects

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Calibration of atmospheric density model using two-line element data

For satellites in orbits,most perturbations can be well modeled;however the inaccuracy of the atmospheric density model remains the biggest error source in orbit determination and prediction.The commonly used empirical atmospheric density models,such as Jacchia,NRLMSISE,DTM,and Russian GOST,still have a relative error of about 10%-30%.Because of the uncertainty in the atmospheric density distribution,high accuracy estimation of the atmospheric density cannot be achieved using a deterministic model.A better way to improve the accuracy is to calibrate the model with updated measurements.Twoline element(TLE)sets provide accessible orbital data,which can be used in the model calibration.In this paper,an algorithm for calibrating the atmospheric density model is developed.First,the density distribution of the atmosphere is represented by a power series expansion whose coefficients are denoted by the spherical harmonic expansions.Then,the useful historical TLE data are selected.The ballistic coefficients of the objects are estimated using the BSTAR data in TLEs,and the parameterized model is calibrated by solving a nonlinear least squares problem.Simulation results show that the prediction error is reduced using the proposed calibration algorithm.

“一点两线三面”创新实验平台的构建与实践

传统化学实验室功能单一、分布零散、共享性差,导致实验用房和实验设备利用效率低,这种建设模式不利于多学科知识的交叉融合,一定程度上影响学生综合素质的发展。本研究秉承“建优质平台,抓实践育人,树创新理念,育创新人才”的指导思想,在化学实验室资源整合共享、利用率提升、开放式管理以及创新应用型人才培养质量提升等方面积极进行教学改革,经过多年探索,搭建起“以提升学生创新实践能力为着力点”“教学科研两条主线”“教学科研三层面”的创新实验(训)平台,取得了一定成效,将为同类高校的创新实验(训)平台建设提供参考。

光温敏两系不育系‘稆S’的选育与应用

[目的]选育优良的光温敏两系不育系,以提高其杂种后代的产量和品质。[方法]采用光温敏两系不育系‘RGD-7S’为母本与三系保持系‘中浙B’杂交,经5年9代的系谱法选育,育成不育性稳定、稻米品质优、异交特性好、株叶形态优良的光温敏两系不育系‘稆S’,并分析了其主要特征特性及杂种优势。[结果]‘稆S’不育性稳定,育性转换临界温度<23℃,稳定不育期127 d左右。该不育系制种产量高,异交特性好,柱头外露率78.62%,其中双边外露率39.74%。采用‘稆S’配组的高产、优质两系杂交稻组合‘稆两优质美’先后通过了福建省和海南省农作物品种审定。[结论]光温敏两系不育系‘稆S’不育性稳定,育性转换临界温度低,稻米品质优,配合力强,应用前景广泛。

Breeding and Application Techniques of a New Rice Variety Liliang-you 3822 with High Yield and Disease Resistance

Liliangyou 3822 is a novel indica hybrid rice variety that exhibits disease resistance,high yield,lodging resistance,and late maturity.It employs a self-selected two-line sterile line,Li 38S,and a self-selected restorer line,R22.This variety was subjected to a regional test of indica late-maturing groups in the middle and lower reaches of the Yangtze River in 2020.The results demonstrated that the average yield of the variety was 9.95 t/hm 2,which was 10.67%higher than that of the control Fengliangyou 4,indicating a highly significant yield increase.In the continuous test in 2021,the average yield was 9.74 t/hm 2,representing a 6.52%increase over the control,which also exhibited a significant increase.Finally,the average yield of the two years regional test was 9.84 t/hm 2,which was 8.58%higher than that of the control.In the 2021 production test,the average yield of the variety was 9.32 t/hm 2,which was 12.19%higher than that of the control,indicating a remarkably significant yield increase.In 2022,the variety was validated by the National Crop Variety Approval Committee(GSD 20220143).

非圆形隧洞衬砌支护下应力与位移的新解法

以往在利用平面弹性复变函数方法进行衬砌支护作用下非圆形隧洞的力学分析时,一般将洞室外域保角映射到象平面的单位圆外域,并认为可以利用同一个映射函数将衬砌截面映射到象平面的圆环域内。当衬砌厚度很薄时,这种方法是可行的,而当衬砌相对较厚时,利用同一映射函数所得到的衬砌截面形状发生严重失真。为解决此问题,本文引入两套映射函数,分别将洞室外域映射到象平面的单位圆的圆外域,衬砌截面映射到圆环域。这种方法可以适应于不同的衬砌厚度,利用这种方式获得的衬砌截面,在衬砌较厚时仍能保证衬砌厚度相对均匀。在求解解析函数过程中不再采用传统的幂级数解法,而是使用边界配点法,将两套映射函数结合,获得了该问题的应力解和位移解。本文以马蹄形隧洞为例,求解结果与ANSYS软件所得数值解吻合很好,分析了衬砌厚度对隧洞应力和位移的影响。根据本文解答可以方便、快捷地进行相近条件下隧道的初步设计。

海南两系杂交稻制种适宜区域与时段选择

掌握两系杂交稻在海南制种的气象灾害风险,是科学合理安排两系杂交稻制种生长期的重要依据。针对两系杂交水稻制种过程中的3个关键阶段(育性敏感安全期、抽穗扬花授粉安全期和灌浆安全期)的气象灾害概率进行分析,通过数据分析和实例验证得出各地区制种的安全时段,以及需重点考虑的气象灾害。海南两系杂交水稻育性敏感安全期可安排在3月上旬至4月中旬,三亚、陵水地区从3月上旬开始;乐东、东方、昌江从3月下旬至4月上旬开始;临高在4月中旬至下旬左右,不同界限温度变化略有先后。各地花期的阴雨天气较为随机且概率较小;临高、昌江地区制种重点关注灌浆安全期干热风等危害,提早播种。目前海南水稻制种区域和时段合理,可根据不育系材料不育起点温度阈值和耐受低温时间长短,适当调整播种时段。

免疫治疗药物主体间性“双线”教学探索

免疫治疗药物是一类通过调节人体免疫系统功能治疗疾病的药物,作为临床一线用药,其在缓解疾病症状,改善重度炎症带来的不良预后方面尤为重要。近年来,伴随互联网技术迅速发展,科技产业不断掀起创新浪潮,国家出台多项指导意见,要求学校加强线上教学,推进“互联网+教育”发展。线上教学不受时间、区域的影响,促进了资源共享,激发了学生兴趣。然而,经过实践性检验,单一线上教学或线下教学模式均存在诸多弊端。本文以重度炎症免疫用药为例,融合主体间性的教学理念,进行免疫治疗药物的线上线下“双线”教学探索。以学生作为学习的主体,强调个体学习的重要性,解决了单一教学模式的弊端。主体间性“双线”教学模式的探索与应用,对互联网时代下的教育改革具有重要指导意义。

福建两系杂交稻制种安全高产的精细气候适宜性区划

利用福建省67个国家气象观测站1971-2020年气象资料以及地理信息资料、两系杂交稻制种基地调查资料,构建了两系杂交稻制种育性转换敏感期气候风险模型与风险等级指标、扬花授粉期天气综合危害指数模型与等级指标两个安全期气候区划指标,选择不育系育性转换起点温度23℃开展研究,基于两系杂交稻制种气候适宜性分区等级标准,采用多层复合方法,开展基于GIS的福建省两系杂交稻制种安全高产精细气候适宜性区划。结果表明:育性敏感期安排在7月上旬-下旬、7月中旬-8月上旬,扬花授粉期安排在8月上旬-中旬,适宜区分布在福建北部、西南部等县市海拔300m以下的区域,次适宜区分布在福建北部、西南部等县市海拔300-500m的区域;育性敏感期安排在7月下旬-8月中旬、8月上旬-下旬,扬花授粉期8月下旬-9月上旬,适宜区分布在福建北部、西南部等县市海拔400m以下的区域,次适宜区分布在福建北部、西南部等县市海拔400-500m的区域;育性敏感期安排在7月上旬-8月下旬,扬花授粉期安排在8月上旬-9月上旬,低适宜区分布在海拔500-1000m的区域,不适宜区分布在海拔1000m以上区域。

两系杂交水稻新组合两优5078高产制种技术

两优5078是合肥丰乐种业股份有限公司与安徽省农业科学院水稻研究所共同选育的籼型两系杂交水稻新品种,具有株型好、产量高、适口性好等优点,适合在长江中下游作一季中稻种植,2019年通过国家审定。该文简述了该品种的亲本来源、特征特性及制种技术要点。

两系杂交水稻新组合徽两优9192高产制种技术

徽两优9192是合肥丰乐种业股份有限公司与安徽省农业科学院水稻研究所共同选育的籼型两系杂交水稻新品种,2018年通过国家审定,具有丰产性良好、株型好、生育期适中、抗性好等特点,适宜在湖北省(武陵山区除外)、湖南省(武陵山区除外)、江西省、安徽省、江苏省的长江流域稻区以及浙江省中稻区、福建省北部稻区、河南省南部稻区的稻瘟病轻发区作一季中稻种植。根据徽两优9192的亲本特性及制种地区福建省建宁县亚热带季风气候特点,从选择制种区域、田间管理等方面介绍徽两优9192的制种技术,以期为该品种大面积推广种植提供参考依据。

优质抗病水稻品种宛两优2165的选育与应用

宛两优2165系福建省农业科学院水稻研究所、福建金品农业科技股份有限公司用自育的两系不育系宛S与恢复系福恢2165配组育成的两系杂交稻新品种,于2022年通过福建省品种审定。表现熟期适中、米质优、中抗稻瘟病、易于制种等特点。介绍了宛两优2165的选育过程、特征特性、栽培和制种技术。

矩形管下气相螺旋爆轰的结构及传播方式

为探索极限条件下矩形管道截面长宽比对于螺旋爆轰传播的影响,采用基于五阶WENO有限差分格式和两步总包反应模型的Euler方程,对三维气相螺旋爆轰波在矩形截面管道中的结构及其传播方式进行了数值研究。通过模拟不同管道截面尺寸下爆轰波的三波线运动轨迹、流场分布及高压印记结构,揭示了截面几何尺寸对气相临界爆轰波稳定传播的影响规律。结果表明:螺旋爆轰能在一定范围的小管道截面尺寸内通过横、竖两条三波线及其相互作用形成的斜三波线的运动来维持传播;随着管道截面尺寸长宽比的增加,螺旋爆轰在壁面上形成的高压印记逐渐由倾斜的条带结构变成局部点状分布结构,波阵面上的斜三波线的轨迹也由方管中沿着单一方向的圆周运动逐渐发展为具有转向机制的复杂运动轨迹;当长宽比进一步增加时,三维螺旋爆轰存在向二维结构的单头爆轰结构退化的趋势。

基于两点一线为解剖标志的胃底体一体化游离方式在腹腔镜胃袖状切除术中的临床应用

目的 探讨基于膜解剖指导下的胃左系膜、胃后系膜、胰腺系膜三三交汇处TJ点至左侧膈肌脚内侧缘顶点“两点一线”为解剖标志的胃底体一体化游离方式(two points and one line model, TPOL模型)腹腔镜袖状胃切除术(LSG)的可行性与安全性。方法 我院2019年1月~2022年1月收治的肥胖病或肥胖合并代谢综合征病人135例,均行LSG治疗。根据胃底体游离方式不同,分为两组,研究组68例,采用TPOL模型LSG手术。对照组67例,采用常规LSG手术。比较两组病人手术时间、胃底游离时间、术中出血量、住院时间、术后胃漏、出血、胃食管反流发生率等指标。结果 所有病人均顺利完成LSG手术,术后随访12~36个月。研究组与对照组手术时间分别为(56.13±10.56)分钟和(62.45±12.74)分钟,胃底游离时间分别为(6.34±4.16)分钟和(12.58±6.37)分钟,住院时间分别为(3.84±0.42)天和(4.06±0.69)天,术中出血量分别为(10.87±1.28)ml和(15.56±3.39)ml,术后胃食管反流发生率分别为5例(7.35%)和13例(19.40%),两组比较差异有统计学意义(P<0.05)。两组术后12个月多余体重减少以及术后出血、胃漏等并发症比较,差异无统计学意义(P>0.05)。结论 基于膜解剖指导下的胃后TJ点至左侧膈肌脚内侧缘顶点“两点一线”为解剖标志的胃底体一体化游离方式(TPOL模型)LSG术可高效、安全、完整游离胃底,对LSG的胃底规范化游离具有临床价值。

两系杂交水稻组合Q两优851制种技术

Q两优851是利用自育不育系全151S和自育优质恢复系YR851配组育成的两系杂交籼稻组合,2019和2020年分别通过长江中下游和长江上游审定。本文分析了该组合亲本的特征特性、不育系的选育过程,基于近几年栽培制种实践,总结了该组合高产安全制种技术,为水稻制种工作提供参考。