In recent years,the use of coronary functional evaluation derived by fractional flow reserve(FFR)to guide percutaneous coronary intervention(PCI)treatment has been recommended by several mainstream guidelines.Typicall...In recent years,the use of coronary functional evaluation derived by fractional flow reserve(FFR)to guide percutaneous coronary intervention(PCI)treatment has been recommended by several mainstream guidelines.Typically,FFR>0.80 in coronary artery indicates the lesions do not affect the coronary blood flow.展开更多
The therapeutic effects of activating blood circulation to remove stasis of Chinese medicine (CM) in the prevention and treatment of atherosclerotic diseases such as coronary artery disease and angina are generally ...The therapeutic effects of activating blood circulation to remove stasis of Chinese medicine (CM) in the prevention and treatment of atherosclerotic diseases such as coronary artery disease and angina are generally acknowledged. In recent years, to further improve the therapeutic effects of those Chinese herbs on such diseases, we have carried out a series of studies of CM medications with the function of clearing heat and removing toxic substances combined with activating blood circulation to remove stasis.展开更多
Very small superparamagnetic iron oxide nanoparticles (VSOPs) rapidly accumulate in atherosclerotic lesions, thereby enabling plaque visualization by magnetic resonance imaging (MRI). This study was performed to i...Very small superparamagnetic iron oxide nanoparticles (VSOPs) rapidly accumulate in atherosclerotic lesions, thereby enabling plaque visualization by magnetic resonance imaging (MRI). This study was performed to identify the uptake mechanisms of VSOPs into atherosclerotic plaques. Low-density lipoprotein receptor-deficient (LDLR^-/-) mice with advanced atherosclerosis were analyzed using MRI and transmission electron microscopy (TEM) at various time points after intravenous administration of VSOPs. Post-mortem MRI detected VSOP labeling of atherosclerotic plaques 10 min after injection, and the signal increased over the first 3 h. TEM revealed that the intensive plaque labeling was mediated by accelerated transcytosis of VSOPs through endothelial cells overlaying atherosclerotic lesions. Experiments with endocytosis inhibitors and small interfering RNA (siRNA) revealed a dynamin-dependent mechanism involving both clathrin- and caveolin-mediated processes. In cell culture experiments, endothelial VSOP uptake was enhanced under proatherogenic flow and TNFα stimulation, conditions that are both present in plaque areas. Our study demonstrates that VSOPs enable non-invasive MRI assessment of accelerated endothelial transcytosis, an important pathomechanism in atherosclerotic plaque formation.展开更多
Objective: To observe the effects of red yeast rice(RYR) on blood lipid levels, aortic atherosclerosis(AS), and plaque stability in apolipoprotein E gene knockout(Apo E-/-) mice. Methods: Twentyfour Apo E-/-mi...Objective: To observe the effects of red yeast rice(RYR) on blood lipid levels, aortic atherosclerosis(AS), and plaque stability in apolipoprotein E gene knockout(Apo E-/-) mice. Methods: Twentyfour Apo E-/-mice were fed with a high-fat diet starting from 6 weeks of age. Mice were randomized into three groups(n = 8 in each group): model group(Apo E-/-group), RYR group(Apo E-/-+ RYR group), and simvastatin group(Apo E-/-+ simvastatin group). Eight 6-week-old C57BL/6 mice were assigned as the control group and fed with a basic diet. After 36 weeks, plasma lipids and inflammatory factors were measured. Aortic atherosclerotic lesions by microscope, scanning electron microscope and transmission electron microscope were observed. Plasma levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were measured with enzymelinked immunosorbent assay. The level of high sensitivity C-reaction protein(Hs-CRP) was detected by the scattering immunoturbidimetric assay. Protein expression of matrix metalloproteinase-9(MMP-9) and nuclear factor κB(NF-κB) in aorta were tested by immunohistochemistry. Results: Compared with the model group, treatment with RYR significantly decreased the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, lipoprotein(a), and apolipoprotein B100 in Apo E-/-mice(P〈0.01). Compared with the model group, treatment with RYR decreased the levels of Hs-CRP, IL-6, and TNF-α(P〈0.01). RYR also reduced the protein levels of NF-κB and MMP-9 of the aorta. Conclusions: RYR has the anti-atherosclerotic and stabilizing unstable plaque effects. The mechanism might be related to the inflammatory signaling pathways.展开更多
文摘In recent years,the use of coronary functional evaluation derived by fractional flow reserve(FFR)to guide percutaneous coronary intervention(PCI)treatment has been recommended by several mainstream guidelines.Typically,FFR>0.80 in coronary artery indicates the lesions do not affect the coronary blood flow.
文摘The therapeutic effects of activating blood circulation to remove stasis of Chinese medicine (CM) in the prevention and treatment of atherosclerotic diseases such as coronary artery disease and angina are generally acknowledged. In recent years, to further improve the therapeutic effects of those Chinese herbs on such diseases, we have carried out a series of studies of CM medications with the function of clearing heat and removing toxic substances combined with activating blood circulation to remove stasis.
文摘Very small superparamagnetic iron oxide nanoparticles (VSOPs) rapidly accumulate in atherosclerotic lesions, thereby enabling plaque visualization by magnetic resonance imaging (MRI). This study was performed to identify the uptake mechanisms of VSOPs into atherosclerotic plaques. Low-density lipoprotein receptor-deficient (LDLR^-/-) mice with advanced atherosclerosis were analyzed using MRI and transmission electron microscopy (TEM) at various time points after intravenous administration of VSOPs. Post-mortem MRI detected VSOP labeling of atherosclerotic plaques 10 min after injection, and the signal increased over the first 3 h. TEM revealed that the intensive plaque labeling was mediated by accelerated transcytosis of VSOPs through endothelial cells overlaying atherosclerotic lesions. Experiments with endocytosis inhibitors and small interfering RNA (siRNA) revealed a dynamin-dependent mechanism involving both clathrin- and caveolin-mediated processes. In cell culture experiments, endothelial VSOP uptake was enhanced under proatherogenic flow and TNFα stimulation, conditions that are both present in plaque areas. Our study demonstrates that VSOPs enable non-invasive MRI assessment of accelerated endothelial transcytosis, an important pathomechanism in atherosclerotic plaque formation.
基金Supported by the National Natural Science Foundation of China(No.81102721,No.81573821 and No.81202809)Beijing Natural Science Foundation(No.7172185)+3 种基金Technologic Item of State Administration of Traditional Chinese Medicine(No.04-05LP30,JDZX2015262)Chinese National Scientific Fund for Post-doctor(No.2015M570220)Its Special Aid Item(No.2016T90194)the Key Technologies R&D Program of Shandong Province,China(No.2010 GSF 10289)
文摘Objective: To observe the effects of red yeast rice(RYR) on blood lipid levels, aortic atherosclerosis(AS), and plaque stability in apolipoprotein E gene knockout(Apo E-/-) mice. Methods: Twentyfour Apo E-/-mice were fed with a high-fat diet starting from 6 weeks of age. Mice were randomized into three groups(n = 8 in each group): model group(Apo E-/-group), RYR group(Apo E-/-+ RYR group), and simvastatin group(Apo E-/-+ simvastatin group). Eight 6-week-old C57BL/6 mice were assigned as the control group and fed with a basic diet. After 36 weeks, plasma lipids and inflammatory factors were measured. Aortic atherosclerotic lesions by microscope, scanning electron microscope and transmission electron microscope were observed. Plasma levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were measured with enzymelinked immunosorbent assay. The level of high sensitivity C-reaction protein(Hs-CRP) was detected by the scattering immunoturbidimetric assay. Protein expression of matrix metalloproteinase-9(MMP-9) and nuclear factor κB(NF-κB) in aorta were tested by immunohistochemistry. Results: Compared with the model group, treatment with RYR significantly decreased the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, lipoprotein(a), and apolipoprotein B100 in Apo E-/-mice(P〈0.01). Compared with the model group, treatment with RYR decreased the levels of Hs-CRP, IL-6, and TNF-α(P〈0.01). RYR also reduced the protein levels of NF-κB and MMP-9 of the aorta. Conclusions: RYR has the anti-atherosclerotic and stabilizing unstable plaque effects. The mechanism might be related to the inflammatory signaling pathways.