Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.

Molecular cloning and ontogenesis expression of fatty acid transport protein-1 in yellow-feathered broilers

Fatty acid transport protein-1 （FATP-1） is one of the important transporter proteins involved in fatty acid transmembrane transport and fat deposition. To study the relationship between FATP-1 mRNA expression and fat deposition, chicken （Gallus gallus） FATP-1 sequence was first cloned by rapid amplification of cDNA ends （RACE）. Tissue samples of chest muscle, leg muscle, subcutaneous fat, and abdominal fat were collected from six male and six female broilers each, at 22 days, 29 days, and 42 days, respectively. The tissue specificity and ontogenesis expression pattern of the FATP-1 mRNA of yellow-feathered broilers was studied by real-time reverse transcription polymerase chain reaction （RT-PCR）, and the fat deposition laws in different tissues were also compared. A 2,488 bp cDNA sequence of chicken FATP-1 was cloned by RACE （GenBank accession no. DQ352834）, including 547 bp 3＇ end untranslated region （URT） and 1,941 bp open reading frame （ORF）. Chicken FATP-1 encoded 646 amino acid residues, which shared 83.9% and 83.0% identity with those of human and rat, respectively. The results of quantitative PCR demonstrated a constant FATP-1 mRNA expression level in the chest muscle and subcutaneous fat of both male and female broilers at three stages, whereas the expression level of the FATP-1 mRNA in the leg muscle at 42 days was significantly higher than that at 22 days or 29 days. In the abdominal fat of male broilers, the gene expression significantly increased with age, whereas the female broilers showed a dramatic downregulation of FATP-1 expression in abdominal fat at 42 days. This suggested a typical tissue-and gender-specific expression pattern of chicken FATP-1, mediating the specific process of fatty acid transport or utilization in muscle and adipose tissues.

Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers

L-theanine, a natural nonprotein amino acid with a high biological activity, is reported to exert anti-stress properties. An experiment with a 3 ? 2 factorial arrangement was conducted to investigate the effects of dietary L-theanine on growth performance and immune function in lipopolysaccharide(LPS)-challenged broilers. A total of 432 one-day-old male yellow-feathered broilers were randomly assigned to 3 dietary treatments(control, antibiotic and L-theanine diets) with 2 subgroups of each(6 replicate cages;12 birds/cage). Birds from each subgroup of the 3 dietary treatments were intra-abdominally injected with the same amount of LPS or saline at 24, 25, 26 d of age. Both dietary L-theanine and antibiotic improved(P < 0.05) the growth performance of birds before LPS injection(d 1 to 21). The effect of dietary L-theanine was better(P < 0.05) than that of antibiotic. Lipopolysaccharide decreased feed intake(FI) and body weight gain(BWG) from d 22 to 28(P < 0.05), BWG and feed to gain ratio(F:G) from d 29 to 56(P < 0.05), increased mortality in different growth periods(P < 0.05), elevated the levels of serum cortisol, a1-acid glycoprotein(a1-AGP), interleukin-6(IL-6) on d 24 and 25(P < 0.05), reduced immune organ indexes and contents of jejunal mucosal secretory immunoglobulin A(s Ig A) on d 28(P < 0.05). The decreased FI and BWG, as well as increased F:G and mortality in LPS-challenged birds, were alleviated by dietary L-theanine or antibiotic from d 29 to 56 and from d 1 to 56. Dietary L-theanine mitigated the elevated serum a1-AGP level on d 25, serum IL-6 concentration on d 24 and 26, and the decreased jejunal mucosal s Ig A content on d 28 of the LPS-challenged birds. The results indicated that L-theanine had potential to alleviate LPS-induced immune stress in broilers.

Dietary supplementation with pioglitazone hydrochloride and L-carnosine improves the growth performance,muscle fatty acid profiles and shelf life of yellow-feathered broiler chickens

The present study aimed to investigate the effects of dietary pioglitazone hydrochloride(PGZ)and L-carnosine(LC)supplementation on the growth performance,meat quality,antioxidant status,and meat shelf life of yellow-feathered broiler chickens.Five hundred broiler chickens were randomly assigned into 4 experimental diets using a 2×2 factorial arrangement with 2 PGZ supplemental levels(0 and 15 mg/kg)and 2 LC supplemental levels(0 and 400 mg/kg)in basal diets for 28 d.The feed-to-gain ratio decreased whereas the average daily gain increased with PGZ supplementation.Greater dressing per-centages,contents of intramuscular fat(IMF)in breast and thigh muscles,C18:3n-6,C18:1n-9 and monounsaturated fatty acid(MUFA)percentages of thigh muscle were observed with PGZ addition.Additionally,significant synergistic effects between PGZ and LC on the C18:1n-9 and MUFA contents were found.Supplementation with LC decreased drip loss,cooking loss and total volatile basic nitrogen,and increased the redness(a*)value,the superoxide dismutase and glutathione peroxidase activities in thigh muscles.Moreover,the malondialdehyde content decreased when diets were supplemented with LC,and there was a synergistic effect between PGZ and LC.Additionally,the mRNA abundance of lipogenesis-related genes,such as peroxisome proliferator-activated receptor y(PPARγ),PPARγco-activator 1αand fatty acid-binding protein 3,increased with PGZ supplementation,and relevant anti-oxidation genes,such as nuclear factor erythroid-2-related factor 2 and superoxide dismutase 1,were enhanced with LC supplementation.In conclusion,the results indicated that the supplementation of PGZ and LC could improve the growth performance,antioxidant ability,IMF content,and meat shelf life of yellow-feathered broiler chickens.

基于响应面法结合总评归一法多指标优选蜜黄芪微波炮制工艺

基于响应面法结合总评归一法,优选蜜黄芪的微波炮制工艺。在单因素实验基础上,以蜜黄芪中总黄酮、总皂苷提取率、水溶性浸出物含量和饮片外观性状的总评归一值为评价指标,对蜂蜜用量、闷润时间、炮制时间和微波火力4个因素进行的响应面法优化实验研究。最终获得最佳工艺条件为:炮制时间81 s、蜂蜜用量34%、微波火力82%、闷润时间6.1 h,此时总黄酮、皂苷、水溶性浸出物含量分别为0.3510%、0.5894%、24.71%,饮片外观评分为90。本研究优选得到的微波蜜黄芪炮制工艺合理、稳定、可行,可为蜜黄芪的工业化生产提供理论参考依据,也为其他中药的炮制提供新的研究思路和研究方法。

适用于高强度辐射场构建的多模反射面天线

高强度辐射场构建系统是对多种武器进行电磁辐照效应试验的关键装备,其要求在距天线一定距离的区域内产生高强度且尽量均匀分布的电磁场。本文设计了一款适用于该系统的X波段偏置卡塞格伦多模反射面天线。用反射面天线是为了获得尽可能高的增益,使期望区域内的场强尽可能大;用多模反射面理论实现了窄波束的平顶赋形,使期望区域内的场趋于均匀分布,区域外的场迅速减小。实测结果表明天线增益大于29.8 dB,3 dB波束宽度不小于4.6°,在此范围内方向图幅度起伏小于2 dB,平顶特性明显。此外,偏置的反射面天线还有馈源遮挡小、馈线损耗低和易于折叠收藏等优点,可以很好的应用于电磁环境模拟试验设备中。

环磷酰胺诱导黄羽肉鸡免疫抑制的研究

180只墟岗黄雏鸡随机分为4组,第1组为对照组,第2至4组为处理组,10日龄开始,对照组胸肌注射0 5mL的生理盐水,第2至4组分别按40、80和160mg/kg的剂量,同部位注射用生理盐水稀释的环磷酰胺(cyclophos phamide,CY)0 5mL;1次/d,连续注射3d.21、35、56日龄时每组随机抽取10只鸡进行屠宰取样;测定各组鸡的器官指数.另取3只鸡,测定脾脏淋巴细胞增殖转化和脾脏抗体形成细胞的数量.试验结果表明:一定剂量的环磷酰胺能显著降低墟岗黄鸡早期的体质量,且效果与注射剂量呈依赖关系;降低鸡早期脾脏指数和法氏囊指数;显著减少由ConA诱导的脾脏淋巴细胞的增殖转化;减少鸡早期脾脏抗体形成细胞的数量.

复方中药对肉鸡免疫功能和抗氧化功能的影响研究

笔者自拟一剂中药(炙黄芪200 g、当归160 g、何首乌120 g、淫羊藿120 g、扁豆100 g、黄柏20 g、穿心莲20 g、附子160 g、炙甘草100 g),以艾维茵肉鸡为试验动物,研究其对禽免疫功能和抗氧化功能的影响。试验结果表明,试验各组与对照组比较,免疫器官指数、淋巴细胞转化率、血清中抗体水平3项指标均有显著差异(P<0.05);血液中超氧化物歧化酶(SOD)的活性和总抗氧化能力2项指标也有显著差异(P<0.05)。

传统中式烤、炸、煎工艺对牛肉营养品质的影响

通过调控温度和时间,分别对牛肉进行烤、炸、煎3种高温处理,筛选出较优的高温烹饪条件,为消费者选择营养物质含量较高的烹饪工艺提供理论参考。研究测定了肉样的水分、粗蛋白质、粗脂肪及脂肪酸质量分数,从而综合评价其营养品质。结果表明,烤、炸、煎3种传统中式高温处理方式对牛肉营养品质影响显著(P<0.05)综合分析发现,在160℃下烤制40 min以及在226~228℃下炸制3 min、煎制2~3 min的牛肉具有较高营养品质。

硼对鸡10项生理生化指标的影响

300羽1日龄固始鸡随机分为4组,探讨在饮水中补充硼对鸡10项生理生化指标的影响,为研究硼的生物学作用提供科学依据。结果表明,补硼鸡体温、心跳和呼吸次数无明显变化;全血硼含量增加;红、白细胞数、血红蛋白含量和血清钙、磷、镁随着补硼量的增加而降低。

热应激对肉鸡抗氧化能力及腓肠肌纤维类型的影响

【目的】研究慢性热应激对黄羽肉鸡肝脏和肌肉的抗氧化能力与内质网应激以及腓肠肌肌纤维类型的影响.【方法】试验选用20只47日龄黄羽肉鸡,随机分为常温组和慢性热应激组,每组10只.鸡只饲养在人工气候室,试验期30 d.试验结束采集血液、肝脏和腓肠肌样品,利用试剂盒法检测血清生化指标、血清激素以及血清和肝脏抗氧化指标.利用实时荧光定量PCR法检测内质网应激(Endoplasmic reticulum stress,ERS)相关基因和腓肠肌不同肌纤维肌球蛋白重链(Myosin heavy chain,My HC)基因mRNA的表达.【结果和结论】慢性热应激显著降低肉鸡平均日增质量、血清甘油三酯(TG)、甲状腺激素(T3和T4)、胰岛素样生长因子1(Insulin-like growth factor 1,IGF-1),显著升高血清皮质酮(CORT)水平(P<0.05).同时,慢性热应激显著升高血清中丙二醛(MDA)含量,降低血清和肝脏谷胱甘肽过氧化物酶(GSH-PX)活性和肝脏总抗氧化能力(T-AOC)(P<0.05).此外,慢性热应激显著提高肉鸡肝脏和腓肠肌中热休克蛋白70(Heat shock protein 70,HSP70)基因以及腓肠肌中转录激活因子4(Activating transcription factor 4,ATF4)基因mRNA的表达(P<0.05),降低腓肠肌中葡萄糖调节蛋白78(Glucose-regulated protein 78,GRP78)基因mRNA的表达,而对肝脏中ATF4和GRP78基因mRNA的表达量无显著影响.最后,慢性热应激显著降低腓肠肌慢肌(Slow myofiber,SM)My HC基因mRNA表达量(P<0.05)而对快红肌(Fast red myofiber,FRM)和快白肌(Fast white myofiber,FWM)My HC基因mRNA表达量无影响.研究结果提示,慢性热应激可能通过降低血清生长和代谢激素、机体抗氧化能力以及引发细胞内质网应激影响肉鸡生长和腓肠肌纤维类型.

葡萄糖酸对肉鸡生产性能及养分表观消化率的影响

选取1日龄罗斯308肉仔鸡540只,随机分为6个处理,每个处理6个重复,每个重复15只。处理Ⅰ为对照组,饲喂基础日粮,处理Ⅱ、Ⅲ、Ⅳ、Ⅴ和Ⅵ在基础日粮中分别添加0.08%、0.15%、0.20%、0.30%和0.40%葡萄糖酸(GA)。研究葡萄糖酸对肉鸡生产性能及营养物质表观消化率的影响,探讨葡萄糖酸在肉鸡日粮中应用的可行性。结果表明:①与对照组相比,0.15%和0.20%添加组显著提高0～21日龄、22～42日龄鸡的日增重(P<0.05);0.20%添加组降低0～21日龄料重比2.67%(P<0.05),0.15%添加组降低22～42日龄料重比1.72%(P<0.05),0.15%和0.20%添加组分别降低试验0～42日龄料重比1.89%和1.61%,达到显著水平(P<0.05);GA对肉鸡日采食量无显著影响(P>0.05)。②21日龄时,0.15%～0.30%添加组显著提高粗蛋白和钙的表观消化率(P<0.05),0.15%和0.20%添加组显著提高磷的表观消化率(P<0.05);42日龄时,0.15%、0.20%和0.40%添加组均显著提高粗蛋白和磷的表观消化率(P<0.05),0.15%～0.30%添加组显著提高钙的表观消化率(P<0.05)。

从粉煤灰中回收氧化铝

简要介绍了国外从粉煤灰中回收氧化铝的几种方法，着重研究了煅烧／稀酸过滤法（Ｃａｌｓｉｎｔｅｒ法）影响氧化铝回收率的各种因素。

中药四黄提取物对肉仔鸡小肠黏膜免疫效果的观察

本试验观察了中药四黄提取物对肉仔鸡小肠黏膜免疫的效果。试验选用204羽1日龄AA健康肉雏鸡,随机分为2组,试验组和对照组,每组102羽。中药四黄提取物按200 mg/kg添加到试验组肉鸡的基础日粮中,试验期为40 d。试验结束时,每组随机选取50羽鸡进行屠宰,采集鸡小肠组织样本。采用组织学方法观察中药四黄提取物对肉仔鸡小肠黏膜免疫的效果。结果表明,试验组鸡十二指肠黏膜内淋巴细胞分布比对照组明显增多;空肠黏膜内淋巴细胞分布比对照组更加密集;回肠黏膜和黏膜下层中淋巴细胞有明显聚集现象,且淋巴细胞数量比对照组显著增多。本试验结果说明中药四黄提取物作为饲料添加剂对肉仔鸡小肠黏膜免疫具有明显的促进作用。

饲粮代谢能和可消化赖氨酸水平对肉仔鸡排泄物化学组成及主要臭气化合物含量的影响

本试验旨在研究饲粮代谢能(ME)和可消化赖氨酸(DLys)水平对肉仔鸡排泄物的化学组成及主要臭气化合物含量的影响。试验采用2×3两因子交叉试验设计,包括2个ME水平:12.55、13.18 MJ/kg,3个DLys水平:0.80%、0.95%、1.10%,依据理想氨基酸平衡模式配制6种试验饲粮。选取360只21日龄爱拔益加(AA)肉仔鸡,随机分为6组,每组6个重复,每个重复10只鸡。试验期21 d。结果表明:1)高ME低DLys组肉仔鸡排泄物苏氨酸(Thr)、缬氨酸(Val)、蛋氨酸(Met)、亮氨酸(Leu)和氮(N)含量最低,高ME中DLys组异亮氨酸(Ile)、赖氨酸(Lys)、组氨酸(His)、精氨酸(Arg)含量最低,低ME低DLys组总能(GE)、磷(P)含量最低。2)肉仔鸡排泄物挥发性盐基氮与总有机酸含量随饲粮ME水平的升高而增加。ME与DLys的互作效应对挥发性脂肪酸(VFA)含量有极显著影响(P<0.01),低ME组VFA含量随DLys水平的升高而增加,高ME组VFA含量随DLys水平升高而降低。pH随ME、DLys水平的升高而降低。综上所述,在本试验条件下,有利于减少肉仔鸡排泄物N、P、挥发性盐基氮等主要臭气化合物含量的适宜ME和DLys水平分别为12.55 MJ/kg和0.80%(ME/DLys=1.549 MJ/g)或13.18 MJ/kg和0.80%(ME/DLys=1.198 MJ/g)。

白鸭禽流感H5亚型疫苗免疫促进剂的筛选

选用禽源干扰素、黄芪多糖、人参皂苷、禽用抗菌肽、蜂灵散和植物血细胞凝集素分别按常用剂量单一及两两组合在接种疫苗前后3d连续喂服3次,对14d首免(0.3mL/只)、21d二免(0.6mL/只)禽流感H5亚型疫苗的白鸭进行免疫促进试验。结果显示,各免疫组雏鸭H5AI HI抗体于第6周龄达到峰值,单独作用组分别为8.0log2、7.3log2、7.73log2、9.0log2、9.18log2、9.0log2,免疫对照组为7.75log2。两两组合作用组抗体在6～7周龄达到峰值的7.3log2～9.6log2,其中干扰素+蜂灵散组、干扰素+黄芪多糖组、人参皂苷+抗菌肽组抗体峰值比单用组高1log2～2log2,高于免疫对照组峰值近2个滴度(9.64∶7.75)。试验结果表明,单一促免试验,以蜂灵散、抗菌肽、血凝素三种免疫促进效果最好,高于免疫对照组1个滴度以上。组合免促,以干扰素+蜂灵散效果最好,植物血凝素、蜂灵散单一免促组与之相近。试验初步筛选出了效果较为明显的雏鸭禽流感疫苗免疫促进剂,为以后的进一步研究及应用奠定了基础。

乳腺癌组织中Bmi1的表达及意义

目的检测乳腺癌组织中Bmi1蛋白的表达,探讨Bmi1蛋白的表达与乳腺癌临床病理特征相关性。方法收集79例乳腺癌组织标本,利用免疫组化的方法检测Bmi1和E-cadherin的表达,分析Bmi1蛋白的表达与乳腺癌临床病理特征和E-cadherin的表达相关性。结果 Bmi1蛋白在乳腺癌中的表达率约31.6%(25/79),Bmi1蛋白高表达与淋巴结的转移呈正相关;Bmi1蛋白的表达与年龄、肿瘤大小、组织学分级无关。E-cadherin的表达率为57.0%(45/79),Bmi1蛋白的表达与E-cadherin的表达呈负相关。结论乳腺癌组织中Bmi1蛋白的表达与乳腺癌的转移有关,有可能成为预测乳腺癌发生上皮间质转化的新分子标记物。

炙甘草汤对实验性自身免疫性心肌炎的治疗作用及机制研究

目的探讨炙甘草汤对实验性自身免疫性心肌炎的疗效及可能的作用机制。方法40只BALB/c鼠随机分成空白对照组、实验性自身免疫性心肌炎(EAM)组、炙甘草汤组及强的松组各10只。采用重组α肌球蛋白重链多肽(MyHC-α)诱导小鼠制备实验性自身免疫性心肌炎(EAM)模型。将弗氏佐剂与MyHC-α等体积混合,分别在第0 d和第7 d双足底注射于EAM组、炙甘草汤组和强的松组的小鼠,空白对照组注射等体积的弗氏佐剂。从第8 d起通过灌胃的方式给予炙甘草汤组小鼠炙甘草汤(22.76 g/kg,1/d),强的松组小鼠给予强的松(7.6 mg/kg,1/d),空白对照组和EAM组小鼠给予等体积生理盐水。持续给药14 d后利用HE染色观察各组小鼠心肌组织的病理性改变。流式液相多重蛋白定量技术检测心肌组织匀浆液中细胞因子干扰素γ(IFN-γ)、白介素4(IL-4)、白介素17A(IL-17A)、白介素10(IL-10)、白介素1α(IL-1α)、白介素6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;流式细胞术检测各组脾脏CD4+T细胞亚群分布。结果EAM组的小鼠在免疫后心肌组织切片HE染色表现为典型的心肌炎改变,心内膜表面变色和广泛的心肌炎症细胞浸润。EAM组中小鼠心肌组织匀浆细胞因子IFN-γ、IL-1α、IL-6水平均较空白对照组有显著升高,差异有统计学意义(P<0.05);脾脏中辅助性T细胞1(Th1)、辅助性T细胞17(Th17)水平在免疫后较空白对照组也有显著升高(P<0.05),但辅助性T细胞2(Th2)水平变化不明显。炙甘草汤组能显著减轻心肌组织的炎症反应,心脏中细胞因子IFN-γ、IL-6、IL-1α水平较EAM组均显著降低(P<0.05),脾脏中Th1、Th17细胞水平较EAM组也显著下降(P<0.05)。结论炙甘草汤在EAM发展中能够减轻炎症反应,通过减少活化的炎症细胞及抑制心肌中的炎症细胞因子分泌来对抗EAM,改善心肌炎症反应,降低心肌损伤。

气相色谱-质谱联用技术分析续断及其酒灸品中挥发性成分

目的:分析续断及其酒灸品中挥发性成分的组成和含量。方法:采用顶空固相微萃取法(HS-SPME)结合气质联用技术(GC-MS)对续断及其炮制品挥发性成分进行分析鉴定,用面积归一化法计算各成分的相对百分含量。结果:共鉴定出31种成分,主要为酚类、烯类、醇类和醛类。从续断挥发性成分中分离出25个峰,鉴定出22个成分,占总挥发性成分的94. 74%,含量最高的是丁香酚,占总挥发性成分的28. 37%;从酒灸品中分离出23个峰,鉴定出17种成分,占总挥发性成分的85. 92%,含量最高的为壬醛,占总挥发性成分的9. 66%。结论:续断及其酒灸品的挥发性成分组成和含量差异较大,本试验为续断及其酒灸品挥发性成分进一步研究开发提供科学参考依据。

胺碘酮联合炙甘草汤、养心复律汤治疗心律失常的应用价值

目的:探讨胺碘酮联合炙甘草汤、养心复律汤治疗心律失常的应用价值。方法:回顾性分析本院2018年1月-2019年1月收治的120例心律失常患者病例资料,按治疗方式不同分对照组与研究组,每组60例。对照组接受胺碘酮治疗,研究组接受胺碘酮联合炙甘草汤、养心复律汤治疗。比较两组治疗前后中医症状积分、动态心电图及心功能指标。结果:治疗后,研究组各项中医症状积分均低于对照组,差异均有统计学意义(P<0.05);治疗后,研究组短阵室速数、三联律数目、二联律数目及室早总数均少于对照组,差异均有统计学意义(P<0.05);治疗后,研究组各项心功能指标均优于对照组,差异均有统计学意义(P<0.05)。结论:胺碘酮联合炙甘草汤、养心复律汤可显著改善心律失常患者临床症状与心功能指标,减低心律失常发作次数,效果明显,值得临床推广。