Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical ...Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical antibiotic-resistant H. pylori strains were isolated and incubated in liquid medium containing Jinghua Weikang Capsule or Chenopodium ambrosioides L. By means of time-kill curve method, the average colony counts and bactericidal rate were calculated at time points of 0, 4, 8 and 24 h after the incubation and the time-kill curves were charted. Results： Both Jinghua Weikang Capsule and Chenopodium ambrosioides L. at a concentration of 0.64 g/L showed obvious bactericidal effect against antibiotic-resistant H. pylori after 4 h of incubation. Conclusion： Jinghua Weikang Capsule and Chenopodium ambrosioides L. are considered to be active against antibiotic-resistant H. pylori in vitro.展开更多
OBJECTIVE: To investigate the inhibitory effect of Jinghua Weikang capsule(JWC) on gastric inflammation induced by Helicobacter pylori(H. pylori)via the nuclear factor-kappa B(NF-κB) signaling pathway in Kunming mice...OBJECTIVE: To investigate the inhibitory effect of Jinghua Weikang capsule(JWC) on gastric inflammation induced by Helicobacter pylori(H. pylori)via the nuclear factor-kappa B(NF-κB) signaling pathway in Kunming mice.METHODS: We investigated the anti-inflammation potential of JWC extract in vivo in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and m RNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.RESULTS: JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBαand NF-κB p65, m RNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.CONCLUSION: An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo.展开更多
基金Supported by Capital Medical Development and Research Foundation(No.SF-2007-Ⅲ-01)National Natural Science Foundation of China(No.8107295)
文摘Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical antibiotic-resistant H. pylori strains were isolated and incubated in liquid medium containing Jinghua Weikang Capsule or Chenopodium ambrosioides L. By means of time-kill curve method, the average colony counts and bactericidal rate were calculated at time points of 0, 4, 8 and 24 h after the incubation and the time-kill curves were charted. Results： Both Jinghua Weikang Capsule and Chenopodium ambrosioides L. at a concentration of 0.64 g/L showed obvious bactericidal effect against antibiotic-resistant H. pylori after 4 h of incubation. Conclusion： Jinghua Weikang Capsule and Chenopodium ambrosioides L. are considered to be active against antibiotic-resistant H. pylori in vitro.
基金Supported by Grants from the National Natural Science Foundation of China Project:Research on the Anti-inflammatory Effects and Mechanism of Jinghua Weikang Capsule and Its Ingredients Against H.pylori Infected Gastritis through TLR4-NFKB-cytokine Pathway in Mice(No.81473474)Natural Science Foundation of Beijing Project:the Effectand Mechanism of Jinghua Weikang Capsule Inhibiting the Adhesion Effect of H.pylori(No.7172220)
文摘OBJECTIVE: To investigate the inhibitory effect of Jinghua Weikang capsule(JWC) on gastric inflammation induced by Helicobacter pylori(H. pylori)via the nuclear factor-kappa B(NF-κB) signaling pathway in Kunming mice.METHODS: We investigated the anti-inflammation potential of JWC extract in vivo in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and m RNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.RESULTS: JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBαand NF-κB p65, m RNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.CONCLUSION: An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo.
