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Effects of H pylori infection on gap-junctional intercellular communication and proliferation of gastric epithelial cells in vitro 被引量:8
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作者 Ran Tao Miao-Feng Hu Jin-Tu Lou Yong-Liang Lei 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第41期5497-5500,共4页
AIM: To explore the effects of H pylori infection on gap-junctional intercellular communication (GJIC) and proliferation of gastric epithelial cells in vitro. METHODS: A human gastric epithelial cell line (SGC- 7... AIM: To explore the effects of H pylori infection on gap-junctional intercellular communication (GJIC) and proliferation of gastric epithelial cells in vitro. METHODS: A human gastric epithelial cell line (SGC- 7901) cultured on coverslips was exposed overnight to intact H pylori (CagA^+ or CagA^- strains) and sonicated extracts, respectively. GJIC between the cells was detected by fluorescence redistribution after photobleaching (FRAP) technique. Proliferation of SGC cells was determined by methylthiazolyl tetrazolium (MTT) assay. RESULTS: When compared with control in which cells were cultured with simple medium alone, both CagA^+ and CagA^- H pylori isolates could inhibit GJIC (CagA^+: F = 57.98, P 〈 0.01; CagA^-: F = 29.59, P 〈 0.01) and proliferation (CagA^+: F = 42.65, P 〈 0.01; CagA^-: F = 58.14, P 〈 0.01) of SGC-7901 cells. Compared with CagA^- strains, CagA^+ H pylori more significantly downregulated GJIC of gastric cells (intact Hpylori: t = 13.86, P 〈 0.01; sonicated extracts: t = 11.87, P 〈 0.01) and inhibited proliferation gastric cells to a lesser extent in vitro (intact H pylori: t = 3.06, P 〈 0.05; sonicated extracts: t = 3.94, P 〈 0.01). CONCLUSION: Compared with CagA^- H pylori strains, CagA^+ strains down-regulate GJIC of gastric epithelial cells more significantly and inhibit proliferation of gastric cells to a lesser extent in vitro. H pylori, especially CagA^+ strains, may play an important role in gastric carcinogenesis. 展开更多
关键词 HPYLORI Gap-junctional intercellular communication Gastric epithelial cell CAGA Fluorescence redistribution after photobleaching Methylthiazolyl tetrazolium assay
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THE EFFECT OF ALL-TRANS RETINOIC ACID ON GAP JUNCTIONAL INTERCELLULARCOMMUNICATION AND CONNEXIN 43 GENE EXPRESSION IN GLIOMA CELLS 被引量:5
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作者 张雪峰 任祖渊 +4 位作者 左瑾 苏长保 王任直 常永生 方福德 《Chinese Medical Sciences Journal》 CAS CSCD 2002年第1期22-26,共5页
To illuminate the regulating effect of all trans retinoic acid (ATRA ) on gap junctional intercellular communication (GJIC) and connexin 43 (Cx43) ge ne expression in glioma cells, which is tissue and organ specific. ... To illuminate the regulating effect of all trans retinoic acid (ATRA ) on gap junctional intercellular communication (GJIC) and connexin 43 (Cx43) ge ne expression in glioma cells, which is tissue and organ specific. Method. Rat C6 glioma cells were exposed to ATRA at a concentration of 1, 10, 10 0 μmol/L respectively, and the GJIC function of the cells was examined with scr ape loading dye transfer assay 24 hours, 48 hours and 72 hours after ATRA treat ment. The effect of ATRA on Cx43 gene expression was measured with semiquantitat ive reverse transcription polymerase chain reaction (RT PCR) 24 hours after ATR A exposure. Results. The GJIC function of C6 glioma cells was significantly increased by ATR A at each concentration applied. The dye passed 4 to 5 rows of cells from the sc raping edge in ATRA treated cells, but only 1 or 2 rows in the control. The augm ent effect was observed 24 hours after each concentration ATRA treatment, and la sted till 72 hours after treatment with 1μmol/L and 10μmol/L ATRA. Forty eigh t hours after exposed to 100μmol/L ATRA, the enhancement of GJIC was less obvi ous. There was no significant increase induced by ATRA on the transcription of C x43 gene, as demonstrated by semiquantitative RT PCR. Conclusion. ATRA turned out to be a potent enhancer on GJIC function in C6 gliom a cells, and the enhancement effect was most probable at post transcriptional l evel. 展开更多
关键词 all trans retinoic acid gap junctional intercellular communication connexin 43 GLIOMA
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Exosomes rewire the cartilage microenvironment in osteoarthritis:from intercellular communication to therapeutic strategies 被引量:10
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作者 Yuangang Wu Jiao Li +5 位作者 Yi Zeng Wenchen Pu Xiaoyu Mu Kaibo Sun Yong Peng Bin Shen 《International Journal of Oral Science》 SCIE CAS CSCD 2022年第3期259-274,共16页
Osteoarthritis(OA)is a prevalent degenerative joint disease characterized by cartilage loss and accounts for a major source of pain and disability worldwide.However,effective strategies for cartilage repair are lackin... Osteoarthritis(OA)is a prevalent degenerative joint disease characterized by cartilage loss and accounts for a major source of pain and disability worldwide.However,effective strategies for cartilage repair are lacking,and patients with advanced OA usually need joint replacement.Better comprehending OA pathogenesis may lead to transformative therapeutics.Recently studies have reported that exosomes act as a new means of cell-to-cell communication by delivering multiple bioactive molecules to create a particular microenvironment that tunes cartilage behavior.Specifically,exosome cargos,such as noncoding RNAs(ncRNAs)and proteins,play a crucial role in OA progression by regulating the proliferation,apoptosis,autophagy,and inflammatory response of joint cells,rendering them promising candidates for OA monitoring and treatment.This review systematically summarizes the current insight regarding the biogenesis and function of exosomes and their potential as therapeutic tools targeting cell-to-cell communication in OA,suggesting new realms to improve OA management. 展开更多
关键词 Exosomes rewire the cartilage microenvironment in osteoarthritis from intercellular communication to therapeutic strategies
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Altered Expression of Connexin-43 and Impaired Capacity of Gap Junctional Intercellular Communication in Prostate Cancer Cells 被引量:6
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作者 邢毅飞 肖亚军 +4 位作者 曾甫清 赵军 肖传国 熊平 冯玮 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第3期291-294,共4页
Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-ca... Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elu- cidate the reason why the so-called 'bystander effect' mediated by thymidine kinase (TK) suicide gene therapy on PCa cells is not of significance and to explore the role of GJIC in PCa carcinogenesis. mRNA and protein expression of Cx43 in a PCa cell line PC-3m was detected by re- verse-transcription polymerase chain reaction (RT-PCR) and strapt-avidin-biotin-enzyme complex (SABC) immunohistochemical staining, and inherent GJIC of PC-3m cells was assayed by scrape-loading and dye transfer (SLDT) assay. The expression of Cx43 in human normal and malig- nant prostate tissues was determined by SABC immunohistochemistry as well. It was found that Cx43 mRNA and protein expression in PC-3m cells was slightly reduced as compared with positive controls and the location of Cx43 protein was aberrant in cytoplasm rather than on membrane. As- sessment of paraffin sections demonstrated that the expression of Cx43 protein in PCa cells was ab- normally located and markedly diminished as compared with normal prostatic epithelial ones, dis- playing a negative correlation to the pathological grade (χ2=4.025, P<0.05). Additionally, capacity of inherent GJIC in PC-3m cells was disrupted, which was semi-quantified as (+) or (-). It was indi- cated that both down-regulated expression of Cx43 mRNA and aberrant location of Cx43 protein par- ticipated in the mechanisms leading to deficient GJIC in PC-3m cells. Lack of efficient GJIC is a molecular event, which may contribute not only to limited extent of 'bystander effect', but also to initiation and progression of prostatic neoplasm. 展开更多
关键词 prostate neoplasms gap junctional intercellular communication herpes simplex virus thymidine kinase gene/ganciclovir CONNEXIN bystander effect
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Up-Regulation of the Gap Junction Intercellular Communication by Tea Polyphenol in the Human Metastatie Lung Carcinoma Cell Line 被引量:3
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作者 Xiangyong Li Qinghua Wang +6 位作者 Jun Yang Yanjuan Pan Qingyong Chen Xiqing Yan Daxin Wang Xijian Zhou Yuquan Wu 《Journal of Cancer Therapy》 2012年第1期64-70,共7页
Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic h... Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC. 展开更多
关键词 Tea POLYPHENOL LUNG Neoplasms Highly METASTATIC HUMAN LUNG Carcinoma Cell Line Gap Junction intercellular communication
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Intercellular communication of notochord cells during their differentiation in Cynops orientalis
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作者 ZENGMIBAI YINGWANG 《Cell Research》 SCIE CAS CSCD 1993年第2期141-145,共5页
Intercellular communication of notochord cells during their differentiation was studied by microinjection of a fluorescent dye, Lucifer Yellow. Close correlation existed between the incidences of dye coupling and quan... Intercellular communication of notochord cells during their differentiation was studied by microinjection of a fluorescent dye, Lucifer Yellow. Close correlation existed between the incidences of dye coupling and quantitative evaluation of gap junctions. High incidences of dye coupling and of gap junctions occurred at a stage when notochord cells were active in the change of cell shape and cell arrangement. With the subsidence of cell movements, both dye coupling and gap junctions were reduced to lower levels. It was, therefore, suggested that intercellular communication via gap junctions played an important role in the coordination of notochord cell movements.Gap junctions of altered configuration occurred in notochord cells in late tailbud stage. The comparison of incidences of dye coupling at this stage with those at other stages strongly suggested that the gap junctions of altered configuration functioned just as those of generalized type. 展开更多
关键词 intercellular communication gap junction notochord cell.
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STUDIES ON THE GAP JUNCTIONAL INTERCELLULARCOMMUNICATION OF HUMAN NASOPHARYNGEALCARCINOMA CELLS AND THE EFFECT OF RII
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作者 韩立群 高进 +3 位作者 董化一 赵天德 高福云 余都 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1996年第1期27-31,共5页
Human nasopharyngeal carcinoma(NPC) cell line,CNE-2Z, and its clones(L2, H2, L4) with various invasive and metastatic potentials were examined for their gap junctions(GJ), gap junctional intercellular communication(GJ... Human nasopharyngeal carcinoma(NPC) cell line,CNE-2Z, and its clones(L2, H2, L4) with various invasive and metastatic potentials were examined for their gap junctions(GJ), gap junctional intercellular communication(GJIC) and the concentration of cytosolic free calcium(Ca2+). Only a few intermediate junction(IJ)but no GJ structures were observed under electron microscope(EM). CNE-2Z cells showed marked JGIC,while its variants lacked this function using the scraploading dye-transfer technique(SLDT). There was lower concentration of[Ca2+]. in L2 cells(a variant with high invasive and metastatic Potential) compared to that in H2 and L4 cells(variants with medium and low invasive and metastatic Potentials, respectively). These data suggested that high invasive and metastatic potentials might be correlated with the levcl of[Ca2+]i in NPC cells.The effect of RII(4-hydroxycarbophenyl retinamide) on NPC cells also investigated, After 3-7 d of RII(10-5 M) treatment, there was no change in the number of gap junctions and other kind of intercellular junctions in NPC cells observed under EM. The JGIC of CNE-2Z weaked and then disappeared finally with prolonging of RII treatment. However. there was no influence on its variants. The level of[Ca2+], in NPC cells apparently fell after 6 h of RII treatment, and rose to original level with persisting of RII treatment. Whether the fluctuating of[Ca2+]i level is related to the inhibitory effect of RII treatment on growth and invasion of NPC cells needs to be further studied. 展开更多
关键词 Gap junctional intercellular communication(JGIC) RETINOIDS intercellular free calcium Invasion Metastasis.
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Mechanism of changes in gap junctional intercellular communication in myocardial cells after burns
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作者 迟路湘 杨宗诚 +1 位作者 王旭 黎鳌 《Journal of Medical Colleges of PLA(China)》 CAS 1999年第1期17-20,共4页
Objective: To explore the pathophysiological mechanism of the changes in gap junctionalintercellular communication (GJIC) in the myocardial cells after burns. Methods: After the myocardial cellswere cultured and injur... Objective: To explore the pathophysiological mechanism of the changes in gap junctionalintercellular communication (GJIC) in the myocardial cells after burns. Methods: After the myocardial cellswere cultured and injured with hypoxia and burn serum, the GJIC in the cells was detected with scrapeloading and dye transfer. Meanwhile, the viability, cytosolic free Ca2+ concentration and Ca2+ influx of themyocardial cells were determined. Results: The cytosolic free Ca2+ concentration and the cellulartransmembrane Ca2+ influx were significantly increased but the viability of the cells markedly decreased afterthe injury. The LY fluorescence reached 4 rows of cells from the scrape line in the normal myocardial cells.The GJIC was blocked at the first hour after hypoxia or hypoxia and burn serum injury. The LY fluorescencewas limited to the primary loads cells at the sixth hour after hypoxia and the third hour after hypoxia andburn serum injury. Conclusion: The function of GJIC in the myocardial cells is to maintain high ordersynchronous contraction of the myocardium. After burns, the runaway calcium homeostasis and impairmentof GJIC function would be accused to be the pathological basis for myocardial heterogeneous behavior. 展开更多
关键词 scrape--loading DYE transfer gap JUNCTIONAL intercellular communication calcium MYOCARDIAL cell BURN
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EFFECTS OF LIMONENE,SALVIA MILTIORRHIZA AND TURMERIC DERIVATIVES ON H-RAS ONCOGENE EXPRESSION AND GAP JUNCTION INTERCELLULAR COMMUNICATION IN HUMAN SOLID TUMOR CELL LINES
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作者 陈晓光 连间忠芳 +2 位作者 矢野善久 吉都俣士子 大谷周造 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1998年第3期8-14,共7页
Objective: To study gap junction intercellular communication (GJIC), H ras oncogene expression and ras oncogene product (P 21 ras protein) expression in four human solid tumor cell lines, W1-38,CACO 2,A549 and... Objective: To study gap junction intercellular communication (GJIC), H ras oncogene expression and ras oncogene product (P 21 ras protein) expression in four human solid tumor cell lines, W1-38,CACO 2,A549 and PaCa, and the effects of four compounds, Salvia miltiorrhiza derivative (SMD), d Limonene, Turmeric derivative I (TD I) and Turmeric derivative II (TD II), on them. Methods: The abilities of the four solid tumor cell lines to transfer dye to adjacent cells were examined by the scrape loading/dye transfer technique, and the H ras oncogene expression by Northern blotting and P 21 ras protein expression by Western blotting. Results: The results showed the loss of intercellular coupling in PaCa cells, slight GJIC in A549 and CACO 2 cells, and a good GJIC in W1-38 cells. The four compounds could improve the GJIC of PaCa to different extents. The amount of total and membrane associated P 21 ras in PaCa cells were decreased after treatment with SMD, d Limonene and TD I (2.5 μg/ml) for 48 h. Concomitantly, the growth of PaCa cells decreased in soft agar and had enhanced GJIC. The relative potency was found to be:d Limonene>SMD >TD I=TD II. There was no significant effect of the four compounds on H ras oncogene expression. Conclusion: It was suggested that there was an excellent correlation between loss of Lucifer Yellow dye transfer and ras gene mutation rate in the four solid tumor cell lines (ras gene mutation rate inversely correlated with average cell number coupled, r=0.98) i.e., the high ras gene mutation was closely correlated with loss of GJIC in these malignant human tumor cells; The antitumor effect of the monoterpene d Limonene and the phenol compound, SMD, might be related to inhibition of P 21 ras membrane association and enhancement of GJIC, whilst that of the others may be by a different mechanism; The inhibition of P 21 ras membrane association was directly related to the enhancement of gap junction intercellular com munication. 展开更多
关键词 d Limonene Salvia miltiorrhiza derivative (SMD) Turmeric derivatives H ras oncogene GAP Junction intercellular communication (GJIC).
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Changes of gap junctional intercellular communication in detrusor instability
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作者 周逢海 宋波 +1 位作者 金锡御 范立新 《Journal of Medical Colleges of PLA(China)》 CAS 2004年第1期7-10,共4页
Objective: To demonstrate the functional changes of gap junctional mediation of intercellular communication in detrusor instability (DI) and its mechanisms. Methods: The function of gap junctional intercellular commun... Objective: To demonstrate the functional changes of gap junctional mediation of intercellular communication in detrusor instability (DI) and its mechanisms. Methods: The function of gap junctional intercellular communication in the cultured bladder detrusor cells was detected by fluorescence redistribution after photobleaching. Results: At the fourth minute after bleaching, the mean fluorescences recovery rates of DI group bladder detrusor cells were (35 791±0 836)%, that of control group (8 645±0 673)%. The mean fluorescence recovery rates of DI group were significantly higher than those of control group ( P <0 01). Conclusion: It shows that the increase of intercellular excitatory communication is one of the important reasons of pathogenesis of DI. 展开更多
关键词 detrusor instability gap junctional intercellular communication fluorescence redistribution after photobleaching
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Influence of (-)-epigallocatechin-3-gallate on the expression of connexin43 and gap junction intercellular communication of the bladder cancer cell lines BIU-87 in vitro
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作者 Zhengguo Cao Chao Tian +4 位作者 Maolin Jiang Kui Wu Xiaojian Zhong Jianxin Li Yuefu Han 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第12期716-720,共5页
Objective: The aim of our study was to investigate the effects of (-)-epigallocatechin-3-gallate (EGCG, the major phytochemistry component in green tea) on the expression of connexin43 (Cx43) gene and detect th... Objective: The aim of our study was to investigate the effects of (-)-epigallocatechin-3-gallate (EGCG, the major phytochemistry component in green tea) on the expression of connexin43 (Cx43) gene and detect the intercellular communication of the human bladder cancer cell lines BIU-87, and explore its possible mechanisms of prevention and cure for the bladder tumor. Methods: The methyl thiazolyl tetrazolium and Annexin-V/PI double-labeled flow cytometry methods were used to observe the growth inhibitory rate (IR) and apoptosis rate (AR) of BlU-87 cells treated by EGCG at different concentrations (0, 5, 10 and 20 mg/L), respectively. The reverse transcription-polymerase chain reaction (RT-PCR) and Western Blotting analysis were employed to detect the relative expression levels of the Cx43 mRNA and its protein. The scrape-loading fluorescence dye transfer method was used to assess the gap junction intercellular communication (GJIC) under fluorescence microscope. Results: EGCG at concentrations (10 and 20 mg/L) both could significantly inhibit the proliferation and induce the apoptosis of BIU-87 cells. The IR and AR were (15.67 ± 1.15)%, (18.33 ± 1.53)% and (42.00 ± 4.34)%, (27.33 ± 3.21)%, respectively. And compared with the control groups of 0 mg/L and 5 mg/L (P 〈 0.05), EGCG could significantly up-regulate the expression of Cx43 mRNA and its protein and enhance the function of BIU-87 cells. The effects had the significant correlation with the dose-dependent of EGCG. Conclusion: EGCG (10, 20 mg/L) could effectively up-regulate Cx43 expression and enhance the GJIC of BlU-87 cells. The results may indicate the effects of EGCG inducing bladder tumor cells apoptosis and inhibiting its growth which provides the experimental evidence for further demonstrating the mechanism of chemical prevention and cure for the bladder tumor by EGCG. 展开更多
关键词 (-)-epigallocatechin-3-gallate bladder tumor CONNEXIN43 intercellular communication
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Differences between the gap junctional communication function of fibroblasts derived from pathological scars and normal skin 被引量:4
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作者 鲁峰 高建华 黎小间 《Journal of Medical Colleges of PLA(China)》 CAS 2000年第2期150-152,共3页
Objective: To define the mechanisms underlying the keloid formation. Methods: The gap junctional intercellu- lar commumication (GJIC) of fibroblasts derived from pathological scars and noed skin was investigated. Six ... Objective: To define the mechanisms underlying the keloid formation. Methods: The gap junctional intercellu- lar commumication (GJIC) of fibroblasts derived from pathological scars and noed skin was investigated. Six Samples of hyperplastic scars, keloids and normal skin were obtained. Fibroblasts from these samples were cultured in ho and vended by type Ⅰ collagen, and the GJIC among the fibroblasts was investigated by adherent cell analysis with soning interactive laser coytometer(ACAS 570) for fibroblasts culturing. Results: The fibroblasts from normal skin showed nounal GJIC, which are depressed between fibroblasts from hyperplastic scare and was completely blocked in fibroblasts from the keloids. Conclusion: The blockade of interoellular communication may play an important role in the pathogenesis of excessive and invasive growth of fibroblasts derived from the keloids. 展开更多
关键词 KELOID FIBROBLAST intercellular JUNCTIONAL communication
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Identification of immune feature genes and intercellular profiles in diabetic cardiomyopathy
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作者 Ze-Qun Zheng Di-Hui Cai Yong-Fei Song 《World Journal of Diabetes》 SCIE 2024年第10期2093-2110,共18页
BACKGROUND Diabetic cardiomyopathy(DCM)is a multifaceted cardiovascular disorder in which immune dysregulation plays a pivotal role.The immunological molecular mechanisms underlying DCM are poorly understood.AIM To ex... BACKGROUND Diabetic cardiomyopathy(DCM)is a multifaceted cardiovascular disorder in which immune dysregulation plays a pivotal role.The immunological molecular mechanisms underlying DCM are poorly understood.AIM To examine the immunological molecular mechanisms of DCM and construct diagnostic and prognostic models of DCM based on immune feature genes(IFGs).METHODS Weighted gene co-expression network analysis along with machine learning methods were employed to pinpoint IFGs within bulk RNA sequencing(RNA-seq)datasets.Single-sample gene set enrichment analysis(ssGSEA)facilitated the analysis of immune cell infiltration.Diagnostic and prognostic models for these IFGs were developed and assessed in a validation cohort.Gene expression in the DCM cell model was confirmed through real time-quantitative polymerase chain reaction and western blotting techniques.Additionally,single-cell RNA-seq data provided deeper insights into cellular profiles and interactions.RESULTS The overlap between 69 differentially expressed genes in the DCM-associated module and 2483 immune genes yielded 7 differentially expressed immune-related genes.Four IFGs showed good diagnostic and prognostic values in the validation cohort:Proenkephalin(Penk)and retinol binding protein 7(Rbp7),which were highly expressed,and glucagon receptor and inhibin subunit alpha,which were expressed at low levels in DCM patients(all area under the curves>0.9).SsGSEA revealed that IFG-related immune cell infiltration primarily involved type 2 T helper cells.High expression of Penk(P<0.0001)and Rbp7(P=0.001)was detected in cardiomyocytes and interstitial cells and further confirmed in a DCM cell model in vitro.Intercellular events and communication analysis revealed abnormal cellular phenotype transformation and signaling communication in DCM,especially between mesenchymal cells and macrophages.CONCLUSION The present study identified Penk and Rbp7 as potential DCM biomarkers,and aberrant mesenchymal-immune cell phenotype communication may be an important aspect of DCM pathogenesis. 展开更多
关键词 Diabetic cardiomyopathy Immune feature genes PROENKEPHALIN Retinol binding protein 7 Immune cell infiltration intercellular communication
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Fas stimulation of T lymphocytes promotes rapid intercellular exchange of death signals via membrane nanotubes 被引量:4
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作者 Peter D Arkwright Francesca Luchetti +8 位作者 Julien Tour Charlotte Roberts Rahna Ayub Ana P Morales Jose J Rodriguez Andrew Gilmore Barbara Canonico Stefano Papa Mauro Degli Esposti 《Cell Research》 SCIE CAS CSCD 2010年第1期72-88,共17页
The Fas/CD95 surface receptor mediates rapid death of various cell types, including autoreactive T cells with the potential for triggering autoimmunity. Here, we present novel aspects of Fas signalling that define a ... The Fas/CD95 surface receptor mediates rapid death of various cell types, including autoreactive T cells with the potential for triggering autoimmunity. Here, we present novel aspects of Fas signalling that define a 'social' dimension to receptor-induced apoptosis. Fas stimulation rapidly induces extensive membrane nanotube formation between neighbouring T cells. This is critically dependent on Rho GTPases but not on caspase activation. Bidirectional transfer of membrane and cytosolic elements including active caspases can be observed to occur via these nanotubes. Nanotube formation and intercellular exchanges of death signals are defective in T lymphocytes from patients with autoimmune iymphoproliferative syndrome harbouring mutations in the Fas receptor. We conclude that nanotuhemediated exchanges constitute a novel form of intercellular communication that augments the propagation of death signalling between neighbouring T cells. 展开更多
关键词 FAS intercellular communication membrane nanotubes T cells
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Single-cell manipulation by two-dimensional micropatterning
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作者 Xuehe Ma Haimei Zhang +7 位作者 Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2024年第1期45-59,共15页
Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-di... Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment mimicking.Here,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography micropatterning.Moreover,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication. 展开更多
关键词 Two-dimensional micropatterning CYTOSKELETON cell migration extracellular matrix intercellular communication gene expression
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Connexin 43-modified bone marrow stromal cells reverse the imatinib resistance of K562 cells via Ca^(2+)-dependent gap junction intercellular communication 被引量:1
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作者 Xiaoping Li Yunshuo Xiao +7 位作者 Xiaoqi Wang Ruihao Huang Rui Wang Yi Deng Jun Rao Qiangguo Gao Shijie Yang Xi Zhang 《Chinese Medical Journal》 SCIE CAS CSCD 2023年第2期194-206,共13页
Background: Imatinib mesylate (IM) resistance is an emerging problem for chronic myeloid leukemia (CML). Previous studies found that connexin 43 (Cx43) deficiency in the hematopoietic microenvironment (HM) protects mi... Background: Imatinib mesylate (IM) resistance is an emerging problem for chronic myeloid leukemia (CML). Previous studies found that connexin 43 (Cx43) deficiency in the hematopoietic microenvironment (HM) protects minimal residual disease (MRD), but the mechanism remains unknown. Methods: Immunohistochemistry assays were employed to compare the expression of Cx43 and hypoxia-inducible factor 1α (HIF-1α) in bone marrow (BM) biopsies of CML patients and healthy donors. A coculture system of K562 cells and several Cx43-modified bone marrow stromal cells (BMSCs) was established under IM treatment. Proliferation, cell cycle, apoptosis, and other indicators of K562 cells in different groups were detected to investigate the function and possible mechanism of Cx43. We assessed the Ca^(2+)-related pathway by Western blotting. Tumor-bearing models were also established to validate the causal role of Cx43 in reversing IM resistance. Results: Low levels of Cx43 in BMs were observed in CML patients, and Cx43 expression was negatively correlated with HIF-1α. We also observed that K562 cells cocultured with BMSCs transfected with adenovirus-short hairpin RNA of Cx43 (BMSCs-shCx43) had a lower apoptosis rate and that their cell cycle was blocked in G0/G1 phase, while the result was the opposite in the Cx43-overexpression setting. Cx43 mediates gap junction intercellular communication (GJIC) through direct contact, and Ca ^(2+ )is the key factor mediating the downstream apoptotic pathway. In animal experiments, mice bearing K562, and BMSCs-Cx43 had the smallest tumor volume and spleen, which was consistent with the in vitro experiments. Conclusions: Cx43 deficiency exists in CML patients, promoting the generation of MRD and inducing drug resistance. Enhancing Cx43 expression and GJIC function in the HM may be a novel strategy to reverse drug resistance and promote IM efficacy. 展开更多
关键词 Bone marrow microenvironment Connexin 43(Cx43) Gap junction intercellular communication HYPOXIA Imatinib resistance
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Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling
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作者 Min Wei Yanping Sun +10 位作者 Shouzhen Li Yunuo Chen Longfei Li Minghao Fang Ronghua Shi Dali Tong Jutao Chen Yuqian Ma Kun Qu Mei Zhang Tian Xue 《Protein & Cell》 SCIE CSCD 2023年第8期603-617,共15页
Light adaptation enables the vertebrate visual system to operate over a wide range of ambient illumination.Regulation of phototransduction in photoreceptors is considered a major mechanism underlying light adaptation.... Light adaptation enables the vertebrate visual system to operate over a wide range of ambient illumination.Regulation of phototransduction in photoreceptors is considered a major mechanism underlying light adaptation.However,various types of neurons and glial cells exist in the retina,and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic investigation.Therefore,we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in mice.The results demonstrated that,in addition to photoreceptors,other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark adaptation.Importantly,Müller glial cells(MGs)were identified as hub cells for intercellular interactions,displaying complex cell‒cell communication with other retinal cells.Furthermore,light increased the transcription of the deiodinase Dio2 in MGs,which converted thyroxine(T4)to active triiodothyronine(T3).Subsequently,light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light conditions.As cones specifically express the thyroid hormone receptor Thrb,they responded to the increase in T3 by adjusting light responsiveness.Loss of the expression of Dio2 specifically in MGs decreased the light responsive ability of cones.These results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling. 展开更多
关键词 single cell Müller glial cells intercellular communication light/dark adaptation thyroid hormone signaling
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Cisplatin-induced premature senescence with concomitant reduction of gap junctions in human fibroblasts 被引量:12
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作者 WeiZHAO ZhongXiangLIN ZhiQianZHANG 《Cell Research》 SCIE CAS CSCD 2004年第1期60-66,共7页
To examine the role of gap junctions in cell senescence,the changes of gap junctions in cisplatin-induced premature senescence of primary cultured fibroblasts were studied and compared with the replicative senescent h... To examine the role of gap junctions in cell senescence,the changes of gap junctions in cisplatin-induced premature senescence of primary cultured fibroblasts were studied and compared with the replicative senescent human fibroblasts.Dye transfer assay for gap junction function and immunofluorescent staining for connexin 43 protein distribution were done respectively. Furthermore,cytofluorimetry and DAPI fluorescence staining were performed for cell cycle and apoptosis analysis. p53 gene expression level was detected with indirect immunofluorescence. We found that cisplatin (10 mM) treatment could block cell growth cycle at G1 and induced premature senescence. The premature senescence changes included high frequency of apoptosis,elevation of p53 expression,loss of membranous gap junctions and reduction of dye-transfer capacity. These changes were comparable to the changes of replicative senescence of human fibroblasts. It was also concluded that cisplatin could induce premature senescence concomitant with inhibition of gap junctions in the fibroblasts. Loss of functional gap junctions from the cell membrane may account for the reduced intercellular communication in the premature senescent fibroblasts. The cell system we used may provide a model useful for the study of the gap junction thus promoting agents against premature senescence. 展开更多
关键词 CISPLATIN premature senescence gap junction intercellular communication connexin 43 fibroblasts.
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How does Helicobacter pylori cause gastric cancer through connexins: An opinion review 被引量:11
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作者 Huan Li Can-Xia Xu +3 位作者 Ren-Jie Gong Jing-Shu Chi Peng Liu Xiao-Ming Liu 《World Journal of Gastroenterology》 SCIE CAS 2019年第35期5220-5232,共13页
Helicobacter pylori (H. pylori) is a Gram-negative bacterium with a number of virulence factors, such as cytotoxin-associated gene A, vacuolating cytotoxin A, its pathogenicity island, and lipopolysaccharide, which ca... Helicobacter pylori (H. pylori) is a Gram-negative bacterium with a number of virulence factors, such as cytotoxin-associated gene A, vacuolating cytotoxin A, its pathogenicity island, and lipopolysaccharide, which cause gastrointestinal diseases. Connexins function in gap junctional homeostasis, and their downregulation is closely related to gastric carcinogenesis. Investigations into H. pylori infection and the fine-tuning of connexins in cells or tissues have been reported in previous studies. Therefore, in this review, the potential mechanisms of H. pylori-induced gastric cancer through connexins are summarized in detail. 展开更多
关键词 HELICOBACTER pylori CONNEXIN GAP JUNCTIONAL intercellular communications GAP junction proteins Gastric cancer Transcription factors DNA methylation Proliferation Apoptosis
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Extracellular microRNAs from the epididymis as potential mediators of cell-to-cell communication 被引量:5
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作者 Clemence Belleannee 《Asian Journal of Andrology》 SCIE CAS CSCD 2015年第5期730-736,I0006-I0007,共9页
Ribonucleic acid (RNA) was previously thought to remain inside cells as an intermediate between genes and proteins during translation. However, it is now estimated that 98% of the mammalian genomic output is transcr... Ribonucleic acid (RNA) was previously thought to remain inside cells as an intermediate between genes and proteins during translation. However, it is now estimated that 98% of the mammalian genomic output is transcribed as noncoding RNAs, which are involved in diverse gene expression regulatory mechanisms and can be transferred from one cell to another through extracellular communication. For instance, microRNAs are 22-nucleotide-long noncoding RNAs that are generated by endonuclease cleavage of precursors inside the cells and are secreted as extracellular microRNAs to regulate target cell posttranscriptional gene expression via RNA interference. We and others have shown that different populations of microRNAs are expressed in distinct regions of the human epididymis and regulate the expression of target genes that are involved in the control of male fertility as indicated by knock-out mouse models. Importantly, some microRNAs, including the microRNA-888 (miR-888) cluster that is exclusively expressed in the reproductive system of human and nonhuman primates, are released in the sperm-surrounding fluid in the epididymis via extracellular vesicles, the so-called epididymosomes. In addition to interacting with the membrane of maturing spermatozoa, these extracellular vesicles containing microRNAs communicate with epithelial cells located downstream from their release site, suggesting a role in the luminal exocrine control of epididymal functions. Apart from their potential roles as mediators of intercellular communication within the epididymis, these extracellular microRNAs are potent molecular targets for the noninvasive diagnosis of male infertility. 展开更多
关键词 Dicer EPIDIDYMIS exocrine factors extracellular vesicles intercellular communication MICRORNAS paracrine regulation posttranscriptional gene regulation seminal plasma spermatozoa
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