AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE...AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE and the Chinese Biomedical Database,13 on ADH1B and 18 on ALDH2.We performed a meta-analysis of case-control studies including 13 studies on ADH1B(cases/controls:2390/7100)and 18 studies on ALDH2(2631/6030).RESULTS:The crude odds ratio[OR(95%confidence interval)]was 2.91(2.04-4.14)for ADH1B*1/*1(vs ADH1B*2/*2)and 1.32(1.17-1.49)for ADH1B*1/*2.The crude OR for ALDH2*1/*2(vs ALDH2*1/*1)was 2.52(1.76-3.61).ADH1B*1/*1 increased the risk of esophageal cancer among never/rare[1.56(0.93-2.61)],moderate[2.71(1.37-5.35)],and heavy drinkers[3.22 (2.27-4.57)].ADH1B*1/*2 was associated with a modest risk among moderate drinkers[1.43(1.09-1.87)].ALDH2*1/*2 increased the risk among never/rare[1.28 (0.91-1.80)],moderate[3.12(1.95-5.01)],and heavy [7.12(4.67-10.86)]drinkers,and among ex-drinkers [5.64(1.57-20.25)].ALDH2*2/*2 increased the risk among drinkers[4.42(1.72-11.36)].ADH1B*1/*1 plus ALDH2*1/*2 was associated with the highest risk for heavy drinkers[12.45(2.9-53.46)].The results of the meta-regression analysis showed that the effects of ADH1B*1/*1 and ALDH2*1/*2 increased with the level of alcohol consumption.ALDH2*1/*2 was associated with a high risk among Taiwan Chinese and Japanese drinkers,as opposed to a moderate risk among drinkers in high-incidence regions of China's Mainland.ADH1B*1/*1 in heavy drinkers and ALDH2*1/*2 in moderate-toheavy drinkers was associated with similarly high risk among both men and women.CONCLUSION:ADH1B/ALDH2 genotypes affect the risk of esophageal cancer,and the risk is modified by alcohol consumption,ethnicity,and gender.展开更多
Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic pol...Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C） （WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.展开更多
Background:Aldehyde dehydrogenase(ALDH)genes constitute an important family of supergenes that play key roles in synthesizing various biomolecules and maintaining cellular homeostasis by catalyzing the oxidation of al...Background:Aldehyde dehydrogenase(ALDH)genes constitute an important family of supergenes that play key roles in synthesizing various biomolecules and maintaining cellular homeostasis by catalyzing the oxidation of aldehyde products.With climate change increasing the exposure of plants to abiotic stresses such as salt and drought,ALDH genes have been identified as important contributors to stress tolerance.In particular,they help to reduce stress-induced lipid peroxidation.Objectives:This study aims to identify and characterize members of the ALDH supergene family in Phaseolus vulgaris through a genome-wide bioinformatic analysis and investigate their role in response to abiotic stressors such as drought and salt stress.Methods:Genome-wide identification of 26 ALDH genes in P.vulgaris was performed using bioinformatics tools.The identified ALDH proteins were ana-lyzed for molecular weight,amino acid number,and exon number.Phylogenetic analysis was performed to clas-sify P.vulgaris,Arabidopsis thaliana,and Glycine max ALDH proteins into different groups.Strong links between these genes and functions related to growth,development,stress responses,and hormone signaling were identified by cis-element analysis in promoter regions.In silico expression,analysis was performed to assess gene expression levels in different plant tissues.Results:RT-qPCR results showed that the expression of ALDH genes was signif-icantly altered under drought and salt stress in beans.This study provides a comprehensive characterization of the ALDH supergene family in P.vulgaris,highlighting their potential role in abiotic stress tolerance.Conclusion:Thesefindings provide a basis for future research on the functional roles of ALDH genes in enhancing plant resis-tance to environmental stressors.展开更多
文摘AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE and the Chinese Biomedical Database,13 on ADH1B and 18 on ALDH2.We performed a meta-analysis of case-control studies including 13 studies on ADH1B(cases/controls:2390/7100)and 18 studies on ALDH2(2631/6030).RESULTS:The crude odds ratio[OR(95%confidence interval)]was 2.91(2.04-4.14)for ADH1B*1/*1(vs ADH1B*2/*2)and 1.32(1.17-1.49)for ADH1B*1/*2.The crude OR for ALDH2*1/*2(vs ALDH2*1/*1)was 2.52(1.76-3.61).ADH1B*1/*1 increased the risk of esophageal cancer among never/rare[1.56(0.93-2.61)],moderate[2.71(1.37-5.35)],and heavy drinkers[3.22 (2.27-4.57)].ADH1B*1/*2 was associated with a modest risk among moderate drinkers[1.43(1.09-1.87)].ALDH2*1/*2 increased the risk among never/rare[1.28 (0.91-1.80)],moderate[3.12(1.95-5.01)],and heavy [7.12(4.67-10.86)]drinkers,and among ex-drinkers [5.64(1.57-20.25)].ALDH2*2/*2 increased the risk among drinkers[4.42(1.72-11.36)].ADH1B*1/*1 plus ALDH2*1/*2 was associated with the highest risk for heavy drinkers[12.45(2.9-53.46)].The results of the meta-regression analysis showed that the effects of ADH1B*1/*1 and ALDH2*1/*2 increased with the level of alcohol consumption.ALDH2*1/*2 was associated with a high risk among Taiwan Chinese and Japanese drinkers,as opposed to a moderate risk among drinkers in high-incidence regions of China's Mainland.ADH1B*1/*1 in heavy drinkers and ALDH2*1/*2 in moderate-toheavy drinkers was associated with similarly high risk among both men and women.CONCLUSION:ADH1B/ALDH2 genotypes affect the risk of esophageal cancer,and the risk is modified by alcohol consumption,ethnicity,and gender.
基金supported by National Natural Science Foundation of China(No.81301676)the PhD Start-up Program of Guangdong Province Natural Science Fund(No.S2012040008047)
文摘Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C） （WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.
文摘Background:Aldehyde dehydrogenase(ALDH)genes constitute an important family of supergenes that play key roles in synthesizing various biomolecules and maintaining cellular homeostasis by catalyzing the oxidation of aldehyde products.With climate change increasing the exposure of plants to abiotic stresses such as salt and drought,ALDH genes have been identified as important contributors to stress tolerance.In particular,they help to reduce stress-induced lipid peroxidation.Objectives:This study aims to identify and characterize members of the ALDH supergene family in Phaseolus vulgaris through a genome-wide bioinformatic analysis and investigate their role in response to abiotic stressors such as drought and salt stress.Methods:Genome-wide identification of 26 ALDH genes in P.vulgaris was performed using bioinformatics tools.The identified ALDH proteins were ana-lyzed for molecular weight,amino acid number,and exon number.Phylogenetic analysis was performed to clas-sify P.vulgaris,Arabidopsis thaliana,and Glycine max ALDH proteins into different groups.Strong links between these genes and functions related to growth,development,stress responses,and hormone signaling were identified by cis-element analysis in promoter regions.In silico expression,analysis was performed to assess gene expression levels in different plant tissues.Results:RT-qPCR results showed that the expression of ALDH genes was signif-icantly altered under drought and salt stress in beans.This study provides a comprehensive characterization of the ALDH supergene family in P.vulgaris,highlighting their potential role in abiotic stress tolerance.Conclusion:Thesefindings provide a basis for future research on the functional roles of ALDH genes in enhancing plant resis-tance to environmental stressors.
