Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilate...Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P<0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P<0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in shortside mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.展开更多
胃肠道Cajal间质细胞（interstitialcells of Cajal，ICC）被称为胃肠动力的起搏者和调节者，是以刚状结构存在于胃肠道平滑肌层之间的一种特殊间质细胞，不同于平滑肌细胞和神经细胞,但具有神经信息传送给平滑肌细胞的功能，即肠神经...胃肠道Cajal间质细胞（interstitialcells of Cajal，ICC）被称为胃肠动力的起搏者和调节者，是以刚状结构存在于胃肠道平滑肌层之间的一种特殊间质细胞，不同于平滑肌细胞和神经细胞,但具有神经信息传送给平滑肌细胞的功能，即肠神经系统释放的神经递质和肽类物质与ICC上的受体结合，激发ICC去极化，调控胃肠道平滑肌的舒缩功能。展开更多
基金supported by the Beijing Featured Livestock and Poultry Genetic Resources Preservation Project,China(202203310002)China Agriculture Research System of MOF and MARA(CARS40)+1 种基金the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIPIAS04)the Central Guidance on Local Science and Technology Development Fund of Hebei Province,China(236Z6602G)。
文摘Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P<0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P<0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in shortside mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.
文摘胃肠道Cajal间质细胞（interstitialcells of Cajal，ICC）被称为胃肠动力的起搏者和调节者，是以刚状结构存在于胃肠道平滑肌层之间的一种特殊间质细胞，不同于平滑肌细胞和神经细胞,但具有神经信息传送给平滑肌细胞的功能，即肠神经系统释放的神经递质和肽类物质与ICC上的受体结合，激发ICC去极化，调控胃肠道平滑肌的舒缩功能。
