为探究^(60)Co-γ辐照对苦荞麸皮中黄酮类物质提取效率的影响,本研究采用不同剂量(0、6、12、18、24、30 k Gy)的^(60)Co-γ对苦荞麸皮进行辐照预处理,通过热回流法提取苦荞黄酮,比较^(60)Co-γ辐照对苦荞黄酮提取得率、主要黄酮类物质...为探究^(60)Co-γ辐照对苦荞麸皮中黄酮类物质提取效率的影响,本研究采用不同剂量(0、6、12、18、24、30 k Gy)的^(60)Co-γ对苦荞麸皮进行辐照预处理,通过热回流法提取苦荞黄酮,比较^(60)Co-γ辐照对苦荞黄酮提取得率、主要黄酮类物质单体含量和总含量、抑菌活性及苦荞麸皮微观结构的影响。结果表明,6~30 k Gy剂量的^(60)Co-γ辐照预处理可提高苦荞黄酮提取得率,其中12 k Gy组提取得率最高,为6.97%,较对照组的3.27%提高3.70个百分点;6~30 k Gy剂量的^(60)Co-γ辐照预处理可提高提取物中芦丁含量,12、18、30 k Gy剂量的预处理可提高提取物中烟花苷含量,其中12 k Gy组含量最高,芦丁和烟花苷含量分别为84.52、3.92 mg·g^(-1),较对照组的53.12、3.54 mg·g^(-1)分别提高59.11%与10.73%;6~24 k Gy剂量的^(60)Co-γ辐照预处理可提高苦荞主要黄酮总含量,其中12 k Gy组最高,为89.40 mg·g^(-1),较对照组的57.95 mg·g^(-1)提高54.27%。此外,^(60)Co-γ辐照可有效破坏苦荞麸皮组织结构,有助于苦荞黄酮的溶出;6~18 k Gy剂量的^(60)Co-γ辐照预处理增强了提取物对大肠杆菌的抑菌活性,其中18 k Gy剂量组的抑菌活性最强,抑菌圈直径达9.93 mm;12~24 k Gy剂量的^(60)Co-γ辐照预处理增强了提取物对金黄色葡萄球菌的抑菌活性,其中12 k Gy剂量组的抑菌活性最强,抑菌圈直径达10.30 mm。本研究可为苦荞黄酮的高效提取提供研究基础与技术参考。展开更多
[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ...[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ＇Favorita＇,and the genomic mutations in VM1 and VM2 gene rations in treatments with different doses were analyzed by SRAP markers.[Resalt] Thirty pairs of SRAP primers out of 88 pairs exhibited polymorphism with a rate of 34.1％ in the bulked selection of VM1 generation.A total of 225 bands were obtained,of which 64 were polymorphic with a rate of 28.4％.The polymorphism was reflected in the forms of deleted bands and added bands.Based on the result of the bulked selection of VM1 generation,25 primers with polymorphism were selected to scan VM2 generation.Five primers performed poorly,and nine of the rest 20 pairs revealed polymorphism and obtained nine polymorphic bands,of which only four bands were detected in VM1 generation and the other five ones were newly deleted bands.Only 9.8％ of the bands detected in VM1 generation were obtained in VM2 generation.Eventually,nine stable and dear polymorphic bands were recovered and cloned,and DNA sequences of six bands of them were acquired by sequencing.According to the comparative analysis,five fragments sequences were similar to potato chromosome with a similarity rate of 77％-89％,three of them located at the resistance gene cluster; another one fragment had a similarity of 93％ with some regions of the No.5 chromosome in tomato.[Conclusion] 60Co γ-ray irradiation can cause mutation of genomic DNA in potato;there is no significant correlation between the number of polymorphic bands and the irradiation dose; potymorphic bands are characterized by a larger number of deleted bands than that of the added ones.展开更多
文摘为探究^(60)Co-γ辐照对苦荞麸皮中黄酮类物质提取效率的影响,本研究采用不同剂量(0、6、12、18、24、30 k Gy)的^(60)Co-γ对苦荞麸皮进行辐照预处理,通过热回流法提取苦荞黄酮,比较^(60)Co-γ辐照对苦荞黄酮提取得率、主要黄酮类物质单体含量和总含量、抑菌活性及苦荞麸皮微观结构的影响。结果表明,6~30 k Gy剂量的^(60)Co-γ辐照预处理可提高苦荞黄酮提取得率,其中12 k Gy组提取得率最高,为6.97%,较对照组的3.27%提高3.70个百分点;6~30 k Gy剂量的^(60)Co-γ辐照预处理可提高提取物中芦丁含量,12、18、30 k Gy剂量的预处理可提高提取物中烟花苷含量,其中12 k Gy组含量最高,芦丁和烟花苷含量分别为84.52、3.92 mg·g^(-1),较对照组的53.12、3.54 mg·g^(-1)分别提高59.11%与10.73%;6~24 k Gy剂量的^(60)Co-γ辐照预处理可提高苦荞主要黄酮总含量,其中12 k Gy组最高,为89.40 mg·g^(-1),较对照组的57.95 mg·g^(-1)提高54.27%。此外,^(60)Co-γ辐照可有效破坏苦荞麸皮组织结构,有助于苦荞黄酮的溶出;6~18 k Gy剂量的^(60)Co-γ辐照预处理增强了提取物对大肠杆菌的抑菌活性,其中18 k Gy剂量组的抑菌活性最强,抑菌圈直径达9.93 mm;12~24 k Gy剂量的^(60)Co-γ辐照预处理增强了提取物对金黄色葡萄球菌的抑菌活性,其中12 k Gy剂量组的抑菌活性最强,抑菌圈直径达10.30 mm。本研究可为苦荞黄酮的高效提取提供研究基础与技术参考。
文摘[Objective] This study was to explore the mutagenic effect of 60Co γ-ray on Solanum tuberosum chromosomes.[Method] The 60Co γ-ray at different irradiation doses was applied to treat the minitubers of potato variety ＇Favorita＇,and the genomic mutations in VM1 and VM2 gene rations in treatments with different doses were analyzed by SRAP markers.[Resalt] Thirty pairs of SRAP primers out of 88 pairs exhibited polymorphism with a rate of 34.1％ in the bulked selection of VM1 generation.A total of 225 bands were obtained,of which 64 were polymorphic with a rate of 28.4％.The polymorphism was reflected in the forms of deleted bands and added bands.Based on the result of the bulked selection of VM1 generation,25 primers with polymorphism were selected to scan VM2 generation.Five primers performed poorly,and nine of the rest 20 pairs revealed polymorphism and obtained nine polymorphic bands,of which only four bands were detected in VM1 generation and the other five ones were newly deleted bands.Only 9.8％ of the bands detected in VM1 generation were obtained in VM2 generation.Eventually,nine stable and dear polymorphic bands were recovered and cloned,and DNA sequences of six bands of them were acquired by sequencing.According to the comparative analysis,five fragments sequences were similar to potato chromosome with a similarity rate of 77％-89％,three of them located at the resistance gene cluster; another one fragment had a similarity of 93％ with some regions of the No.5 chromosome in tomato.[Conclusion] 60Co γ-ray irradiation can cause mutation of genomic DNA in potato;there is no significant correlation between the number of polymorphic bands and the irradiation dose; potymorphic bands are characterized by a larger number of deleted bands than that of the added ones.
