Objective:To analyze the factors affecting the efficacy ofα-interferon in the treatment of chronic hepatitis B(CHB).Methods:A total of 100 patients with CHB treated in our hospital from June 2018 to June 2019 were se...Objective:To analyze the factors affecting the efficacy ofα-interferon in the treatment of chronic hepatitis B(CHB).Methods:A total of 100 patients with CHB treated in our hospital from June 2018 to June 2019 were selected.All patients were treated withα-interferon to evaluate the efficacy,and the factors affecting the effect ofα-interferon on CHB were analyzed.Results:After treatment,54 patients fully responded and 46 patients did not;the levels of white blood cells,HBV DNA,and HBsAg in the complete response group were lower than those in the incompletely response group,and the differences were statistically significant(P<0.05);Multivariate logistic regression analysis found that serum HBV DNA and HBsAg were independent factors affecting the efficacy ofα-interferon in the treatment of CHB(OR>1,P<0.05).Conclusion:Serum HBV DNA and HBsAg are risk factors that affect the efficacy ofα-interferon in the treatment of CHB.Monitoring the changes of serum HBV DNA and HBsAg levels has important clinical significance for predicting the efficacy.展开更多
Objective: To study the effect of α-interferon + gemcitabine combined with infusion chemotherapy on the proliferation and infiltrative growth of advanced bladder cancer. Methods: Patients with advanced bladder cancer...Objective: To study the effect of α-interferon + gemcitabine combined with infusion chemotherapy on the proliferation and infiltrative growth of advanced bladder cancer. Methods: Patients with advanced bladder cancer who accepted bladder infusion chemotherapy in Wuhan Red Cross Hospital between March 2015 and August 2016 were selected and randomly divided into the IFN-α+GEM group and the GEM group who acceptedα-interferon + gemcitabine combined with infusion chemotherapy and single gemcitabine infusion chemotherapy respectively. The contents of bladder cancer markers, cancer cell apoptosis molecules and angiogenesis molecules in urine were detected before treatment and 3 months after treatment. Results: 3 months after treatment, NMP-22, BLCA-4, Apo-A1, HA, CYFRA21-1, ANG, NAC-1, VEGF and bFGF levels in urine of both groups of patients were significantly lower than those before treatment while PTEN, TRAIL, Bim, HSG and p16 levels were significantly higher than those before treatment, and NMP-22, BLCA-4, Apo-A1, HA, CYFRA21-1, ANG, NAC-1, VEGF and bFGF levels in urine of IFN-α+GEM group were significantly lower than those of GEM group while PTEN, TRAIL, Bim, HSG and p16 levels were significantly higher than those of GEM group. Conclusion: α-interferon + gemcitabine combined with infusion chemotherapy can be more effective than single gemcitabine infusion chemotherapy in killing cancer cells and inhibiting the proliferation and infiltrative growth of the cancer cells.展开更多
Objective To study the effect of γ-interferon （IFNγ）, tumor necrosis factor related apoptosis inducing ligand （TRAIL）, and cisplatin or etoposide induced apoptosis in human neuroblastoma cell line SH-SY5Y and it...Objective To study the effect of γ-interferon （IFNγ）, tumor necrosis factor related apoptosis inducing ligand （TRAIL）, and cisplatin or etoposide induced apoptosis in human neuroblastoma cell line SH-SY5Y and its possible molecular mechanisms. Methods The expressions of Caspase 8 mRNA and protein were detected with RT-PCR and Western blot analysis. The effects of IFNγ, TRAIL, IFNγ ＋ TRAIL, IFNγ ＋ Caspase 8 inhibitor ＋ TRAIL, IFNγ ＋ cisplatin ＋ TRAIL, and IFNγ ＋ etoposide ＋ TRAIL on the growth and apoptosis of SH-SY5Y cells were detected with the methods of MTT and flow cytometry. The relative Caspase 8 activity was measured with colorimetric assay. Results Caspase 8 was undetectable in SH-SY5Y cells but an increased expression of Caspase 8 mRNA and protein was found after treatment with IFNγ. SH-SY5Y ceils themselves were not sensitive to TRAIL, but those expressing Caspase 8 after treatment with IFNγ were. The killing effect of TRAIL on SH-SY5Y cells expressing Caspase 8 was depressed by Caspase 8 inhibitor. Cisplatin and etoposide could enhance the sensitivity of TRAIL on SH-SY5Y cells. The relative Caspase 8 activity of SH-SY5Y cells in IFNγ ＋ TRAIL group was significantly higher than those of control group, IFNγ group, TRAIL group, and inhibitor group （ P 〈 0. 01 ）. There was no significant difference among IFNγ ＋ TRAIL group, IFNγ ＋ cisplatin ＋ TRAIL group, and IFNγ ＋ etoposide ＋ TRAIL group. Conclusions IFNγ could sensitize SH-SY5Y cells to TRAIL-induced apoptosis and this may be realized by the up-regulation of Caspase 8. Cisplatin and etoposide could enhance the killing effect of TRAIL on SH-SY5Y cells.展开更多
目的观察小儿消积止咳口服液治疗儿童慢性咳嗽的疗效及机制。方法选取慢性咳嗽患儿110例,随机分为对照组与观察组,每组55例。对照组患儿给予常规治疗,观察组患儿在常规治疗的基础上给予小儿消积止咳口服液治疗。另选取同期健康体检儿童5...目的观察小儿消积止咳口服液治疗儿童慢性咳嗽的疗效及机制。方法选取慢性咳嗽患儿110例,随机分为对照组与观察组,每组55例。对照组患儿给予常规治疗,观察组患儿在常规治疗的基础上给予小儿消积止咳口服液治疗。另选取同期健康体检儿童55例作为健康组。比较观察组与对照组的疗效,治疗前后评估咳嗽评分及匹兹堡睡眠质量指数(Pittsburgh sleep quality index,PSQI),检测治疗前后外周血嗜酸性粒细胞(eosinophils,EOS)、降钙素原(procalcitonin,PCT)、肿瘤坏死因子-α(tumor necrosisfactor-α,TNF-α)、干扰素-γ(interferon-γ,IFN-γ)、白细胞介素(interleukins,IL)-4(IL-4)、IL-8以及免疫球蛋白(immunoglobulin,Ig)A、IgG和IgM水平,检测肺功能包括用力肺活量(forced vital capacity,FVC)、第1秒用力呼气量百分比(the percentage of forced expiratory volume in the first second,FEV_(1)%)和呼气峰值流速(peak expiratory flow rate,PEF)。结果观察组的总有效率高于对照组,体温复常时间、肺啰音消失时间、咳痰消失时间及咳嗽消失时间均较对照组缩短,治疗后咳嗽评分及PSQI评分均低于对照组(P<0.05)。观察组和对照组治疗前的外周血EOS、PCT、TNF-α、IL-4和IL-8水平均高于健康组,IFN-γ、IgA、IgG和IgM水平均低于健康组(P<0.05);治疗后,观察组和对照组的外周血EOS、PCT、TNF-α、IL-4和IL-8水平均降低,IFN-γ、IgA、IgG和IgM水平均降低,FVC、FEV_(1)%和PEF均升高,且观察组上述指标均明显优于对照组(P<0.05)。结论外周血EOS、PCT、TNF-α、IL-4和IL-8水平在儿童慢性咳嗽中明显升高,IFN-γ水平明显降低,采用小儿消积止咳口服液治疗能够调节上述各指标平衡,提高临床疗效并促进症状缓解。展开更多
目的探讨重组α干扰素-2b和异丙托溴铵雾化吸入在小儿毛细支气管炎治疗中的临床效果。方法选取2020年4月至2022年8月湖州市中心医院诊治的112例毛细支气管炎患儿,根据信封法将其分为对照组和观察组,每组各56例。对照组患儿给予异丙托溴...目的探讨重组α干扰素-2b和异丙托溴铵雾化吸入在小儿毛细支气管炎治疗中的临床效果。方法选取2020年4月至2022年8月湖州市中心医院诊治的112例毛细支气管炎患儿,根据信封法将其分为对照组和观察组,每组各56例。对照组患儿给予异丙托溴铵雾化吸入,观察组患儿给予重组α干扰素-2b雾化吸入,两组患儿均连续治疗7d。比较两组患儿的肺功能、γ干扰素(interferon-γ,IFN-γ)、白细胞介素-10(interleukin-10,IL-10)、免疫功能及不良反应。结果干预7d后,观察组患儿的喘息、咳嗽、肺部湿啰音及痰鸣音消失时间均显著短于对照组(P<0.05);两组患儿的潮气量(tidal volume,TV)、潮气呼气峰流量(peak tidal expiratory flow,PTEF)及达峰时间比(ratio of time to peak tidal expiratory flow to total expiratory time,TPTEF/TE)、IFN-γ及IL-10均显著高于本组干预前(P<0.05),观察组患儿的TV、PTEF、TPTEF/TE、IFN-γ及IL-10水平均显著高于对照组(P<0.05);两组患儿的CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均显著高于本组干预前,CD8^(+)显著低于本组干预前(P<0.05);观察组患儿的CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均显著高于对照组,CD8^(+)显著低于对照组(P<0.05);两组患儿的不良反应发生率比较差异无统计学意义(χ^(2)=0.704,P=0.401)。结论毛细支气管炎患儿采用重组α干扰素-2b和异丙托溴铵雾化吸入均可获得良好的治疗效果,但前者有助于缩短症状消失时间,提高患儿免疫水平,值得临床推广应用。展开更多
文摘Objective:To analyze the factors affecting the efficacy ofα-interferon in the treatment of chronic hepatitis B(CHB).Methods:A total of 100 patients with CHB treated in our hospital from June 2018 to June 2019 were selected.All patients were treated withα-interferon to evaluate the efficacy,and the factors affecting the effect ofα-interferon on CHB were analyzed.Results:After treatment,54 patients fully responded and 46 patients did not;the levels of white blood cells,HBV DNA,and HBsAg in the complete response group were lower than those in the incompletely response group,and the differences were statistically significant(P<0.05);Multivariate logistic regression analysis found that serum HBV DNA and HBsAg were independent factors affecting the efficacy ofα-interferon in the treatment of CHB(OR>1,P<0.05).Conclusion:Serum HBV DNA and HBsAg are risk factors that affect the efficacy ofα-interferon in the treatment of CHB.Monitoring the changes of serum HBV DNA and HBsAg levels has important clinical significance for predicting the efficacy.
文摘Objective: To study the effect of α-interferon + gemcitabine combined with infusion chemotherapy on the proliferation and infiltrative growth of advanced bladder cancer. Methods: Patients with advanced bladder cancer who accepted bladder infusion chemotherapy in Wuhan Red Cross Hospital between March 2015 and August 2016 were selected and randomly divided into the IFN-α+GEM group and the GEM group who acceptedα-interferon + gemcitabine combined with infusion chemotherapy and single gemcitabine infusion chemotherapy respectively. The contents of bladder cancer markers, cancer cell apoptosis molecules and angiogenesis molecules in urine were detected before treatment and 3 months after treatment. Results: 3 months after treatment, NMP-22, BLCA-4, Apo-A1, HA, CYFRA21-1, ANG, NAC-1, VEGF and bFGF levels in urine of both groups of patients were significantly lower than those before treatment while PTEN, TRAIL, Bim, HSG and p16 levels were significantly higher than those before treatment, and NMP-22, BLCA-4, Apo-A1, HA, CYFRA21-1, ANG, NAC-1, VEGF and bFGF levels in urine of IFN-α+GEM group were significantly lower than those of GEM group while PTEN, TRAIL, Bim, HSG and p16 levels were significantly higher than those of GEM group. Conclusion: α-interferon + gemcitabine combined with infusion chemotherapy can be more effective than single gemcitabine infusion chemotherapy in killing cancer cells and inhibiting the proliferation and infiltrative growth of the cancer cells.
基金the National Natural Sciences Foundation of China(39470739)the Ministry of Public Health Research Foundation(20122167)the Doctor Startup-Natural Science Foundation of Li-aoning Province (20041047)
文摘Objective To study the effect of γ-interferon （IFNγ）, tumor necrosis factor related apoptosis inducing ligand （TRAIL）, and cisplatin or etoposide induced apoptosis in human neuroblastoma cell line SH-SY5Y and its possible molecular mechanisms. Methods The expressions of Caspase 8 mRNA and protein were detected with RT-PCR and Western blot analysis. The effects of IFNγ, TRAIL, IFNγ ＋ TRAIL, IFNγ ＋ Caspase 8 inhibitor ＋ TRAIL, IFNγ ＋ cisplatin ＋ TRAIL, and IFNγ ＋ etoposide ＋ TRAIL on the growth and apoptosis of SH-SY5Y cells were detected with the methods of MTT and flow cytometry. The relative Caspase 8 activity was measured with colorimetric assay. Results Caspase 8 was undetectable in SH-SY5Y cells but an increased expression of Caspase 8 mRNA and protein was found after treatment with IFNγ. SH-SY5Y ceils themselves were not sensitive to TRAIL, but those expressing Caspase 8 after treatment with IFNγ were. The killing effect of TRAIL on SH-SY5Y cells expressing Caspase 8 was depressed by Caspase 8 inhibitor. Cisplatin and etoposide could enhance the sensitivity of TRAIL on SH-SY5Y cells. The relative Caspase 8 activity of SH-SY5Y cells in IFNγ ＋ TRAIL group was significantly higher than those of control group, IFNγ group, TRAIL group, and inhibitor group （ P 〈 0. 01 ）. There was no significant difference among IFNγ ＋ TRAIL group, IFNγ ＋ cisplatin ＋ TRAIL group, and IFNγ ＋ etoposide ＋ TRAIL group. Conclusions IFNγ could sensitize SH-SY5Y cells to TRAIL-induced apoptosis and this may be realized by the up-regulation of Caspase 8. Cisplatin and etoposide could enhance the killing effect of TRAIL on SH-SY5Y cells.
文摘目的观察小儿消积止咳口服液治疗儿童慢性咳嗽的疗效及机制。方法选取慢性咳嗽患儿110例,随机分为对照组与观察组,每组55例。对照组患儿给予常规治疗,观察组患儿在常规治疗的基础上给予小儿消积止咳口服液治疗。另选取同期健康体检儿童55例作为健康组。比较观察组与对照组的疗效,治疗前后评估咳嗽评分及匹兹堡睡眠质量指数(Pittsburgh sleep quality index,PSQI),检测治疗前后外周血嗜酸性粒细胞(eosinophils,EOS)、降钙素原(procalcitonin,PCT)、肿瘤坏死因子-α(tumor necrosisfactor-α,TNF-α)、干扰素-γ(interferon-γ,IFN-γ)、白细胞介素(interleukins,IL)-4(IL-4)、IL-8以及免疫球蛋白(immunoglobulin,Ig)A、IgG和IgM水平,检测肺功能包括用力肺活量(forced vital capacity,FVC)、第1秒用力呼气量百分比(the percentage of forced expiratory volume in the first second,FEV_(1)%)和呼气峰值流速(peak expiratory flow rate,PEF)。结果观察组的总有效率高于对照组,体温复常时间、肺啰音消失时间、咳痰消失时间及咳嗽消失时间均较对照组缩短,治疗后咳嗽评分及PSQI评分均低于对照组(P<0.05)。观察组和对照组治疗前的外周血EOS、PCT、TNF-α、IL-4和IL-8水平均高于健康组,IFN-γ、IgA、IgG和IgM水平均低于健康组(P<0.05);治疗后,观察组和对照组的外周血EOS、PCT、TNF-α、IL-4和IL-8水平均降低,IFN-γ、IgA、IgG和IgM水平均降低,FVC、FEV_(1)%和PEF均升高,且观察组上述指标均明显优于对照组(P<0.05)。结论外周血EOS、PCT、TNF-α、IL-4和IL-8水平在儿童慢性咳嗽中明显升高,IFN-γ水平明显降低,采用小儿消积止咳口服液治疗能够调节上述各指标平衡,提高临床疗效并促进症状缓解。
文摘目的探讨重组α干扰素-2b和异丙托溴铵雾化吸入在小儿毛细支气管炎治疗中的临床效果。方法选取2020年4月至2022年8月湖州市中心医院诊治的112例毛细支气管炎患儿,根据信封法将其分为对照组和观察组,每组各56例。对照组患儿给予异丙托溴铵雾化吸入,观察组患儿给予重组α干扰素-2b雾化吸入,两组患儿均连续治疗7d。比较两组患儿的肺功能、γ干扰素(interferon-γ,IFN-γ)、白细胞介素-10(interleukin-10,IL-10)、免疫功能及不良反应。结果干预7d后,观察组患儿的喘息、咳嗽、肺部湿啰音及痰鸣音消失时间均显著短于对照组(P<0.05);两组患儿的潮气量(tidal volume,TV)、潮气呼气峰流量(peak tidal expiratory flow,PTEF)及达峰时间比(ratio of time to peak tidal expiratory flow to total expiratory time,TPTEF/TE)、IFN-γ及IL-10均显著高于本组干预前(P<0.05),观察组患儿的TV、PTEF、TPTEF/TE、IFN-γ及IL-10水平均显著高于对照组(P<0.05);两组患儿的CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均显著高于本组干预前,CD8^(+)显著低于本组干预前(P<0.05);观察组患儿的CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均显著高于对照组,CD8^(+)显著低于对照组(P<0.05);两组患儿的不良反应发生率比较差异无统计学意义(χ^(2)=0.704,P=0.401)。结论毛细支气管炎患儿采用重组α干扰素-2b和异丙托溴铵雾化吸入均可获得良好的治疗效果,但前者有助于缩短症状消失时间,提高患儿免疫水平,值得临床推广应用。
