Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins ext...Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin.Methods:The phytochemicol tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents.Diabetes was induced in rats by intraperitoneal(i.p.) injection of streptozotocin(STZ) at a dose of 65 mg/kg bw.The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips(Dextrostix,Bayer Diagnostics).Blood samples were taken by cutting the tip of the tail.Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method.Results:Administration of 62.5 and 2S.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity(32.33%and 40.17%respectively) during the first week.However,diabetic group treated with saponin extract produced a maximum fall of 73.1%and 76.03%at day 1 and day 21 compared to the diabetics control.Also,blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79%on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters(20.77%-49.00%).The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions:These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased,significantly,consequently this plant might be of value in diabetes treatment.展开更多
Aim: Whole body vibration (WBV) is thought to improve blood flow and autonomic balance and thereby induce a relaxation effect, which suggests its use for stress management. However, the relaxation effect of WBV traini...Aim: Whole body vibration (WBV) is thought to improve blood flow and autonomic balance and thereby induce a relaxation effect, which suggests its use for stress management. However, the relaxation effect of WBV training has not been objectively evaluated thus far. The purpose of this study was to determine the biological response to WBV training by measuring peripheral blood flow and salivary components using non-invasive techniques. Methods: Participants included 10 healthy volunteers (7 men, 3 women;mean age 33.8 ± 2.3) who provided oral consent and served as their own control. Each participant performed 15 types of stretching exercises for 10.5 min on the Power Plate? and cutaneous blood flow and salivary components were measured before and after the exercise. One week later, all participants performed the same exercise regimen for 10.5 min on a non-vibratory plate, and blood flow measurement and salivary tests were performed in a similar manner. Cutaneous blood flow was measured in the 4th digit for 1 min using the laser speckle flowgraphy. Saliva samples were evaluated for cortisol levels and α-amylase activity. To determine the effects of stretching exercises on the Power Plate? vs a non-vibratory plate, the differences in pre- and post-exercise peripheral blood flow, salivary cortisol levels, and salivary α-amylase activity were statistically evaluated by the t-test. Results: Mean blood flow before and after the exercise on the Power Plate? was 122.0 ± 54.2 and 156.7 ± 51.2, respectively;on a non-vibratory plate, blood flow was 136.6 ± 47.9 and 146.3 ± 38.3, respectively. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.215). Mean cortisol levels before and after the exercise on the Power Plate? were 266.6 ± 125 and 204.9 ± 61.6, respectively;on a non-vibratory plate, the levels were 439.0 ± 121.7 and 425.8 ± 118.8, respectively. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.384). Mean α-amylase activity before and after the exercise on the Power Plate? was 3.74 ± 2.89 and 5.40 ± 3.76, respectively;on a non-vibratory plate, the activity was 3.95 ± 2.23 and 3.28 ± 1.73. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.115). Conclusion: Our results showed that a brief WBV training increased peripheral blood flow, reduced cortisol levels, and increased α-amylase activity. WBV appears to regulate autonomic activity, in particular, suppress sympathetic activity and improve bodily functions. Thus WBV exercise may be conductive for stress management, but further investigation is warranted to determine the optimal duration of WBV training for stress relief.展开更多
Objective:To study the antibacterial and antiamylase properties of methanol and acetone extracts of nudibranchs including Bursatella leachii(B.leachii),Kalinga ornata(K.ornata),Aplysia sp.Methods:Crude methanol and ac...Objective:To study the antibacterial and antiamylase properties of methanol and acetone extracts of nudibranchs including Bursatella leachii(B.leachii),Kalinga ornata(K.ornata),Aplysia sp.Methods:Crude methanol and acetone extracts of sea slugs were tested for inhibition of fish bacterial pathogens'growth through disc diffusion method.The activity was measured based on the formation of inhibition zone around the disc impregnated with crude extracts.Theα-amylase inhibitory effect was also measured calorimetrically.The chemical fingerprinting of the extract was recorded with HPTLC and GC-MS.Results:The solvent extracts of all the three sea slugs showed antibacterial property.The maximum zone of inhibition(>15-20 mm)was recorded for methanol and acetone extracts of K.ornata.The methanol extract of Aplysia sp.exhibited 93%inhibition againstα-amylase,following by B.leachii(methanol)70.6%and K.ornata(methanol)49.03%inhibition respectively.The acetone extracts didn't show any notable inhibition.The presence of free amino acids like lysine,aspartic acid,glutamic acid,arginine etc.,terpenoids and pigents were confirmed through HPTLC analysis.The presence of siloxanes and propanoic acid were also revealed through GC-MS.Conclusions:This study suggests that further scrutinisation of the B.leachii,K.ornata and Aplysia sp.will pave the way for development of antibacterial andα-amylase inhibitory agent for therapeutic application.展开更多
文摘Objective:To achieve a primary pharmacological screening contained in the aqueous extract of Berberis vulgaris(B.vulgaris) and to examine the hypoglycaemic effect and biochemical parameters of aqueous and saponins extract on groups of rats rendered diabetic by injection of streptozotocin.Methods:The phytochemicol tests to detect the presence of different compounds were based on the visual observation of color change or formation of precipitate after the addition of specific reagents.Diabetes was induced in rats by intraperitoneal(i.p.) injection of streptozotocin(STZ) at a dose of 65 mg/kg bw.The fasting blood glucose levels were estimated by glucose oxidase-peroxidase reactive strips(Dextrostix,Bayer Diagnostics).Blood samples were taken by cutting the tip of the tail.Serum cholesterol and serum triglycerides were estimated by enzymatic DHBS colorimetric method.Results:Administration of 62.5 and 2S.0 mg/kg of saponins and aqueous extract respectively in normal rats group shows a significant hypoglycemic activity(32.33%and 40.17%respectively) during the first week.However,diabetic group treated with saponin extract produced a maximum fall of 73.1%and 76.03%at day 1 and day 21 compared to the diabetics control.Also,blood glucose levels of the diabetic rats treated with aqueous extract showed decrease of 78.79%on the first day and the effect remains roughly constant during 3 week. Both extracts also declined significantly biochemical parameters(20.77%-49.00%).The control in the loss of body weight was observed in treated diabetic rats as compared to diabetic controls. Conclusions:These results demonstrated significant antidiabetic effects and showed that serum cholesterol and serum triglycerides levels were decreased,significantly,consequently this plant might be of value in diabetes treatment.
文摘Aim: Whole body vibration (WBV) is thought to improve blood flow and autonomic balance and thereby induce a relaxation effect, which suggests its use for stress management. However, the relaxation effect of WBV training has not been objectively evaluated thus far. The purpose of this study was to determine the biological response to WBV training by measuring peripheral blood flow and salivary components using non-invasive techniques. Methods: Participants included 10 healthy volunteers (7 men, 3 women;mean age 33.8 ± 2.3) who provided oral consent and served as their own control. Each participant performed 15 types of stretching exercises for 10.5 min on the Power Plate? and cutaneous blood flow and salivary components were measured before and after the exercise. One week later, all participants performed the same exercise regimen for 10.5 min on a non-vibratory plate, and blood flow measurement and salivary tests were performed in a similar manner. Cutaneous blood flow was measured in the 4th digit for 1 min using the laser speckle flowgraphy. Saliva samples were evaluated for cortisol levels and α-amylase activity. To determine the effects of stretching exercises on the Power Plate? vs a non-vibratory plate, the differences in pre- and post-exercise peripheral blood flow, salivary cortisol levels, and salivary α-amylase activity were statistically evaluated by the t-test. Results: Mean blood flow before and after the exercise on the Power Plate? was 122.0 ± 54.2 and 156.7 ± 51.2, respectively;on a non-vibratory plate, blood flow was 136.6 ± 47.9 and 146.3 ± 38.3, respectively. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.215). Mean cortisol levels before and after the exercise on the Power Plate? were 266.6 ± 125 and 204.9 ± 61.6, respectively;on a non-vibratory plate, the levels were 439.0 ± 121.7 and 425.8 ± 118.8, respectively. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.384). Mean α-amylase activity before and after the exercise on the Power Plate? was 3.74 ± 2.89 and 5.40 ± 3.76, respectively;on a non-vibratory plate, the activity was 3.95 ± 2.23 and 3.28 ± 1.73. The differences in pre-exercise and post-exercise values of the two training methods were not significant (p = 0.115). Conclusion: Our results showed that a brief WBV training increased peripheral blood flow, reduced cortisol levels, and increased α-amylase activity. WBV appears to regulate autonomic activity, in particular, suppress sympathetic activity and improve bodily functions. Thus WBV exercise may be conductive for stress management, but further investigation is warranted to determine the optimal duration of WBV training for stress relief.
基金Supported by Ministry of Earth Sciences(MoES-G4/12319)under the‘Drugs from the Sea’programme.
文摘Objective:To study the antibacterial and antiamylase properties of methanol and acetone extracts of nudibranchs including Bursatella leachii(B.leachii),Kalinga ornata(K.ornata),Aplysia sp.Methods:Crude methanol and acetone extracts of sea slugs were tested for inhibition of fish bacterial pathogens'growth through disc diffusion method.The activity was measured based on the formation of inhibition zone around the disc impregnated with crude extracts.Theα-amylase inhibitory effect was also measured calorimetrically.The chemical fingerprinting of the extract was recorded with HPTLC and GC-MS.Results:The solvent extracts of all the three sea slugs showed antibacterial property.The maximum zone of inhibition(>15-20 mm)was recorded for methanol and acetone extracts of K.ornata.The methanol extract of Aplysia sp.exhibited 93%inhibition againstα-amylase,following by B.leachii(methanol)70.6%and K.ornata(methanol)49.03%inhibition respectively.The acetone extracts didn't show any notable inhibition.The presence of free amino acids like lysine,aspartic acid,glutamic acid,arginine etc.,terpenoids and pigents were confirmed through HPTLC analysis.The presence of siloxanes and propanoic acid were also revealed through GC-MS.Conclusions:This study suggests that further scrutinisation of the B.leachii,K.ornata and Aplysia sp.will pave the way for development of antibacterial andα-amylase inhibitory agent for therapeutic application.
